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Protein

Integral membrane protein SED5

Gene

SED5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi complex. Acts as a target organelle soluble NSF attachment protein receptor (t-SNARE).

GO - Molecular functioni

  • SNAP receptor activity Source: SGD

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: CACAO
  • intracellular protein transport Source: InterPro
  • intra-Golgi vesicle-mediated transport Source: SGD
  • vesicle fusion Source: SGD
  • vesicle fusion with Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32187-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.
R-SCE-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Integral membrane protein SED5
Gene namesi
Name:SED5
Ordered Locus Names:YLR026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR026C.
SGDiS000004016. SED5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 319319CytoplasmicSequence analysisAdd
BLAST
Transmembranei320 – 34021Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST

GO - Cellular componenti

  • cis-Golgi network Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
  • SNARE complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi206 – 2061N → A: Loss of interaction with SEC23/SEC24 complex. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Integral membrane protein SED5PRO_0000210273Add
BLAST

Proteomic databases

MaxQBiQ01590.

PTM databases

iPTMnetiQ01590.

Interactioni

Subunit structurei

Interacts with SLY1, STF1, SFB3 and GOS1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COG2P532712EBI-16930,EBI-16614
COG3P400944EBI-16930,EBI-16605
GOS1P387365EBI-16930,EBI-24365
SEC22P222143EBI-16930,EBI-16577
SLY1P222133EBI-16930,EBI-17387

GO - Molecular functioni

  • SNAP receptor activity Source: SGD

Protein-protein interaction databases

BioGridi31300. 104 interactions.
DIPiDIP-2489N.
IntActiQ01590. 15 interactions.
MINTiMINT-563409.

Structurei

Secondary structure

340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 44Combined sources
Helixi7 – 2014Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MQSX-ray3.00B1-45[»]
1PD0X-ray2.60B201-210[»]
ProteinModelPortaliQ01590.
SMRiQ01590. Positions 258-295.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01590.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini249 – 31163t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili146 – 17328Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000000652.
HOGENOMiHOG000194860.
InParanoidiQ01590.
KOiK08490.
OMAiMEEAQTN.
OrthoDBiEOG7G1VHK.

Family and domain databases

InterProiIPR021538. Syntaxin-5_N.
IPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF11416. Syntaxin-5_N. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 2 hits.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01590-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKDRTSEF QQSVLSYKKR NKNFREQQRE RLQEKESENF ANNTTGNGKS
60 70 80 90 100
VSEFQKKASG IAHEISSTAQ LLSKLAVLAK RKPMFNDNPV EIAELSFLIK
110 120 130 140 150
RKIYAIEQSL VQLSQLKKTD VNGNTSNQSS KQPSAVQHSK NVVNLLNTQM
160 170 180 190 200
KNISGSFKDV LEERQRLEMA NKDRWQKLTT DTGHAPADDQ TQSNHAADLT
210 220 230 240 250
TYNNSNPFMT SLLDESSEKN NNSSNQGELS FPQNDSQLML MEEGQLSNNV
260 270 280 290 300
YLQERNRAVE TIESTIQEVG NLFQQLASMV QEQGEVIQRI DANVDDIDLN
310 320 330 340
ISGAQRELLK YFDRIKSNRW LAAKVFFIIF VFFVIWVLVN
Length:340
Mass (Da):38,807
Last modified:July 1, 1993 - v1
Checksum:iB3192B57269A0B83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66980 Genomic DNA. Translation: CAA47390.1.
Z73198 Genomic DNA. Translation: CAA97549.1.
BK006945 Genomic DNA. Translation: DAA09344.1.
PIRiA44019.
RefSeqiNP_013126.1. NM_001181913.1.

Genome annotation databases

EnsemblFungiiYLR026C; YLR026C; YLR026C.
GeneIDi850713.
KEGGisce:YLR026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66980 Genomic DNA. Translation: CAA47390.1.
Z73198 Genomic DNA. Translation: CAA97549.1.
BK006945 Genomic DNA. Translation: DAA09344.1.
PIRiA44019.
RefSeqiNP_013126.1. NM_001181913.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MQSX-ray3.00B1-45[»]
1PD0X-ray2.60B201-210[»]
ProteinModelPortaliQ01590.
SMRiQ01590. Positions 258-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31300. 104 interactions.
DIPiDIP-2489N.
IntActiQ01590. 15 interactions.
MINTiMINT-563409.

PTM databases

iPTMnetiQ01590.

Proteomic databases

MaxQBiQ01590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR026C; YLR026C; YLR026C.
GeneIDi850713.
KEGGisce:YLR026C.

Organism-specific databases

EuPathDBiFungiDB:YLR026C.
SGDiS000004016. SED5.

Phylogenomic databases

GeneTreeiENSGT00390000000652.
HOGENOMiHOG000194860.
InParanoidiQ01590.
KOiK08490.
OMAiMEEAQTN.
OrthoDBiEOG7G1VHK.

Enzyme and pathway databases

BioCyciYEAST:G3O-32187-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.
R-SCE-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiQ01590.
PROiQ01590.

Family and domain databases

InterProiIPR021538. Syntaxin-5_N.
IPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF11416. Syntaxin-5_N. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 2 hits.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex."
    Hardwick K.G., Pelham H.R.B.
    J. Cell Biol. 119:513-521(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Gos1p, a Saccharomyces cerevisiae SNARE protein involved in Golgi transport."
    McNew J.A., Coe J.G.S., Sogaard M., Zemelman B.V., Wimmer C., Hong W., Soellner T.H.
    FEBS Lett. 435:89-95(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOS1.
  5. Cited for: INTERACTION WITH SEC24, MUTAGENESIS OF ASN-206.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p."
    Bracher A., Weissenhorn W.
    EMBO J. 21:6114-6124(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-45 IN COMPLEX WITH SLY1.

Entry informationi

Entry nameiSED5_YEAST
AccessioniPrimary (citable) accession number: Q01590
Secondary accession number(s): D6VY28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.