Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoglycerate kinase

Gene

Pgk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (Gapdh1), Glyceraldehyde-3-phosphate dehydrogenase 2 (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (CG9010)
  2. Phosphoglycerate kinase (Pgk)
  3. no protein annotated in this organism
  4. Enolase (Eno)
  5. Pyruvate kinase (CG7362), Pyruvate kinase (CG7362), Pyruvate kinase (PyK), Pyruvate kinase (CG2964), Pyruvate kinase (CG12229), Pyruvate kinase (CG7069), Pyruvate kinase (CG7069), Pyruvate kinase (CG7362-RA), Pyruvate kinase (CG11249), Pyruvate kinase (CG2964), Pyruvate kinase (CG11249)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381SubstrateBy similarity
Binding sitei121 – 1211SubstrateBy similarity
Binding sitei169 – 1691SubstrateBy similarity
Binding sitei218 – 2181ATPBy similarity
Binding sitei311 – 3111ATP; via carbonyl oxygenBy similarity
Binding sitei342 – 3421ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi371 – 3744ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • muscle cell cellular homeostasis Source: FlyBase
  • myoblast fusion Source: FlyBase
  • phosphorylation Source: UniProtKB
  • somatic muscle development Source: FlyBase
  • synaptic transmission Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_319845. Glycolysis.
REACT_330212. Gluconeogenesis.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:Pgk
ORF Names:CG3127
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0250906. Pgk.

Subcellular locationi

GO - Cellular componenti

  • M band Source: FlyBase
  • Z disc Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Phosphoglycerate kinasePRO_0000145843Add
BLAST

Proteomic databases

PaxDbiQ01604.
PRIDEiQ01604.

Expressioni

Gene expression databases

BgeeiQ01604.
ExpressionAtlasiQ01604. differential.
GenevisibleiQ01604. DM.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi59686. 35 interactions.
IntActiQ01604. 3 interactions.
STRINGi7227.FBpp0303562.

Structurei

3D structure databases

ProteinModelPortaliQ01604.
SMRiQ01604. Positions 4-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 253Substrate bindingBy similarity
Regioni62 – 654Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0126.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000242304.
InParanoidiQ01604.
KOiK00927.
OMAiIKHCLDH.
OrthoDBiEOG74R1QN.
PhylomeDBiQ01604.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFNKLSIEN LDLAGKRVLM RVDFNVPIKE GKITSNQRIV AALDSIKLAL
60 70 80 90 100
SKKAKSVVLM SHLGRPDGNK NIKYTLAPVA AELKTLLGQD VIFLSDCVGS
110 120 130 140 150
EVEAACKDPA PGSVILLENV RFYVEEEGKG LDASGGKVKA DPAKVKEFRA
160 170 180 190 200
SLAKLGDVYV NDAFGTAHRA HSSMMGDGFE QRAAGLLLNK ELKYFSQALD
210 220 230 240 250
KPPNPFLAIL GGAKVADKIQ LIENLLDKVN EMIIGGGMAF TFLKVLNNMK
260 270 280 290 300
IGGSLFDEEG SKIVEKLVEK AKKNNVQLHL PVDFVCGDKF AENAAVSEAT
310 320 330 340 350
VEAGIPDGHM GLDVGPKTRE LFAAPIARAK LIVWNGPPGV FEFPNFANGT
360 370 380 390 400
KSIMDGVVAA TKNGTVSIIG GGDTASCCAK WNTEALVSHV STGGGASLEL
410
LEGKTLPGVA ALTSA
Length:415
Mass (Da):43,862
Last modified:June 1, 2001 - v2
Checksum:i7D1225FCC81F8B42
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti410 – 4101A → R in CAA78404 (PubMed:1465095).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti388 – 3881S → A in strain: HFL97_3e2. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14029 Genomic DNA. Translation: CAA78404.1.
DQ863952 Genomic DNA. Translation: ABH06587.1.
DQ863953 Genomic DNA. Translation: ABH06588.1.
DQ863954 Genomic DNA. Translation: ABH06589.1.
DQ863955 Genomic DNA. Translation: ABH06590.1.
DQ863956 Genomic DNA. Translation: ABH06591.1.
DQ863957 Genomic DNA. Translation: ABH06592.1.
DQ863958 Genomic DNA. Translation: ABH06593.1.
DQ863959 Genomic DNA. Translation: ABH06594.1.
DQ863960 Genomic DNA. Translation: ABH06595.1.
DQ863961 Genomic DNA. Translation: ABH06596.1.
DQ863962 Genomic DNA. Translation: ABH06597.1.
DQ863963 Genomic DNA. Translation: ABH06598.1.
DQ863964 Genomic DNA. Translation: ABH06599.1.
DQ863965 Genomic DNA. Translation: ABH06600.1.
DQ863966 Genomic DNA. Translation: ABH06601.1.
DQ863967 Genomic DNA. Translation: ABH06602.1.
DQ863968 Genomic DNA. Translation: ABH06603.1.
DQ863969 Genomic DNA. Translation: ABH06604.1.
DQ863970 Genomic DNA. Translation: ABH06605.1.
DQ863971 Genomic DNA. Translation: ABH06606.1.
DQ863972 Genomic DNA. Translation: ABH06607.1.
DQ863973 Genomic DNA. Translation: ABH06608.1.
DQ863974 Genomic DNA. Translation: ABH06609.1.
DQ863975 Genomic DNA. Translation: ABH06610.1.
DQ863976 Genomic DNA. Translation: ABH06611.1.
AE014134 Genomic DNA. Translation: AAF51218.1.
BT023910 mRNA. Translation: ABA81844.1.
PIRiS30111. KIFFPG.
RefSeqiNP_001259956.1. NM_001273027.1.
NP_001259957.1. NM_001273028.1.
NP_001259958.1. NM_001273029.1.
NP_001259959.1. NM_001273030.1.
NP_476676.1. NM_057328.4.
UniGeneiDm.6415.

