Q01758 (ISP2_OSMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Type-2 ice-structuring protein Alternative name(s): Type II antifreeze protein Short name=AFP
Organism	Osmerus mordax (Rainbow smelt) (Atherina mordax)
Taxonomic identifier	8014 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Protacanthopterygii › Osmeriformes › Osmeroidei › Osmeroidea › Osmeridae › Osmerinae › Osmerini › Osmerus

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Antifreeze proteins lower the blood freezing point.
Subcellular location	Secreted.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Lectin
Molecular function	Antifreeze protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	response to freezing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 33	17	Potential		PRO_0000017546
	Chain	34 – 175	142	Type-2 ice-structuring protein		PRO_0000017547
Regions
	Domain	36 – 163	128	C-type lectin
Amino acid modifications
	Disulfide bond	38 ↔ 49		By similarity
	Disulfide bond	66 ↔ 159		By similarity
	Disulfide bond	103 ↔ 134		By similarity
	Disulfide bond	123 ↔ 145		By similarity
	Disulfide bond	135 ↔ 151		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01758 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E2A72D8C030AFAA3	FASTA	175	19,054
	10 20 30 40 50 60 MLAALLVCAM VALTRAANGD TGKEAVMTGS SGKNLTECPT DWKMFNGRCF LFNPLQLHWA 70 80 90 100 110 120 HAQISCMKDG ANLASIHSLE EYAFVKELTT AGLIPAWIGG SDCHVSTYWF WMDSTSMDFT 130 140 150 160 170 DWCAAQPDFT LTECCIQINV GVGKCWNDTP CTHLHASVCA KPATVIPEVT PPSIM

References

[1]

"Structural and functional similarity between fish antifreeze proteins and calcium-dependent lectins." Ewart K.V., Rubinsky B., Fletcher G.L. Biochem. Biophys. Res. Commun. 185:335-340(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 49-65; 69-78; 145-154 AND 161-173. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96154 mRNA. Translation: AAA49442.1.
PIR	JH0626.
3D structure databases
ProteinModelPortal	Q01758.
SMR	Q01758. Positions 38-162.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG004151.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR002353. AntifreezeII. IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
PRINTS	PR00356. ANTIFREEZEII.
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISP2_OSMMO

Accession

Primary (citable) accession number: Q01758

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program