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Protein

Membrane protein insertase MisCA

Gene

misCA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins (By similarity). Also involved in protein secretion processes. Essential for sporulation by activating sigma factor SpoIIIG/SigG after engulfment is completed in the prespore, maybe by acting on SpoIIIAE. It has an overlapping, although partly distinct, function compared to YqjG(MisCB).By similarity4 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU41040-MONOMER.

Protein family/group databases

TCDBi2.A.9.3.2. the membrane protein insertase (yidc/alb3/oxa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane protein insertase MisCA
Alternative name(s):
Foldase YidC 2
Membrane integrase YidC 2
Membrane protein YidC 2
Stage III sporulation protein J
Short name:
SpoIIIJ
Gene namesi
Name:misCA
Synonyms:spoIIIJ
Ordered Locus Names:BSU41040
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei61 – 8121HelicalSequence analysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence analysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Transmembranei204 – 22421HelicalSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential for sporulation, disruption affects the expression of prespore-specific genes but not early mother-cell-specific genes, i.e. cells do not progress beyond stage III of sporulation. A double spoIIIJ-yqjG deletion is lethal. Genetic competence increases about 20-fold in the single disruption.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 261239Membrane protein insertase MisCAPRO_0000020378Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi23 – 231N-palmitoyl cysteineSequence analysis
Lipidationi23 – 231S-diacylglycerol cysteineSequence analysis

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ01625.

Expressioni

Developmental stagei

SpoIIIJ is required only after engulfment and turned off at the onset of sporulation. While it is predominantly expressed in vegetative cells, its low expression after the onset of sporulation is essential for sporulation to occur.3 Publications

Interactioni

Subunit structurei

Mostly monomeric, it may also form dimers. Interacts with SpoIIIAE. Forms a complex with the F1F0 ATP synthase in which can be found the alpha, beta, gamma, delta and epsilon subunits of F1 and a, b and subunits of F0. YqgA is found in the same complex.2 Publications

Protein-protein interaction databases

IntActiQ01625. 10 interactions.
STRINGi224308.Bsubs1_010100022151.

Family & Domainsi

Sequence similaritiesi

Belongs to the OXA1/ALB3/YidC family. Type 2 subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0706. LUCA.
HOGENOMiHOG000009662.
InParanoidiQ01625.
KOiK03217.
OMAiKLMMAGN.
OrthoDBiEOG6X6RF2.
PhylomeDBiQ01625.

Family and domain databases

HAMAPiMF_01811. YidC_type2.
InterProiIPR028055. Membr_insert_YidC/Oxa1_C.
IPR001708. Membrane_insert_OXA1/ALB3/YidC.
IPR023060. YidC/YidC1/YidC2_Firmicutes.
[Graphical view]
PANTHERiPTHR12428. PTHR12428. 1 hit.
PfamiPF02096. 60KD_IMP. 1 hit.
[Graphical view]
PRINTSiPR00701. 60KDINNERMP.
PR01900. YIDCPROTEIN.
TIGRFAMsiTIGR03592. yidC_oxa1_cterm. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01625-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLKRRIGLL LSMVGVFMLL AGCSSVKEPI TADSPHFWDK YVVYPLSELI
60 70 80 90 100
TYVAKLTGDN YGLSIILVTI LIRLLILPLM IKQLRSSKAM QALQPEMQKL
110 120 130 140 150
KEKYSSKDQK TQQKLQQETM ALFQKHGVNP LAGCFPILIQ MPILIGFYHA
160 170 180 190 200
IMRTQAISEH SFLWFDLGEK DPYYILPIVA GVATFVQQKL MMAGNAQQNP
210 220 230 240 250
QMAMMLWIMP IMIIVFAINF PAALSLYWVV GNLFMIAQTF LIKGPDIKKN
260
PEPQKAGGKK K
Length:261
Mass (Da):29,522
Last modified:May 30, 2000 - v2
Checksum:iF211200B8CEBBEC5
GO

Sequence cautioni

The sequence CAA78595.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14225 Genomic DNA. Translation: CAA78595.1. Different initiation.
X62539 Genomic DNA. Translation: CAA44401.1.
D26185 Genomic DNA. Translation: BAA05234.1.
AL009126 Genomic DNA. Translation: CAB16141.1.
PIRiI40437.
RefSeqiNP_391984.1. NC_000964.3.
WP_010886648.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB16141; CAB16141; BSU41040.
GeneIDi937934.
KEGGibsu:BSU41040.
PATRICi18980310. VBIBacSub10457_4313.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14225 Genomic DNA. Translation: CAA78595.1. Different initiation.
X62539 Genomic DNA. Translation: CAA44401.1.
D26185 Genomic DNA. Translation: BAA05234.1.
AL009126 Genomic DNA. Translation: CAB16141.1.
PIRiI40437.
RefSeqiNP_391984.1. NC_000964.3.
WP_010886648.1. NC_000964.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ01625. 10 interactions.
STRINGi224308.Bsubs1_010100022151.

