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Protein

B3 domain-containing transcription factor ABI3

Gene

ABI3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in abscisic acid-regulated gene expression during seed development. Regulates the transcription of SGR1 and SGR2 that are involved in leaf and embryo degreening.2 Publications

Miscellaneous

The truncated abi3-6 mutant lacking the DNA-binding domain is unable to localize to the nucleus and is an embryo stay-green mutant.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi572 – 674TF-B3PROSITE-ProRule annotationAdd BLAST103

GO - Molecular functioni

  • DNA binding Source: TAIR
  • transcription factor activity, RNA polymerase II transcription factor binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • embryo development ending in seed dormancy Source: TAIR
  • mitochondria-nucleus signaling pathway Source: TAIR
  • plastid organization Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to auxin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processAbscisic acid signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
B3 domain-containing transcription factor ABI3
Alternative name(s):
Protein ABSCISIC ACID-INSENSITIVE 3
Gene namesi
Name:ABI3
Ordered Locus Names:At3g24650
ORF Names:MSD24.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G24650.
TAIRilocus:2093166. AT3G24650.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001115041 – 720B3 domain-containing transcription factor ABI3Add BLAST720

Post-translational modificationi

Ubiquitinated by AIP2. Ubiquitination probably leads to its subsequent degradation, thus negatively regulating ABA signaling.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ01593.

Expressioni

Tissue specificityi

Isoform 2 accumulates only at the end of seed maturation.1 Publication

Developmental stagei

Expressed in the embryos, endosperm, and outer teguments of the seed throughout seed development.1 Publication

Inductioni

Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2.1 Publication

Gene expression databases

GenevisibleiQ01593. AT.

Interactioni

Subunit structurei

Interacts (via C-terminus) with SPK1, SCAR3, ABI5, APRR1, AIP2, AIP3 and AIP4. Binds to BZIP10 and BZIP25 and forms complexes made of ABI3, BZIP53 and BZIP25 or BZIP10.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi7392. 18 interactors.
IntActiQ01593. 8 interactors.
STRINGi3702.AT3G24650.1.

Structurei

3D structure databases

ProteinModelPortaliQ01593.
SMRiQ01593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410IJ7Z. Eukaryota.
ENOG4111P6C. LUCA.
InParanoidiQ01593.
OMAiEIWLDDH.
OrthoDBiEOG0936067A.
PhylomeDBiQ01593.

Family and domain databases

Gene3Di2.40.330.10. 1 hit.
InterProiView protein in InterPro
IPR003340. B3_DNA-bd.
IPR015300. DNA-bd_pseudobarrel.
PfamiView protein in Pfam
PF02362. B3. 1 hit.
SMARTiView protein in SMART
SM01019. B3. 1 hit.
SUPFAMiSSF101936. SSF101936. 1 hit.
PROSITEiView protein in PROSITE
PS50863. B3. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. Splicing is regulated by SUA.1 Publication
Isoform 1 (identifier: Q01593-1) [UniParc]FASTAAdd to basket
Also known as: ABI3-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSLHVAANA GDLAEDCGIL GGDADDTVLM DGIDEVGREI WLDDHGGDNN
60 70 80 90 100
HVHGHQDDDL IVHHDPSIFY GDLPTLPDFP CMSSSSSSST SPAPVNAIVS
110 120 130 140 150
SASSSSAASS STSSAASWAI LRSDGEDPTP NQNQYASGNC DDSSGALQST
160 170 180 190 200
ASMEIPLDSS QGFGCGEGGG DCIDMMETFG YMDLLDSNEF FDTSAIFSQD
210 220 230 240 250
DDTQNPNLMD QTLERQEDQV VVPMMENNSG GDMQMMNSSL EQDDDLAAVF
260 270 280 290 300
LEWLKNNKET VSAEDLRKVK IKKATIESAA RRLGGGKEAM KQLLKLILEW
310 320 330 340 350
VQTNHLQRRR TTTTTTNLSY QQSFQQDPFQ NPNPNNNNLI PPSDQTCFSP
360 370 380 390 400
STWVPPPPQQ QAFVSDPGFG YMPAPNYPPQ PEFLPLLESP PSWPPPPQSG
410 420 430 440 450
PMPHQQFPMP PTSQYNQFGD PTGFNGYNMN PYQYPYVPAG QMRDQRLLRL
460 470 480 490 500
CSSATKEARK KRMARQRRFL SHHHRHNNNN NNNNNNQQNQ TQIGETCAAV
510 520 530 540 550
APQLNPVATT ATGGTWMYWP NVPAVPPQLP PVMETQLPTM DRAGSASAMP
560 570 580 590 600
RQQVVPDRRQ GWKPEKNLRF LLQKVLKQSD VGNLGRIVLP KKEAETHLPE
610 620 630 640 650
LEARDGISLA MEDIGTSRVW NMRYRFWPNN KSRMYLLENT GDFVKTNGLQ
660 670 680 690 700
EGDFIVIYSD VKCGKYLIRG VKVRQPSGQK PEAPPSSAAT KRQNKSQRNI
710 720
NNNSPSANVV VASPTSQTVK
Length:720
Mass (Da):79,500
Last modified:October 1, 1994 - v1
Checksum:iD6A0F740D733060F
GO
Isoform 2 (identifier: Q01593-2) [UniParc]FASTAAdd to basket
Also known as: ABI3-beta

The sequence of this isoform differs from the canonical sequence as follows:
     415-429: YNQFGDPTGFNGYNM → TNERSEITPFVFLSN
     430-720: Missing.

Note: Due to a cryptic intron removal.1 Publication
Show »
Length:429
Mass (Da):46,669
Checksum:iFE9643CD51B878F4
GO

Sequence cautioni

The sequence BAB01214 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100S → P in BAD93896 (Ref. 4) Curated1
Sequence conflicti100S → P in BAD94272 (Ref. 4) Curated1
Sequence conflicti344D → V in BAD94272 (Ref. 4) Curated1
Sequence conflicti486Missing in CAA05484 (PubMed:1359917).Curated1
Sequence conflicti556P → L in BAE96106 (PubMed:16816409).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti225M → L in strain: cv. An-1, cv. Br-0, cv. Ct-1, cv. Cvi-1, cv. Edi-0, cv. Ga-0, cv. Kas-1, cv. Kin-0, cv. Landsberg erecta, cv. Ll-0, cv. Lz-0, cv. Ms-0, cv. Mt-0, cv. Nok-3, cv. Oy-0, cv. Se-0, cv. Sorbo, cv. Tsu-1, cv. Van-0, cv. Wa-1 and cv. Wassilewskija. 1 Publication1
Natural varianti231G → E in strain: cv. Kas-1 and cv. Sorbo. 1 Publication1
Natural varianti248A → T in strain: cv. Cvi-1. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058192415 – 429YNQFG…NGYNM → TNERSEITPFVFLSN in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_058193430 – 720Missing in isoform 2. 1 PublicationAdd BLAST291

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68141 mRNA. Translation: CAA48241.1.
AJ002473 Genomic DNA. Translation: CAA05484.1.
AP000740 Genomic DNA. Translation: BAB01214.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76933.1.
AK220739 mRNA. Translation: BAD93896.1.
AK220879 mRNA. Translation: BAD94272.1.
AB253328 Genomic DNA. Translation: BAE96106.1.
EF597723 Genomic DNA. Translation: ABR21503.1.
EF597724 Genomic DNA. Translation: ABR21504.1.
EF597725 Genomic DNA. Translation: ABR21505.1.
EF597726 Genomic DNA. Translation: ABR21506.1.
EF597727 Genomic DNA. Translation: ABR21507.1.
EF597728 Genomic DNA. Translation: ABR21508.1.
EF597729 Genomic DNA. Translation: ABR21509.1.
EF597730 Genomic DNA. Translation: ABR21510.1.
EF597731 Genomic DNA. Translation: ABR21511.1.
EF597732 Genomic DNA. Translation: ABR21512.1.
EF597733 Genomic DNA. Translation: ABR21513.1.
EF597734 Genomic DNA. Translation: ABR21514.1.
EF597735 Genomic DNA. Translation: ABR21515.1.
EF597736 Genomic DNA. Translation: ABR21516.1.
EF597737 Genomic DNA. Translation: ABR21517.1.
EF597738 Genomic DNA. Translation: ABR21518.1.
EF597739 Genomic DNA. Translation: ABR21519.1.
EF597740 Genomic DNA. Translation: ABR21520.1.
EF597741 Genomic DNA. Translation: ABR21521.1.
EF597743 Genomic DNA. Translation: ABR21523.1.
EF597744 Genomic DNA. Translation: ABR21524.1.
PIRiJQ1676.
RefSeqiNP_189108.1. NM_113376.4. [Q01593-1]
UniGeneiAt.19959.

Genome annotation databases

EnsemblPlantsiAT3G24650.1; AT3G24650.1; AT3G24650. [Q01593-1]
GeneIDi822061.
GrameneiAT3G24650.1; AT3G24650.1; AT3G24650.
KEGGiath:AT3G24650.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiABI3_ARATH
AccessioniPrimary (citable) accession number: Q01593
Secondary accession number(s): A6N2M3
, A6N2N0, A6N2P3, O23625, Q1EQV9, Q56ZT2, Q570G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: August 30, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names