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Q01578 (GNL_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gluconolactonase

EC=3.1.1.17
Alternative name(s):
D-glucono-delta-lactone lactonohydrolase

Cleaved into the following 2 chains:

  1. Gluconolactonase
  2. Gluconolactonase minor isoform
Gene names
Name:gnl
Ordered Locus Names:ZMO1649
OrganismZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]
Taxonomic identifier264203 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase, and that formed in aerobic conditions by the glucose dehydrogenase present.

Catalytic activity

D-glucono-1,5-lactone + H2O = D-gluconate.

Pathway

Carbohydrate acid metabolism; D-gluconate biosynthesis; D-gluconate from D-glucono-1,5-lactone: step 1/1.

Subunit structure

Homodimer.

Subcellular location

Periplasm Probable.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Caution

It is possible that in some cases Met-40 is the initiator in this case the expressed enzyme would remain cytoplasmic.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionHydrolase
Serine esterase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processD-gluconate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiongluconolactonase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535Tat-type signal Ref.1
Chain36 – 356321Gluconolactonase
PRO_0000021336
Chain41 – 356316Gluconolactonase minor isoform
PRO_0000021337

Experimental info

Sequence conflict341Q → E in CAA47637. Ref.1
Sequence conflict1621Missing in CAA47637. Ref.1
Sequence conflict320 – 35637SGNPG…TKTFG → FW in CAA47637. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q01578 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 4B9D922A8AED9262

FASTA35638,453
        10         20         30         40         50         60 
MTTGRMSRRE CLSAAVMVPI AAMTATATIT GSAQAAKNNM NGSTIGKITK FSPRLDAILD 

        70         80         90        100        110        120 
VSTPIEVIAS DIQWSEGPVW VKNGNFLLFS DPPANIMRKW TPDAGVSIFL KPSGHAEPIP 

       130        140        150        160        170        180 
AGQFREPGSN GMKVGPDGKI WVADSGTRAI MKVDPVTRQR SVVVDNYKGK RFNSPNDLFF 

       190        200        210        220        230        240 
SKSGAVYFTD PPYGLTNLDE SDIKEMNYNG VFRLSPDGRL DLIEAGLSRP NGLALSPDET 

       250        260        270        280        290        300 
KLYVSNSDRA SPNIWVYSLD SNGLPTSRTL LRNFRKEYFD QGLAGLPDGM NIDKQGNLFA 

       310        320        330        340        350 
SAPGGIYIFA PDGECLGLIS GNPGQPLSNC CFGEKGQTLF ISASHNVVRV RTKTFG 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the gene encoding gluconolactonase from Zymomonas mobilis."
Kanagasundaram V., Scopes R.
Biochim. Biophys. Acta 1171:198-200(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-58.
Strain: ATCC 29191 / DSM 3580 / NBRC 13756 / NCIMB 11199 / NRRL B-4490 / ZM6.
[2]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67189 Genomic DNA. Translation: CAA47637.1.
AE008692 Genomic DNA. Translation: AAV90273.1.
PIRS28218.
RefSeqYP_163384.1. NC_006526.2.

3D structure databases

ProteinModelPortalQ01578.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264203.ZMO1649.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV90273; AAV90273; ZMO1649.
GeneID3188515.
KEGGzmo:ZMO1649.
PATRIC32568562. VBIZymMob102260_1556.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3386.
HOGENOMHOG000075491.
KOK01053.
OMAPAIWAEG.
OrthoDBEOG664CGX.
ProtClustDBCLSK2524949.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13276.
UniPathwayUPA00814; UER00785.

Family and domain databases

Gene3D2.120.10.30. 1 hit.
InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR013658. SGL.
IPR005511. SMP-30.
IPR006311. TAT_signal.
[Graphical view]
PfamPF08450. SGL. 1 hit.
[Graphical view]
PRINTSPR01790. SMP30FAMILY.
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGNL_ZYMMO
AccessionPrimary (citable) accession number: Q01578
Secondary accession number(s): Q5NLY7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 15, 2005
Last modified: January 22, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways