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Q01650

- LAT1_HUMAN

UniProt

Q01650 - LAT1_HUMAN

Protein

Large neutral amino acids transporter small subunit 1

Gene

SLC7A5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 2 (24 Jan 2001)
      Previous versions | rss
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    Functioni

    Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.14 Publications

    Kineticsi

    1. KM=7.9 µM for T4 (in the presence of choline chloride)5 Publications
    2. KM=0.8 µM for T3 (in the presence of choline chloride)5 Publications
    3. KM=12.5 µM for reverse triiodothyronine (rT3) (in the presence of choline chloride)5 Publications
    4. KM=7.9 µM for 3,3'-diiodothyronine (in the presence of choline chloride)5 Publications
    5. KM=46 µM for leucine (in the presence of choline chloride)5 Publications
    6. KM=19 µM for tryptophan (in the presence of choline chloride)5 Publications
    7. KM=32 µM for L-leucine5 Publications
    8. KM=10 mM for L-alanine5 Publications
    9. KM=2.2 mM for L-glutamine5 Publications
    10. KM=35 µM for L-histidine5 Publications
    11. KM=740 µM for L-phenylalanine5 Publications
    12. KM=98 µM for MeHg-L-cysteine5 Publications
    13. KM=99 µM for methionine5 Publications
    14. KM=55.2 µM for phenylalanine (in T24 human bladder carcinoma cells)5 Publications
    15. KM=60.4 µM for tyrosine (in T24 human bladder carcinoma cells)5 Publications
    16. KM=16.4 µM for tyrosine (in human fibroblasts)5 Publications
    17. KM=138 µM for Dopa (in T24 human bladder carcinoma cells)5 Publications
    18. KM=96.5 µM for 3-O-methyldopa (in T24 human bladder carcinoma cells)5 Publications
    19. KM=153 µM for alpha-methyltyrosine (in T24 human bladder carcinoma cells)5 Publications
    20. KM=216 µM for alpha-methyldopa (in T24 human bladder carcinoma cells)5 Publications
    21. KM=191 µM for gabapentin (in T24 human bladder carcinoma cells)5 Publications
    22. KM=7.3 µM for triiodothyronine (in T24 human bladder carcinoma cells)5 Publications
    23. KM=162 µM for thyroxine (in T24 human bladder carcinoma cells)5 Publications
    24. KM=75.3 µM for melphanan (in T24 human bladder carcinoma cells)5 Publications
    25. KM=156 µM for BCH (in T24 human bladder carcinoma cells)5 Publications

    GO - Molecular functioni

    1. amino acid transmembrane transporter activity Source: UniProtKB
    2. L-amino acid transmembrane transporter activity Source: Ensembl
    3. neutral amino acid transmembrane transporter activity Source: ProtInc
    4. peptide antigen binding Source: UniProtKB

    GO - Biological processi

    1. amino acid transport Source: Reactome
    2. blood coagulation Source: Reactome
    3. cell differentiation Source: UniProtKB-KW
    4. cellular amino acid metabolic process Source: ProtInc
    5. ion transport Source: Reactome
    6. leukocyte migration Source: Reactome
    7. nervous system development Source: UniProtKB-KW
    8. neutral amino acid transport Source: UniProtKB
    9. transmembrane transport Source: Reactome
    10. transport Source: ProtInc

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Amino-acid transport, Differentiation, Neurogenesis, Transport

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000103257-MONOMER.
    ReactomeiREACT_12560. Basigin interactions.
    REACT_13796. Amino acid transport across the plasma membrane.
    SABIO-RKQ01650.

    Protein family/group databases

    TCDBi2.A.3.8.25. the amino acid-polyamine-organocation (apc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 1
    Alternative name(s):
    4F2 light chain
    Short name:
    4F2 LC
    Short name:
    4F2LC
    CD98 light chain
    Integral membrane protein E16
    L-type amino acid transporter 1
    Short name:
    hLAT1
    Solute carrier family 7 member 5
    y+ system cationic amino acid transporter
    Gene namesi
    Name:SLC7A5
    Synonyms:CD98LC, LAT1, MPE16
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:11063. SLC7A5.