Protein family/group databases

TCDBi2.A.9.3.2. the membrane protein insertase (yidc/alb3/oxa1) family.

Proteomic databases

PaxDbiQ01625.

Protocols and materials databases

DNASUi937934.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16141; CAB16141; BSU41040.
GeneIDi937934.
KEGGibsu:BSU41040.
PATRICi18980310. VBIBacSub10457_4313.

Phylogenomic databases

eggNOGiCOG0706. LUCA.
HOGENOMiHOG000009662.
InParanoidiQ01625.
KOiK03217.
OMAiKLMMAGN.
OrthoDBiEOG6X6RF2.
PhylomeDBiQ01625.

Enzyme and pathway databases

BioCyciBSUB:BSU41040-MONOMER.

Family and domain databases

HAMAPiMF_01811. YidC_type2.
InterProiIPR028055. Membr_insert_YidC/Oxa1_C.
IPR001708. Membrane_insert_OXA1/ALB3/YidC.
IPR023060. YidC/YidC1/YidC2_Firmicutes.
[Graphical view]
PANTHERiPTHR12428. PTHR12428. 1 hit.
PfamiPF02096. 60KD_IMP. 1 hit.
[Graphical view]
PRINTSiPR00701. 60KDINNERMP.
PR01900. YIDCPROTEIN.
TIGRFAMsiTIGR03592. yidC_oxa1_cterm. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and function of the spoIIIJ gene of Bacillus subtilis: a vegetatively expressed gene that is essential for sigma G activity at an intermediate stage of sporulation."
    Errington J., Appleby L., Daniel R.A., Goodfellow H., Partridge S.R., Yudkin M.D.
    J. Gen. Microbiol. 138:2609-2618(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: 168.
  2. "Genes and their organization in the replication origin region of the bacterial chromosome."
    Ogasawara N., Yoshikawa H.
    Mol. Microbiol. 6:629-634(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / CRK2000.
  3. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "Analysis of the Bacillus subtilis spoIIIJ gene and its paralogue gene, yqjG."
    Murakami T., Haga K., Takeuchi M., Sato T.
    J. Bacteriol. 184:1998-2004(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: 168.
  6. "Complementary impact of paralogous Oxa1-like proteins of Bacillus subtilis on post-translocational stages in protein secretion."
    Tjalsma H., Bron S., van Dijl J.M.
    J. Biol. Chem. 278:15622-15632(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: 168.
  7. "Expression of spoIIIJ in the prespore is sufficient for activation of sigma G and for sporulation in Bacillus subtilis."
    Serrano M., Corte L., Opdyke J., Moran C.P. Jr., Henriques A.O.
    J. Bacteriol. 185:3905-3917(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SPORULATION.
    Strain: 168 / MB24.
  8. "Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa."
    Rubio A., Jiang X., Pogliano K.
    J. Bacteriol. 187:5000-5002(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: 168 / PY79.
  9. "Processing of a membrane protein required for cell-to-cell signaling during endospore formation in Bacillus subtilis."
    Serrano M., Vieira F., Moran C.P. Jr., Henriques A.O.
    J. Bacteriol. 190:7786-7796(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPOIIIAE, SUBUNIT, DISRUPTION PHENOTYPE.
    Strain: 168 / MB24.
  10. "Bacillus subtilis SpoIIIJ and YqjG function in membrane protein biogenesis."
    Saller M.J., Fusetti F., Driessen A.J.
    J. Bacteriol. 191:6749-6757(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH F(1)F(0) ATP SYNTHASE COMPLEX AND YQGA, COMPLEMENTS E.COLI.
    Strain: 168.
  11. "Bacillus subtilis YqjG is required for genetic competence development."
    Saller M.J., Otto A., Berrelkamp-Lahpor G.A., Becher D., Hecker M., Driessen A.J.
    Proteomics 11:270-282(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: 168.

Entry informationi

Entry nameiMISCA_BACSU
AccessioniPrimary (citable) accession number: Q01625
Secondary accession number(s): O32298
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: May 30, 2000
Last modified: February 17, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.