Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q01594

- ALLN1_ALLSA

UniProt

Q01594 - ALLN1_ALLSA

Protein

Alliin lyase 1

Gene
N/A
Organism
Allium sativum (Garlic)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Able to cleave the C-S bond of sulfoxide derivatives of Cys to produce allicin, thus giving rise to all sulfur compounds which are responsible for most of the properties of garlic, such as the specific smell and flavor as well as the health benefits like blood lipid or blood pressure lowering.

    Catalytic activityi

    An S-alkyl-L-cysteine S-oxide = an alkyl sulfenate + 2-aminoacrylate.

    Cofactori

    Pyridoxal phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei132 – 1321Chloride
    Binding sitei136 – 1361Chloride
    Binding sitei138 – 1381Chloride

    GO - Molecular functioni

    1. alliin lyase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Chloride, Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13504.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alliin lyase 1 (EC:4.4.1.4)
    Short name:
    Alliinase-1
    Alternative name(s):
    Cysteine sulphoxide lyase 1
    OrganismiAllium sativum (Garlic)
    Taxonomic identifieri4682 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesAmaryllidaceaeAllioideaeAllieaeAllium

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Propeptidei29 – 38101 PublicationPRO_0000020677
    Chaini39 – 486448Alliin lyase 1PRO_0000020678Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi58 ↔ 77
    Disulfide bondi79 ↔ 88
    Disulfide bondi82 ↔ 95
    Glycosylationi184 – 1841N-linked (GlcNAc...)
    Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
    Modified residuei289 – 2891N6-(pyridoxal phosphate)lysine
    Glycosylationi366 – 3661N-linked (GlcNAc...)
    Disulfide bondi406 ↔ 414

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Bulb and shoots.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1
    486
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi44 – 5411
    Helixi59 – 613
    Beta strandi83 – 853
    Helixi105 – 1073
    Helixi108 – 1114
    Helixi115 – 1184
    Beta strandi120 – 1223
    Turni124 – 1274
    Beta strandi129 – 1313
    Helixi141 – 15414
    Beta strandi163 – 1686
    Helixi169 – 18113
    Turni185 – 1873
    Beta strandi194 – 1985
    Helixi204 – 2129
    Beta strandi218 – 2236
    Helixi224 – 2274
    Helixi233 – 2353
    Beta strandi236 – 2438
    Turni245 – 2473
    Beta strandi259 – 2635
    Turni269 – 2713
    Beta strandi281 – 2866
    Helixi287 – 2904
    Helixi294 – 2963
    Beta strandi299 – 3035
    Helixi306 – 31914
    Beta strandi320 – 3223
    Helixi325 – 34521
    Helixi352 – 37120
    Beta strandi374 – 3785
    Beta strandi386 – 3883
    Turni389 – 3924
    Beta strandi393 – 3953
    Beta strandi400 – 4067
    Helixi409 – 4113
    Helixi414 – 4207
    Helixi428 – 4314
    Beta strandi437 – 4415
    Helixi446 – 46015

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LK9X-ray1.53A/B39-486[»]
    2HORX-ray1.60A39-465[»]
    2HOXX-ray1.40A/B/C/D39-465[»]
    ProteinModelPortaliQ01594.
    SMRiQ01594. Positions 39-465.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ01594.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 9747EGF-like; atypicalAdd
    BLAST

    Domaini

    The 6 Cys residues of the EGF-like domain are arranged in a disulfide pattern different from the one found in the canonical EGFs. The function of this domain is unclear. It may be a binding site for other proteins or the docking site for a putative alliinase receptor.

    Sequence similaritiesi

    Belongs to the alliinase family.Curated
    Contains 1 EGF-like domain.Curated

    Keywords - Domaini

    EGF-like domain, Signal

    Family and domain databases

    Gene3Di2.10.25.30. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR006948. Alliinase_C.
    IPR006947. EGF_alliinase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF04864. Alliinase_C. 1 hit.
    PF04863. EGF_alliinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01594-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVESYKKIGS CNKMPCLVIL TCIIMSNSLV NNNNMVQAKM TWTMKAAEEA    50
    EAVANINCSE HGRAFLDGII SEGSPKCECN TCYTGPDCSE KIQGCSADVA 100
    SGDGLFLEEY WKQHKEASAV LVSPWHRMSY FFNPVSNFIS FELEKTIKEL 150
    HEVVGNAAAK DRYIVFGVGV TQLIHGLVIS LSPNMTATPD APESKVVAHA 200
    PFYPVFREQT KYFNKKGYVW AGNAANYVNV SNPEQYIEMV TSPNNPEGLL 250
    RHAVIKGCKS IYDMVYYWPH YTPIKYKADE DILLFTMSKF TGHSGSRFGW 300
    ALIKDESVYN NLLNYMTKNT EGTPRETQLR SLKVLKEVVA MVKTQKGTMR 350
    DLNTFGFKKL RERWVNITAL LDQSDRFSYQ ELPQSEYCNY FRRMRPPSPS 400
    YAWVKCEWEE DKDCYQTFQN GRINTQNGVG FEASSRYVRL SLIKTQDDFD 450
    QLMYYLKDMV KAKRKTPLIK QLFIDQTETA SRRPFI 486
    Length:486
    Mass (Da):55,639
    Last modified:July 1, 1993 - v1
    Checksum:iD7862E867AD74383
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12622 mRNA. Translation: CAA78268.1.
    PIRiS29302.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    What's that smell? - Issue 39 of October 2003

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12622 mRNA. Translation: CAA78268.1 .
    PIRi S29302.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LK9 X-ray 1.53 A/B 39-486 [» ]
    2HOR X-ray 1.60 A 39-465 [» ]
    2HOX X-ray 1.40 A/B/C/D 39-465 [» ]
    ProteinModelPortali Q01594.
    SMRi Q01594. Positions 39-465.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13504.

    Miscellaneous databases

    EvolutionaryTracei Q01594.

    Family and domain databases

    Gene3Di 2.10.25.30. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR006948. Alliinase_C.
    IPR006947. EGF_alliinase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF04864. Alliinase_C. 1 hit.
    PF04863. EGF_alliinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of alliinase cDNA clones from garlic (Allium sativum L.) and related species."
      van Damme E.J.M., Smeets K., Torrekens S., van Leuven F., Peumans W.J.
      Eur. J. Biochem. 209:751-757(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-58.
      Tissue: Shoot.
    2. "Purification, characterization, and crystallization of alliinase from garlic."
      Kuettner E.B., Hilgenfeld R., Weiss M.S.
      Arch. Biochem. Biophys. 402:192-200(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, CRYSTALLIZATION.
      Tissue: Bulb.
    3. "The active principle of garlic at atomic resolution."
      Kuettner E.B., Hilgenfeld R., Weiss M.S.
      J. Biol. Chem. 277:46402-46407(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.53 ANGSTROMS) OF 39-465, SUBUNIT.
      Tissue: Bulb.

    Entry informationi

    Entry nameiALLN1_ALLSA
    AccessioniPrimary (citable) accession number: Q01594
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3