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Q01583

- DGK1_DROME

UniProt

Q01583 - DGK1_DROME

Protein

Diacylglycerol kinase 1

Gene

Dgk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 5 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity By similarity. May have a role in the development of the embryonic nervous system and the function of the adult nervous system and muscle; regulating signal transduction in neurons.By similarity2 Publications

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi299 – 310121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi344 – 355122PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri382 – 43251Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri449 – 49850Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: FlyBase
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. phosphorylation Source: FlyBase
    3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase 1 (EC:2.7.1.107)
    Short name:
    DAG kinase 1
    Short name:
    DGK 1
    Short name:
    Diglyceride kinase 1
    Gene namesi
    Name:Dgk
    Synonyms:DGK1
    ORF Names:CG34361
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0085390. Dgk.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12111211Diacylglycerol kinase 1PRO_0000218473Add
    BLAST

    Proteomic databases

    PaxDbiQ01583.

    Expressioni

    Tissue specificityi

    In 10-11 hours embryos, expression is abundant in a limited number of cells in the procephalic region and in the ventral nerve cord. Predominantly expressed in the adult nervous system and muscle: including compound eyes, brain cortex, fibrillar muscle, and tubular muscle.2 Publications

    Developmental stagei

    Expressed in the embryonic, pupal and adult stages, with little expression during the larval stages.2 Publications

    Gene expression databases

    BgeeiQ01583.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01583.
    SMRiQ01583. Positions 13-85, 284-358, 383-432.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini286 – 32136EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini331 – 36636EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini548 – 682135DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi731 – 79262Thr-richAdd
    BLAST
    Compositional biasi850 – 973124Gln-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri382 – 43251Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri449 – 49850Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00750000117569.
    InParanoidiQ01583.
    KOiK00901.
    OrthoDBiEOG75XGK8.
    PhylomeDBiQ01583.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 2 hits.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 3 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform E (identifier: Q01583-4) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNIGIAAPKW DKLSPREFLQ LQELASYSTR KLQDVLREFS SPSAASTPKC     50
    IPDGDIDFDG FRRFLDAFLD CEAPLDLAKH LFVSFLKPNV TQAQLHGRAL 100
    NQMAAISSTA ACAPVTSHTK GSIPNINSIA ELMPQCSGGG GGIGGTGGVA 150
    GAEGHAQARS SFVDKIHGIT DKLHHSLGGH LSHDPSKTGS VHPMLTVTPS 200
    PLASGPSMFQ ASNPARRSVD SSPSHSATNH SQMSRNSSKK SSNSVNCKID 250
    ADIKLLARKL SHFDPLTLKV PLKDVVCYLS LLEAGRPEDK LEFMFRLYDT 300
    DSNGVLDTAE MDAIVNQMMA VAEYLGWDVS ELRPILQEMM VEIDYDADGT 350
    VSLDEWQRGG MTTIPLLVLL GVDSTTLKED GIHVWRLKHF SKPAYCNLCL 400
    NMLVGLGKKG LCCVLCKYTV HERCVQHAPA SCITTYVKSK KPKCGGDLLH 450
    HWVEGNCYGR CSKCRKRIKA YHGITGLTCR WCHMMLHNRC ASSVKKECTL 500
    GEYSELIVPP TAICPAVLDR QRSVNQAHKK SQMHHHQATH FQITPPDELS 550
    CPLLVFVNPK SGGRQGDRIL RKFQYMLNPR QVYDLSKGGP KEGLTLFKDL 600
    PRFRVICCGG DGTVGWVLEA MDSIELASQP AIGVIPLGTG NDLARCLRWG 650
    GGYEGENIPK LMDKFRRAST VMLDRWSIEV TNTPHSDDMR PKVTLHSNMQ 700
    KVIELSQSVV VDKSLMERFE EIQRQSKQVA TSMGTAASST SIMMASKTET 750
    EMETMATMEF GSSTTTTNRT TTTKSISMST FETQCLQQTL RTAMSSSSSN 800
    TSSGSPCNGN QDAETEVDSH AAAAADVREK SVPRRSGETE KQSLETLLLQ 850
    HKQQMQQQQQ QQQQGVTSLA VEEAATATPV GSNQSDNSSQ RNKQNNILKQ 900
    QITLSLDLSD HEDEPKDDGG GAGDGTKSNG NSIPATPATP ITPTTPNAAS 950
    SVLQQQQQQH LQFEQQQKPI KVQSDKDCTV PYNIINNYFS VGVDAAICVK 1000
    FHLEREKNPH KFNSRMKNKL WYFEYATSET FAASCKNLHE SIEIVCDGVA 1050
    LDLANGPHLQ GVALLNIPYT HGGSNLWGEH LSQKRIRKSA GPFGKSKKLR 1100
    AGDKEFSATS FNSVDLSVAI QDFGDRLIEV IGLENCLHMG QVRTGLRASG 1150
    RRLAQCSEVI IKTKKTFPMQ IDGEPWMQMP CTIKVTHKNQ VPMLMAPRSE 1200
    KGRGFFNLLC S 1211

    Note: No experimental confirmation available.

    Length:1,211
    Mass (Da):132,960
    Last modified:April 29, 2008 - v5
    Checksum:iCC58412EE1E8F762
    GO
    Isoform C (identifier: Q01583-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-698: Missing.

    Show »
    Length:513
    Mass (Da):56,324
    Checksum:iBA50553F32F19602
    GO
    Isoform F (identifier: Q01583-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         121-250: GSIPNINSIA...SSNSVNCKID → AVSRILPAIGRHCPGSLESGSAQAEKR

    Note: No experimental confirmation available.

    Show »
    Length:1,108
    Mass (Da):122,602
    Checksum:i78CF327DBEDDF6ED
    GO
    Isoform G (identifier: Q01583-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-73: NIGIAAPKWD...FLDAFLDCEA → RWELRYGWQI...AGCCCIGKYF
         74-485: Missing.
         530-537: Missing.

    Show »
    Length:791
    Mass (Da):87,297
    Checksum:i9DFD00E280FD64A6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti794 – 7941M → T in BAA01894. (PubMed:1321433)Curated
    Sequence conflicti863 – 8631Q → QQQQQ in CAA47750. (PubMed:8380995)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 698698Missing in isoform C. 1 PublicationVSP_017884Add
    BLAST
    Alternative sequencei2 – 7372NIGIA…LDCEA → RWELRYGWQIVTFRTNCATA AAVACHQLLCPVSLTSAVEI MPNSFPPEGRRRERRSGNLV LRAGCCCIGKYF in isoform G. 2 PublicationsVSP_017885Add
    BLAST
    Alternative sequencei74 – 485412Missing in isoform G. 2 PublicationsVSP_017886Add
    BLAST
    Alternative sequencei121 – 250130GSIPN…NCKID → AVSRILPAIGRHCPGSLESG SAQAEKR in isoform F. CuratedVSP_033247Add
    BLAST
    Alternative sequencei530 – 5378Missing in isoform G. 2 PublicationsVSP_017887

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11120 mRNA. Translation: BAA01894.1.
    X67335 mRNA. Translation: CAA47750.1.
    AE013599 Genomic DNA. Translation: AAF59180.1.
    AE013599 Genomic DNA. Translation: AAS64897.1.
    AE013599 Genomic DNA. Translation: ABV53719.1.
    AE013599 Genomic DNA. Translation: ABV53720.1.
    BT003323 mRNA. Translation: AAO25083.1.
    PIRiA46140.
    B46140.
    RefSeqiNP_001097219.1. NM_001103749.2. [Q01583-4]
    NP_001097220.1. NM_001103750.3. [Q01583-1]
    NP_523654.2. NM_078930.4. [Q01583-2]
    NP_995775.2. NM_206053.2.
    UniGeneiDm.3102.

    Genome annotation databases

    EnsemblMetazoaiFBtr0112580; FBpp0111492; FBgn0085390. [Q01583-4]
    GeneIDi35738.
    KEGGidme:Dmel_CG34361.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11120 mRNA. Translation: BAA01894.1 .
    X67335 mRNA. Translation: CAA47750.1 .
    AE013599 Genomic DNA. Translation: AAF59180.1 .
    AE013599 Genomic DNA. Translation: AAS64897.1 .
    AE013599 Genomic DNA. Translation: ABV53719.1 .
    AE013599 Genomic DNA. Translation: ABV53720.1 .
    BT003323 mRNA. Translation: AAO25083.1 .
    PIRi A46140.
    B46140.
    RefSeqi NP_001097219.1. NM_001103749.2. [Q01583-4 ]
    NP_001097220.1. NM_001103750.3. [Q01583-1 ]
    NP_523654.2. NM_078930.4. [Q01583-2 ]
    NP_995775.2. NM_206053.2.
    UniGenei Dm.3102.

    3D structure databases

    ProteinModelPortali Q01583.
    SMRi Q01583. Positions 13-85, 284-358, 383-432.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1075068.

    Proteomic databases

    PaxDbi Q01583.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0112580 ; FBpp0111492 ; FBgn0085390 . [Q01583-4 ]
    GeneIDi 35738.
    KEGGi dme:Dmel_CG34361.

    Organism-specific databases

    CTDi 35738.
    FlyBasei FBgn0085390. Dgk.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00750000117569.
    InParanoidi Q01583.
    KOi K00901.
    OrthoDBi EOG75XGK8.
    PhylomeDBi Q01583.

    Miscellaneous databases

    ChiTaRSi rdgA. drosophila.
    GenomeRNAii 35738.
    NextBioi 794970.

    Gene expression databases

    Bgeei Q01583.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 2 hits.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 3 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a Drosophila diacylglycerol kinase gene that is expressed in the nervous system and muscle."
      Masai I., Hosoya T., Kojima S., Hotta Y.
      Proc. Natl. Acad. Sci. U.S.A. 89:6030-6034(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM G), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Oregon-R.
    2. "A Drosophila gene encoding a protein with similarity to diacylglycerol kinase is expressed in specific neurons."
      Harden N., Yap S.F., Chiam M.-A., Lim L.
      Biochem. J. 289:439-444(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Canton-S.
      Tissue: Embryo.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM G).
      Strain: Berkeley.
      Tissue: Testis.

    Entry informationi

    Entry nameiDGK1_DROME
    AccessioniPrimary (citable) accession number: Q01583
    Secondary accession number(s): A8DY60
    , A8DY61, Q53YF9, Q7KQR7, Q8MKP0, Q9V327
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 123 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3