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Reviewed, UniProtKB/Swiss-Prot Q01578 (GNL_ZYMMO)

Last modified June 16, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Gluconolactonase
    EC=3.1.1.17
Alternative name(s):
    D-glucono-delta-lactone lactonohydrolase
Cleaved into the following 2 chains:
    1- Recommended name:
            Gluconolactonase
    2- Recommended name:
            Gluconolactonase minor isoform
Gene names
Name: gnl
Ordered Locus Names: ZMO1649
OrganismZymomonas mobilis [Complete proteome] [HAMAP]
Taxonomic identifier542 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase, and that formed in aerobic conditions by the glucose dehydrogenase present.

Catalytic activity

D-glucono-1,5-lactone + H2O = D-gluconate.

Pathway

Carbohydrate acid metabolism; D-gluconic acid biosynthesis; D-gluconic acid from D-glucono-1,5-lactone: step 1/1.

Subunit structure

Homodimer.

Subcellular location

Periplasm Probable.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Caution

It is possible that in some cases Met-40 is the initiator in this case the expressed enzyme would remain cytoplasmic.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionHydrolase
Serine esterase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiongluconolactonase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535Tat-type signal Ref.1
Chain36 – 356321Gluconolactonase
PRO_0000021336
Chain41 – 356316Gluconolactonase minor isoform
PRO_0000021337

Experimental info

Sequence conflict341Q → E in CAA47637. Ref.1
Sequence conflict1621Missing in CAA47637. Ref.1
Sequence conflict320 – 35637SGNPG…TKTFG → FW in CAA47637. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q01578-1 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 4B9D922A8AED9262

FASTA35638,453
        10         20         30         40         50         60 
MTTGRMSRRE CLSAAVMVPI AAMTATATIT GSAQAAKNNM NGSTIGKITK FSPRLDAILD 

        70         80         90        100        110        120 
VSTPIEVIAS DIQWSEGPVW VKNGNFLLFS DPPANIMRKW TPDAGVSIFL KPSGHAEPIP 

       130        140        150        160        170        180 
AGQFREPGSN GMKVGPDGKI WVADSGTRAI MKVDPVTRQR SVVVDNYKGK RFNSPNDLFF 

       190        200        210        220        230        240 
SKSGAVYFTD PPYGLTNLDE SDIKEMNYNG VFRLSPDGRL DLIEAGLSRP NGLALSPDET 

       250        260        270        280        290        300 
KLYVSNSDRA SPNIWVYSLD SNGLPTSRTL LRNFRKEYFD QGLAGLPDGM NIDKQGNLFA 

       310        320        330        340        350 
SAPGGIYIFA PDGECLGLIS GNPGQPLSNC CFGEKGQTLF ISASHNVVRV RTKTFG 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the gene encoding gluconolactonase from Zymomonas mobilis."
Kanagasundaram V., Scopes R.
Biochim. Biophys. Acta 1171:198-200(1992) [PubMed: 1482681] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-58.
Strain: ATCC 29191 / DSM 3580 / IFO 13756 / NCIB 11199 / ZM6.
[2]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

X67189 Genomic DNA. Translation: CAA47637.1.
AE008692 Genomic DNA. Translation: AAV90273.1.
PIRS28218.
RefSeqYP_163384.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3188515.
GenomeReviewsGene locus ZMO1649 in contig AE008692_GR.
KEGGzmo:ZMO1649.
NMPDRfig|264203.3.peg.492.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ01578.
OMAQ01578. FMASSHS.

Enzyme and pathway databases

BioCycMetaCyc:MON-13276.
ZMOB264203:ZMO1649-MON.
BRENDA3.1.1.17. 1658.

Family and domain databases

InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR013658. SGL.
IPR005511. SMP-30.
IPR017909. Twin_arg_translocation_Tat.
[Graphical view]
Gene3DG3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
PfamPF08450. SGL. 1 hit.
[Graphical view]
PRINTSPR01790. SMP30FAMILY.
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGNL_ZYMMO
AccessionPrimary (citable) accession number: Q01578
Secondary accession number(s): Q5NLY7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 15, 2005
Last modified: June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents