Q01579 (GSTT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase theta-1
EC=2.5.1.18

Alternative name(s):
GST 5-5
GST class-theta-1
Glutathione S-transferase 5

Gene names

Name:

Gstt1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Also binds steroids, bilirubin, carcinogens and numerous organic anions. Has dichloromethane dehalogenase activity. Ref.5
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.5
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Tissue specificity	In liver, highest expression found in central vein limiting plate hepatocytes. In lung, expressed mainly in Clara cells of the bronchiolar epithelium and, at low levels, in type II alveolar cells. Ref.4
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from direct assay Ref.5. Source: UniProtKB response to drug Inferred from expression pattern PubMed 9794803. Source: RGD response to organic cyclic compound Inferred from expression pattern PubMed 9794803. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 9344408. Source: RGD
Molecular_function	glutathione peroxidase activity Inferred from direct assay PubMed 9344408. Source: RGD glutathione transferase activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 240	239	Glutathione S-transferase theta-1		PRO_0000185942
Regions
	Domain	2 – 82	81	GST N-terminal
	Domain	88 – 223	136	GST C-terminal
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	40	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01579 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 09583ACF9C85DF8F	FASTA	240	27,468
	10 20 30 40 50 60 MVLELYLDLL SQPCRAIYIF AKKNNIPFQM HTVELRKGEH LSDAFAQVNP MKKVPAMKDG 70 80 90 100 110 120 GFTLCESVAI LLYLAHKYKV PDHWYPQDLQ ARARVDEYLA WQHTTLRRSC LRTLWHKVMF 130 140 150 160 170 180 PVFLGEQIRP EMLAATLADL DVNVQVLEDQ FLQDKDFLVG PHISLADVVA ITELMHPVGG 190 200 210 220 230 240 GCPVFEGRPR LAAWYRRVEA AVGKDLFLEA HEVILKVRDC PPADPVIKQK LMPRVLTMIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An evolutionary perspective on glutathione transferases inferred from class-theta glutathione transferase cDNA sequences." Pemble S.E., Taylor J.B. Biochem. J. 287:957-963(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[3]	"Theta, a new class of glutathione transferases purified from rat and man." Meyer D.J., Coles B., Pemble S.E., Gilmore K.S., Fraser G.M., Ketterer B. Biochem. J. 274:409-414(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-47; 58-95; 120-131 AND 176-193. Tissue: Liver.
[4]	"The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human." Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T. Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY. Tissue: Liver and Lung.
[5]	"Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1." Shokeer A., Mannervik B. Biochim. Biophys. Acta 1800:466-473(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67654 mRNA. Translation: CAA47896.1. BC086426 mRNA. Translation: AAH86426.1.
IPI	IPI00231427.
PIR	S27161.
RefSeq	NP_445745.1. NM_053293.2.
UniGene	Rn.11122.
3D structure databases
ProteinModelPortal	Q01579.
SMR	Q01579. Positions 3-240.
ModBase	Search...
Proteomic databases
PaxDb	Q01579.
PRIDE	Q01579.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001669; ENSRNOP00000001669; ENSRNOG00000049771.
GeneID	25260.
KEGG	rno:25260.
UCSC	RGD:2765. rat.
Organism-specific databases
CTD	2952.
RGD	2765. Gstt1.
Phylogenomic databases
eggNOG	COG0625.
GeneTree	ENSGT00540000069741.
HOGENOM	HOG000125747.
HOVERGEN	HBG051854.
KO	K00799.
OMA	SERPHAK.
Enzyme and pathway databases
SABIO-RK	Q01579.
Gene expression databases
ArrayExpress	Q01579.
Genevestigator	Q01579.
GermOnline	ENSRNOG00000001242. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q01579.
ChEMBL	CHEMBL2423.
NextBio	605907.

Entry information

Entry name

GSTT1_RAT

Accession

Primary (citable) accession number: Q01579

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program