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Reviewed, UniProtKB/Swiss-Prot Q01551 (TBUD_BURPI)

Last modified June 16, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phenol 2-monooxygenase
    EC=1.14.13.7
Alternative name(s):
    Phenol hydroxylase
Gene names
Name: tbuD
OrganismBurkholderia pickettii (Ralstonia pickettii) (Pseudomonas pickettii)
Taxonomic identifier329 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

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Protein attributes

Sequence length671 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Hydroxylates phenol to catechol. Also acts on cresols.

Catalytic activity

Phenol + NADPH + O2 = catechol + NADP+ + H2O.

Cofactor

FAD.

Pathway

Aromatic compound metabolism; phenol degradation.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the pheA/tfdB FAD monooxygenase family.

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Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionphenol 2-monooxygenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 671670Phenol 2-monooxygenase
PRO_0000214047

Regions

Nucleotide binding10 – 4334FAD Potential
Nucleotide binding295 – 30511FAD Potential

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Sequences

Sequence LengthMass (Da)Tools
Q01551-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: A7ADFB9286640195

FASTA67172,999
        10         20         30         40         50         60 
MTKYNEAYCD VLIVGAGPAG VMAAAHLLSY GTTARPHRVR IFDATKEVNG SDESTESLST 

        70         80         90        100        110        120 
DVIADALNSG ASGPEKDAAS TTEDLPMLVT TLQVSDVLHD TGDDTKIAYR ETATEQQVLL 

       130        140        150        160        170        180 
LADTTANTSS TMNPRSMCEA GCRFHQIYQG HCFPEYELDS ERLRSVDGRA QVLEDEHETG 

       190        200        210        220        230        240 
QLRLERLGRP EELLELDEEN SMSVVTNLKA APYKFLMKDV DENFPGELST SGGKTTSISA 

       250        260        270        280        290        300 
DESAIDAALH AVWDADDLGA AWHLDEASGL RAVDWNAAQW FKSGQPWTPD AAKSLQEGRV 

       310        320        330        340        350        360 
FLAGDARHRH PPLTGIGKNT SIADCYNLTW KLLGVLLGVA RADPARTYVA ERVYIRMRAA 

       370        380        390        400        410        420 
TDIAVDAEME SLAAKWITVQ LTLSRSWISS AKEAERWDAV LRDSAMSASK PMWTTSDMRA 

       430        440        450        460        470        480 
SFDAGLMGHG HAHDHVTPTI KEFASSSISR SISELASTSW WESRGWGNGG PFESLMEDAR 

       490        500        510        520        530        540 
WTGAVESNCR YAAYDRDAPV LHEHVAWVTR FTSRARTAVL EAAVGQAHVV DCWDVGLVEP 

       550        560        570        580        590        600 
ALDDLDSAGA GLHVAHHADQ WPAQLDEAVW PRESLSDWRI VTDTSATGEG YQTSPREAPG 

       610        620        630        640        650        660 
DYADLNADNA KAHFNGQFAG HKAYGDAAAA DGGGCHGRIL VGPAVRGRHL HREIPLGEEC 

       670 
QRAAQPLFKE V

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References

[1]"Complete nucleotide sequence of tbuD, the gene encoding phenol/cresol hydroxylase from Pseudomonas pickettii PKO1, and functional analysis of the encoded enzyme."
Kukor J.J., Olsen R.H.
J. Bacteriol. 174:6518-6526(1992) [PubMed: 1400204] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-22.
Strain: PKO1.

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Cross-references

Sequence databases

M98806 Genomic DNA. Translation: AAA25992.1.
PIRA45730.

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BRENDA1.14.13.7. 276669.

Family and domain databases

InterProIPR002938. mOase_FAD_bd.
IPR003042. Rng_hydrolase-like.
[Graphical view]
PfamPF01494. FAD_binding_3. 2 hits.
[Graphical view]
PRINTSPR00420. RNGMNOXGNASE.
ProtoNetSearch...

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Entry information

Entry nameTBUD_BURPI
AccessionPrimary (citable) accession number: Q01551
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents