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Q01650

- LAT1_HUMAN

UniProt

Q01650 - LAT1_HUMAN

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Protein

Large neutral amino acids transporter small subunit 1

Gene

SLC7A5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.14 Publications

Kineticsi

  1. KM=7.9 µM for T4 (in the presence of choline chloride)5 Publications
  2. KM=0.8 µM for T3 (in the presence of choline chloride)5 Publications
  3. KM=12.5 µM for reverse triiodothyronine (rT3) (in the presence of choline chloride)5 Publications
  4. KM=7.9 µM for 3,3'-diiodothyronine (in the presence of choline chloride)5 Publications
  5. KM=46 µM for leucine (in the presence of choline chloride)5 Publications
  6. KM=19 µM for tryptophan (in the presence of choline chloride)5 Publications
  7. KM=32 µM for L-leucine5 Publications
  8. KM=10 mM for L-alanine5 Publications
  9. KM=2.2 mM for L-glutamine5 Publications
  10. KM=35 µM for L-histidine5 Publications
  11. KM=740 µM for L-phenylalanine5 Publications
  12. KM=98 µM for MeHg-L-cysteine5 Publications
  13. KM=99 µM for methionine5 Publications
  14. KM=55.2 µM for phenylalanine (in T24 human bladder carcinoma cells)5 Publications
  15. KM=60.4 µM for tyrosine (in T24 human bladder carcinoma cells)5 Publications
  16. KM=16.4 µM for tyrosine (in human fibroblasts)5 Publications
  17. KM=138 µM for Dopa (in T24 human bladder carcinoma cells)5 Publications
  18. KM=96.5 µM for 3-O-methyldopa (in T24 human bladder carcinoma cells)5 Publications
  19. KM=153 µM for alpha-methyltyrosine (in T24 human bladder carcinoma cells)5 Publications
  20. KM=216 µM for alpha-methyldopa (in T24 human bladder carcinoma cells)5 Publications
  21. KM=191 µM for gabapentin (in T24 human bladder carcinoma cells)5 Publications
  22. KM=7.3 µM for triiodothyronine (in T24 human bladder carcinoma cells)5 Publications
  23. KM=162 µM for thyroxine (in T24 human bladder carcinoma cells)5 Publications
  24. KM=75.3 µM for melphanan (in T24 human bladder carcinoma cells)5 Publications
  25. KM=156 µM for BCH (in T24 human bladder carcinoma cells)5 Publications

GO - Molecular functioni

  1. amino acid transmembrane transporter activity Source: UniProtKB
  2. L-amino acid transmembrane transporter activity Source: Ensembl
  3. neutral amino acid transmembrane transporter activity Source: ProtInc
  4. peptide antigen binding Source: UniProtKB

GO - Biological processi

  1. amino acid transport Source: Reactome
  2. blood coagulation Source: Reactome
  3. cell differentiation Source: UniProtKB-KW
  4. cellular amino acid metabolic process Source: ProtInc
  5. ion transport Source: Reactome
  6. leukocyte migration Source: Reactome
  7. nervous system development Source: UniProtKB-KW
  8. neutral amino acid transport Source: UniProtKB
  9. transmembrane transport Source: Reactome
  10. transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Amino-acid transport, Differentiation, Neurogenesis, Transport

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000103257-MONOMER.
ReactomeiREACT_12560. Basigin interactions.
REACT_13796. Amino acid transport across the plasma membrane.
SABIO-RKQ01650.

Protein family/group databases

TCDBi2.A.3.8.25. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Large neutral amino acids transporter small subunit 1
Alternative name(s):
4F2 light chain
Short name:
4F2 LC
Short name:
4F2LC
CD98 light chain
Integral membrane protein E16
L-type amino acid transporter 1
Short name:
hLAT1
Solute carrier family 7 member 5
y+ system cationic amino acid transporter
Gene namesi
Name:SLC7A5
Synonyms:CD98LC, LAT1, MPE16
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:11063. SLC7A5.

Subcellular locationi

Cytoplasmcytosol. Apical cell membrane; Multi-pass membrane protein
Note: Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: UniProt
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: UniProtKB
  5. nucleolus Source: HPA
  6. nucleus Source: HPA
  7. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35923.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Large neutral amino acids transporter small subunit 1PRO_0000054270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki19 – 19Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-linki30 – 30Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residuei31 – 311Phosphoserine3 Publications
Modified residuei45 – 451Phosphothreonine1 Publication
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ01650.
PeptideAtlasiQ01650.
PRIDEiQ01650.

