Reviewed, UniProtKB/Swiss-Prot Q01524 (DEF6_HUMAN)

Names and origin

Protein names

Recommended name:
    Defensin-6
Alternative name(s):
    Defensin, alpha 6

Gene names

Name:	DEFA6
Synonyms:	DEF6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. May protect cells against infection with HIV-1.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Tissue specificity	Paneth cells of the small intestine.
Sequence similarities	Belongs to the alpha-defensin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Defensin Fungicide
Technical term	3D-structure
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Propeptide

20 – ?65

46

Potential

PRO_0000006790

Peptide

?66 – 100

35

Defensin-6

PRO_0000006791

Amino acid modifications

Disulfide bond

72 ↔ 99

Disulfide bond

74 ↔ 88

Disulfide bond

78 ↔ 98

Secondary structure

1

.

.

.

.

.

100

Helix Strand Turn

Details...

Beta strand

71 – 77

7

Beta strand

82 – 90

9

Sequences

	Sequence		Length	Mass (Da)
	Q01524-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: EDF77E033DDCE2D5	FASTA	100	10,975
	10 20 30 40 50 60 MRTLTILTAV LLVALQAKAE PLQAEDDPLQ AKAYEADAQE QRGANDQDFA VSFAEDASSS 70 80 90 100 LRALGSTRAF TCHCRRSCYS TEYSYGTCTV MGINHRFCCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Defensin-6 mRNA in human Paneth cells: implications for antimicrobial peptides in host defense of the human bowel." Jones D.E., Bevins C.L. FEBS Lett. 315:187-192(1993) [PubMed: 8417977] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine.
[2]	"Human enteric defensins. Gene structure and developmental expression." Mallow E.B., Harris A., Salzman N., Russell J.P., Deberardinis R.J., Ruchelli E., Bevins C.L. J. Biol. Chem. 271:4038-4045(1996) [PubMed: 8626737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Antibacterial activity and specificity of the six human alpha-defensins." Ericksen B., Wu Z., Lu W., Lehrer R.I. Antimicrob. Agents Chemother. 49:269-275(2005) [PubMed: 15616305] [Abstract] Cited for: FUNCTION.
[5]	"Crystal structures of human alpha-defensins HNP4, HD5, and HD6." Szyk A., Wu Z., Tucker K., Yang D., Lu W., Lubkowski J. Protein Sci. 15:2749-2760(2006) [PubMed: 17088326] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 69-100, FUNCTION, SUBUNIT, DISUFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M98331 mRNA. Translation: AAB59357.1.
U33317 Genomic DNA. Translation: AAC50382.1. Sequence problems.
BC069667 mRNA. Translation: AAH69667.1.
BC069710 mRNA. Translation: AAH69710.1.
BC069728 mRNA. Translation: AAH69728.1.
BC069769 mRNA. Translation: AAH69769.1.

PIR

S27016.

RefSeq

NP_001917.1.

UniGene

Hs.711

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZMQ	X-ray	2.10	A/B/C/D	69-100	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q01524.

Proteomic databases

PeptideAtlas

Q01524.

Genome annotation databases

Ensembl

ENSG00000164822. Homo sapiens. [Contig view]

GeneID

1671.

KEGG

hsa:1671.

Organism-specific databases

H-InvDB

HIX0034354.

HGNC

HGNC:2765. DEFA6.

MIM

600471. gene.

PharmGKB

PA27242.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q01524.

HOVERGEN

Q01524.

Gene expression databases

ArrayExpress

Q01524.

CleanEx

HS_DEF6.
HS_DEFA6.

GermOnline

ENSG00000164822. Homo sapiens.

Family and domain databases

InterPro

IPR016327. Alpha-defensin.
IPR006081. Defensin_alpha.
IPR006080. Defensin_mammal.
IPR002366. Defensin_propep.
[Graphical view]

Pfam

PF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]

PIRSF

PIRSF001875. Alpha-defensin. 1 hit.

SMART

SM00048. DEFSN. 1 hit.
[Graphical view]

PROSITE

PS00269. DEFENSIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

Q01524.

NextBio

6876.

SOURCE

Search...

Entry information

Entry name

DEF6_HUMAN

Accession

Primary (citable) accession number: Q01524

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 25, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)