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Q01583

- DGK1_DROME

UniProt

Q01583 - DGK1_DROME

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Protein

Diacylglycerol kinase 1

Gene

Dgk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity (By similarity). May have a role in the development of the embryonic nervous system and the function of the adult nervous system and muscle; regulating signal transduction in neurons.By similarity2 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi299 – 310121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi344 – 355122PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri382 – 43251Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri449 – 49850Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. diacylglycerol kinase activity Source: FlyBase
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. phosphorylation Source: FlyBase
  3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_235986. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 1 (EC:2.7.1.107)
Short name:
DAG kinase 1
Short name:
DGK 1
Short name:
Diglyceride kinase 1
Gene namesi
Name:Dgk
Synonyms:DGK1
ORF Names:CG34361
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0085390. Dgk.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12111211Diacylglycerol kinase 1PRO_0000218473Add
BLAST

Proteomic databases

PaxDbiQ01583.

Expressioni

Tissue specificityi

In 10-11 hours embryos, expression is abundant in a limited number of cells in the procephalic region and in the ventral nerve cord. Predominantly expressed in the adult nervous system and muscle: including compound eyes, brain cortex, fibrillar muscle, and tubular muscle.2 Publications

Developmental stagei

Expressed in the embryonic, pupal and adult stages, with little expression during the larval stages.2 Publications

Gene expression databases

BgeeiQ01583.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ01583.
SMRiQ01583. Positions 13-85, 270-370, 383-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 32136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini331 – 36636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini548 – 682135DAGKcPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi731 – 79262Thr-richAdd
BLAST
Compositional biasi850 – 973124Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri382 – 43251Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri449 – 49850Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
InParanoidiQ01583.
KOiK00901.
OrthoDBiEOG75XGK8.
PhylomeDBiQ01583.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform E (identifier: Q01583-4) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNIGIAAPKW DKLSPREFLQ LQELASYSTR KLQDVLREFS SPSAASTPKC
60 70 80 90 100
IPDGDIDFDG FRRFLDAFLD CEAPLDLAKH LFVSFLKPNV TQAQLHGRAL
110 120 130 140 150
NQMAAISSTA ACAPVTSHTK GSIPNINSIA ELMPQCSGGG GGIGGTGGVA
160 170 180 190 200
GAEGHAQARS SFVDKIHGIT DKLHHSLGGH LSHDPSKTGS VHPMLTVTPS
210 220 230 240 250
PLASGPSMFQ ASNPARRSVD SSPSHSATNH SQMSRNSSKK SSNSVNCKID
260 270 280 290 300
ADIKLLARKL SHFDPLTLKV PLKDVVCYLS LLEAGRPEDK LEFMFRLYDT
310 320 330 340 350
DSNGVLDTAE MDAIVNQMMA VAEYLGWDVS ELRPILQEMM VEIDYDADGT
360 370 380 390 400
VSLDEWQRGG MTTIPLLVLL GVDSTTLKED GIHVWRLKHF SKPAYCNLCL
410 420 430 440 450
NMLVGLGKKG LCCVLCKYTV HERCVQHAPA SCITTYVKSK KPKCGGDLLH
460 470 480 490 500
HWVEGNCYGR CSKCRKRIKA YHGITGLTCR WCHMMLHNRC ASSVKKECTL
510 520 530 540 550
GEYSELIVPP TAICPAVLDR QRSVNQAHKK SQMHHHQATH FQITPPDELS
560 570 580 590 600
CPLLVFVNPK SGGRQGDRIL RKFQYMLNPR QVYDLSKGGP KEGLTLFKDL
610 620 630 640 650
PRFRVICCGG DGTVGWVLEA MDSIELASQP AIGVIPLGTG NDLARCLRWG
660 670 680 690 700
GGYEGENIPK LMDKFRRAST VMLDRWSIEV TNTPHSDDMR PKVTLHSNMQ
710 720 730 740 750
KVIELSQSVV VDKSLMERFE EIQRQSKQVA TSMGTAASST SIMMASKTET
760 770 780 790 800
EMETMATMEF GSSTTTTNRT TTTKSISMST FETQCLQQTL RTAMSSSSSN
810 820 830 840 850
TSSGSPCNGN QDAETEVDSH AAAAADVREK SVPRRSGETE KQSLETLLLQ
860 870 880 890 900
HKQQMQQQQQ QQQQGVTSLA VEEAATATPV GSNQSDNSSQ RNKQNNILKQ
910 920 930 940 950
QITLSLDLSD HEDEPKDDGG GAGDGTKSNG NSIPATPATP ITPTTPNAAS
960 970 980 990 1000
SVLQQQQQQH LQFEQQQKPI KVQSDKDCTV PYNIINNYFS VGVDAAICVK
1010 1020 1030 1040 1050
FHLEREKNPH KFNSRMKNKL WYFEYATSET FAASCKNLHE SIEIVCDGVA
1060 1070 1080 1090 1100
LDLANGPHLQ GVALLNIPYT HGGSNLWGEH LSQKRIRKSA GPFGKSKKLR
1110 1120 1130 1140 1150
AGDKEFSATS FNSVDLSVAI QDFGDRLIEV IGLENCLHMG QVRTGLRASG
1160 1170 1180 1190 1200
RRLAQCSEVI IKTKKTFPMQ IDGEPWMQMP CTIKVTHKNQ VPMLMAPRSE
1210
KGRGFFNLLC S

Note: No experimental confirmation available.

