Q01559 (HMDH_NICSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase
Short name=HMG-CoA reductase
EC=1.1.1.34

Gene names

Name:

HMGR

Organism

Nicotiana sylvestris (Wood tobacco) (South American tobacco)

Taxonomic identifier

4096 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate, the specific precursor of all isoprenoid compounds present in plants. Possible role in plant defense mechanisms as well as in the cell cycle.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Found in protoplasts and leaves submitted to stress. Low levels found in apexes, anthers and roots.
Developmental stage	Expressed at highest levels during the G0-G1 transition in the cell cycle of mesophyll protoplasts.
Induction	In leaves, by wounding, elicitation, bacterial or fungal infection.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 604	604	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114445
Regions
	Transmembrane	40 – 62	23	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Region	111 – 189	79	Linker By similarity
	Region	190 – 604	415	Catalytic By similarity
Sites
	Active site	283	1	Charge relay system By similarity
	Active site	415	1	Charge relay system By similarity
	Active site	491	1	Charge relay system By similarity
	Active site	589	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	460	1	N-linked (GlcNAc...) Potential
	Glycosylation	593	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01559 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7046D17CAC0D0BA4	FASTA	604	65,085
	10 20 30 40 50 60 MDVRRRSEKP AYPTKEFAAG EKPLKPHKQQ QEQDNSLLIA SDALPLPLYL TNGLFFTMFF 70 80 90 100 110 120 SVMYYLLSRW REKIRNSTPL HVVTFSELVA IASLIASVIY LLGFFGIGFV QSFVSRDNND 130 140 150 160 170 180 ECWDEEDEND EQFLLEEDSR RGPATTLGCT AVPPPPALQI VPMVPPQPSK VAAMSEKPAP 190 200 210 220 230 240 LVTPAASEED EEIIKSVVQG KMPSYSLESK LGDCKRAASI RKEALQRITG KSLEGLPLEG 250 260 270 280 290 300 FDYESILGQC CEMPIGYVQI PVGIAGPLLL DGREYSVPMA TTEGCLVAST NRGCKAIYAS 310 320 330 340 350 360 GGATSVLLRD GMTRAPCVRF GTAKRAAELK FFVEDPVKFE TLAAVFNQSS RFARLQRIQC 370 380 390 400 410 420 AIAGKNLYMR FVCSTGDAMG MNMVSKGVQN VLDYLQNEYP DMDVIGISGN FCSDKKPAAV 430 440 450 460 470 480 NWIEGRGKSV VCEAIITEEV VKKVLKTEVA ALVELNMLKN LTGSAMAGAL GGFNAHASNI 490 500 510 520 530 540 VSAVYIATGQ DPAQNIESSH CITMMEAVND GKDLHVSVTM PSIEVGTVGG GTQLASQSAC 550 560 570 580 590 600 LNLLGVKGAN REVPGSNARL LATIVAGSVL AGELSLMSAI SAGQLVKSHM KYNRSTKDVT KASS

References

[1]	"Isolation and characterization of a cDNA encoding a 3-hydroxy-3-methylglutaryl coenzyme A reductase from Nicotiana sylvestris." Genschik P., Criqui M.-C., Parmentier Y., Marbach J., Durr A., Fleck J., Jamet E. Plant Mol. Biol. 20:337-341(1992) [PubMed: 1391779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Protoplast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63649 mRNA. Translation: CAA45181.1.
PIR	S24760.
3D structure databases
ProteinModelPortal	Q01559.
SMR	Q01559. Positions 186-586.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_NICSY

Accession

Primary (citable) accession number: Q01559

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 28, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program