Integral membrane protein SED5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q01590 (SED5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Integral membrane protein SED5

Gene names

Name:	SED5
Ordered Locus Names:	YLR026C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vesicular transport between the endoplasmic reticulum and the Golgi complex. Acts as a target organelle soluble NSF attachment protein receptor (t-SNARE).
Subunit structure	Interacts with SLY1, STF1, SFB3 and GOS1. Ref.4 Ref.5
Subcellular location	Membrane; Single-pass type IV membrane protein Potential. Golgi apparatus membrane; Single-pass type IV membrane protein Potential.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype. Source: SGD intra-Golgi vesicle-mediated transport Inferred from mutant phenotype. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle fusion with Golgi apparatus Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Inferred from direct assay. Source: SGD cis-Golgi network Inferred from direct assay. Source: SGD integral to membrane Inferred from direct assay Ref.1. Source: SGD
Molecular function	SNAP receptor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
COG2	P53271	2	EBI-16930,EBI-16614
COG3	P40094	4	EBI-16930,EBI-16605
GOS1	P38736	4	EBI-16930,EBI-24365
SLY1	P22213	3	EBI-16930,EBI-17387

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 340

340

Integral membrane protein SED5

PRO_0000210273

Regions

Topological domain

1 – 319

319

Cytoplasmic Potential

Transmembrane

320 – 340

Helical; Anchor for type IV membrane protein; Potential

Domain

249 – 311

t-SNARE coiled-coil homology

Coiled coil

146 – 173

Potential

Experimental info

Mutagenesis

206

N → A: Loss of interaction with SEC23/SEC24 complex. Ref.5

Secondary structure

340

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q01590 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: B3192B57269A0B83
Show »

FASTA

340

38,807

        10         20         30         40         50         60 
MNIKDRTSEF QQSVLSYKKR NKNFREQQRE RLQEKESENF ANNTTGNGKS VSEFQKKASG 

        70         80         90        100        110        120 
IAHEISSTAQ LLSKLAVLAK RKPMFNDNPV EIAELSFLIK RKIYAIEQSL VQLSQLKKTD 

       130        140        150        160        170        180 
VNGNTSNQSS KQPSAVQHSK NVVNLLNTQM KNISGSFKDV LEERQRLEMA NKDRWQKLTT 

       190        200        210        220        230        240 
DTGHAPADDQ TQSNHAADLT TYNNSNPFMT SLLDESSEKN NNSSNQGELS FPQNDSQLML 

       250        260        270        280        290        300 
MEEGQLSNNV YLQERNRAVE TIESTIQEVG NLFQQLASMV QEQGEVIQRI DANVDDIDLN 

       310        320        330        340 
ISGAQRELLK YFDRIKSNRW LAAKVFFIIF VFFVIWVLVN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex." Hardwick K.G., Pelham H.R.B. J. Cell Biol. 119:513-521(1992) [PubMed: 1400588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Gos1p, a Saccharomyces cerevisiae SNARE protein involved in Golgi transport." McNew J.A., Coe J.G.S., Sogaard M., Zemelman B.V., Wimmer C., Hong W., Soellner T.H. FEBS Lett. 435:89-95(1998) [PubMed: 9755865] [Abstract] Cited for: INTERACTION WITH GOS1.
[5]	"SNARE selectivity of the COPII coat." Mossessova E., Bickford L.C., Goldberg J. Cell 114:483-495(2003) [PubMed: 12941276] [Abstract] Cited for: INTERACTION WITH SEC24, MUTAGENESIS OF ASN-206.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p." Bracher A., Weissenhorn W. EMBO J. 21:6114-6124(2002) [PubMed: 12426383] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-45 IN COMPLEX WITH SLY1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X66980 Genomic DNA. Translation: CAA47390.1.
Z73198 Genomic DNA. Translation: CAA97549.1.
BK006945 Genomic DNA. Translation: DAA09344.1.

PIR

A44019.

RefSeq

NP_013126.1. NM_001181913.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MQS	X-ray	3.00	B	1-45	[»]
1PD0	X-ray	2.60	B	201-210	[»]

ProteinModelPortal

Q01590.

SMR

Q01590. Positions 254-319.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2489N.

IntAct

Q01590. 14 interactions.

MINT

MINT-563409.

STRING

Q01590.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLR026C; YLR026C; YLR026C.

GeneID

850713.

KEGG

sce:YLR026C.

NMPDR

fig|4932.3.peg.4117.

Organism-specific databases

CYGD

YLR026c.

SGD

S000004016. SED5.

Phylogenomic databases

eggNOG

fuNOG07432.

GeneTree

EFGT00050000002423.

HOGENOM

HBG628558.

OMA

AESHASK.

OrthoDB

EOG49PF76.

Gene expression databases

ArrayExpress

Q01590.

Genevestigator

Q01590.

GermOnline

YLR026C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR021538. Integral_membrane_Sed5p.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]

K08490.

Pfam

PF11416. Sed5p. 1 hit.
PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

PROSITE

PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

966775.

Entry information

Entry name

SED5_YEAST

Accession

Primary (citable) accession number: Q01590
Secondary accession number(s): D6VY28

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	December 14, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents