Q01604 (PGK_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	Pgk
ORF Names:	CG3127

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway muscle cell homeostasis Inferred from genetic interaction PubMed 21256839. Source: FlyBase synaptic transmission Inferred from direct assay PubMed 15140922. Source: FlyBase
Cellular_component	M band Inferred from direct assay PubMed 12756285. Source: FlyBase Z disc Inferred from direct assay PubMed 12756285. Source: FlyBase
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB phosphoglycerate kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Phosphoglycerate kinase		PRO_0000145843
Regions
	Nucleotide binding	371 – 374	4	ATP By similarity
	Region	23 – 25	3	Substrate binding By similarity
	Region	62 – 65	4	Substrate binding By similarity
Sites
	Binding site	38	1	Substrate By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	218	1	ATP By similarity
	Binding site	311	1	ATP; via carbonyl oxygen By similarity
	Binding site	342	1	ATP By similarity
Natural variations
	Natural variant	388	1	S → A in strain: HFL97_3e2. Ref.2
Experimental info
	Sequence conflict	410	1	A → R in CAA78404. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01604 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 7D1225FCC81F8B42	FASTA	415	43,862
	10 20 30 40 50 60 MAFNKLSIEN LDLAGKRVLM RVDFNVPIKE GKITSNQRIV AALDSIKLAL SKKAKSVVLM 70 80 90 100 110 120 SHLGRPDGNK NIKYTLAPVA AELKTLLGQD VIFLSDCVGS EVEAACKDPA PGSVILLENV 130 140 150 160 170 180 RFYVEEEGKG LDASGGKVKA DPAKVKEFRA SLAKLGDVYV NDAFGTAHRA HSSMMGDGFE 190 200 210 220 230 240 QRAAGLLLNK ELKYFSQALD KPPNPFLAIL GGAKVADKIQ LIENLLDKVN EMIIGGGMAF 250 260 270 280 290 300 TFLKVLNNMK IGGSLFDEEG SKIVEKLVEK AKKNNVQLHL PVDFVCGDKF AENAAVSEAT 310 320 330 340 350 360 VEAGIPDGHM GLDVGPKTRE LFAAPIARAK LIVWNGPPGV FEFPNFANGT KSIMDGVVAA 370 380 390 400 410 TKNGTVSIIG GGDTASCCAK WNTEALVSHV STGGGASLEL LEGKTLPGVA ALTSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster." Roselli-Rehfuss L., Ye F., Lissemore J.L., Sullivan D.T. Mol. Gen. Genet. 235:213-220(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-R.
[2]	"Adaptive evolution of metabolic pathways in Drosophila." Flowers J., Sezgin E., Kumagai S., Duvernell D., Matzkin L., Schmidt P., Eanes W. Mol. Biol. Evol. 24:1347-1354(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-388. Strain: DPF96_10, DPF96_26, DPF96_5.1, DPF96_8.3, HFL97_15e, HFL97_23e, HFL97_3e2, HFL97_61e, HFL97_68e, MA96_24.2, MA96_26.4, MA96_3.5, MA96_4.4, MA96_40.1, MA96_42.4, MA96_49.2, MA96_9.3, Z(H)_26e2, Z(H)_28e2, Z(H)_36e2, Z(H)_38e2, Z(H)_44e2, Z(S)_11e2, Z(S)_28e2 and Z(S)_49e2.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z14029 Genomic DNA. Translation: CAA78404.1. DQ863952 Genomic DNA. Translation: ABH06587.1. DQ863953 Genomic DNA. Translation: ABH06588.1. DQ863954 Genomic DNA. Translation: ABH06589.1. DQ863955 Genomic DNA. Translation: ABH06590.1. DQ863956 Genomic DNA. Translation: ABH06591.1. DQ863957 Genomic DNA. Translation: ABH06592.1. DQ863958 Genomic DNA. Translation: ABH06593.1. DQ863959 Genomic DNA. Translation: ABH06594.1. DQ863960 Genomic DNA. Translation: ABH06595.1. DQ863961 Genomic DNA. Translation: ABH06596.1. DQ863962 Genomic DNA. Translation: ABH06597.1. DQ863963 Genomic DNA. Translation: ABH06598.1. DQ863964 Genomic DNA. Translation: ABH06599.1. DQ863965 Genomic DNA. Translation: ABH06600.1. DQ863966 Genomic DNA. Translation: ABH06601.1. DQ863967 Genomic DNA. Translation: ABH06602.1. DQ863968 Genomic DNA. Translation: ABH06603.1. DQ863969 Genomic DNA. Translation: ABH06604.1. DQ863970 Genomic DNA. Translation: ABH06605.1. DQ863971 Genomic DNA. Translation: ABH06606.1. DQ863972 Genomic DNA. Translation: ABH06607.1. DQ863973 Genomic DNA. Translation: ABH06608.1. DQ863974 Genomic DNA. Translation: ABH06609.1. DQ863975 Genomic DNA. Translation: ABH06610.1. DQ863976 Genomic DNA. Translation: ABH06611.1. AE014134 Genomic DNA. Translation: AAF51218.1. BT023910 mRNA. Translation: ABA81844.1.
PIR	KIFFPG. S30111.
RefSeq	NP_001259956.1. NM_001273027.1. NP_001259957.1. NM_001273028.1. NP_001259958.1. NM_001273029.1. NP_001259959.1. NM_001273030.1. NP_476676.1. NM_057328.4.
UniGene	Dm.6415.
3D structure databases
ProteinModelPortal	Q01604.
SMR	Q01604. Positions 4-414.
ModBase	Search...
Proteomic databases
PaxDb	Q01604.
PRIDE	Q01604.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0077739; FBpp0077419; FBgn0250906. FBtr0330716; FBpp0303560; FBgn0250906. FBtr0330717; FBpp0303561; FBgn0250906. FBtr0330718; FBpp0303562; FBgn0250906.
GeneID	33461.
KEGG	dme:Dmel_CG3127.
Organism-specific databases
CTD	33461.
FlyBase	FBgn0250906. Pgk.
Phylogenomic databases
eggNOG	COG0126.
GeneTree	ENSGT00390000008820.
HOGENOM	HOG000242304.
InParanoid	Q01604.
KO	K00927.
OMA	NFANGTK.
OrthoDB	EOG4MGQPT.
PhylomeDB	Q01604.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Gene expression databases
Bgee	Q01604.
GermOnline	CG3127. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PRKG1. drosophila.
GenomeRNAi	33461.
NextBio	783688.

Entry information

Entry name

PGK_DROME

Accession

Primary (citable) accession number: Q01604
Secondary accession number(s): A5XCG6, Q3KN29, Q9VQF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project