Reviewed, UniProtKB/Swiss-Prot Q01543 (FLI1_HUMAN)

Names and origin

Protein names

Recommended name:
    Friend leukemia integration 1 transcription factor
Alternative name(s):
    Fli-1 proto-oncogene
    ERGB transcription factor

Gene names

Name:

FLI1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Subunit structure	Can form homodimers or heterodimers with ETV6/TEL1.
Subcellular location	Nucleus.
Involvement in disease	A chromosomal aberration involving FLI1 is a cause of Ewing sarcoma [MIM:133450]. Translocation t(11;22)(q24;q12) with EWS.
Miscellaneous	Located on a fragment of chromosome 11 flanked on the centromeric side by the acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation breakpoint and on the telomeric side by the Ewing- and neuroepithelioma-associated t(11;22) (q24;q12) breakpoint.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement
Disease	Proto-oncogene
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	hemostasis Traceable author statement. Source: ProtInc organ morphogenesis Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Grip1	Q925T6	1	EBI-2271018,EBI-537752	From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q01543-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q01543-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: MDGTIKEALS...KREYDHMNGS → MEGGLAGERA

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 452

452

Friend leukemia integration 1 transcription factor

PRO_0000204124

Regions

Domain

112 – 198

87

PNT

DNA binding

281 – 361

81

ETS

Amino acid modifications

Modified residue

76

1

Phosphoserine Ref.11

Modified residue

86

1

Phosphoserine Ref.11

Modified residue

89

1

Phosphoserine Ref.11

Natural variations

Alternative sequence

1 – 76

76

MDGTI…HMNGS → MEGGLAGERA in isoform 2.

VSP_001478

Experimental info

Sequence conflict

69

1

E → V in AAA58479. Ref.5

Sequence conflict

77

1

Missing in AAA58479. Ref.5

Sequence conflict

130

1

P → A in AAA58479. Ref.5

Sequence conflict

130

1

P → A in AAA58480. Ref.5

Sequence conflict

133

1

W → V in AAA58479. Ref.5

Sequence conflict

133

1

W → V in AAA58480. Ref.5

Sequence conflict

323

1

E → Q Ref.4

Sequence conflict

323

1

E → Q Ref.5

Sequence conflict

426

1

Missing in AAA58479. Ref.5

Sequence conflict

426

1

Missing in AAA58480. Ref.5

Secondary structure

1

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452

Helix Strand Turn

Details...

