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Protein

Integral membrane protein SED5

Gene

SED5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi complex. Acts as a target organelle soluble NSF attachment protein receptor (t-SNARE).

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: CACAO
  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: SGD
  • vesicle docking Source: GO_Central
  • vesicle fusion Source: SGD
  • vesicle fusion with Golgi apparatus Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32187-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.
R-SCE-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Integral membrane protein SED5
Gene namesi
Name:SED5
Ordered Locus Names:YLR026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR026C.
SGDiS000004016. SED5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 319CytoplasmicSequence analysisAdd BLAST319
Transmembranei320 – 340Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi206N → A: Loss of interaction with SEC23/SEC24 complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102731 – 340Integral membrane protein SED5Add BLAST340

Proteomic databases

MaxQBiQ01590.
PRIDEiQ01590.

PTM databases

iPTMnetiQ01590.

Interactioni

Subunit structurei

Interacts with SLY1, STF1, SFB3 and GOS1.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi31300. 470 interactors.
DIPiDIP-2489N.
IntActiQ01590. 24 interactors.
MINTiMINT-563409.
STRINGi4932.YLR026C.

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 4Combined sources4
Helixi7 – 20Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MQSX-ray3.00B1-45[»]
1PD0X-ray2.60B201-210[»]
ProteinModelPortaliQ01590.
SMRiQ01590.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01590.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini249 – 311t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili146 – 173Sequence analysisAdd BLAST28

Sequence similaritiesi

Belongs to the syntaxin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000000652.
HOGENOMiHOG000194860.
InParanoidiQ01590.
KOiK08490.
OMAiQDRTNEF.
OrthoDBiEOG092C42X0.

Family and domain databases

InterProiView protein in InterPro
IPR010989. SNARE.
IPR021538. Syntaxin-5_N.
IPR006012. Syntaxin/epimorphin_CS.
IPR000727. T_SNARE_dom.
PfamiView protein in Pfam
PF05739. SNARE. 1 hit.
PF11416. Syntaxin-5_N. 1 hit.
SMARTiView protein in SMART
SM00397. t_SNARE. 1 hit.
SUPFAMiSSF47661. SSF47661. 2 hits.
PROSITEiView protein in PROSITE
PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q01590-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKDRTSEF QQSVLSYKKR NKNFREQQRE RLQEKESENF ANNTTGNGKS
60 70 80 90 100
VSEFQKKASG IAHEISSTAQ LLSKLAVLAK RKPMFNDNPV EIAELSFLIK
110 120 130 140 150
RKIYAIEQSL VQLSQLKKTD VNGNTSNQSS KQPSAVQHSK NVVNLLNTQM
160 170 180 190 200
KNISGSFKDV LEERQRLEMA NKDRWQKLTT DTGHAPADDQ TQSNHAADLT
210 220 230 240 250
TYNNSNPFMT SLLDESSEKN NNSSNQGELS FPQNDSQLML MEEGQLSNNV
260 270 280 290 300
YLQERNRAVE TIESTIQEVG NLFQQLASMV QEQGEVIQRI DANVDDIDLN
310 320 330 340
ISGAQRELLK YFDRIKSNRW LAAKVFFIIF VFFVIWVLVN
Length:340
Mass (Da):38,807
Last modified:July 1, 1993 - v1
Checksum:iB3192B57269A0B83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66980 Genomic DNA. Translation: CAA47390.1.
Z73198 Genomic DNA. Translation: CAA97549.1.
BK006945 Genomic DNA. Translation: DAA09344.1.
PIRiA44019.
RefSeqiNP_013126.1. NM_001181913.1.

Genome annotation databases

EnsemblFungiiYLR026C; YLR026C; YLR026C.
GeneIDi850713.
KEGGisce:YLR026C.

Similar proteinsi

Entry informationi

Entry nameiSED5_YEAST
AccessioniPrimary (citable) accession number: Q01590
Secondary accession number(s): D6VY28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 27, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names