Q01559 (HMDH_NICSY) Reviewed, UniProtKB/Swiss-Prot
Last modified
June 28, 2011.
Version 82.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase Short name=HMG-CoA reductase EC=1.1.1.34 | ||
| Gene names |
| ||
| Organism | Nicotiana sylvestris (Wood tobacco) (South American tobacco) | ||
| Taxonomic identifier | 4096 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana |
Protein attributes
| Sequence length | 604 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Catalyzes the synthesis of mevalonate, the specific precursor of all isoprenoid compounds present in plants. Possible role in plant defense mechanisms as well as in the cell cycle. |
| Catalytic activity | (R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Tissue specificity | Found in protoplasts and leaves submitted to stress. Low levels found in apexes, anthers and roots. |
| Developmental stage | Expressed at highest levels during the G0-G1 transition in the cell cycle of mesophyll protoplasts. |
| Induction | In leaves, by wounding, elicitation, bacterial or fungal infection. |
| Sequence similarities | Belongs to the HMG-CoA reductase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Isoprene biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| PTM | Glycoprotein |
| Gene Ontology (GO) | |
| Biological process | coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 604 | 604 | 3-hydroxy-3-methylglutaryl-coenzyme A reductase | PRO_0000114445 | |||||
Regions | |||||||||
| Transmembrane | 40 – 62 | 23 | Helical; Potential | ||||||
| Transmembrane | 90 – 110 | 21 | Helical; Potential | ||||||
| Region | 111 – 189 | 79 | Linker By similarity | ||||||
| Region | 190 – 604 | 415 | Catalytic By similarity | ||||||
Sites | |||||||||
| Active site | 283 | 1 | Charge relay system By similarity | ||||||
| Active site | 415 | 1 | Charge relay system By similarity | ||||||
| Active site | 491 | 1 | Charge relay system By similarity | ||||||
| Active site | 589 | 1 | Proton donor By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 347 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 460 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 593 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| [1] | "Isolation and characterization of a cDNA encoding a 3-hydroxy-3-methylglutaryl coenzyme A reductase from Nicotiana sylvestris." Genschik P., Criqui M.-C., Parmentier Y., Marbach J., Durr A., Fleck J., Jamet E. Plant Mol. Biol. 20:337-341(1992) [PubMed: 1391779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Protoplast. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X63649 mRNA. Translation: CAA45181.1. |
| PIR | S24760. |
3D structure databases | |
| ProteinModelPortal | Q01559. |
| SMR | Q01559. Positions 186-586. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view] |
| Gene3D | G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit. |
| PANTHER | PTHR10572. HMG-CoA_red. 1 hit. |
| Pfam | PF00368. HMG-CoA_red. 1 hit. [Graphical view] |
| PRINTS | PR00071. HMGCOARDTASE. |
| SUPFAM | SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit. |
| TIGRFAMs | TIGR00533. HMG_CoA_R_NADP. 1 hit. |
| PROSITE | PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HMDH_NICSY | ||||||||
| Accession | Primary (citable) accession number: Q01559 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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