    Subcellular locationi

    Cytoplasmcytosol. Apical cell membrane; Multi-pass membrane protein
    Note: Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. cytosol Source: UniProtKB-SubCell
    3. extracellular vesicular exosome Source: UniProt
    4. integral component of membrane Source: UniProtKB-KW
    5. membrane Source: UniProtKB
    6. nucleolus Source: HPA
    7. nucleus Source: HPA
    8. plasma membrane Source: HPA

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35923.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 507507Large neutral amino acids transporter small subunit 1PRO_0000054270Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki19 – 19Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
    Cross-linki30 – 30Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
    Modified residuei31 – 311Phosphoserine3 Publications
    Modified residuei45 – 451Phosphothreonine1 Publication
    Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ01650.
    PeptideAtlasiQ01650.
    PRIDEiQ01650.

    PTM databases

    PhosphoSiteiQ01650.

    Expressioni

    Tissue specificityi

    Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.9 Publications

    Inductioni

    Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore.2 Publications

    Gene expression databases

    BgeeiQ01650.
    CleanExiHS_SLC7A5.
    GenevestigatoriQ01650.

    Organism-specific databases

    HPAiHPA052673.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.8 Publications

    Protein-protein interaction databases

    BioGridi113801. 6 interactions.
    IntActiQ01650. 3 interactions.
    MINTiMINT-5000558.
    STRINGi9606.ENSP00000261622.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01650.
    SMRiQ01650. Positions 48-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei50 – 7021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei84 – 10421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei120 – 14021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei199 – 21921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei274 – 29421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 33921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei396 – 41621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei431 – 45121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei458 – 47821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ01650.
    KOiK13780.
    OMAiVFTCIMT.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ01650.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q01650-1 [UniParc]FASTAAdd to Basket

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    MAGAGPKRRA LAAPAAEEKE EAREKMLAAK SADGSAPAGE GEGVTLQRNI    50
    TLLNGVAIIV GTIIGSGIFV TPTGVLKEAG SPGLALVVWA ACGVFSIVGA 100
    LCYAELGTTI SKSGGDYAYM LEVYGSLPAF LKLWIELLII RPSSQYIVAL 150
    VFATYLLKPL FPTCPVPEEA AKLVACLCVL LLTAVNCYSV KAATRVQDAF 200
    AAAKLLALAL IILLGFVQIG KGDVSNLDPN FSFEGTKLDV GNIVLALYSG 250
    LFAYGGWNYL NFVTEEMINP YRNLPLAIII SLPIVTLVYV LTNLAYFTTL 300
    STEQMLSSEA VAVDFGNYHL GVMSWIIPVF VGLSCFGSVN GSLFTSSRLF 350
    FVGSREGHLP SILSMIHPQL LTPVPSLVFT CVMTLLYAFS KDIFSVINFF 400
    SFFNWLCVAL AIIGMIWLRH RKPELERPIK VNLALPVFFI LACLFLIAVS 450
    FWKTPVECGI GFTIILSGLP VYFFGVWWKN KPKWLLQGIF STTVLCQKLM 500
    QVVPQET 507
    Length:507
    Mass (Da):55,010
    Last modified:January 24, 2001 - v2
    Checksum:i767F3C60B62C0F02
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151A → V in BAA75746. 1 PublicationCurated
    Sequence conflicti29 – 313AKS → SKR in BAA75746. 1 PublicationCurated
    Sequence conflicti35 – 351S → A in BAA75746. 1 PublicationCurated
    Sequence conflicti62 – 621T → A in BAA75746. 1 PublicationCurated
    Sequence conflicti88 – 881V → M in BAA75746. 1 PublicationCurated
    Sequence conflicti154 – 1541T → A in BAA75746. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti223 – 2231D → V.1 Publication
    Corresponds to variant rs17853937 [ dbSNP | Ensembl ].
    VAR_070119
    Natural varianti230 – 2301N → K.1 Publication
    Corresponds to variant rs1060250 [ dbSNP | Ensembl ].
    VAR_048157

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077866 mRNA. Translation: AAC61479.1.
    AF104032 mRNA. Translation: AAD20464.1.
    AB018542 mRNA. Translation: BAA33851.1.
    AB018009 mRNA. Translation: BAA84648.1.
    AB017908 mRNA. Translation: BAA75746.1.
    BC039692 mRNA. Translation: AAH39692.1.
    BC042600 mRNA. Translation: AAH42600.1.
    M80244 mRNA. Translation: AAA35780.1.
    CCDSiCCDS10964.1.
    PIRiJG0165.
    RefSeqiNP_003477.4. NM_003486.5.
    XP_006721350.1. XM_006721287.1.
    UniGeneiHs.513797.

