Reviewed, UniProtKB/Swiss-Prot Q01590 (SED5_YEAST)

Names and origin

Protein names

Recommended name:
    Integral membrane protein SED5

Gene names

Name:	SED5
Ordered Locus Names:	YLR026C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for vesicular transport between the endoplasmic reticulum and the Golgi complex. Acts as a target organelle soluble NSF attachment protein receptor (t-SNARE).
Subunit structure	Interacts with SLY1, STF1, SFB3 and GOS1. Ref.3 Ref.4
Subcellular location	Membrane; Single-pass type IV membrane protein Potential. Golgi apparatus membrane; Single-pass type IV membrane protein Potential.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Transmembrane
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Traceable author statement. Source: SGD intra-Golgi vesicle-mediated transport Traceable author statement. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde vesicle-mediated transport, Golgi to ER Traceable author statement. Source: SGD vesicle fusion Traceable author statement. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cis-Golgi network Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	SNAP receptor activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COG2	P53271	1	EBI-16930,EBI-16614
COG3	P40094	1	EBI-16930,EBI-16605
GET3	Q12154	2	EBI-16930,EBI-2989
GOS1	P38736	1	EBI-16930,EBI-24365
SLY1	P22213	2	EBI-16930,EBI-17387

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 340

340

Integral membrane protein SED5

PRO_0000210273

Regions

Topological domain

1 – 319

319

Cytoplasmic Potential

Transmembrane

320 – 340

21

Anchor for type IV membrane protein Potential

Domain

249 – 311

63

t-SNARE coiled-coil homology

Coiled coil

146 – 173

28

Potential

Experimental info

Mutagenesis

206

1

N → A: Loss of interaction with SEC23/SEC24 complex. Ref.4

Secondary structure

1

.

.

.

.

340

Helix Strand Turn

Details...

Helix

1 – 4

4

Helix

7 – 20

14

Sequences

	Sequence		Length	Mass (Da)
	Q01590-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: B3192B57269A0B83	FASTA	340	38,807
	10 20 30 40 50 60 MNIKDRTSEF QQSVLSYKKR NKNFREQQRE RLQEKESENF ANNTTGNGKS VSEFQKKASG 70 80 90 100 110 120 IAHEISSTAQ LLSKLAVLAK RKPMFNDNPV EIAELSFLIK RKIYAIEQSL VQLSQLKKTD 130 140 150 160 170 180 VNGNTSNQSS KQPSAVQHSK NVVNLLNTQM KNISGSFKDV LEERQRLEMA NKDRWQKLTT 190 200 210 220 230 240 DTGHAPADDQ TQSNHAADLT TYNNSNPFMT SLLDESSEKN NNSSNQGELS FPQNDSQLML 250 260 270 280 290 300 MEEGQLSNNV YLQERNRAVE TIESTIQEVG NLFQQLASMV QEQGEVIQRI DANVDDIDLN 310 320 330 340 ISGAQRELLK YFDRIKSNRW LAAKVFFIIF VFFVIWVLVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex." Hardwick K.G., Pelham H.R.B. J. Cell Biol. 119:513-521(1992) [PubMed: 1400588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Gos1p, a Saccharomyces cerevisiae SNARE protein involved in Golgi transport." McNew J.A., Coe J.G.S., Sogaard M., Zemelman B.V., Wimmer C., Hong W., Soellner T.H. FEBS Lett. 435:89-95(1998) [PubMed: 9755865] [Abstract] Cited for: INTERACTION WITH GOS1.
[4]	"SNARE selectivity of the COPII coat." Mossessova E., Bickford L.C., Goldberg J. Cell 114:483-495(2003) [PubMed: 12941276] [Abstract] Cited for: INTERACTION WITH SEC24, MUTAGENESIS OF ASN-206.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p." Bracher A., Weissenhorn W. EMBO J. 21:6114-6124(2002) [PubMed: 12426383] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-45 IN COMPLEX WITH SLY1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X66980 Genomic DNA. Translation: CAA47390.1.
Z73198 Genomic DNA. Translation: CAA97549.1.

PIR

A44019.

RefSeq

NP_013126.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MQS	X-ray	3.00	B	1-45	[»]
1PD0	X-ray	2.60	B	201-210	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2489N.

IntAct

Q01590. 9 interactions.

Genome annotation databases

Ensembl

YLR026C. Saccharomyces cerevisiae. [Contig view]

GeneID

850713.

GenomeReviews

Gene locus YLR026C in contig Y13138_GR.

KEGG

sce:YLR026C.

NMPDR

fig|4932.3.peg.4117.

Organism-specific databases

CYGD

YLR026c.

SGD

S000004016. SED5.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q01590.

OMA

Q01590. QRIDENV.

Gene expression databases

GermOnline

YLR026C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR000727. T_SNARE.
[Graphical view]

Pfam

PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

PROSITE

PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

966775.

Entry information

Entry name

SED5_YEAST

Accession

Primary (citable) accession number: Q01590

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)