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Protein

Large neutral amino acids transporter small subunit 1

Gene

SLC7A5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.14 Publications

Kineticsi

  1. KM=7.9 µM for T4 (in the presence of choline chloride)5 Publications
  2. KM=0.8 µM for T3 (in the presence of choline chloride)5 Publications
  3. KM=12.5 µM for reverse triiodothyronine (rT3) (in the presence of choline chloride)5 Publications
  4. KM=7.9 µM for 3,3'-diiodothyronine (in the presence of choline chloride)5 Publications
  5. KM=46 µM for leucine (in the presence of choline chloride)5 Publications
  6. KM=19 µM for tryptophan (in the presence of choline chloride)5 Publications
  7. KM=32 µM for L-leucine5 Publications
  8. KM=10 mM for L-alanine5 Publications
  9. KM=2.2 mM for L-glutamine5 Publications
  10. KM=35 µM for L-histidine5 Publications
  11. KM=740 µM for L-phenylalanine5 Publications
  12. KM=98 µM for MeHg-L-cysteine5 Publications
  13. KM=99 µM for methionine5 Publications
  14. KM=55.2 µM for phenylalanine (in T24 human bladder carcinoma cells)5 Publications
  15. KM=60.4 µM for tyrosine (in T24 human bladder carcinoma cells)5 Publications
  16. KM=16.4 µM for tyrosine (in human fibroblasts)5 Publications
  17. KM=138 µM for Dopa (in T24 human bladder carcinoma cells)5 Publications
  18. KM=96.5 µM for 3-O-methyldopa (in T24 human bladder carcinoma cells)5 Publications
  19. KM=153 µM for alpha-methyltyrosine (in T24 human bladder carcinoma cells)5 Publications
  20. KM=216 µM for alpha-methyldopa (in T24 human bladder carcinoma cells)5 Publications
  21. KM=191 µM for gabapentin (in T24 human bladder carcinoma cells)5 Publications
  22. KM=7.3 µM for triiodothyronine (in T24 human bladder carcinoma cells)5 Publications
  23. KM=162 µM for thyroxine (in T24 human bladder carcinoma cells)5 Publications
  24. KM=75.3 µM for melphanan (in T24 human bladder carcinoma cells)5 Publications
  25. KM=156 µM for BCH (in T24 human bladder carcinoma cells)5 Publications

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Amino-acid transport, Differentiation, Neurogenesis, Transport

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000103257-MONOMER.
    ReactomeiREACT_12560. Basigin interactions.
    REACT_13796. Amino acid transport across the plasma membrane.
    SABIO-RKQ01650.

    Protein family/group databases

    TCDBi2.A.3.8.25. the amino acid-polyamine-organocation (apc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 1
    Alternative name(s):
    4F2 light chain
    Short name:
    4F2 LC
    Short name:
    4F2LC
    CD98 light chain
    Integral membrane protein E16
    L-type amino acid transporter 1
    Short name:
    hLAT1
    Solute carrier family 7 member 5
    y+ system cationic amino acid transporter
    Gene namesi
    Name:SLC7A5
    Synonyms:CD98LC, LAT1, MPE16
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:11063. SLC7A5.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei50 – 7021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei84 – 10421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei120 – 14021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei199 – 21921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei274 – 29421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 33921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei396 – 41621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei431 – 45121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei458 – 47821HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35923.

    Chemistry

    DrugBankiDB00509. Dextrothyroxine.
    DB01235. L-DOPA.
    DB00451. Levothyroxine.
    DB00279. Liothyronine.
    DB01042. Melphalan.

    Polymorphism and mutation databases

    BioMutaiSLC7A5.
    DMDMi12643412.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 507507Large neutral amino acids transporter small subunit 1PRO_0000054270Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki19 – 19Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
    Cross-linki30 – 30Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
    Modified residuei31 – 311Phosphoserine3 Publications
    Modified residuei45 – 451Phosphothreonine1 Publication
    Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ01650.
    PeptideAtlasiQ01650.
    PRIDEiQ01650.