    Genome annotation databases

    EnsembliENST00000261622; ENSP00000261622; ENSG00000103257.
    ENST00000565644; ENSP00000454323; ENSG00000103257.
    GeneIDi8140.
    KEGGihsa:8140.
    UCSCiuc002fkm.3. human.

    Polymorphism databases

    DMDMi12643412.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077866 mRNA. Translation: AAC61479.1 .
    AF104032 mRNA. Translation: AAD20464.1 .
    AB018542 mRNA. Translation: BAA33851.1 .
    AB018009 mRNA. Translation: BAA84648.1 .
    AB017908 mRNA. Translation: BAA75746.1 .
    BC039692 mRNA. Translation: AAH39692.1 .
    BC042600 mRNA. Translation: AAH42600.1 .
    M80244 mRNA. Translation: AAA35780.1 .
    CCDSi CCDS10964.1.
    PIRi JG0165.
    RefSeqi NP_003477.4. NM_003486.5.
    XP_006721350.1. XM_006721287.1.
    UniGenei Hs.513797.

    3D structure databases

    ProteinModelPortali Q01650.
    SMRi Q01650. Positions 48-414.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113801. 6 interactions.
    IntActi Q01650. 3 interactions.
    MINTi MINT-5000558.
    STRINGi 9606.ENSP00000261622.

    Chemistry

    BindingDBi Q01650.
    ChEMBLi CHEMBL4459.
    DrugBanki DB00509. Dextrothyroxine.
    DB01235. L-DOPA.
    DB00451. Levothyroxine.
    DB00279. Liothyronine.
    DB01042. Melphalan.

    Protein family/group databases

    TCDBi 2.A.3.8.25. the amino acid-polyamine-organocation (apc) family.

    PTM databases

    PhosphoSitei Q01650.

    Polymorphism databases

    DMDMi 12643412.

    Proteomic databases

    MaxQBi Q01650.
    PeptideAtlasi Q01650.
    PRIDEi Q01650.

    Protocols and materials databases

    DNASUi 8140.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261622 ; ENSP00000261622 ; ENSG00000103257 .
    ENST00000565644 ; ENSP00000454323 ; ENSG00000103257 .
    GeneIDi 8140.
    KEGGi hsa:8140.
    UCSCi uc002fkm.3. human.

    Organism-specific databases

    CTDi 8140.
    GeneCardsi GC16M087863.
    HGNCi HGNC:11063. SLC7A5.
    HPAi HPA052673.
    MIMi 600182. gene.
    neXtProti NX_Q01650.
    PharmGKBi PA35923.
    GenAtlasi Search...

    Phylogenomic databases

    HOGENOMi HOG000098892.
    HOVERGENi HBG000476.
    InParanoidi Q01650.
    KOi K13780.
    OMAi VFTCIMT.
    OrthoDBi EOG73BVCR.
    PhylomeDBi Q01650.
    TreeFami TF313355.

    Enzyme and pathway databases

    BioCyci MetaCyc:ENSG00000103257-MONOMER.
    Reactomei REACT_12560. Basigin interactions.
    REACT_13796. Amino acid transport across the plasma membrane.
    SABIO-RK Q01650.

    Miscellaneous databases

    ChiTaRSi SLC7A5. human.
    GeneWikii SLC7A5.
    GenomeRNAii 8140.
    NextBioi 30811.
    PROi Q01650.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q01650.
    CleanExi HS_SLC7A5.
    Genevestigatori Q01650.

