Reviewed, UniProtKB/Swiss-Prot Q01637 (PYR5_DROME)

Names and origin

Protein names

Recommended name:
    Uridine 5'-monophosphate synthase
      Short name=UMP synthase
Alternative name(s):
    Rudimentary-like protein
Including the following 2 domains:
    1- Recommended name:
            Orotate phosphoribosyltransferase
                Short name=OPRTase
              EC=2.4.2.10
    2- Recommended name:
            Orotidine 5'-phosphate decarboxylase
              EC=4.1.1.23
        Alternative name(s):
            OMPdecase

Gene names

Name:	r-l
ORF Names:	CG3593

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Uridine 5'-monophosphate synthase		PRO_0000139652
Regions
	Region	1 – 207	207	OPRTase
	Region	208 – 233	26	Domain linker
	Region	234 – 493	260	OMPdecase
Sites
	Active site	320	1	By similarity
Experimental info
	Sequence conflict	47 – 51	5	YPDVM → LGLPQ in AAA29012. Ref.1
	Sequence conflict	82	1	L → R in AAA29012. Ref.1
	Sequence conflict	219 – 223	5	GDVVR → VTFPA in AAA29012. Ref.1
	Sequence conflict	236	1	N → S in AAA29012. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01637-1 [UniParc]. Last modified September 13, 2005. Version 2. Checksum: 5293C47D353E45B1	FASTA	493	53,419
	10 20 30 40 50 60 MVAQNSDKMR ALALKLFEIN AFKFGDFKMK VGINSPVYFD LRVIVSYPDV MQTVSDLLVE 70 80 90 100 110 120 HIKDKQLSAK HVCGVPYTAL PLATIVSVQQ GTPMLVRRKE AKAYGTKKLV EGIFNAGDTC 130 140 150 160 170 180 LIVEDVVTSG SSILDTVRDL QGEGIVVTDA VVVVDREQGG VANIAKHGVR MHSLFTLSFL 190 200 210 220 230 240 LNTLHEAGRI EKSTVEAVAK YIAAVQINSD GTFVGGDKGD VVRANDLQRT KLTYENRANL 250 260 270 280 290 300 AKSAVAKRLF NLIASKQTNL CLAADLTHAD EILDVADKCG PYICLLKTHV DIVEDFSDKF 310 320 330 340 350 360 IADLQALAQR HNFLLMEDRK FADIGNTVSL QYGKGIYKIS SWADLVTAHT LPGRSILQGL 370 380 390 400 410 420 KAGLGEGGAG KERGVFLLAE MSASGNLIDA KYKENSNKIA TEGADVDFVA GVVCQSSDAF 430 440 450 460 470 480 AFPGLLQLTP GVKIDEGVDQ LGQQYQSPEH VVKERGADIG VVGRGILKAS SPKQAAQTYR 490 DRLWAAYQDR VAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the rudimentary-like gene and UMP synthase in Drosophila melanogaster." Eisenberg M., Kirkpatrick R., Rawls J. Gene 124:263-267(1993) [PubMed: 8444350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Molecular cloning of the UMP synthase gene rudimentary-like from Drosophila melanogaster." Eisenberg M.T., Gathy K., Vincent T., Rawls J. Mol. Gen. Genet. 222:1-8(1990) [PubMed: 2122228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-78.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L00968 Genomic DNA. Translation: AAA29012.1. AE014297 Genomic DNA. Translation: AAF55842.1. AY058714 mRNA. Translation: AAL13943.1. X54230 mRNA. Translation: CAA38138.1.
PIR	JU0141.
RefSeq	NP_524427.1.
UniGene	Dm.1654
3D structure databases
HSSP	HSSP built from PDB template 1DQW based on UniProtKB P03962.
ModBase	Search...
Protein-protein interaction databases
STRING	Q01637.
Genome annotation databases
Ensembl	FBtr0084038; FBpp0083440; FBgn0003257; Drosophila melanogaster. [Genome view]
GeneID	42493.
KEGG	dme:Dmel_CG3593.
NMPDR	fig|7227.3.peg.13843.
Organism-specific databases
CTD	42493.
FlyBase	FBgn0003257. r-l.
Phylogenomic databases
HOGENOM	Q01637.
OMA	PLATVIC.
Enzyme and pathway databases
BRENDA	2.4.2.10. 48. 4.1.1.23. 48.
Gene expression databases
ArrayExpress	Q01637.
GermOnline	CG3593. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase_1_core. IPR018089. OMPdecase_AS. IPR001754. OMPdecase_core. IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit. TIGR01740. pyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	829081.

Entry information

Entry name

PYR5_DROME

Accession

Primary (citable) accession number: Q01637
Secondary accession number(s): Q24221, Q9VDF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project