    PTM databases

    PhosphoSiteiQ01650.

    Expressioni

    Tissue specificityi

    Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.9 Publications

    Inductioni

    Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore.2 Publications

    Gene expression databases

    BgeeiQ01650.
    CleanExiHS_SLC7A5.
    GenevisibleiQ01650. HS.

    Organism-specific databases

    HPAiHPA052673.
    HPA056077.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.8 Publications

    Protein-protein interaction databases

    BioGridi113801. 6 interactions.
    IntActiQ01650. 5 interactions.
    MINTiMINT-5000558.
    STRINGi9606.ENSP00000261622.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01650.
    SMRiQ01650. Positions 48-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ01650.
    KOiK13780.
    OMAiMIWLRHR.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ01650.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q01650-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAGAGPKRRA LAAPAAEEKE EAREKMLAAK SADGSAPAGE GEGVTLQRNI
    60 70 80 90 100
    TLLNGVAIIV GTIIGSGIFV TPTGVLKEAG SPGLALVVWA ACGVFSIVGA
    110 120 130 140 150
    LCYAELGTTI SKSGGDYAYM LEVYGSLPAF LKLWIELLII RPSSQYIVAL
    160 170 180 190 200
    VFATYLLKPL FPTCPVPEEA AKLVACLCVL LLTAVNCYSV KAATRVQDAF
    210 220 230 240 250
    AAAKLLALAL IILLGFVQIG KGDVSNLDPN FSFEGTKLDV GNIVLALYSG
    260 270 280 290 300
    LFAYGGWNYL NFVTEEMINP YRNLPLAIII SLPIVTLVYV LTNLAYFTTL
    310 320 330 340 350
    STEQMLSSEA VAVDFGNYHL GVMSWIIPVF VGLSCFGSVN GSLFTSSRLF
    360 370 380 390 400
    FVGSREGHLP SILSMIHPQL LTPVPSLVFT CVMTLLYAFS KDIFSVINFF
    410 420 430 440 450
    SFFNWLCVAL AIIGMIWLRH RKPELERPIK VNLALPVFFI LACLFLIAVS
    460 470 480 490 500
    FWKTPVECGI GFTIILSGLP VYFFGVWWKN KPKWLLQGIF STTVLCQKLM

    QVVPQET
    Length:507
    Mass (Da):55,010
    Last modified:January 24, 2001 - v2
    Checksum:i767F3C60B62C0F02
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151A → V in BAA75746 (Ref. 5) Curated
    Sequence conflicti29 – 313AKS → SKR in BAA75746 (Ref. 5) Curated
    Sequence conflicti35 – 351S → A in BAA75746 (Ref. 5) Curated
    Sequence conflicti62 – 621T → A in BAA75746 (Ref. 5) Curated
    Sequence conflicti88 – 881V → M in BAA75746 (Ref. 5) Curated
    Sequence conflicti154 – 1541T → A in BAA75746 (Ref. 5) Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti223 – 2231D → V.1 Publication
    Corresponds to variant rs17853937 [ dbSNP | Ensembl ].
    VAR_070119
    Natural varianti230 – 2301N → K.1 Publication
    Corresponds to variant rs1060250 [ dbSNP | Ensembl ].
    VAR_048157

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF077866 mRNA. Translation: AAC61479.1.
    AF104032 mRNA. Translation: AAD20464.1.
    AB018542 mRNA. Translation: BAA33851.1.
    AB018009 mRNA. Translation: BAA84648.1.
    AB017908 mRNA. Translation: BAA75746.1.
    BC039692 mRNA. Translation: AAH39692.1.
    BC042600 mRNA. Translation: AAH42600.1.
    M80244 mRNA. Translation: AAA35780.1.
    CCDSiCCDS10964.1.
    PIRiJG0165.
    RefSeqiNP_003477.4. NM_003486.5.
    UniGeneiHs.513797.

