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Q01578

- GNL_ZYMMO

UniProt

Q01578 - GNL_ZYMMO

Protein

Gluconolactonase

Gene

gnl

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase, and that formed in aerobic conditions by the glucose dehydrogenase present.

    Catalytic activityi

    D-glucono-1,5-lactone + H2O = D-gluconate.

    Pathwayi

    GO - Molecular functioni

    1. gluconolactonase activity Source: UniProtKB-EC

    GO - Biological processi

    1. D-gluconate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13276.
    RETL1328306-WGS:GSTH-3779-MONOMER.
    UniPathwayiUPA00814; UER00785.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gluconolactonase (EC:3.1.1.17)
    Alternative name(s):
    D-glucono-delta-lactone lactonohydrolase
    Cleaved into the following 2 chains:
    Gene namesi
    Name:gnl
    Ordered Locus Names:ZMO1649
    OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
    Taxonomic identifieri264203 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
    ProteomesiUP000001173: Chromosome

    Subcellular locationi

    Periplasm Curated

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Chaini36 – 356321GluconolactonasePRO_0000021336Add
    BLAST
    Chaini41 – 356316Gluconolactonase minor isoformPRO_0000021337Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi264203.ZMO1649.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01578.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3386.
    HOGENOMiHOG000075491.
    KOiK01053.
    OMAiVSCEHGR.
    OrthoDBiEOG664CGX.

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR013658. SGL.
    IPR005511. SMP-30.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF08450. SGL. 1 hit.
    [Graphical view]
    PRINTSiPR01790. SMP30FAMILY.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01578-1 [UniParc]FASTAAdd to Basket

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    MTTGRMSRRE CLSAAVMVPI AAMTATATIT GSAQAAKNNM NGSTIGKITK    50
    FSPRLDAILD VSTPIEVIAS DIQWSEGPVW VKNGNFLLFS DPPANIMRKW 100
    TPDAGVSIFL KPSGHAEPIP AGQFREPGSN GMKVGPDGKI WVADSGTRAI 150
    MKVDPVTRQR SVVVDNYKGK RFNSPNDLFF SKSGAVYFTD PPYGLTNLDE 200
    SDIKEMNYNG VFRLSPDGRL DLIEAGLSRP NGLALSPDET KLYVSNSDRA 250
    SPNIWVYSLD SNGLPTSRTL LRNFRKEYFD QGLAGLPDGM NIDKQGNLFA 300
    SAPGGIYIFA PDGECLGLIS GNPGQPLSNC CFGEKGQTLF ISASHNVVRV 350
    RTKTFG 356
    Length:356
    Mass (Da):38,453
    Last modified:February 15, 2005 - v2
    Checksum:i4B9D922A8AED9262
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341Q → E in CAA47637. (PubMed:1482681)Curated
    Sequence conflicti162 – 1621Missing in CAA47637. (PubMed:1482681)Curated
    Sequence conflicti320 – 35637SGNPG…TKTFG → FW in CAA47637. (PubMed:1482681)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67189 Genomic DNA. Translation: CAA47637.1.
    AE008692 Genomic DNA. Translation: AAV90273.1.
    PIRiS28218.
    RefSeqiYP_163384.1. NC_006526.2.

    Genome annotation databases

    EnsemblBacteriaiAAV90273; AAV90273; ZMO1649.
    GeneIDi3188515.
    KEGGizmo:ZMO1649.
    PATRICi32568562. VBIZymMob102260_1556.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67189 Genomic DNA. Translation: CAA47637.1 .
    AE008692 Genomic DNA. Translation: AAV90273.1 .
    PIRi S28218.
    RefSeqi YP_163384.1. NC_006526.2.

    3D structure databases

    ProteinModelPortali Q01578.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264203.ZMO1649.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV90273 ; AAV90273 ; ZMO1649 .
    GeneIDi 3188515.
    KEGGi zmo:ZMO1649.
    PATRICi 32568562. VBIZymMob102260_1556.

    Phylogenomic databases

    eggNOGi COG3386.
    HOGENOMi HOG000075491.
    KOi K01053.
    OMAi VSCEHGR.
    OrthoDBi EOG664CGX.

    Enzyme and pathway databases

    UniPathwayi UPA00814 ; UER00785 .
    BioCyci MetaCyc:MONOMER-13276.
    RETL1328306-WGS:GSTH-3779-MONOMER.

    Family and domain databases

    Gene3Di 2.120.10.30. 1 hit.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR013658. SGL.
    IPR005511. SMP-30.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF08450. SGL. 1 hit.
    [Graphical view ]
    PRINTSi PR01790. SMP30FAMILY.
    PROSITEi PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the gene encoding gluconolactonase from Zymomonas mobilis."
      Kanagasundaram V., Scopes R.
      Biochim. Biophys. Acta 1171:198-200(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-58.
      Strain: ATCC 29191 / DSM 3580 / NBRC 13756 / NCIMB 11199 / NRRL B-4490 / ZM6.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31821 / ZM4 / CP4.

    Entry informationi

    Entry nameiGNL_ZYMMO
    AccessioniPrimary (citable) accession number: Q01578
    Secondary accession number(s): Q5NLY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    It is possible that in some cases Met-40 is the initiator in this case the expressed enzyme would remain cytoplasmic.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3