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Q01604 (PGK_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:Pgk
ORF Names:CG3127
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145843

Regions

Nucleotide binding371 – 3744ATP By similarity
Region23 – 253Substrate binding By similarity
Region62 – 654Substrate binding By similarity

Sites

Binding site381Substrate By similarity
Binding site1211Substrate By similarity
Binding site1691Substrate By similarity
Binding site2181ATP By similarity
Binding site3111ATP; via carbonyl oxygen By similarity
Binding site3421ATP By similarity

Natural variations

Natural variant3881S → A in strain: HFL97_3e2. Ref.2

Experimental info

Sequence conflict4101A → R in CAA78404. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q01604 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 7D1225FCC81F8B42

FASTA41543,862
        10         20         30         40         50         60 
MAFNKLSIEN LDLAGKRVLM RVDFNVPIKE GKITSNQRIV AALDSIKLAL SKKAKSVVLM 

        70         80         90        100        110        120 
SHLGRPDGNK NIKYTLAPVA AELKTLLGQD VIFLSDCVGS EVEAACKDPA PGSVILLENV 

       130        140        150        160        170        180 
RFYVEEEGKG LDASGGKVKA DPAKVKEFRA SLAKLGDVYV NDAFGTAHRA HSSMMGDGFE 

       190        200        210        220        230        240 
QRAAGLLLNK ELKYFSQALD KPPNPFLAIL GGAKVADKIQ LIENLLDKVN EMIIGGGMAF 

       250        260        270        280        290        300 
TFLKVLNNMK IGGSLFDEEG SKIVEKLVEK AKKNNVQLHL PVDFVCGDKF AENAAVSEAT 

       310        320        330        340        350        360 
VEAGIPDGHM GLDVGPKTRE LFAAPIARAK LIVWNGPPGV FEFPNFANGT KSIMDGVVAA 

       370        380        390        400        410 
TKNGTVSIIG GGDTASCCAK WNTEALVSHV STGGGASLEL LEGKTLPGVA ALTSA 

« Hide

References

« Hide 'large scale' references
[1]"Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster."
Roselli-Rehfuss L., Ye F., Lissemore J.L., Sullivan D.T.
Mol. Gen. Genet. 235:213-220(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Oregon-R.
[2]"Adaptive evolution of metabolic pathways in Drosophila."
Flowers J., Sezgin E., Kumagai S., Duvernell D., Matzkin L., Schmidt P., Eanes W.
Mol. Biol. Evol. 24:1347-1354(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-388.
Strain: DPF96_10, DPF96_26, DPF96_5.1, DPF96_8.3, HFL97_15e, HFL97_23e, HFL97_3e2, HFL97_61e, HFL97_68e, MA96_24.2, MA96_26.4, MA96_3.5, MA96_4.4, MA96_40.1, MA96_42.4, MA96_49.2, MA96_9.3, Z(H)_26e2, Z(H)_28e2, Z(H)_36e2, Z(H)_38e2, Z(H)_44e2, Z(S)_11e2, Z(S)_28e2 and Z(S)_49e2.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Head.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z14029 Genomic DNA. Translation: CAA78404.1.
DQ863952 Genomic DNA. Translation: ABH06587.1.
DQ863953 Genomic DNA. Translation: ABH06588.1.
DQ863954 Genomic DNA. Translation: ABH06589.1.
DQ863955 Genomic DNA. Translation: ABH06590.1.
DQ863956 Genomic DNA. Translation: ABH06591.1.
DQ863957 Genomic DNA. Translation: ABH06592.1.
DQ863958 Genomic DNA. Translation: ABH06593.1.
DQ863959 Genomic DNA. Translation: ABH06594.1.
DQ863960 Genomic DNA. Translation: ABH06595.1.
DQ863961 Genomic DNA. Translation: ABH06596.1.
DQ863962 Genomic DNA. Translation: ABH06597.1.
DQ863963 Genomic DNA. Translation: ABH06598.1.
DQ863964 Genomic DNA. Translation: ABH06599.1.
DQ863965 Genomic DNA. Translation: ABH06600.1.
DQ863966 Genomic DNA. Translation: ABH06601.1.
DQ863967 Genomic DNA. Translation: ABH06602.1.
DQ863968 Genomic DNA. Translation: ABH06603.1.
DQ863969 Genomic DNA. Translation: ABH06604.1.
DQ863970 Genomic DNA. Translation: ABH06605.1.
DQ863971 Genomic DNA. Translation: ABH06606.1.
DQ863972 Genomic DNA. Translation: ABH06607.1.
DQ863973 Genomic DNA. Translation: ABH06608.1.
DQ863974 Genomic DNA. Translation: ABH06609.1.
DQ863975 Genomic DNA. Translation: ABH06610.1.
DQ863976 Genomic DNA. Translation: ABH06611.1.
AE014134 Genomic DNA. Translation: AAF51218.1.
BT023910 mRNA. Translation: ABA81844.1.
PIRKIFFPG. S30111.
RefSeqNP_001259956.1. NM_001273027.1.
NP_001259957.1. NM_001273028.1.
NP_001259958.1. NM_001273029.1.
NP_001259959.1. NM_001273030.1.
NP_476676.1. NM_057328.4.
UniGeneDm.6415.

3D structure databases

ProteinModelPortalQ01604.
SMRQ01604. Positions 4-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid59686. 35 interactions.

Proteomic databases

PaxDbQ01604.
PRIDEQ01604.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0077739; FBpp0077419; FBgn0250906.
FBtr0330716; FBpp0303560; FBgn0250906.
FBtr0330717; FBpp0303561; FBgn0250906.
FBtr0330718; FBpp0303562; FBgn0250906.
GeneID33461.
KEGGdme:Dmel_CG3127.

Organism-specific databases

CTD33461.
FlyBaseFBgn0250906. Pgk.

Phylogenomic databases

eggNOGCOG0126.
GeneTreeENSGT00390000008820.
HOGENOMHOG000242304.
InParanoidQ01604.
KOK00927.
OMAASCCAKW.
OrthoDBEOG74R1QN.
PhylomeDBQ01604.

Enzyme and pathway databases

UniPathwayUPA00109; UER00185.

Gene expression databases

BgeeQ01604.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPRKG1. drosophila.
GenomeRNAi33461.
NextBio783688.

Entry information

Entry namePGK_DROME
AccessionPrimary (citable) accession number: Q01604
Secondary accession number(s): A5XCG6, Q3KN29, Q9VQF6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase