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Q01578

- GNL_ZYMMO

UniProt

Q01578 - GNL_ZYMMO

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Protein

Gluconolactonase

Gene

gnl

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase, and that formed in aerobic conditions by the glucose dehydrogenase present.

Catalytic activityi

D-glucono-1,5-lactone + H2O = D-gluconate.

Pathwayi

GO - Molecular functioni

  1. gluconolactonase activity Source: UniProtKB-EC

GO - Biological processi

  1. D-gluconate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13276.
RETL1328306-WGS:GSTH-3779-MONOMER.
UniPathwayiUPA00814; UER00785.

Names & Taxonomyi

Protein namesi
Recommended name:
Gluconolactonase (EC:3.1.1.17)
Alternative name(s):
D-glucono-delta-lactone lactonohydrolase
Cleaved into the following 2 chains:
Gene namesi
Name:gnl
Ordered Locus Names:ZMO1649
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173: Chromosome

Subcellular locationi

Periplasm Curated

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
BLAST
Chaini36 – 356321GluconolactonasePRO_0000021336Add
BLAST
Chaini41 – 356316Gluconolactonase minor isoformPRO_0000021337Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi264203.ZMO1649.

Structurei

3D structure databases

ProteinModelPortaliQ01578.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3386.
HOGENOMiHOG000075491.
KOiK01053.
OMAiVSCEHGR.
OrthoDBiEOG664CGX.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR013658. SGL.
IPR005511. SMP-30.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF08450. SGL. 1 hit.
[Graphical view]
PRINTSiPR01790. SMP30FAMILY.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01578-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTGRMSRRE CLSAAVMVPI AAMTATATIT GSAQAAKNNM NGSTIGKITK
60 70 80 90 100
FSPRLDAILD VSTPIEVIAS DIQWSEGPVW VKNGNFLLFS DPPANIMRKW
110 120 130 140 150
TPDAGVSIFL KPSGHAEPIP AGQFREPGSN GMKVGPDGKI WVADSGTRAI
160 170 180 190 200
MKVDPVTRQR SVVVDNYKGK RFNSPNDLFF SKSGAVYFTD PPYGLTNLDE
210 220 230 240 250
SDIKEMNYNG VFRLSPDGRL DLIEAGLSRP NGLALSPDET KLYVSNSDRA
260 270 280 290 300
SPNIWVYSLD SNGLPTSRTL LRNFRKEYFD QGLAGLPDGM NIDKQGNLFA
310 320 330 340 350
SAPGGIYIFA PDGECLGLIS GNPGQPLSNC CFGEKGQTLF ISASHNVVRV

RTKTFG
Length:356
Mass (Da):38,453
Last modified:February 15, 2005 - v2
Checksum:i4B9D922A8AED9262
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341Q → E in CAA47637. (PubMed:1482681)Curated
Sequence conflicti162 – 1621Missing in CAA47637. (PubMed:1482681)Curated
Sequence conflicti320 – 35637SGNPG…TKTFG → FW in CAA47637. (PubMed:1482681)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67189 Genomic DNA. Translation: CAA47637.1.
AE008692 Genomic DNA. Translation: AAV90273.1.
PIRiS28218.
RefSeqiYP_163384.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV90273; AAV90273; ZMO1649.
GeneIDi3188515.
KEGGizmo:ZMO1649.
PATRICi32568562. VBIZymMob102260_1556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67189 Genomic DNA. Translation: CAA47637.1 .
AE008692 Genomic DNA. Translation: AAV90273.1 .
PIRi S28218.
RefSeqi YP_163384.1. NC_006526.2.

3D structure databases

ProteinModelPortali Q01578.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264203.ZMO1649.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV90273 ; AAV90273 ; ZMO1649 .
GeneIDi 3188515.
KEGGi zmo:ZMO1649.
PATRICi 32568562. VBIZymMob102260_1556.

Phylogenomic databases

eggNOGi COG3386.
HOGENOMi HOG000075491.
KOi K01053.
OMAi VSCEHGR.
OrthoDBi EOG664CGX.

Enzyme and pathway databases

UniPathwayi UPA00814 ; UER00785 .
BioCyci MetaCyc:MONOMER-13276.
RETL1328306-WGS:GSTH-3779-MONOMER.

Family and domain databases

Gene3Di 2.120.10.30. 1 hit.
InterProi IPR011042. 6-blade_b-propeller_TolB-like.
IPR013658. SGL.
IPR005511. SMP-30.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF08450. SGL. 1 hit.
[Graphical view ]
PRINTSi PR01790. SMP30FAMILY.
PROSITEi PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the gene encoding gluconolactonase from Zymomonas mobilis."
    Kanagasundaram V., Scopes R.
    Biochim. Biophys. Acta 1171:198-200(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-58.
    Strain: ATCC 29191 / DSM 3580 / NBRC 13756 / NCIMB 11199 / NRRL B-4490 / ZM6.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiGNL_ZYMMO
AccessioniPrimary (citable) accession number: Q01578
Secondary accession number(s): Q5NLY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is possible that in some cases Met-40 is the initiator in this case the expressed enzyme would remain cytoplasmic.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3