Length:1,211
Mass (Da):132,960
Last modified:April 29, 2008 - v5
Checksum:iCC58412EE1E8F762
GO
Isoform C (identifier: Q01583-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-698: Missing.

Show »
Length:513
Mass (Da):56,324
Checksum:iBA50553F32F19602
GO
Isoform F (identifier: Q01583-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-250: GSIPNINSIA...SSNSVNCKID → AVSRILPAIGRHCPGSLESGSAQAEKR

Note: No experimental confirmation available.

Show »
Length:1,108
Mass (Da):122,602
Checksum:i78CF327DBEDDF6ED
GO
Isoform G (identifier: Q01583-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-73: NIGIAAPKWD...FLDAFLDCEA → RWELRYGWQI...AGCCCIGKYF
     74-485: Missing.
     530-537: Missing.

Show »
Length:791
Mass (Da):87,297
Checksum:i9DFD00E280FD64A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti794 – 7941M → T in BAA01894. (PubMed:1321433)Curated
Sequence conflicti863 – 8631Q → QQQQQ in CAA47750. (PubMed:8380995)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 698698Missing in isoform C. 1 PublicationVSP_017884Add
BLAST
Alternative sequencei2 – 7372NIGIA…LDCEA → RWELRYGWQIVTFRTNCATA AAVACHQLLCPVSLTSAVEI MPNSFPPEGRRRERRSGNLV LRAGCCCIGKYF in isoform G. 2 PublicationsVSP_017885Add
BLAST
Alternative sequencei74 – 485412Missing in isoform G. 2 PublicationsVSP_017886Add
BLAST
Alternative sequencei121 – 250130GSIPN…NCKID → AVSRILPAIGRHCPGSLESG SAQAEKR in isoform F. CuratedVSP_033247Add
BLAST
Alternative sequencei530 – 5378Missing in isoform G. 2 PublicationsVSP_017887

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11120 mRNA. Translation: BAA01894.1.
X67335 mRNA. Translation: CAA47750.1.
AE013599 Genomic DNA. Translation: AAF59180.1.
AE013599 Genomic DNA. Translation: AAS64897.1.
AE013599 Genomic DNA. Translation: ABV53719.1.
AE013599 Genomic DNA. Translation: ABV53720.1.
BT003323 mRNA. Translation: AAO25083.1.
PIRiA46140.
B46140.
RefSeqiNP_001097219.1. NM_001103749.2. [Q01583-4]
NP_001097220.1. NM_001103750.3. [Q01583-1]
NP_523654.2. NM_078930.4. [Q01583-2]
NP_995775.2. NM_206053.2.
UniGeneiDm.3102.

Genome annotation databases

EnsemblMetazoaiFBtr0112580; FBpp0111492; FBgn0085390. [Q01583-4]
GeneIDi35738.
KEGGidme:Dmel_CG34361.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11120 mRNA. Translation: BAA01894.1 .
X67335 mRNA. Translation: CAA47750.1 .
AE013599 Genomic DNA. Translation: AAF59180.1 .
AE013599 Genomic DNA. Translation: AAS64897.1 .
AE013599 Genomic DNA. Translation: ABV53719.1 .
AE013599 Genomic DNA. Translation: ABV53720.1 .
BT003323 mRNA. Translation: AAO25083.1 .
PIRi A46140.
B46140.
RefSeqi NP_001097219.1. NM_001103749.2. [Q01583-4 ]
NP_001097220.1. NM_001103750.3. [Q01583-1 ]
NP_523654.2. NM_078930.4. [Q01583-2 ]
NP_995775.2. NM_206053.2.
UniGenei Dm.3102.

3D structure databases

ProteinModelPortali Q01583.
SMRi Q01583. Positions 13-85, 270-370, 383-432.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q01583.
ChEMBLi CHEMBL1075068.

Proteomic databases

PaxDbi Q01583.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0112580 ; FBpp0111492 ; FBgn0085390 . [Q01583-4 ]
GeneIDi 35738.
KEGGi dme:Dmel_CG34361.

Organism-specific databases

CTDi 35738.
FlyBasei FBgn0085390. Dgk.

Phylogenomic databases

eggNOGi NOG47311.
GeneTreei ENSGT00760000119050.
InParanoidi Q01583.
KOi K00901.
OrthoDBi EOG75XGK8.
PhylomeDBi Q01583.

Enzyme and pathway databases

Reactomei REACT_235986. Effects of PIP2 hydrolysis.

Miscellaneous databases

ChiTaRSi rdgA. fly.
GenomeRNAii 35738.
NextBioi 794970.

Gene expression databases

Bgeei Q01583.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 3 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a Drosophila diacylglycerol kinase gene that is expressed in the nervous system and muscle."
    Masai I., Hosoya T., Kojima S., Hotta Y.
    Proc. Natl. Acad. Sci. U.S.A. 89:6030-6034(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM G), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Oregon-R.
  2. "A Drosophila gene encoding a protein with similarity to diacylglycerol kinase is expressed in specific neurons."
    Harden N., Yap S.F., Chiam M.-A., Lim L.
    Biochem. J. 289:439-444(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Canton-S.
    Tissue: Embryo.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM G).
    Strain: Berkeley.
    Tissue: Testis.

Entry informationi

Entry nameiDGK1_DROME
AccessioniPrimary (citable) accession number: Q01583
Secondary accession number(s): A8DY60
, A8DY61, Q53YF9, Q7KQR7, Q8MKP0, Q9V327
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 29, 2008
Last modified: November 26, 2014
This is version 125 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3