Reviewed, UniProtKB/Swiss-Prot Q01612 (SAT1_MESAU)

Names and origin

Protein names

Recommended name:
    Diamine acetyltransferase 1
    EC=2.3.1.57
Alternative name(s):
    Spermidine/spermine N(1)-acetyltransferase 1
      Short name=SSAT-1
      Short name=SSAT
    Putrescine acetyltransferase
    Polyamine N-acetyltransferase 1

Gene names

Name:	SAT1
Synonyms:	SAT

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Diamine acetyltransferase 1		PRO_0000074592
Regions
	Domain	4 – 171	168	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	126 – 128	3	Substrate binding By similarity
	Region	133 – 136	4	Acetyl-CoA binding By similarity
	Region	140 – 143	4	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	26	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01612-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: C2749F8506A8C6F1	FASTA	171	20,043
	10 20 30 40 50 60 MAKFKIRPAT ASDCSDILRL IKELAKYEYM EDQVMLTEKD LLEDGFGEHP FYHCLIAEVP 70 80 90 100 110 120 KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMKC 130 140 150 160 170 RCSSMHFLVA EWNEPSINFY KRRAASDLSS EEGWRLFKID KEYLLKMAAE E

References

[1]	"Nucleotide sequence of hamster spermidine/spermine-N1-acetyltransferase cDNA." Pegg A.E., Stanley B.A., Wiest L., Casero R.A. Jr. Biochim. Biophys. Acta 1171:106-108(1992) [PubMed: 1420355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z14316 mRNA. Translation: CAA78678.1.
PIR	S27157.
3D structure databases
SMR	Q01612. Positions 2-171.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q01612.
Enzyme and pathway databases
BRENDA	2.3.1.57. 824.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT1_MESAU

Accession

Primary (citable) accession number: Q01612

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	October 13, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)