Q01722 (GCR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycolytic genes transcriptional activator GCR2

Gene names

Name:	GCR2
Ordered Locus Names:	YNL199C
ORF Names:	N1374

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator required for the expression of glycolytic genes. Enhances the CT box-dependent transcriptional activation of a RAP1-GCR1 complex. Required for GCR1 phosphorylation. Ref.1 Ref.5 Ref.6
Subunit structure	Homodimer via the leucine-zipper domain. Forms a complex with a GCR1 homodimer. Ref.6
Subcellular location	Nucleus Probable.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.6. Source: SGD regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.6PubMed 2247062. Source: SGD
Cellular_component	nuclear envelope Inferred from direct assay PubMed 15817685. Source: SGD
Molecular_function	RNA polymerase II activating transcription factor binding Inferred from direct assay Ref.6. Source: SGD RNA polymerase II transcription factor binding transcription factor activity Inferred from genetic interaction Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Glycolytic genes transcriptional activator GCR2		PRO_0000087446
Regions
	Region	497 – 534	38	Leucine-zipper
	Motif	281 – 288	8	Nuclear localization signal Potential
	Compositional bias	255 – 283	29	Asn-rich
Amino acid modifications
	Modified residue	151	1	Phosphoserine Ref.7
	Modified residue	348	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q01722 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 958D4A9393255B1B	FASTA	534	58,062
	10 20 30 40 50 60 MHHQTKLDVF IIRAYNLLSN ESVISGASLQ SVTNSPQTTT NTPSGMVNGA VGTGIANPTG 70 80 90 100 110 120 LMGSDSTPNI DEIITSTGSN ALTKTNSDSA NGTPNGNSSS TSAISNASNP ATTGNNASSS 130 140 150 160 170 180 ATSNGIYTQA QYSQLFAKIS KLYNATLSSG SIDDRSTSPK SAIELYQRFQ QMIKELELSF 190 200 210 220 230 240 DASPYAKYFR RLDGRLWQIK TDSELENDEL WRLVSMSIFT VFDPQTGQIL TQGRRKGNSL 250 260 270 280 290 300 NTSTKGSPSD LQGINNGNNN GNNGNIGNGS NIKNYGNKNM PNNRTKKRGT RVAKNAKNGK 310 320 330 340 350 360 NNKNSNKERN GITDTSAFSN TTISNPGTNM LFDPSLSQQL QKRLQTLSQD VNSRSLTGYY 370 380 390 400 410 420 TQPTSPGSGG FEFGLSHADL NPNASSNTMG YNTMSNNGSH SWKRRSLGSL DVNTLDDEAV 430 440 450 460 470 480 EELLQLTNTS KRQRPMTTAA EGALINDGPD TNLNANNTQM KVDLNPSNSM GPIDTEAVIR 490 500 510 520 530 PLKEAYDAII SEKGQRIVQL ERELELQRQE TQWLRKMLIE DMGCVRSMLR DLQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Role of GCR2 in transcriptional activation of yeast glycolytic genes." Uemura H., Jigami Y. Mol. Cell. Biol. 12:3834-3842(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH GCR1.
[2]	"A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function." Jonniaux J.-L., Coster F., Purnelle B., Goffeau A. Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Specialized Rap1p/Gcr1p transcriptional activation through Gcr1p DNA contacts requires Gcr2p, as does hyperphosphorylation of Gcr1p." Zeng X., Deminoff S.J., Santangelo G.M. Genetics 147:493-505(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Rap1p requires Gcr1p and Gcr2p homodimers to activate ribosomal protein and glycolytic genes, respectively." Deminoff S.J., Santangelo G.M. Genetics 158:133-143(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, HOMODIMERIZATION.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-348, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10104 Genomic DNA. Translation: BAA00985.1. X78898 Genomic DNA. Translation: CAA55509.1. Z71475 Genomic DNA. Translation: CAA96097.1. BK006947 Genomic DNA. Translation: DAA10355.1.
PIR	S31300.
RefSeq	NP_014200.1. NM_001183037.1.
3D structure databases
ProteinModelPortal	Q01722.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-732N.
IntAct	Q01722. 10 interactions.
MINT	MINT-400852.
STRING	4932.YNL199C.
Proteomic databases
PaxDb	Q01722.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL199C; YNL199C; YNL199C.
GeneID	855522.
KEGG	sce:YNL199C.
Organism-specific databases
CYGD	YNL199c.
SGD	S000005143. GCR2.
Phylogenomic databases
eggNOG	NOG42464.
HOGENOM	HOG000000851.
OMA	MDETISE.
OrthoDB	EOG43JGF5.
Gene expression databases
Genevestigator	Q01722.
GermOnline	YNL199C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979556.

Entry information

Entry name

GCR2_YEAST

Accession

Primary (citable) accession number: Q01722
Secondary accession number(s): D6W0Y9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program