Reviewed, UniProtKB/Swiss-Prot Q01551 (TBUD_BURPI)

Names and origin

Protein names

Recommended name:
    Phenol 2-monooxygenase
    EC=1.14.13.7
Alternative name(s):
    Phenol hydroxylase

Gene names

Name:

tbuD

Organism

Burkholderia pickettii (Ralstonia pickettii) (Pseudomonas pickettii)

Taxonomic identifier

329 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydroxylates phenol to catechol. Also acts on cresols.
Catalytic activity	Phenol + NADPH + O(2) = catechol + NADP(+) + H(2)O.
Cofactor	FAD.
Pathway	Aromatic compound metabolism; phenol degradation.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the pheA/tfdB FAD monooxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phenol 2-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 671	670	Phenol 2-monooxygenase		PRO_0000214047
Regions
	Nucleotide binding	10 – 43	34	FAD Potential
	Nucleotide binding	295 – 305	11	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01551-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A7ADFB9286640195	FASTA	671	72,999
	10 20 30 40 50 60 MTKYNEAYCD VLIVGAGPAG VMAAAHLLSY GTTARPHRVR IFDATKEVNG SDESTESLST 70 80 90 100 110 120 DVIADALNSG ASGPEKDAAS TTEDLPMLVT TLQVSDVLHD TGDDTKIAYR ETATEQQVLL 130 140 150 160 170 180 LADTTANTSS TMNPRSMCEA GCRFHQIYQG HCFPEYELDS ERLRSVDGRA QVLEDEHETG 190 200 210 220 230 240 QLRLERLGRP EELLELDEEN SMSVVTNLKA APYKFLMKDV DENFPGELST SGGKTTSISA 250 260 270 280 290 300 DESAIDAALH AVWDADDLGA AWHLDEASGL RAVDWNAAQW FKSGQPWTPD AAKSLQEGRV 310 320 330 340 350 360 FLAGDARHRH PPLTGIGKNT SIADCYNLTW KLLGVLLGVA RADPARTYVA ERVYIRMRAA 370 380 390 400 410 420 TDIAVDAEME SLAAKWITVQ LTLSRSWISS AKEAERWDAV LRDSAMSASK PMWTTSDMRA 430 440 450 460 470 480 SFDAGLMGHG HAHDHVTPTI KEFASSSISR SISELASTSW WESRGWGNGG PFESLMEDAR 490 500 510 520 530 540 WTGAVESNCR YAAYDRDAPV LHEHVAWVTR FTSRARTAVL EAAVGQAHVV DCWDVGLVEP 550 560 570 580 590 600 ALDDLDSAGA GLHVAHHADQ WPAQLDEAVW PRESLSDWRI VTDTSATGEG YQTSPREAPG 610 620 630 640 650 660 DYADLNADNA KAHFNGQFAG HKAYGDAAAA DGGGCHGRIL VGPAVRGRHL HREIPLGEEC 670 QRAAQPLFKE V

References

[1]

"Complete nucleotide sequence of tbuD, the gene encoding phenol/cresol hydroxylase from Pseudomonas pickettii PKO1, and functional analysis of the encoded enzyme." Kukor J.J., Olsen R.H. J. Bacteriol. 174:6518-6526(1992) [PubMed: 1400204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-22. Strain: PKO1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M98806 Genomic DNA. Translation: AAA25992.1.
PIR	A45730.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase. [Graphical view]
Pfam	PF01494. FAD_binding_3. 2 hits. [Graphical view]
PRINTS	PR00368. FADPNR. PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

TBUD_BURPI

Accession

Primary (citable) accession number: Q01551

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)