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Q01604

- PGK_DROME

UniProt

Q01604 - PGK_DROME

Protein

Phosphoglycerate kinase

Gene

Pgk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381SubstrateBy similarity
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei169 – 1691SubstrateBy similarity
    Binding sitei218 – 2181ATPBy similarity
    Binding sitei311 – 3111ATP; via carbonyl oxygenBy similarity
    Binding sitei342 – 3421ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi371 – 3744ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. phosphoglycerate kinase activity Source: UniProtKB

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. muscle cell cellular homeostasis Source: FlyBase
    3. phosphorylation Source: UniProtKB
    4. synaptic transmission Source: FlyBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_206319. Glycolysis.
    REACT_220315. Gluconeogenesis.
    UniPathwayiUPA00109; UER00185.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglycerate kinase (EC:2.7.2.3)
    Gene namesi
    Name:Pgk
    ORF Names:CG3127
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0250906. Pgk.

    Subcellular locationi

    GO - Cellular componenti

    1. M band Source: FlyBase
    2. Z disc Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415Phosphoglycerate kinasePRO_0000145843Add
    BLAST

    Proteomic databases

    PaxDbiQ01604.
    PRIDEiQ01604.

    Expressioni

    Gene expression databases

    BgeeiQ01604.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi59686. 35 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01604.
    SMRiQ01604. Positions 4-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni23 – 253Substrate bindingBy similarity
    Regioni62 – 654Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the phosphoglycerate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0126.
    GeneTreeiENSGT00390000008820.
    HOGENOMiHOG000242304.
    InParanoidiQ01604.
    KOiK00927.
    OMAiASCCAKW.
    OrthoDBiEOG74R1QN.
    PhylomeDBiQ01604.

    Family and domain databases

    Gene3Di3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPiMF_00145. Phosphoglyc_kinase.
    InterProiIPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view]
    PANTHERiPTHR11406. PTHR11406. 1 hit.
    PfamiPF00162. PGK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000724. Pgk. 1 hit.
    PRINTSiPR00477. PHGLYCKINASE.
    SUPFAMiSSF53748. SSF53748. 1 hit.
    PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01604-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFNKLSIEN LDLAGKRVLM RVDFNVPIKE GKITSNQRIV AALDSIKLAL    50
    SKKAKSVVLM SHLGRPDGNK NIKYTLAPVA AELKTLLGQD VIFLSDCVGS 100
    EVEAACKDPA PGSVILLENV RFYVEEEGKG LDASGGKVKA DPAKVKEFRA 150
    SLAKLGDVYV NDAFGTAHRA HSSMMGDGFE QRAAGLLLNK ELKYFSQALD 200
    KPPNPFLAIL GGAKVADKIQ LIENLLDKVN EMIIGGGMAF TFLKVLNNMK 250
    IGGSLFDEEG SKIVEKLVEK AKKNNVQLHL PVDFVCGDKF AENAAVSEAT 300
    VEAGIPDGHM GLDVGPKTRE LFAAPIARAK LIVWNGPPGV FEFPNFANGT 350
    KSIMDGVVAA TKNGTVSIIG GGDTASCCAK WNTEALVSHV STGGGASLEL 400
    LEGKTLPGVA ALTSA 415
    Length:415
    Mass (Da):43,862
    Last modified:June 1, 2001 - v2
    Checksum:i7D1225FCC81F8B42
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti410 – 4101A → R in CAA78404. (PubMed:1465095)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti388 – 3881S → A in strain: HFL97_3e2. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14029 Genomic DNA. Translation: CAA78404.1.
    DQ863952 Genomic DNA. Translation: ABH06587.1.
    DQ863953 Genomic DNA. Translation: ABH06588.1.
    DQ863954 Genomic DNA. Translation: ABH06589.1.
    DQ863955 Genomic DNA. Translation: ABH06590.1.
    DQ863956 Genomic DNA. Translation: ABH06591.1.
    DQ863957 Genomic DNA. Translation: ABH06592.1.
    DQ863958 Genomic DNA. Translation: ABH06593.1.
    DQ863959 Genomic DNA. Translation: ABH06594.1.
    DQ863960 Genomic DNA. Translation: ABH06595.1.
    DQ863961 Genomic DNA. Translation: ABH06596.1.
    DQ863962 Genomic DNA. Translation: ABH06597.1.
    DQ863963 Genomic DNA. Translation: ABH06598.1.
    DQ863964 Genomic DNA. Translation: ABH06599.1.
    DQ863965 Genomic DNA. Translation: ABH06600.1.
    DQ863966 Genomic DNA. Translation: ABH06601.1.
    DQ863967 Genomic DNA. Translation: ABH06602.1.
    DQ863968 Genomic DNA. Translation: ABH06603.1.
    DQ863969 Genomic DNA. Translation: ABH06604.1.
    DQ863970 Genomic DNA. Translation: ABH06605.1.
    DQ863971 Genomic DNA. Translation: ABH06606.1.
    DQ863972 Genomic DNA. Translation: ABH06607.1.
    DQ863973 Genomic DNA. Translation: ABH06608.1.
    DQ863974 Genomic DNA. Translation: ABH06609.1.
    DQ863975 Genomic DNA. Translation: ABH06610.1.
    DQ863976 Genomic DNA. Translation: ABH06611.1.
    AE014134 Genomic DNA. Translation: AAF51218.1.
    BT023910 mRNA. Translation: ABA81844.1.
    PIRiS30111. KIFFPG.
    RefSeqiNP_001259956.1. NM_001273027.1.
    NP_001259957.1. NM_001273028.1.
    NP_001259958.1. NM_001273029.1.
    NP_001259959.1. NM_001273030.1.
    NP_476676.1. NM_057328.4.
    UniGeneiDm.6415.

