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Q01682

- PPA2_SCHPO

UniProt

Q01682 - PPA2_SCHPO

Protein

Thiamine-repressible acid phosphatase pho4

Gene

pho4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    May dephosphorylate thiamine phosphates.

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei69 – 691NucleophileBy similarity
    Active sitei341 – 3411Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: PomBase

    GO - Biological processi

    1. cellular response to vitamin B1 starvation Source: PomBase
    2. dephosphorylation Source: PomBase
    3. thiamine biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamine-repressible acid phosphatase pho4 (EC:3.1.3.2)
    Gene namesi
    Name:pho4
    ORF Names:SPBC428.03c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC428.03c.

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall-bounded periplasmic space Source: PomBase
    2. endoplasmic reticulum Source: PomBase
    3. external side of plasma membrane Source: PomBase
    4. extracellular region Source: PomBase
    5. fungal-type cell wall Source: PomBase
    6. Golgi apparatus Source: PomBase

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18181 PublicationAdd
    BLAST
    Chaini19 – 463445Thiamine-repressible acid phosphatase pho4PRO_0000023952Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ01682.
    PaxDbiQ01682.

    Expressioni

    Inductioni

    Repressed by thiamine.

    Interactioni

    Protein-protein interaction databases

    BioGridi277132. 12 interactions.
    MINTiMINT-4693366.
    STRINGi4896.SPBC428.03c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01682.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histidine acid phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG260296.
    HOGENOMiHOG000115655.
    KOiK01078.
    OMAiCETHATK.
    OrthoDBiEOG7TQV9N.
    PhylomeDBiQ01682.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    IPR016274. Histidine_acid_Pase_euk.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
    SUPFAMiSSF53254. SSF53254. 1 hit.
    PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01682-1 [UniParc]FASTAAdd to Basket

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    MKLSGISLWL LAASIVHAGK SQFEAFENEF YFKDHLGTIS VYHEPYFNGP    50
    TTSFPESCAI KQVHLLQRHG SRNPTGDDTA TDVSSAQYID IFQNKLLNGS 100
    IPVNFSYPEN PLYFVKHWTP VIKAENADQL SSSGRIELFD LGRQVFERYY 150
    ELFDTDVYDI NTAAQERVVD SAEWFSYGMF GDDMQNKTNF IVLPEDDSAG 200
    ANSLAMYYSC PVYEDNNIDE NTTEAAHTSW RNVFLKPIAN RLNKYFDSGY 250
    NLTVSDVRSL YYICVYEIAL RDNSDFCSLF TPSEFLNFEY DSDLDYAYWG 300
    GPASEWASTL GGAYVNNLAN NLRKGVNNAS DRKVFLAFTH DSQIIPVEAA 350
    LGFFPDITPE HPLPTDKNIF TYSLKTSSFV PFAGNLITEL FLCSDNKYYV 400
    RHLVNQQVYP LTDCGYGPSG ASDGLCELSA YLNSSVRVNS TSNGIANFNS 450
    QCQAHSTNVT VYY 463
    Length:463
    Mass (Da):52,119
    Last modified:July 1, 1993 - v1
    Checksum:iF48EAFF8BB6B234A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56939 Genomic DNA. Translation: CAA40258.1.
    CU329671 Genomic DNA. Translation: CAA22278.1.
    AB027777 Genomic DNA. Translation: BAA87081.1.
    PIRiS14119.
    RefSeqiNP_595181.1. NM_001021089.2.

    Genome annotation databases

    EnsemblFungiiSPBC428.03c.1; SPBC428.03c.1:pep; SPBC428.03c.
    GeneIDi2540606.
    KEGGispo:SPBC428.03c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56939 Genomic DNA. Translation: CAA40258.1 .
    CU329671 Genomic DNA. Translation: CAA22278.1 .
    AB027777 Genomic DNA. Translation: BAA87081.1 .
    PIRi S14119.
    RefSeqi NP_595181.1. NM_001021089.2.

    3D structure databases

    ProteinModelPortali Q01682.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277132. 12 interactions.
    MINTi MINT-4693366.
    STRINGi 4896.SPBC428.03c-1.

    Proteomic databases

    MaxQBi Q01682.
    PaxDbi Q01682.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC428.03c.1 ; SPBC428.03c.1:pep ; SPBC428.03c .
    GeneIDi 2540606.
    KEGGi spo:SPBC428.03c.

    Organism-specific databases

    PomBasei SPBC428.03c.

    Phylogenomic databases

    eggNOGi NOG260296.
    HOGENOMi HOG000115655.
    KOi K01078.
    OMAi CETHATK.
    OrthoDBi EOG7TQV9N.
    PhylomeDBi Q01682.

    Miscellaneous databases

    NextBioi 20801731.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    IPR016274. Histidine_acid_Pase_euk.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000894. Acid_phosphatase. 1 hit.
    SUPFAMi SSF53254. SSF53254. 1 hit.
    PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The structural gene coding for thiamin-repressible acid phosphatase in Schizosaccharomyces pombe."
      Yang J., Schweingruber M.E.
      Curr. Genet. 18:269-272(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-30.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-71.
      Strain: ATCC 38364 / 968.

    Entry informationi

    Entry nameiPPA2_SCHPO
    AccessioniPrimary (citable) accession number: Q01682
    Secondary accession number(s): Q9UU70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3