Beta strand

120 – 122

3

Helix

130 – 132

3

Helix

137 – 148

12

Helix

156 – 159

4

Helix

164 – 169

6

Helix

172 – 176

5

Helix

181 – 195

15

Helix

284 – 292

9

Beta strand

298 – 301

4

Beta strand

305 – 308

4

Helix

314 – 324

11

Helix

333 – 344

12

Helix

352 – 354

3

Beta strand

355 – 357

3

Beta strand

366 – 368

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 9C2AAEEAF683F3FA	FASTA	452	50,982
	10 20 30 40 50 60 MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP LPPQQEWINQ 70 80 90 100 110 120 PVRVNVKREY DHMNGSRESP VDCSVSKCSK LVGGGESNPM NYNSYMDEKN GPPPPNMTTN 130 140 150 160 170 180 ERRVIVPADP TLWTQEHVRQ WLEWAIKEYS LMEIDTSFFQ NMDGKELCKM NKEDFLRATT 190 200 210 220 230 240 LYNTEVLLSH LSYLRESSLL AYNTTSHTDQ SSRLSVKEDP SYDSVRRGAW GNNMNSGLNK 250 260 270 280 290 300 SPPLGGAQTI SKNTEQRPQP DPYQILGPTS SRLANPGSGQ IQLWQFLLEL LSDSANASCI 310 320 330 340 350 360 TWEGTNGEFK MTDPDEVARR WGERKSKPNM NYDKLSRALR YYYDKNIMTK VHGKRYAYKF 370 380 390 400 410 420 DFHGIAQALQ PHPTESSMYK YPSDISYMPS YHAHQQKVNF VPPHPSSMPV TSSSFFGAAS 430 440 450 QYWTSPTGGI YPNPNVPRHP NTHVPSHLGS YY
	Isoform 2. Checksum: BEA132F4038A230C	FASTA	386	43,649
	10 20 30 40 50 60 MEGGLAGERA RESPVDCSVS KCSKLVGGGE SNPMNYNSYM DEKNGPPPPN MTTNERRVIV 70 80 90 100 110 120 PADPTLWTQE HVRQWLEWAI KEYSLMEIDT SFFQNMDGKE LCKMNKEDFL RATTLYNTEV 130 140 150 160 170 180 LLSHLSYLRE SSLLAYNTTS HTDQSSRLSV KEDPSYDSVR RGAWGNNMNS GLNKSPPLGG 190 200 210 220 230 240 AQTISKNTEQ RPQPDPYQIL GPTSSRLANP GSGQIQLWQF LLELLSDSAN ASCITWEGTN 250 260 270 280 290 300 GEFKMTDPDE VARRWGERKS KPNMNYDKLS RALRYYYDKN IMTKVHGKRY AYKFDFHGIA 310 320 330 340 350 360 QALQPHPTES SMYKYPSDIS YMPSYHAHQQ KVNFVPPHPS SMPVTSSSFF GAASQYWTSP 370 380 TGGIYPNPNV PRHPNTHVPS HLGSYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours." Delattre O., Zucman J., Plougastel B., Desmaze C., Melot T., Peter M., Kovar H., Joubert I., de Jong P., Rouleau G., Aurias A., Thomas G. Nature 359:162-165(1992) [PubMed: 1522903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Bone marrow.
[2]	"The ERGB/Fli-1 gene: isolation and characterization of a new member of the family of human ETS transcription factors." Watson D.K., Smyth F.E., Thompson D.M., Cheng J.Q., Testa J.R., Papas T.S., Seth A. Cell Growth Differ. 3:705-713(1992) [PubMed: 1445800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	Watson D. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"Structure and expression of human Fli-1 gene." Prasad D.D., Rao V.N., Reddy E.S. Cancer Res. 52:5833-5837(1992) [PubMed: 1394211] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"Human FLI-1 localizes to chromosome 11q24 and has an aberrant transcript in neuroepithelioma." Hromas R., May W., Denny C., Raskind W., Moore J., Maki R.A., Beck E., Klemsz M.J. Biochim. Biophys. Acta 1172:155-158(1993) [PubMed: 8439553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Blood.
[6]	"The human homolog of the mouse common viral integration region, FLI1, maps to 11q23-q24." Baud V., Lipinski M., Rassart E., Poliquin L., Bergeron D. Genomics 11:223-224(1991) [PubMed: 1765382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHROMOSOMAL TRANSLOCATION.
[7]	Ubhi B.T.S., Rainey D.R., Meredith D.M. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph.
[9]	"Chromosome translocation based on illegitimate recombination in human tumors." Zucman-Rossi J., Legoix P., Victor J.M., Lopez B., Thomas G. Proc. Natl. Acad. Sci. U.S.A. 95:11786-11791(1998) [PubMed: 9751743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-452.
[10]	"Molecular analysis of a t(11;22) translocation junction in a case of Ewing's sarcoma." Bhagirath T., Abe S., Nojima T., Yoshida M.C. Genes Chromosomes Cancer 13:126-132(1995) [PubMed: 7542907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 278-301. Tissue: Placenta.
[11]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-86 AND SER-89, MASS SPECTROMETRY.
[12]	"Solution structure of the ets domain of Fli-1 when bound to DNA." Liang H., Mao X., Olejniczak E.T., Nettesheim D.G., Yu L., Meadows R.P., Thompson C.B., Fesik S.W. Nat. Struct. Biol. 1:871-875(1994) [PubMed: 7773776] [Abstract] Cited for: STRUCTURE BY NMR OF 276-373.
[13]	"Solution structure of the SAM_pnt-domain of the human Friend leukemia integration 1 transcription factor." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 114-198.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X67001 mRNA. Translation: CAA47399.1.
M98833 mRNA. Translation: AAA35812.2.
S45205 mRNA. Translation: AAB23637.1.
M93255 mRNA. Translation: AAA58479.1.
M93255 mRNA. Translation: AAA58480.1.
AY029368 mRNA. Translation: AAK50443.1.
BC001670 mRNA. Translation: AAH01670.1.
BC010115 mRNA. Translation: AAH10115.1.
Y17293 Genomic DNA. Translation: CAA76731.1.
D38408 Genomic DNA. Translation: BAA07463.1. Different termination.

IPI

IPI00217470.
IPI00329755.

PIR

I37565.
S29844.

RefSeq

NP_002008.2.

UniGene

Hs.504281

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FLI	NMR	-	A	276-373	[»]
1X66	NMR	-	A	114-198	[»]
2YTU	NMR	-	A	100-220	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q01543. 1 interaction.

PTM databases

PhosphoSite

Q01543.

Genome annotation databases

Ensembl

ENSG00000151702. Homo sapiens. [Contig view]

GeneID

2313.

KEGG

hsa:2313.

Organism-specific databases

GeneCards

GC11P128069.

H-InvDB

HIX0010262.

HGNC

HGNC:3749. FLI1.

MIM

133450. gene+phenotype.
193067. gene.

Orphanet

319. Ewing sarcoma.
851. Paris-Trousseau thrombocytopenia.

PharmGKB

PA28170.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q01543.

HOVERGEN

Q01543.

Gene expression databases

ArrayExpress

Q01543.

Bgee

Q01543.

CleanEx

HS_FLI1.

GermOnline

ENSG00000151702. Homo sapiens.

Family and domain databases

InterPro

IPR000418. Ets.
IPR003118. SAM_PNT.
IPR013761. SAM_type.
IPR011991. Wing_hlx_DNA_bd.
[Graphical view]

Gene3D

G3DSA:1.10.150.50. SAM_type. 1 hit.
G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]

PRINTS

PR00454. ETSDOMAIN.

SMART

SM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]

PROSITE

PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

9393.

PMAP-CutDB

Q01543.

SOURCE

Search...

Entry information

Entry name

FLI1_HUMAN

Accession

Primary (citable) accession number: Q01543
Secondary accession number(s): Q14319, Q92480, Q9UE07

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)