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Protein

Peroxidase

Gene

Pxd

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the chorion hardening process, through protein cross-linking mediated by the formation of di- and tri-tyrosine bonds.1 Publication

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185Proton acceptorPROSITE-ProRule annotation1
Sitei331Transition state stabilizerPROSITE-ProRule annotation1
Metal bindingi437Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: FlyBase

GO - Biological processi

  • eggshell chorion assembly Source: FlyBase
  • hydrogen peroxide catabolic process Source: UniProtKB-KW
  • neurogenesis Source: FlyBase
  • phagocytosis Source: FlyBase
  • response to ethanol Source: FlyBase
  • response to oxidative stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-209968. Thyroxine biosynthesis.
R-DME-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei4118. DmPxt02-A.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase (EC:1.11.1.7)
Short name:
DmPO
Alternative name(s):
Chorion peroxidase
Gene namesi
Name:Pxd
Synonyms:HDC14047, PO
ORF Names:CG3477
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0004577. Pxd.

Subcellular locationi

GO - Cellular componenti

  • chorion Source: FlyBase
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002363821 – 690PeroxidaseAdd BLAST670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi100 ↔ 112PROSITE-ProRule annotation
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi315 ↔ 324PROSITE-ProRule annotation
Disulfide bondi536 ↔ 592PROSITE-ProRule annotation
Disulfide bondi636 ↔ 662PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ01603.
PRIDEiQ01603.

Expressioni

Gene expression databases

BgeeiFBgn0004577.
ExpressionAtlasiQ01603. baseline.
GenevisibleiQ01603. DM.

Interactioni

Protein-protein interaction databases

IntActiQ01603. 1 interactor.
STRINGi7227.FBpp0088343.

Structurei

3D structure databases

ProteinModelPortaliQ01603.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2408. Eukaryota.
ENOG410XPZ3. LUCA.
GeneTreeiENSGT00550000074325.
InParanoidiQ01603.
OMAiLHNMQWG.
OrthoDBiEOG091G00D8.
PhylomeDBiQ01603.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR029585. Pxd_insect.
[Graphical view]
PANTHERiPTHR11475:SF65. PTHR11475:SF65. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS50292. PEROXIDASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRARDLLLL ALLGFISSAL GLKVSSGYHI VHNQPQSSFP NYHGFSYLQG
60 70 80 90 100
SAPYVIGNSL PTSPAPQNPF SSPASPPVSA YGYSFPTAGR VSCAAPPAVC
110 120 130 140 150
EKTAYRTLDG SCNHLEQPGL GVANSKYGRL LTPKYADGIS APTRSVTGDE
160 170 180 190 200
LPSARLVSLV AFGEQDVPDP EFTLHNMQWG QIMTHDMSMQ AGGTQSKKHP
210 220 230 240 250
TRCCTDDGRL IGLDTAHKTC FAIIVPPHDP AYSQVGTECL NFVRTLTDRD
260 270 280 290 300
SNCQYQGGPA EQLTVVTSYL DLSLVYGNSI QQNSDIREFQ GGRMIVEERN
310 320 330 340 350
GAKWLPLSRN VTGDCDAVDA SEVCYRSGDV RVNQNPGLAI LQTILLREHN
360 370 380 390 400
RIADALSALN PHYDDRTLFQ EARKINIAQY QQISYYEWLP IFLGGENMLK
410 420 430 440 450
NRLIYKAPSG SYINDFDPNI DPSVLNEHAT AAFRYFHSQI EGRLDLLSEL
460 470 480 490 500
RQVLGSLTLS DWFNRPGIIE VGDNFDSLTR GHATQPEELT DINFDRQIKH
510 520 530 540 550
FLFRRNMPFG SDLRSLDIQR NRDHGLASYN DMREFCGLRR AHSWEGYGDL
560 570 580 590 600
ISPPILEKLK SLYPSHEDVD LTVGASLEAH VAGALAGPTF LCILTEQFYR
610 620 630 640 650
TRVGDRFFFE NGDKLTGFTP DQLEELRKAS MARLLCDNGN HISSMQPEAF
660 670 680 690
RTVSHSNPII PCSNIPQVDL TKWIDQKLYA TVDPSHYGKK
Length:690
Mass (Da):76,719
Last modified:March 2, 2010 - v2
Checksum:i51BC6F450A8773C0
GO

Sequence cautioni

The sequence DAA04104 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti584A → T in CAA48238 (PubMed:1482687).Curated1
Sequence conflicti584A → T in AAS48542 (PubMed:15979004).Curated1
Sequence conflicti678L → P in CAA48238 (PubMed:1482687).Curated1
Sequence conflicti678L → P in AAS48542 (PubMed:15979004).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68131 Genomic DNA. Translation: CAA48238.1.
AY541497 mRNA. Translation: AAS48542.1.
AE014297 Genomic DNA. Translation: AAS65161.1.
BK002598 Genomic DNA. Translation: DAA04104.1. Different initiation.
PIRiS28222.
RefSeqiNP_001163633.1. NM_001170162.2.
NP_996223.1. NM_206501.3.
UniGeneiDm.31257.

Genome annotation databases

EnsemblMetazoaiFBtr0089287; FBpp0088343; FBgn0004577.
FBtr0301526; FBpp0290741; FBgn0004577.
GeneIDi2768671.
KEGGidme:Dmel_CG3477.
UCSCiCG3477-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68131 Genomic DNA. Translation: CAA48238.1.
AY541497 mRNA. Translation: AAS48542.1.
AE014297 Genomic DNA. Translation: AAS65161.1.
BK002598 Genomic DNA. Translation: DAA04104.1. Different initiation.
PIRiS28222.
RefSeqiNP_001163633.1. NM_001170162.2.
NP_996223.1. NM_206501.3.
UniGeneiDm.31257.

3D structure databases

ProteinModelPortaliQ01603.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ01603. 1 interactor.
STRINGi7227.FBpp0088343.

Protein family/group databases

PeroxiBasei4118. DmPxt02-A.

Proteomic databases

PaxDbiQ01603.
PRIDEiQ01603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089287; FBpp0088343; FBgn0004577.
FBtr0301526; FBpp0290741; FBgn0004577.
GeneIDi2768671.
KEGGidme:Dmel_CG3477.
UCSCiCG3477-RA. d. melanogaster.

Organism-specific databases

CTDi2768671.
FlyBaseiFBgn0004577. Pxd.

Phylogenomic databases

eggNOGiKOG2408. Eukaryota.
ENOG410XPZ3. LUCA.
GeneTreeiENSGT00550000074325.
InParanoidiQ01603.
OMAiLHNMQWG.
OrthoDBiEOG091G00D8.
PhylomeDBiQ01603.

Enzyme and pathway databases

ReactomeiR-DME-209968. Thyroxine biosynthesis.
R-DME-6798695. Neutrophil degranulation.

Miscellaneous databases

GenomeRNAii2768671.
PROiQ01603.

Gene expression databases

BgeeiFBgn0004577.
ExpressionAtlasiQ01603. baseline.
GenevisibleiQ01603. DM.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR029585. Pxd_insect.
[Graphical view]
PANTHERiPTHR11475:SF65. PTHR11475:SF65. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS50292. PEROXIDASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPERO_DROME
AccessioniPrimary (citable) accession number: Q01603
Secondary accession number(s): Q6IJW8, Q7KSF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 2, 2010
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.