    Genome annotation databases

    EnsemblMetazoaiFBtr0077739; FBpp0077419; FBgn0250906.
    FBtr0330716; FBpp0303560; FBgn0250906.
    FBtr0330717; FBpp0303561; FBgn0250906.
    FBtr0330718; FBpp0303562; FBgn0250906.
    GeneIDi33461.
    KEGGidme:Dmel_CG3127.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14029 Genomic DNA. Translation: CAA78404.1 .
    DQ863952 Genomic DNA. Translation: ABH06587.1 .
    DQ863953 Genomic DNA. Translation: ABH06588.1 .
    DQ863954 Genomic DNA. Translation: ABH06589.1 .
    DQ863955 Genomic DNA. Translation: ABH06590.1 .
    DQ863956 Genomic DNA. Translation: ABH06591.1 .
    DQ863957 Genomic DNA. Translation: ABH06592.1 .
    DQ863958 Genomic DNA. Translation: ABH06593.1 .
    DQ863959 Genomic DNA. Translation: ABH06594.1 .
    DQ863960 Genomic DNA. Translation: ABH06595.1 .
    DQ863961 Genomic DNA. Translation: ABH06596.1 .
    DQ863962 Genomic DNA. Translation: ABH06597.1 .
    DQ863963 Genomic DNA. Translation: ABH06598.1 .
    DQ863964 Genomic DNA. Translation: ABH06599.1 .
    DQ863965 Genomic DNA. Translation: ABH06600.1 .
    DQ863966 Genomic DNA. Translation: ABH06601.1 .
    DQ863967 Genomic DNA. Translation: ABH06602.1 .
    DQ863968 Genomic DNA. Translation: ABH06603.1 .
    DQ863969 Genomic DNA. Translation: ABH06604.1 .
    DQ863970 Genomic DNA. Translation: ABH06605.1 .
    DQ863971 Genomic DNA. Translation: ABH06606.1 .
    DQ863972 Genomic DNA. Translation: ABH06607.1 .
    DQ863973 Genomic DNA. Translation: ABH06608.1 .
    DQ863974 Genomic DNA. Translation: ABH06609.1 .
    DQ863975 Genomic DNA. Translation: ABH06610.1 .
    DQ863976 Genomic DNA. Translation: ABH06611.1 .
    AE014134 Genomic DNA. Translation: AAF51218.1 .
    BT023910 mRNA. Translation: ABA81844.1 .
    PIRi S30111. KIFFPG.
    RefSeqi NP_001259956.1. NM_001273027.1.
    NP_001259957.1. NM_001273028.1.
    NP_001259958.1. NM_001273029.1.
    NP_001259959.1. NM_001273030.1.
    NP_476676.1. NM_057328.4.
    UniGenei Dm.6415.

    3D structure databases

    ProteinModelPortali Q01604.
    SMRi Q01604. Positions 4-414.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 59686. 35 interactions.

    Proteomic databases

    PaxDbi Q01604.
    PRIDEi Q01604.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0077739 ; FBpp0077419 ; FBgn0250906 .
    FBtr0330716 ; FBpp0303560 ; FBgn0250906 .
    FBtr0330717 ; FBpp0303561 ; FBgn0250906 .
    FBtr0330718 ; FBpp0303562 ; FBgn0250906 .
    GeneIDi 33461.
    KEGGi dme:Dmel_CG3127.

    Organism-specific databases

    CTDi 33461.
    FlyBasei FBgn0250906. Pgk.

    Phylogenomic databases

    eggNOGi COG0126.
    GeneTreei ENSGT00390000008820.
    HOGENOMi HOG000242304.
    InParanoidi Q01604.
    KOi K00927.
    OMAi ASCCAKW.
    OrthoDBi EOG74R1QN.
    PhylomeDBi Q01604.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00185 .
    Reactomei REACT_206319. Glycolysis.
    REACT_220315. Gluconeogenesis.

    Miscellaneous databases

    ChiTaRSi PRKG1. drosophila.
    GenomeRNAii 33461.
    NextBioi 783688.

    Gene expression databases

    Bgeei Q01604.

    Family and domain databases

    Gene3Di 3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPi MF_00145. Phosphoglyc_kinase.
    InterProi IPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view ]
    PANTHERi PTHR11406. PTHR11406. 1 hit.
    Pfami PF00162. PGK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000724. Pgk. 1 hit.
    PRINTSi PR00477. PHGLYCKINASE.
    SUPFAMi SSF53748. SSF53748. 1 hit.
    PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster."
      Roselli-Rehfuss L., Ye F., Lissemore J.L., Sullivan D.T.
      Mol. Gen. Genet. 235:213-220(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oregon-R.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-388.
      Strain: DPF96_10, DPF96_26, DPF96_5.1, DPF96_8.3, HFL97_15e, HFL97_23e, HFL97_3e2, HFL97_61e, HFL97_68e, MA96_24.2, MA96_26.4, MA96_3.5, MA96_4.4, MA96_40.1, MA96_42.4, MA96_49.2, MA96_9.3, Z(H)_26e2, Z(H)_28e2, Z(H)_36e2, Z(H)_38e2, Z(H)_44e2, Z(S)_11e2, Z(S)_28e2 and Z(S)_49e2.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Head.

    Entry informationi

    Entry nameiPGK_DROME
    AccessioniPrimary (citable) accession number: Q01604
    Secondary accession number(s): A5XCG6, Q3KN29, Q9VQF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3