PTM databases

PhosphoSiteiQ01650.

Expressioni

Tissue specificityi

Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.9 Publications

Inductioni

Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore.2 Publications

Gene expression databases

BgeeiQ01650.
CleanExiHS_SLC7A5.
GenevestigatoriQ01650.

Organism-specific databases

HPAiHPA052673.

Interactioni

Subunit structurei

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.8 Publications

Protein-protein interaction databases

BioGridi113801. 7 interactions.
IntActiQ01650. 3 interactions.
MINTiMINT-5000558.
STRINGi9606.ENSP00000261622.

Structurei

3D structure databases

ProteinModelPortaliQ01650.
SMRiQ01650. Positions 48-414.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei50 – 7021HelicalSequence AnalysisAdd
BLAST
Transmembranei84 – 10421HelicalSequence AnalysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence AnalysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence AnalysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence AnalysisAdd
BLAST
Transmembranei274 – 29421HelicalSequence AnalysisAdd
BLAST
Transmembranei319 – 33921HelicalSequence AnalysisAdd
BLAST
Transmembranei396 – 41621HelicalSequence AnalysisAdd
BLAST
Transmembranei431 – 45121HelicalSequence AnalysisAdd
BLAST
Transmembranei458 – 47821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119037.
HOGENOMiHOG000098892.
HOVERGENiHBG000476.
InParanoidiQ01650.
KOiK13780.
OMAiVFTCIMT.
OrthoDBiEOG73BVCR.
PhylomeDBiQ01650.
TreeFamiTF313355.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00911. 2A0308. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01650-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGAGPKRRA LAAPAAEEKE EAREKMLAAK SADGSAPAGE GEGVTLQRNI
60 70 80 90 100
TLLNGVAIIV GTIIGSGIFV TPTGVLKEAG SPGLALVVWA ACGVFSIVGA
110 120 130 140 150
LCYAELGTTI SKSGGDYAYM LEVYGSLPAF LKLWIELLII RPSSQYIVAL
160 170 180 190 200
VFATYLLKPL FPTCPVPEEA AKLVACLCVL LLTAVNCYSV KAATRVQDAF
210 220 230 240 250
AAAKLLALAL IILLGFVQIG KGDVSNLDPN FSFEGTKLDV GNIVLALYSG
260 270 280 290 300
LFAYGGWNYL NFVTEEMINP YRNLPLAIII SLPIVTLVYV LTNLAYFTTL
310 320 330 340 350
STEQMLSSEA VAVDFGNYHL GVMSWIIPVF VGLSCFGSVN GSLFTSSRLF
360 370 380 390 400
FVGSREGHLP SILSMIHPQL LTPVPSLVFT CVMTLLYAFS KDIFSVINFF
410 420 430 440 450
SFFNWLCVAL AIIGMIWLRH RKPELERPIK VNLALPVFFI LACLFLIAVS
460 470 480 490 500
FWKTPVECGI GFTIILSGLP VYFFGVWWKN KPKWLLQGIF STTVLCQKLM

QVVPQET
Length:507
Mass (Da):55,010
Last modified:January 24, 2001 - v2
Checksum:i767F3C60B62C0F02
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151A → V in BAA75746. 1 PublicationCurated
Sequence conflicti29 – 313AKS → SKR in BAA75746. 1 PublicationCurated
Sequence conflicti35 – 351S → A in BAA75746. 1 PublicationCurated
Sequence conflicti62 – 621T → A in BAA75746. 1 PublicationCurated
Sequence conflicti88 – 881V → M in BAA75746. 1 PublicationCurated
Sequence conflicti154 – 1541T → A in BAA75746. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti223 – 2231D → V.1 Publication
Corresponds to variant rs17853937 [ dbSNP | Ensembl ].
VAR_070119
Natural varianti230 – 2301N → K.1 Publication
Corresponds to variant rs1060250 [ dbSNP | Ensembl ].
VAR_048157

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077866 mRNA. Translation: AAC61479.1.
AF104032 mRNA. Translation: AAD20464.1.
AB018542 mRNA. Translation: BAA33851.1.
AB018009 mRNA. Translation: BAA84648.1.
AB017908 mRNA. Translation: BAA75746.1.
BC039692 mRNA. Translation: AAH39692.1.
BC042600 mRNA. Translation: AAH42600.1.
M80244 mRNA. Translation: AAA35780.1.
CCDSiCCDS10964.1.
PIRiJG0165.
RefSeqiNP_003477.4. NM_003486.5.
XP_006721350.1. XM_006721287.1.
UniGeneiHs.513797.

