Reviewed, UniProtKB/Swiss-Prot Q01578 (GNL_ZYMMO)

Names and origin

Protein names

Recommended name:
    Gluconolactonase
    EC=3.1.1.17
Alternative name(s):
    D-glucono-delta-lactone lactonohydrolase
Cleaved into the following 2 chains:
    1- Recommended name:
            Gluconolactonase
    2- Recommended name:
            Gluconolactonase minor isoform

Gene names

Name:	gnl
Ordered Locus Names:	ZMO1649

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase, and that formed in aerobic conditions by the glucose dehydrogenase present.
Catalytic activity	D-glucono-1,5-lactone + H2O = D-gluconate.
Pathway	Carbohydrate acid metabolism; D-gluconic acid biosynthesis; D-gluconic acid from D-glucono-1,5-lactone: step 1/1.
Subunit structure	Homodimer.
Subcellular location	Periplasm Probable.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Caution	It is possible that in some cases Met-40 is the initiator in this case the expressed enzyme would remain cytoplasmic.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	gluconolactonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Tat-type signal Ref.1
	Chain	36 – 356	321	Gluconolactonase		PRO_0000021336
	Chain	41 – 356	316	Gluconolactonase minor isoform		PRO_0000021337
Experimental info
	Sequence conflict	34	1	Q → E in CAA47637. Ref.1
	Sequence conflict	162	1	Missing in CAA47637. Ref.1
	Sequence conflict	320 – 356	37	SGNPG…TKTFG → FW in CAA47637. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01578-1 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: 4B9D922A8AED9262	FASTA	356	38,453
	10 20 30 40 50 60 MTTGRMSRRE CLSAAVMVPI AAMTATATIT GSAQAAKNNM NGSTIGKITK FSPRLDAILD 70 80 90 100 110 120 VSTPIEVIAS DIQWSEGPVW VKNGNFLLFS DPPANIMRKW TPDAGVSIFL KPSGHAEPIP 130 140 150 160 170 180 AGQFREPGSN GMKVGPDGKI WVADSGTRAI MKVDPVTRQR SVVVDNYKGK RFNSPNDLFF 190 200 210 220 230 240 SKSGAVYFTD PPYGLTNLDE SDIKEMNYNG VFRLSPDGRL DLIEAGLSRP NGLALSPDET 250 260 270 280 290 300 KLYVSNSDRA SPNIWVYSLD SNGLPTSRTL LRNFRKEYFD QGLAGLPDGM NIDKQGNLFA 310 320 330 340 350 SAPGGIYIFA PDGECLGLIS GNPGQPLSNC CFGEKGQTLF ISASHNVVRV RTKTFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the gene encoding gluconolactonase from Zymomonas mobilis." Kanagasundaram V., Scopes R. Biochim. Biophys. Acta 1171:198-200(1992) [PubMed: 1482681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-58. Strain: ATCC 29191 / DSM 3580 / IFO 13756 / NCIB 11199 / ZM6.
[2]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67189 Genomic DNA. Translation: CAA47637.1. AE008692 Genomic DNA. Translation: AAV90273.1.
PIR	S28218.
RefSeq	YP_163384.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3188515.
GenomeReviews	Gene locus ZMO1649 in contig AE008692_GR.
KEGG	zmo:ZMO1649.
NMPDR	fig|264203.3.peg.492.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q01578.
OMA	Q01578. FMASSHS.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13276. ZMOB264203:ZMO1649-MON.
BRENDA	3.1.1.17. 1658.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR013658. SGL. IPR005511. SMP-30. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF08450. SGL. 1 hit. [Graphical view]
PRINTS	PR01790. SMP30FAMILY.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GNL_ZYMMO

Accession

Primary (citable) accession number: Q01578
Secondary accession number(s): Q5NLY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)