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Protein

Nitrate reductase

Gene

niaD

Organism
Penicillium chrysogenum (Penicillium notatum)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADSAAS annotation
  • Mo-molybdopterinSAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseSAAS annotation

Keywords - Biological processi

Nitrate assimilationUniRule annotation

Keywords - Ligandi

FADSAAS annotation, Flavoprotein, HemeSAAS annotation, Iron, Metal-binding, MolybdenumSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductaseUniRule annotation
Gene namesi
Name:niaDImported
OrganismiPenicillium chrysogenum (Penicillium notatum)Imported
Taxonomic identifieri5076 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicilliumPenicillium chrysogenum complex

Interactioni

Subunit structurei

Homodimer.SAAS annotation

Protein-protein interaction databases

STRINGi500485.XP_002559559.1.

Structurei

3D structure databases

ProteinModelPortaliQ01696.
SMRiQ01696. Positions 37-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini511 – 58676Cytochrome b5 heme-bindingInterPro annotationAdd
BLAST
Domaini612 – 723112FAD-binding FR-typeInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nitrate reductase family.UniRule annotation
Contains FAD-binding FR-type domain.SAAS annotation
Contains cytochrome b5 heme-binding domain.SAAS annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASITTQTIT QPETISFKNA QITVEDIIPT PGEPDIPLPP PSKNPVDILD
60 70 80 90 100
VDKGTPDNHV PRDPRLIRLT GVHPFNVEPP LTALYKEGFL TSPELFYVRN
110 120 130 140 150
HGPVPRVRDE DIPNWEISIE GLVEKPLVLN FREVLQKYDQ ITAPITLVCA
160 170 180 190 200
GNRRKEQNTV RKSKGFSWGA AGLSTALFTG PMMADILRSA KPLRQAKYVC
210 220 230 240 250
MEGADKLPNG FYGTSVKLNW AMDPNRGIML AHKMNGEDLR PDHGSPLRAV
260 270 280 290 300
VPGQIGGRSV KWLKKLILTD APSDNWYHIN DNRVLPTMVT PEMSAENRKW
310 320 330 340 350
WTDERYAIYE LNVNSVAVYP QHEEEIDLST AGPTYTVKGY AYAGGGRRIT
360 370 380 390 400
KVEVSLDRGK SWHLSEIEYA EDKYRDFDGE LFGGKVDMWW REASFCWSFW
410 420 430 440 450
SLDVPVADLE TSDAILVRAV DEALSAIPRD MYWSVLGMMN NPWFRVAITK
460 470 480 490 500
QGNTLKFEHP THPAKAGGWM ERVKKTGGDL LNGNWGERVE GEEPIEPEPV
510 520 530 540 550
KEINMKKDGL NRQIDLQELK ANSSSEKPWF VVNGEVYDGT AFLEGHPGGA
560 570 580 590 600
ISVTSSAGLD VSEDFLAIHS ETAKAMMPDY HIGTLDKASL EALKGDSTAG
610 620 630 640 650
SDVPQAVFLQ SGKWTKATLT EKKDVSWDTR LFVFDLEHEK QTLGLPIGQH
660 670 680 690 700
LMIKVQDPSN NEAIIRSYTP MSDTNLIGKM ELLVKIYFPT ESIPGGKMTM
710 720 730 740 750
ALDKLPLGSK IDCKGPTGRF EYLGNGRVSI SGKERHVRSF KMICGGTGIT
760 770 780 790 800
PVFQVLRAVM QDTQDPTTCV VLDGNRQEED ILCRSDLDAY VETDSQKCTV
810 820 830 840 850
VHTLTKGSDT WTGHRGRISE ELLAEYAAPE EQSMVLVCGP GPMEKSAREI
860
LLAQGWAESD LHFF
Length:864
Mass (Da):96,250
Last modified:November 1, 1996 - v1
Checksum:i9187C3D63AAF1E8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20779 Genomic DNA. Translation: AAB03900.1.
PIRiS57261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20779 Genomic DNA. Translation: AAB03900.1.
PIRiS57261.

3D structure databases

ProteinModelPortaliQ01696.
SMRiQ01696. Positions 37-488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi500485.XP_002559559.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "NRE, the major nitrogen regulatory protein of Penicillium chrysogenum, binds specifically to elements in the intergenic promoter regions of nitrate assimilation and penicillin biosynthetic gene clusters."
    Haas H., Marzluf G.A.
    Curr. Genet. 28:177-183(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Haas H.H.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiQ01696_PENCH
AccessioniPrimary (citable) accession number: Q01696
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.