Genome annotation databases

EnsembliENST00000261622; ENSP00000261622; ENSG00000103257.
ENST00000565644; ENSP00000454323; ENSG00000103257.
GeneIDi8140.
KEGGihsa:8140.
UCSCiuc002fkm.3. human.

Polymorphism databases

DMDMi12643412.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077866 mRNA. Translation: AAC61479.1 .
AF104032 mRNA. Translation: AAD20464.1 .
AB018542 mRNA. Translation: BAA33851.1 .
AB018009 mRNA. Translation: BAA84648.1 .
AB017908 mRNA. Translation: BAA75746.1 .
BC039692 mRNA. Translation: AAH39692.1 .
BC042600 mRNA. Translation: AAH42600.1 .
M80244 mRNA. Translation: AAA35780.1 .
CCDSi CCDS10964.1.
PIRi JG0165.
RefSeqi NP_003477.4. NM_003486.5.
XP_006721350.1. XM_006721287.1.
UniGenei Hs.513797.

3D structure databases

ProteinModelPortali Q01650.
SMRi Q01650. Positions 48-414.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113801. 7 interactions.
IntActi Q01650. 3 interactions.
MINTi MINT-5000558.
STRINGi 9606.ENSP00000261622.

Chemistry

BindingDBi Q01650.
ChEMBLi CHEMBL4459.
DrugBanki DB00509. Dextrothyroxine.
DB01235. L-DOPA.
DB00451. Levothyroxine.
DB00279. Liothyronine.
DB01042. Melphalan.

Protein family/group databases

TCDBi 2.A.3.8.25. the amino acid-polyamine-organocation (apc) family.

PTM databases

PhosphoSitei Q01650.

Polymorphism databases

DMDMi 12643412.

Proteomic databases

MaxQBi Q01650.
PeptideAtlasi Q01650.
PRIDEi Q01650.

Protocols and materials databases

DNASUi 8140.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261622 ; ENSP00000261622 ; ENSG00000103257 .
ENST00000565644 ; ENSP00000454323 ; ENSG00000103257 .
GeneIDi 8140.
KEGGi hsa:8140.
UCSCi uc002fkm.3. human.

Organism-specific databases

CTDi 8140.
GeneCardsi GC16M087863.
HGNCi HGNC:11063. SLC7A5.
HPAi HPA052673.
MIMi 600182. gene.
neXtProti NX_Q01650.
PharmGKBi PA35923.
GenAtlasi Search...

Phylogenomic databases

GeneTreei ENSGT00760000119037.
HOGENOMi HOG000098892.
HOVERGENi HBG000476.
InParanoidi Q01650.
KOi K13780.
OMAi VFTCIMT.
OrthoDBi EOG73BVCR.
PhylomeDBi Q01650.
TreeFami TF313355.

Enzyme and pathway databases

BioCyci MetaCyc:ENSG00000103257-MONOMER.
Reactomei REACT_12560. Basigin interactions.
REACT_13796. Amino acid transport across the plasma membrane.
SABIO-RK Q01650.

Miscellaneous databases

ChiTaRSi SLC7A5. human.
GeneWikii SLC7A5.
GenomeRNAii 8140.
NextBioi 30811.
PROi Q01650.
SOURCEi Search...

Gene expression databases

Bgeei Q01650.
CleanExi HS_SLC7A5.
Genevestigatori Q01650.