    Genome annotation databases

    EnsembliENST00000261622; ENSP00000261622; ENSG00000103257.
    GeneIDi8140.
    KEGGihsa:8140.
    UCSCiuc002fkm.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF077866 mRNA. Translation: AAC61479.1.
    AF104032 mRNA. Translation: AAD20464.1.
    AB018542 mRNA. Translation: BAA33851.1.
    AB018009 mRNA. Translation: BAA84648.1.
    AB017908 mRNA. Translation: BAA75746.1.
    BC039692 mRNA. Translation: AAH39692.1.
    BC042600 mRNA. Translation: AAH42600.1.
    M80244 mRNA. Translation: AAA35780.1.
    CCDSiCCDS10964.1.
    PIRiJG0165.
    RefSeqiNP_003477.4. NM_003486.5.
    UniGeneiHs.513797.

    3D structure databases

    ProteinModelPortaliQ01650.
    SMRiQ01650. Positions 48-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi113801. 6 interactions.
    IntActiQ01650. 5 interactions.
    MINTiMINT-5000558.
    STRINGi9606.ENSP00000261622.

    Chemistry

    ChEMBLiCHEMBL4459.
    DrugBankiDB00509. Dextrothyroxine.
    DB01235. L-DOPA.
    DB00451. Levothyroxine.
    DB00279. Liothyronine.
    DB01042. Melphalan.

    Protein family/group databases

    TCDBi2.A.3.8.25. the amino acid-polyamine-organocation (apc) family.

    PTM databases

    PhosphoSiteiQ01650.

    Polymorphism and mutation databases

    BioMutaiSLC7A5.
    DMDMi12643412.

    Proteomic databases

    MaxQBiQ01650.
    PeptideAtlasiQ01650.
    PRIDEiQ01650.

    Protocols and materials databases

    DNASUi8140.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000261622; ENSP00000261622; ENSG00000103257.
    GeneIDi8140.
    KEGGihsa:8140.
    UCSCiuc002fkm.3. human.

    Organism-specific databases

    CTDi8140.
    GeneCardsiGC16M087863.
    HGNCiHGNC:11063. SLC7A5.
    HPAiHPA052673.
    HPA056077.
    MIMi600182. gene.
    neXtProtiNX_Q01650.
    PharmGKBiPA35923.
    GenAtlasiSearch...

    Phylogenomic databases

    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ01650.
    KOiK13780.
    OMAiMIWLRHR.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ01650.
    TreeFamiTF313355.

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000103257-MONOMER.
    ReactomeiREACT_12560. Basigin interactions.
    REACT_13796. Amino acid transport across the plasma membrane.
    SABIO-RKQ01650.

