Reviewed, UniProtKB/Swiss-Prot Q01682 (PPA2_SCHPO)

Names and origin

Protein names

Recommended name:
    Thiamine-repressible acid phosphatase pho4
    EC=3.1.3.2

Gene names

Name:	pho4
ORF Names:	SPBC428.03c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May dephosphorylate thiamine phosphates.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Secreted › cell wall.
Induction	Repressed by thiamine.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from direct assay. Source: GeneDB_SPombe cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe external side of plasma membrane Non-traceable author statement. Source: GeneDB_SPombe extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.1
	Chain	19 – 463	445	Thiamine-repressible acid phosphatase pho4		PRO_0000023952
Sites
	Active site	69	1	Nucleophile By similarity
	Active site	341	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
	Glycosylation	221	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	433	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01682-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: F48EAFF8BB6B234A	FASTA	463	52,119
	10 20 30 40 50 60 MKLSGISLWL LAASIVHAGK SQFEAFENEF YFKDHLGTIS VYHEPYFNGP TTSFPESCAI 70 80 90 100 110 120 KQVHLLQRHG SRNPTGDDTA TDVSSAQYID IFQNKLLNGS IPVNFSYPEN PLYFVKHWTP 130 140 150 160 170 180 VIKAENADQL SSSGRIELFD LGRQVFERYY ELFDTDVYDI NTAAQERVVD SAEWFSYGMF 190 200 210 220 230 240 GDDMQNKTNF IVLPEDDSAG ANSLAMYYSC PVYEDNNIDE NTTEAAHTSW RNVFLKPIAN 250 260 270 280 290 300 RLNKYFDSGY NLTVSDVRSL YYICVYEIAL RDNSDFCSLF TPSEFLNFEY DSDLDYAYWG 310 320 330 340 350 360 GPASEWASTL GGAYVNNLAN NLRKGVNNAS DRKVFLAFTH DSQIIPVEAA LGFFPDITPE 370 380 390 400 410 420 HPLPTDKNIF TYSLKTSSFV PFAGNLITEL FLCSDNKYYV RHLVNQQVYP LTDCGYGPSG 430 440 450 460 ASDGLCELSA YLNSSVRVNS TSNGIANFNS QCQAHSTNVT VYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The structural gene coding for thiamin-repressible acid phosphatase in Schizosaccharomyces pombe." Yang J., Schweingruber M.E. Curr. Genet. 18:269-272(1990) [PubMed: 2249257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-30.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-71. Strain: ATCC 38364 / 968.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56939 Genomic DNA. Translation: CAA40258.1. CU329671 Genomic DNA. Translation: CAA22278.1. AB027777 Genomic DNA. Translation: BAA87081.1.
PIR	S14119.
RefSeq	NP_595181.1.
3D structure databases
HSSP	HSSP built from PDB template 1QFX based on UniProtKB P34755.
ModBase	Search...
Protein-protein interaction databases
STRING	Q01682.
Genome annotation databases
GeneID	2540606.
GenomeReviews	Gene locus pho4 in contig CU329671_GR.
KEGG	spo:SPBC428.03c.
NMPDR	fig|4896.1.peg.1047.
Organism-specific databases
GeneDB_Spombe	SPBC428.03c.
Phylogenomic databases
OMA	AGSVQLN.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003273-MON.
BRENDA	3.1.3.2. 653.
Gene expression databases
ArrayExpress	Q01682.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA2_SCHPO

Accession

Primary (citable) accession number: Q01682
Secondary accession number(s): Q9UU70

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)