Q01628 (IFM3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon-induced transmembrane protein 3

Alternative name(s):
Interferon-inducible protein 1-8U

Gene names

Name:

IFITM3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. Ref.3 Ref.4
Subunit structure	Interacts with SPP1; the interaction reduces OPN expression. Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Induction	By IFN-alpha and IFNG/IFN-gamma.
Post-translational modification	Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
Sequence similarities	Belongs to the CD225 family.

Ontologies

Keywords
Biological process	Antiviral defense Immunity Innate immunity
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to virus Inferred from electronic annotation. Source: UniProtKB-KW type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: LIFEdb
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 133	133	Interferon-induced transmembrane protein 3		PRO_0000153729
Regions
	Topological domain	1 – 57	57	Extracellular Potential
	Transmembrane	58 – 78	21	Helical; Potential
	Topological domain	79 – 107	29	Cytoplasmic Potential
	Transmembrane	108 – 128	21	Helical; Potential
	Topological domain	129 – 133	5	Extracellular Potential
	Region	60 – 93	34	Interaction with SPP1
Amino acid modifications
	Lipidation	71	1	S-palmitoyl cysteine Ref.6
	Lipidation	72	1	S-palmitoyl cysteine Ref.6
	Lipidation	105	1	S-palmitoyl cysteine Ref.6
Natural variations
	Natural variant	3	1	H → Q. Ref.2 Corresponds to variant rs1136853 [ dbSNP | Ensembl ].		VAR_053810
Experimental info
	Sequence conflict	2	1	N → S in CAA40626. Ref.1
	Sequence conflict	34	1	A → G in CAA40626. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01628 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 9FFB2E4623F7A1DD	FASTA	133	14,632
	10 20 30 40 50 60 MNHTVQTFFS PVNSGQPPNY EMLKEEHEVA VLGAPHNPAP PTSTVIHIRS ETSVPDHVVW 70 80 90 100 110 120 SLFNTLFMNP CCLGFIAFAY SVKSRDRKMV GDVTGAQAYA STAKCLNIWA LILGILMTIL 130 LIVIPVLIFQ AYG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of a human interferon-inducible gene family." Lewin A.R., Reid L.E., McMahon M., Stark G.R., Kerr I.M. Eur. J. Biochem. 199:417-423(1991) [PubMed: 1906403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-3. Tissue: Brain, Cervix and Vascular endothelial cell.
[3]	"The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus." Brass A.L., Huang I.C., Benita Y., John S.P., Krishnan M.N., Feeley E.M., Ryan B.J., Weyer J.L., van der Weyden L., Fikrig E., Adams D.J., Xavier R.J., Farzan M., Elledge S.J. Cell 139:1243-1254(2009) [PubMed: 20064371] [Abstract] Cited for: FUNCTION IN VIRAL RESISTANCE.
[4]	"Identification of five interferon-induced cellular proteins that inhibit west nile virus and dengue virus infections." Jiang D., Weidner J.M., Qing M., Pan X.B., Guo H., Xu C., Zhang X., Birk A., Chang J., Shi P.Y., Block T.M., Guo J.T. J. Virol. 84:8332-8341(2010) [PubMed: 20534863] [Abstract] Cited for: FUNCTION.
[5]	"Interferon-induced transmembrane 3 binds osteopontin in vitro: expressed in vivo IFITM3 reduced OPN expression." El-Tanani M.K., Jin D., Campbell F.C., Johnston P.G. Oncogene 29:752-762(2010) [PubMed: 19901966] [Abstract] Cited for: INTERACTION WITH SPP1.
[6]	"Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3." Yount J.S., Moltedo B., Yang Y.Y., Charron G., Moran T.M., Lopez C.B., Hang H.C. Nat. Chem. Biol. 6:610-614(2010) [PubMed: 20601941] [Abstract] Cited for: PALMITOYLATION AT CYS-71; CYS-72 AND CYS-105.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57352 mRNA. Translation: CAA40626.1. BC006794 mRNA. Translation: AAH06794.1. BC008417 mRNA. Translation: AAH08417.1. BC022439 mRNA. Translation: AAH22439.1. BC070243 mRNA. Translation: AAH70243.1.
IPI	IPI00303726.
PIR	S17182.
RefSeq	NP_066362.2. NM_021034.2.
UniGene	Hs.374650.
3D structure databases
ProteinModelPortal	Q01628.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-2805186.
STRING	Q01628.
PTM databases
PhosphoSite	Q01628.
Polymorphism databases
DMDM	20178301.
Proteomic databases
PRIDE	Q01628.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000399808; ENSP00000382707; ENSG00000142089.
GeneID	10410.
KEGG	hsa:10410.
UCSC	uc001lpa.2. human.
Organism-specific databases
CTD	10410.
GeneCards	GC11M000319.
H-InvDB	HIX0021368.
HGNC	HGNC:5414. IFITM3.
HPA	HPA004337.
MIM	605579. gene.
neXtProt	NX_Q01628.
PharmGKB	PA29655.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG001182.
OMA	MVIICII.
PhylomeDB	Q01628.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q01628.
Bgee	Q01628.
CleanEx	HS_IFITM3.
Genevestigator	Q01628.
Family and domain databases
InterPro	IPR007593. Interferon-induced_TM_protein. [Graphical view]
KO	K06566.
Pfam	PF04505. CD225. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39457.
SOURCE	Search...

Entry information

Entry name

IFM3_HUMAN

Accession

Primary (citable) accession number: Q01628
Secondary accession number(s): Q96HK8, Q96J15

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	April 16, 2002
Last modified:	January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.