    Miscellaneous databases

    ChiTaRSiSLC7A5. human.
    GeneWikiiSLC7A5.
    GenomeRNAii8140.
    NextBioi30811.
    PROiQ01650.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ01650.
    CleanExiHS_SLC7A5.
    GenevisibleiQ01650. HS.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family."
      Mastroberardino L., Spindler B., Pfeiffer R., Skelly P.J., Loffing J., Shoemaker C.B., Verrey F.
      Nature 395:288-291(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, VARIANT LYS-230.
    2. "Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function."
      Prasad P.D., Wang H., Huang W., Kekuda R., Rajan D.P., Leibach F.H., Ganapathy V.
      Biochem. Biophys. Res. Commun. 255:283-288(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
      Tissue: Placenta.
    3. "Primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16."
      Tsurudome M., Ito M., Takebayashi S., Okumura K., Nishio M., Kawano M., Kusagawa S., Komada H., Ito Y.
      J. Immunol. 162:2462-2466(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
    4. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
      Tissue: Ovary.
    5. "Human 4F2 light chain: amino acid transporter."
      Minato N., Iwai K., Takizawa C., Nakamura E.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-223.
      Tissue: Liver and Lymph.
    7. "A novel transiently expressed, integral membrane protein linked to cell activation. Molecular cloning via the rapid degradation signal AUUUA."
      Gaugitsch H.W., Prieschl E.E., Kalthoff F., Huber N.E., Baumruker T.
      J. Biol. Chem. 267:11267-11273(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-507, TISSUE SPECIFICITY, INDUCTION.
      Tissue: Peripheral blood lymphocyte.
    8. "Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids."
      Pineda M., Fernandez E., Torrents D., Estevez R., Lopez C., Camps M., Lloberas J., Zorzano A., Palacin M.
      J. Biol. Chem. 274:19738-19744(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INHIBITION.
    9. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
      Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
      J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    10. "Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains."
      Broeer A., Friedrich B., Wagner C.A., Fillon S., Ganapathy V., Lang F., Broeer S.
      Biochem. J. 355:725-731(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    11. "Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta."
      Ritchie J.W.A., Taylor P.M.
      Biochem. J. 356:719-725(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.
    12. "Thyroid hormone transport by the heterodimeric human system L amino acid transporter."
      Friesema E.C.H., Docter R., Moerings E.P.C.M., Verrey F., Krenning E.P., Hennemann G., Visser T.J.
      Endocrinology 142:4339-4348(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INHIBITION.
    13. "Expression and regulation of 4F2hc and hLAT1 in human trophoblasts."
      Okamoto Y., Sakata M., Ogura K., Yamamoto T., Yamaguchi M., Tasaka K., Kurachi H., Tsurudome M., Murata Y.
      Am. J. Physiol. 282:C196-C204(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    14. "Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2."
      Simmons-Willis T.A., Koh A.S., Clarkson T.W., Ballatori N.
      Biochem. J. 367:239-246(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
    15. "Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells."
      Kim D.K., Kanai Y., Choi H.W., Tangtrongsup S., Chairoungdua A., Babu E., Tachampa K., Anzai N., Iribe Y., Endou H.
      Biochim. Biophys. Acta 1565:112-121(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INHIBITION.
    16. "Identification and functional characterization of a Na+-independent large neutral amino acid transporter, LAT1, in human and rabbit cornea."
      Jain-Vakkalagadda B., Dey S., Pal D., Mitra A.K.
      Invest. Ophthalmol. Vis. Sci. 44:2919-2927(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    17. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
      Fraga S., Pinho M.J., Soares-da-Silva P.
      Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    18. "Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols."
      Li S., Whorton A.R.
      J. Biol. Chem. 280:20102-20110(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INHIBITION.
    19. "L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors."
      Nawashiro H., Otani N., Shinomiya N., Fukui S., Ooigawa H., Shima K., Matsuo H., Kanai Y., Endou H.
      Int. J. Cancer 119:484-492(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    20. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    21. "Functional characterization of tyrosine transport in fibroblast cells from healthy controls."
      Vumma R., Wiesel F.A., Flyckt L., Bjerkenstedt L., Venizelos N.
      Neurosci. Lett. 434:56-60(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
    22. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    23. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    24. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND THR-45, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    25. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLAT1_HUMAN
    AccessioniPrimary (citable) accession number: Q01650
    Secondary accession number(s): Q8IV97
    , Q9UBN8, Q9UP15, Q9UQC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 24, 2001
    Last modified: July 22, 2015
    This is version 158 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The uptake of leucine, tyrosine and tryptophan is inhibited by the different iodothyronines, in particular T3. Leucine transport is also inhibited by small zwitterionic amino acids (i.e. glycine, alanine, serine, threonine and cysteine) and by glutamine and asparginine. The uptake of T3 is almost completely blocked by coincubation with leucine, tryptophan, tyrosine, and phenylalanine, or 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH). Methionine uptake was inhibited by the L-system substrates L-leucine, BCH, L-cysteine and by the MeHg-L-cysteine complex and structurally related S-ethyl-L-cysteine. MeHg-L-cysteine uptake is inhibited by L-methionine, L-leucine, BCH and S-ethyl-L-cysteine. L-leucine uptake was inhibited by L-CNSO. Tyrosine uptake in fibroblasts was inhibited by D-methionine, and methyl-aminoisobutyric acid (MeAIB).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.