Thiamine-repressible acid phosphatase pho4 precursor - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

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Q01682 (PPA2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Thiamine-repressible acid phosphatase pho4
EC=3.1.3.2

Gene names

Name:	pho4
ORF Names:	SPBC428.03c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May dephosphorylate thiamine phosphates.
Catalytic activity	A phosphate monoester + H₂O = an alcohol + phosphate.
Subcellular location	Secreted › cell wall.
Induction	Repressed by thiamine.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to vitamin B1 starvation Inferred from mutant phenotype PubMed 7499352. Source: PomBase thiamine biosynthetic process Inferred from mutant phenotype Ref.1. Source: PomBase
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 16823372. Source: PomBase cell wall-bounded periplasmic space Inferred from sequence orthology. Source: PomBase endoplasmic reticulum Inferred from direct assay PubMed 16823372. Source: PomBase external side of plasma membrane Non-traceable author statement. Source: PomBase extracellular region Inferred from sequence orthology. Source: PomBase fungal-type cell wall Inferred from sequence orthology. Source: PomBase
Molecular_function	acid phosphatase activity Inferred from mutant phenotype Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

18

Chain

445

Thiamine-repressible acid phosphatase pho4

PRO_0000023952

Sites

Active site

1

Nucleophile By similarity

Active site

1

Proton donor By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q01682 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: F48EAFF8BB6B234A
Show »

463

52,119

        10         20         30         40         50         60 
MKLSGISLWL LAASIVHAGK SQFEAFENEF YFKDHLGTIS VYHEPYFNGP TTSFPESCAI 

        70         80         90        100        110        120 
KQVHLLQRHG SRNPTGDDTA TDVSSAQYID IFQNKLLNGS IPVNFSYPEN PLYFVKHWTP 

       130        140        150        160        170        180 
VIKAENADQL SSSGRIELFD LGRQVFERYY ELFDTDVYDI NTAAQERVVD SAEWFSYGMF 

       190        200        210        220        230        240 
GDDMQNKTNF IVLPEDDSAG ANSLAMYYSC PVYEDNNIDE NTTEAAHTSW RNVFLKPIAN 

       250        260        270        280        290        300 
RLNKYFDSGY NLTVSDVRSL YYICVYEIAL RDNSDFCSLF TPSEFLNFEY DSDLDYAYWG 

       310        320        330        340        350        360 
GPASEWASTL GGAYVNNLAN NLRKGVNNAS DRKVFLAFTH DSQIIPVEAA LGFFPDITPE 

       370        380        390        400        410        420 
HPLPTDKNIF TYSLKTSSFV PFAGNLITEL FLCSDNKYYV RHLVNQQVYP LTDCGYGPSG 

       430        440        450        460 
ASDGLCELSA YLNSSVRVNS TSNGIANFNS QCQAHSTNVT VYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The structural gene coding for thiamin-repressible acid phosphatase in Schizosaccharomyces pombe." Yang J., Schweingruber M.E. Curr. Genet. 18:269-272(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-30.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-71. Strain: ATCC 38364 / 968.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56939 Genomic DNA. Translation: CAA40258.1. CU329671 Genomic DNA. Translation: CAA22278.1. AB027777 Genomic DNA. Translation: BAA87081.1.
PIR	S14119.
RefSeq	NP_595181.1. NM_001021089.2.
3D structure databases
ProteinModelPortal	Q01682.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC428.03c-1.
Proteomic databases
PaxDb	Q01682.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC428.03c.1; SPBC428.03c.1:pep; SPBC428.03c.
GeneID	2540606.
KEGG	spo:SPBC428.03c.
Organism-specific databases
PomBase	SPBC428.03c.
Phylogenomic databases
eggNOG	NOG260296.
HOGENOM	HOG000115655.
KO	K01078.
OMA	DESNVEY.
OrthoDB	EOG45HW5S.
Family and domain databases
InterPro	IPR000560. His_Pase_superF_clade-2. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. His_Phos_2. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801731.

Entry information

Entry name

PPA2_SCHPO

Accession

Primary (citable) accession number: Q01682
Secondary accession number(s): Q9UU70

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents