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Protein

Nucleolar protein 3

Gene

NPL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A).

Miscellaneous

Present with 78700 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • eukaryotic initiation factor 4G binding Source: SGD
  • identical protein binding Source: IntAct
  • mRNA binding Source: SGD
  • poly(A) binding Source: SGD
  • RNA polymerase II core binding Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
  • negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD
  • negative regulation of translation Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • rRNA processing Source: UniProtKB-KW
  • translational termination Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29970-MONOMER.
ReactomeiR-SCE-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 3
Alternative name(s):
Mitochondrial targeting suppressor 1 protein
Nuclear polyadenylated RNA-binding protein 1
Gene namesi
Name:NPL3
Synonyms:MTR13, MTS1, NAB1, NOP3
Ordered Locus Names:YDR432W
ORF Names:D9461.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR432W.
SGDiS000002840. NPL3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816761 – 414Nucleolar protein 3Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01560.
PRIDEiQ01560.

PTM databases

iPTMnetiQ01560.

Interactioni

Subunit structurei

Interacts with RRP6.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • eukaryotic initiation factor 4G binding Source: SGD
  • identical protein binding Source: IntAct
  • RNA polymerase II core binding Source: SGD

Protein-protein interaction databases

BioGridi32489. 1138 interactors.
DIPiDIP-6464N.
IntActiQ01560. 39 interactors.
MINTiMINT-600838.
STRINGi4932.YDR432W.

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi125 – 129Combined sources5
Helixi138 – 145Combined sources8
Turni146 – 148Combined sources3
Beta strandi153 – 157Combined sources5
Beta strandi160 – 164Combined sources5
Helixi168 – 178Combined sources11
Beta strandi189 – 192Combined sources4
Beta strandi200 – 205Combined sources6
Helixi213 – 223Combined sources11
Beta strandi228 – 231Combined sources4
Beta strandi236 – 238Combined sources3
Beta strandi241 – 247Combined sources7
Helixi248 – 257Combined sources10
Beta strandi261 – 263Combined sources3
Beta strandi266 – 273Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JD5X-ray2.50C408-414[»]
2JVONMR-A116-201[»]
2JVRNMR-A193-282[»]
2OSQNMR-A121-194[»]
2OSRNMR-A194-280[»]
ProteinModelPortaliQ01560.
SMRiQ01560.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01560.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 195RRM 1PROSITE-ProRule annotationAdd BLAST71
Domaini200 – 275RRM 2PROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi280 – 398Arg/Gly-richAdd BLAST119

Sequence similaritiesi

Belongs to the RRM GAR family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00700000104103.
InParanoidiQ01560.
KOiK14651.
OMAiYYDSTGY.
OrthoDBiEOG092C5ATD.

Family and domain databases

CDDicd12340. RBD_RRM1_NPL3. 1 hit.
InterProiView protein in InterPro
IPR034166. Npl3_RRM1.
IPR035979. RBD_domain_sf.
IPR000504. RRM_dom.
PfamiView protein in Pfam
PF00076. RRM_1. 2 hits.
SMARTiView protein in SMART
SM00360. RRM. 2 hits.
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiView protein in PROSITE
PS50102. RRM. 2 hits.

Sequencei

Sequence statusi: Complete.

Q01560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAQETHVE QLPESVVDAP VEEQHQEPPQ APDAPQEPQV PQESAPQESA
60 70 80 90 100
PQEPPAPQEQ NDVPPPSNAP IYEGEESHSV QDYQEAHQHH QPPEPQPYYP
110 120 130 140 150
PPPPGEHMHG RPPMHHRQEG ELSNTRLFVR PFPLDVQESE LNEIFGPFGP
160 170 180 190 200
MKEVKILNGF AFVEFEEAES AAKAIEEVHG KSFANQPLEV VYSKLPAKRY
210 220 230 240 250
RITMKNLPEG CSWQDLKDLA RENSLETTFS SVNTRDFDGT GALEFPSEEI
260 270 280 290 300
LVEALERLNN IEFRGSVITV ERDDNPPPIR RSNRGGFRGR GGFRGGFRGG
310 320 330 340 350
FRGGFSRGGF GGPRGGFGGP RGGYGGYSRG GYGGYSRGGY GGSRGGYDSP
360 370 380 390 400
RGGYDSPRGG YSRGGYGGPR NDYGPPRGSY GGSRGGYDGP RGDYGPPRDA
410
YRTRDAPRER SPTR
Length:414
Mass (Da):45,407
Last modified:July 1, 1993 - v1
Checksum:i024439E4B6578787
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66019 Genomic DNA. Translation: CAA46817.1.
M86731 Genomic DNA. Translation: AAA34818.1.
X70951 Genomic DNA. Translation: CAA50291.1.
U33007 Genomic DNA. Translation: AAB64865.1.
BK006938 Genomic DNA. Translation: DAA12270.1.
PIRiJN0866.
RefSeqiNP_010720.3. NM_001180740.3.

Genome annotation databases

EnsemblFungiiYDR432W; YDR432W; YDR432W.
GeneIDi852042.
KEGGisce:YDR432W.

Similar proteinsi

Entry informationi

Entry nameiNOP3_YEAST
AccessioniPrimary (citable) accession number: Q01560
Secondary accession number(s): D6VT60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 22, 2017
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names