Family and domain databases

InterProi IPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view ]
PANTHERi PTHR11785. PTHR11785. 1 hit.
Pfami PF13520. AA_permease_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006060. AA_transporter. 1 hit.
TIGRFAMsi TIGR00911. 2A0308. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family."
    Mastroberardino L., Spindler B., Pfeiffer R., Skelly P.J., Loffing J., Shoemaker C.B., Verrey F.
    Nature 395:288-291(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, VARIANT LYS-230.
  2. "Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function."
    Prasad P.D., Wang H., Huang W., Kekuda R., Rajan D.P., Leibach F.H., Ganapathy V.
    Biochem. Biophys. Res. Commun. 255:283-288(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
    Tissue: Placenta.
  3. "Primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16."
    Tsurudome M., Ito M., Takebayashi S., Okumura K., Nishio M., Kawano M., Kusagawa S., Komada H., Ito Y.
    J. Immunol. 162:2462-2466(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
  4. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
    Tissue: Ovary.
  5. "Human 4F2 light chain: amino acid transporter."
    Minato N., Iwai K., Takizawa C., Nakamura E.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-223.
    Tissue: Liver and Lymph.
  7. "A novel transiently expressed, integral membrane protein linked to cell activation. Molecular cloning via the rapid degradation signal AUUUA."
    Gaugitsch H.W., Prieschl E.E., Kalthoff F., Huber N.E., Baumruker T.
    J. Biol. Chem. 267:11267-11273(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-507, TISSUE SPECIFICITY, INDUCTION.
    Tissue: Peripheral blood lymphocyte.
  8. "Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids."
    Pineda M., Fernandez E., Torrents D., Estevez R., Lopez C., Camps M., Lloberas J., Zorzano A., Palacin M.
    J. Biol. Chem. 274:19738-19744(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INHIBITION.
  9. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
    Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
    J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  10. "Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains."
    Broeer A., Friedrich B., Wagner C.A., Fillon S., Ganapathy V., Lang F., Broeer S.
    Biochem. J. 355:725-731(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  11. "Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta."
    Ritchie J.W.A., Taylor P.M.
    Biochem. J. 356:719-725(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.
  12. "Thyroid hormone transport by the heterodimeric human system L amino acid transporter."
    Friesema E.C.H., Docter R., Moerings E.P.C.M., Verrey F., Krenning E.P., Hennemann G., Visser T.J.
    Endocrinology 142:4339-4348(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INHIBITION.
  13. "Expression and regulation of 4F2hc and hLAT1 in human trophoblasts."
    Okamoto Y., Sakata M., Ogura K., Yamamoto T., Yamaguchi M., Tasaka K., Kurachi H., Tsurudome M., Murata Y.
    Am. J. Physiol. 282:C196-C204(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  14. "Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2."
    Simmons-Willis T.A., Koh A.S., Clarkson T.W., Ballatori N.
    Biochem. J. 367:239-246(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
  15. "Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells."
    Kim D.K., Kanai Y., Choi H.W., Tangtrongsup S., Chairoungdua A., Babu E., Tachampa K., Anzai N., Iribe Y., Endou H.
    Biochim. Biophys. Acta 1565:112-121(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INHIBITION.
  16. "Identification and functional characterization of a Na+-independent large neutral amino acid transporter, LAT1, in human and rabbit cornea."
    Jain-Vakkalagadda B., Dey S., Pal D., Mitra A.K.
    Invest. Ophthalmol. Vis. Sci. 44:2919-2927(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  17. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
    Fraga S., Pinho M.J., Soares-da-Silva P.
    Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  18. "Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols."
    Li S., Whorton A.R.
    J. Biol. Chem. 280:20102-20110(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INHIBITION.
  19. "L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors."
    Nawashiro H., Otani N., Shinomiya N., Fukui S., Ooigawa H., Shima K., Matsuo H., Kanai Y., Endou H.
    Int. J. Cancer 119:484-492(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  20. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  21. "Functional characterization of tyrosine transport in fibroblast cells from healthy controls."
    Vumma R., Wiesel F.A., Flyckt L., Bjerkenstedt L., Venizelos N.
    Neurosci. Lett. 434:56-60(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
  22. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  23. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  24. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND THR-45, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  25. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  27. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLAT1_HUMAN
AccessioniPrimary (citable) accession number: Q01650
Secondary accession number(s): Q8IV97
, Q9UBN8, Q9UP15, Q9UQC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 24, 2001
Last modified: October 29, 2014
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The uptake of leucine, tyrosine and tryptophan is inhibited by the different iodothyronines, in particular T3. Leucine transport is also inhibited by small zwitterionic amino acids (i.e. glycine, alanine, serine, threonine and cysteine) and by glutamine and asparginine. The uptake of T3 is almost completely blocked by coincubation with leucine, tryptophan, tyrosine, and phenylalanine, or 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH). Methionine uptake was inhibited by the L-system substrates L-leucine, BCH, L-cysteine and by the MeHg-L-cysteine complex and structurally related S-ethyl-L-cysteine. MeHg-L-cysteine uptake is inhibited by L-methionine, L-leucine, BCH and S-ethyl-L-cysteine. L-leucine uptake was inhibited by L-CNSO. Tyrosine uptake in fibroblasts was inhibited by D-methionine, and methyl-aminoisobutyric acid (MeAIB).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

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