Genome annotation databases

EnsemblMetazoaiFBtr0077739; FBpp0077419; FBgn0250906.
FBtr0330716; FBpp0303560; FBgn0250906.
FBtr0330717; FBpp0303561; FBgn0250906.
FBtr0330718; FBpp0303562; FBgn0250906.
FBtr0334162; FBpp0306279; FBgn0250906.
GeneIDi33461.
KEGGidme:Dmel_CG3127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14029 Genomic DNA. Translation: CAA78404.1.
DQ863952 Genomic DNA. Translation: ABH06587.1.
DQ863953 Genomic DNA. Translation: ABH06588.1.
DQ863954 Genomic DNA. Translation: ABH06589.1.
DQ863955 Genomic DNA. Translation: ABH06590.1.
DQ863956 Genomic DNA. Translation: ABH06591.1.
DQ863957 Genomic DNA. Translation: ABH06592.1.
DQ863958 Genomic DNA. Translation: ABH06593.1.
DQ863959 Genomic DNA. Translation: ABH06594.1.
DQ863960 Genomic DNA. Translation: ABH06595.1.
DQ863961 Genomic DNA. Translation: ABH06596.1.
DQ863962 Genomic DNA. Translation: ABH06597.1.
DQ863963 Genomic DNA. Translation: ABH06598.1.
DQ863964 Genomic DNA. Translation: ABH06599.1.
DQ863965 Genomic DNA. Translation: ABH06600.1.
DQ863966 Genomic DNA. Translation: ABH06601.1.
DQ863967 Genomic DNA. Translation: ABH06602.1.
DQ863968 Genomic DNA. Translation: ABH06603.1.
DQ863969 Genomic DNA. Translation: ABH06604.1.
DQ863970 Genomic DNA. Translation: ABH06605.1.
DQ863971 Genomic DNA. Translation: ABH06606.1.
DQ863972 Genomic DNA. Translation: ABH06607.1.
DQ863973 Genomic DNA. Translation: ABH06608.1.
DQ863974 Genomic DNA. Translation: ABH06609.1.
DQ863975 Genomic DNA. Translation: ABH06610.1.
DQ863976 Genomic DNA. Translation: ABH06611.1.
AE014134 Genomic DNA. Translation: AAF51218.1.
BT023910 mRNA. Translation: ABA81844.1.
PIRiS30111. KIFFPG.
RefSeqiNP_001259956.1. NM_001273027.1.
NP_001259957.1. NM_001273028.1.
NP_001259958.1. NM_001273029.1.
NP_001259959.1. NM_001273030.1.
NP_476676.1. NM_057328.4.
UniGeneiDm.6415.

3D structure databases

ProteinModelPortaliQ01604.
SMRiQ01604. Positions 4-414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59686. 35 interactions.
IntActiQ01604. 3 interactions.
STRINGi7227.FBpp0303562.

Proteomic databases

PaxDbiQ01604.
PRIDEiQ01604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077739; FBpp0077419; FBgn0250906.
FBtr0330716; FBpp0303560; FBgn0250906.
FBtr0330717; FBpp0303561; FBgn0250906.
FBtr0330718; FBpp0303562; FBgn0250906.
FBtr0334162; FBpp0306279; FBgn0250906.
GeneIDi33461.
KEGGidme:Dmel_CG3127.

Organism-specific databases

CTDi33461.
FlyBaseiFBgn0250906. Pgk.

Phylogenomic databases

eggNOGiCOG0126.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000242304.
InParanoidiQ01604.
KOiK00927.
OMAiIKHCLDH.
OrthoDBiEOG74R1QN.
PhylomeDBiQ01604.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
ReactomeiREACT_319845. Glycolysis.
REACT_330212. Gluconeogenesis.

Miscellaneous databases

GenomeRNAii33461.
NextBioi783688.
PROiQ01604.

Gene expression databases

BgeeiQ01604.
ExpressionAtlasiQ01604. differential.
GenevisibleiQ01604. DM.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster."
    Roselli-Rehfuss L., Ye F., Lissemore J.L., Sullivan D.T.
    Mol. Gen. Genet. 235:213-220(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-388.
    Strain: DPF96_10, DPF96_26, DPF96_5.1, DPF96_8.3, HFL97_15e, HFL97_23e, HFL97_3e2, HFL97_61e, HFL97_68e, MA96_24.2, MA96_26.4, MA96_3.5, MA96_4.4, MA96_40.1, MA96_42.4, MA96_49.2, MA96_9.3, Z(H)_26e2, Z(H)_28e2, Z(H)_36e2, Z(H)_38e2, Z(H)_44e2, Z(S)_11e2, Z(S)_28e2 and Z(S)_49e2.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiPGK_DROME
AccessioniPrimary (citable) accession number: Q01604
Secondary accession number(s): A5XCG6, Q3KN29, Q9VQF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.