    Family and domain databases

    InterProi IPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view ]
    PANTHERi PTHR11785. PTHR11785. 1 hit.
    Pfami PF13520. AA_permease_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsi TIGR00911. 2A0308. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family."
      Mastroberardino L., Spindler B., Pfeiffer R., Skelly P.J., Loffing J., Shoemaker C.B., Verrey F.
      Nature 395:288-291(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, VARIANT LYS-230.
    2. "Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function."
      Prasad P.D., Wang H., Huang W., Kekuda R., Rajan D.P., Leibach F.H., Ganapathy V.
      Biochem. Biophys. Res. Commun. 255:283-288(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
      Tissue: Placenta.
    3. "Primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16."
      Tsurudome M., Ito M., Takebayashi S., Okumura K., Nishio M., Kawano M., Kusagawa S., Komada H., Ito Y.
      J. Immunol. 162:2462-2466(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
    4. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
      Tissue: Ovary.
    5. "Human 4F2 light chain: amino acid transporter."
      Minato N., Iwai K., Takizawa C., Nakamura E.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-223.
      Tissue: Liver and Lymph.
    7. "A novel transiently expressed, integral membrane protein linked to cell activation. Molecular cloning via the rapid degradation signal AUUUA."
      Gaugitsch H.W., Prieschl E.E., Kalthoff F., Huber N.E., Baumruker T.
      J. Biol. Chem. 267:11267-11273(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-507, TISSUE SPECIFICITY, INDUCTION.
      Tissue: Peripheral blood lymphocyte.
    8. "Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids."
      Pineda M., Fernandez E., Torrents D., Estevez R., Lopez C., Camps M., Lloberas J., Zorzano A., Palacin M.
      J. Biol. Chem. 274:19738-19744(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INHIBITION.
    9. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
      Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
      J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    10. "Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains."
      Broeer A., Friedrich B., Wagner C.A., Fillon S., Ganapathy V., Lang F., Broeer S.
      Biochem. J. 355:725-731(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    11. "Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta."
      Ritchie J.W.A., Taylor P.M.
      Biochem. J. 356:719-725(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.
    12. "Thyroid hormone transport by the heterodimeric human system L amino acid transporter."
      Friesema E.C.H., Docter R., Moerings E.P.C.M., Verrey F., Krenning E.P., Hennemann G., Visser T.J.
      Endocrinology 142:4339-4348(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INHIBITION.
    13. "Expression and regulation of 4F2hc and hLAT1 in human trophoblasts."
      Okamoto Y., Sakata M., Ogura K., Yamamoto T., Yamaguchi M., Tasaka K., Kurachi H., Tsurudome M., Murata Y.
      Am. J. Physiol. 282:C196-C204(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    14. "Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2."
      Simmons-Willis T.A., Koh A.S., Clarkson T.W., Ballatori N.
      Biochem. J. 367:239-246(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
    15. "Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells."
      Kim D.K., Kanai Y., Choi H.W., Tangtrongsup S., Chairoungdua A., Babu E., Tachampa K., Anzai N., Iribe Y., Endou H.
      Biochim. Biophys. Acta 1565:112-121(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INHIBITION.
    16. "Identification and functional characterization of a Na+-independent large neutral amino acid transporter, LAT1, in human and rabbit cornea."
      Jain-Vakkalagadda B., Dey S., Pal D., Mitra A.K.
      Invest. Ophthalmol. Vis. Sci. 44:2919-2927(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    17. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
      Fraga S., Pinho M.J., Soares-da-Silva P.
      Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    18. "Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols."
      Li S., Whorton A.R.
      J. Biol. Chem. 280:20102-20110(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INHIBITION.
    19. "L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors."
      Nawashiro H., Otani N., Shinomiya N., Fukui S., Ooigawa H., Shima K., Matsuo H., Kanai Y., Endou H.
      Int. J. Cancer 119:484-492(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    20. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    21. "Functional characterization of tyrosine transport in fibroblast cells from healthy controls."
      Vumma R., Wiesel F.A., Flyckt L., Bjerkenstedt L., Venizelos N.
      Neurosci. Lett. 434:56-60(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
    22. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    23. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    24. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND THR-45, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    25. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    27. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLAT1_HUMAN
    AccessioniPrimary (citable) accession number: Q01650
    Secondary accession number(s): Q8IV97
    , Q9UBN8, Q9UP15, Q9UQC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 24, 2001
    Last modified: October 1, 2014
    This is version 149 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The uptake of leucine, tyrosine and tryptophan is inhibited by the different iodothyronines, in particular T3. Leucine transport is also inhibited by small zwitterionic amino acids (i.e. glycine, alanine, serine, threonine and cysteine) and by glutamine and asparginine. The uptake of T3 is almost completely blocked by coincubation with leucine, tryptophan, tyrosine, and phenylalanine, or 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH). Methionine uptake was inhibited by the L-system substrates L-leucine, BCH, L-cysteine and by the MeHg-L-cysteine complex and structurally related S-ethyl-L-cysteine. MeHg-L-cysteine uptake is inhibited by L-methionine, L-leucine, BCH and S-ethyl-L-cysteine. L-leucine uptake was inhibited by L-CNSO. Tyrosine uptake in fibroblasts was inhibited by D-methionine, and methyl-aminoisobutyric acid (MeAIB).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3