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Protein

Uridine 5'-monophosphate synthase

Gene

r-l

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.
Orotidine 5'-phosphate = UMP + CO2.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes UMP from orotate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Uridine 5'-monophosphate synthase (r-l)
  2. Uridine 5'-monophosphate synthase (r-l)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei320By similarity1

GO - Molecular functioni

  • orotate phosphoribosyltransferase activity Source: FlyBase
  • orotidine-5'-phosphate decarboxylase activity Source: FlyBase

GO - Biological processi

  • 'de novo' pyrimidine nucleobase biosynthetic process Source: FlyBase
  • 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionDecarboxylase, Glycosyltransferase, Lyase, Multifunctional enzyme, Transferase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

ReactomeiR-DME-500753 Pyrimidine biosynthesis
UniPathwayiUPA00070; UER00119
UPA00070; UER00120

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine 5'-monophosphate synthase
Short name:
UMP synthase
Alternative name(s):
Rudimentary-like protein
Including the following 2 domains:
Orotate phosphoribosyltransferase (EC:2.4.2.10)
Short name:
OPRTase
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMPdecase
Gene namesi
Name:r-l
ORF Names:CG3593
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003257 r-l

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001396521 – 493Uridine 5'-monophosphate synthaseAdd BLAST493

Proteomic databases

PaxDbiQ01637
PRIDEiQ01637

Expressioni

Gene expression databases

BgeeiFBgn0003257
GenevisibleiQ01637 DM

Interactioni

Protein-protein interaction databases

BioGridi6746417 interactors.
IntActiQ01637 1 interactor.
STRINGi7227.FBpp0083440

Structurei

3D structure databases

ProteinModelPortaliQ01637
SMRiQ01637
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 207OPRTaseAdd BLAST207
Regioni208 – 233Domain linkerAdd BLAST26
Regioni234 – 493OMPdecaseAdd BLAST260

Sequence similaritiesi

In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
In the C-terminal section; belongs to the OMP decarboxylase family.Curated

Phylogenomic databases

eggNOGiKOG1377 Eukaryota
COG0284 LUCA
COG0461 LUCA
GeneTreeiENSGT00390000001856
InParanoidiQ01637
KOiK13421
OMAiRVSMKPE
OrthoDBiEOG091G06JT
PhylomeDBiQ01637

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
Gene3Di3.20.20.701 hit
HAMAPiMF_01208 PyrE, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR014732 OMPdecase
IPR018089 OMPdecase_AS
IPR001754 OMPdeCOase_dom
IPR023031 OPRT
IPR004467 Or_phspho_trans_dom
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR011060 RibuloseP-bd_barrel
PfamiView protein in Pfam
PF00215 OMPdecase, 1 hit
PF00156 Pribosyltran, 1 hit
SMARTiView protein in SMART
SM00934 OMPdecase, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
SSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR00336 pyrE, 1 hit
TIGR01740 pyrF, 1 hit
PROSITEiView protein in PROSITE
PS00156 OMPDECASE, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Q01637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAQNSDKMR ALALKLFEIN AFKFGDFKMK VGINSPVYFD LRVIVSYPDV
60 70 80 90 100
MQTVSDLLVE HIKDKQLSAK HVCGVPYTAL PLATIVSVQQ GTPMLVRRKE
110 120 130 140 150
AKAYGTKKLV EGIFNAGDTC LIVEDVVTSG SSILDTVRDL QGEGIVVTDA
160 170 180 190 200
VVVVDREQGG VANIAKHGVR MHSLFTLSFL LNTLHEAGRI EKSTVEAVAK
210 220 230 240 250
YIAAVQINSD GTFVGGDKGD VVRANDLQRT KLTYENRANL AKSAVAKRLF
260 270 280 290 300
NLIASKQTNL CLAADLTHAD EILDVADKCG PYICLLKTHV DIVEDFSDKF
310 320 330 340 350
IADLQALAQR HNFLLMEDRK FADIGNTVSL QYGKGIYKIS SWADLVTAHT
360 370 380 390 400
LPGRSILQGL KAGLGEGGAG KERGVFLLAE MSASGNLIDA KYKENSNKIA
410 420 430 440 450
TEGADVDFVA GVVCQSSDAF AFPGLLQLTP GVKIDEGVDQ LGQQYQSPEH
460 470 480 490
VVKERGADIG VVGRGILKAS SPKQAAQTYR DRLWAAYQDR VAK
Length:493
Mass (Da):53,419
Last modified:September 13, 2005 - v2
Checksum:i5293C47D353E45B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47 – 51YPDVM → LGLPQ in AAA29012 (PubMed:8444350).Curated5
Sequence conflicti82L → R in AAA29012 (PubMed:8444350).Curated1
Sequence conflicti219 – 223GDVVR → VTFPA in AAA29012 (PubMed:8444350).Curated5
Sequence conflicti236N → S in AAA29012 (PubMed:8444350).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00968 Genomic DNA Translation: AAA29012.1
AE014297 Genomic DNA Translation: AAF55842.1
AY058714 mRNA Translation: AAL13943.1
X54230 mRNA Translation: CAA38138.1
PIRiJU0141
RefSeqiNP_524427.1, NM_079703.3
UniGeneiDm.1654

Genome annotation databases

EnsemblMetazoaiFBtr0084038; FBpp0083440; FBgn0003257
GeneIDi42493
KEGGidme:Dmel_CG3593

Similar proteinsi

Entry informationi

Entry nameiUMPS_DROME
AccessioniPrimary (citable) accession number: Q01637
Secondary accession number(s): Q24221, Q9VDF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 13, 2005
Last modified: February 28, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome