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Protein

Defensin-5

Gene

DEFA5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.3 Publications

GO - Biological processi

  • antibacterial humoral response Source: UniProtKB
  • defense response to fungus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB
  • killing of cells of other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin, Fungicide

Enzyme and pathway databases

ReactomeiREACT_115574. Alpha-defensins.
REACT_115846. Defensins.

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-5
Alternative name(s):
Defensin, alpha 5
HD5(20-94)
Cleaved into the following 4 chains:
Gene namesi
Name:DEFA5
Synonyms:DEF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:2764. DEFA5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27241.

Polymorphism and mutation databases

BioMutaiDEFA5.
DMDMi399353.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Peptidei20 – 9475Defensin-5PRO_0000006787Add
BLAST
Peptidei23 – 9472HD5(23-94)PRO_0000417387Add
BLAST
Peptidei29 – 9466HD5(29-94)PRO_0000417388Add
BLAST
Peptidei56 – 9439HD5(56-94)PRO_0000417389Add
BLAST
Peptidei63 – 9432HD5(63-94)PRO_0000417390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi65 ↔ 931 Publication
Disulfide bondi67 ↔ 821 Publication
Disulfide bondi72 ↔ 921 Publication

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ01523.
PeptideAtlasiQ01523.
PRIDEiQ01523.

Miscellaneous databases

PMAP-CutDBQ01523.

Expressioni

Tissue specificityi

Paneth cells of the small intestine (at protein level).1 Publication

Gene expression databases

BgeeiQ01523.
CleanExiHS_DEFA5.
GenevisibleiQ01523. HS.

Organism-specific databases

HPAiCAB069919.
HPA015775.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi108034. 1 interaction.
IntActiQ01523. 1 interaction.
STRINGi9606.ENSP00000329890.

Structurei

Secondary structure

1
94
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi65 – 706Combined sources
Beta strandi76 – 827Combined sources
Beta strandi89 – 935Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZMPX-ray1.65A/B/C/D63-94[»]
2LXZNMR-A63-94[»]
2MITNMR-A/B63-94[»]
3I5WX-ray1.63A/B63-94[»]
4E82X-ray1.70A/B63-94[»]
4E83X-ray1.90A/B63-94[»]
4E86X-ray2.75A/B/C/D/E/F/G/H/L63-94[»]
ProteinModelPortaliQ01523.
SMRiQ01523. Positions 63-94.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01523.

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG69207.
GeneTreeiENSGT00730000111183.
HOGENOMiHOG000233351.
HOVERGENiHBG011703.
InParanoidiQ01523.
KOiK05230.
OMAiARATCYC.
OrthoDBiEOG75TMFQ.
PhylomeDBiQ01523.
TreeFamiTF338414.

Family and domain databases

InterProiIPR016327. Alpha-defensin.
IPR006080. Defensin_beta/neutrophil.
IPR002366. Defensin_propep.
IPR006081. Mammalian_defensins.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTIAILAAI LLVALQAQAE SLQERADEAT TQKQSGEDNQ DLAISFAGNG
60 70 80 90
LSALRTSGSQ ARATCYCRTG RCATRESLSG VCEISGRLYR LCCR
Length:94
Mass (Da):10,071
Last modified:July 1, 1993 - v1
Checksum:i19B5B00B6BAF90DA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti71 – 711R → H.
Corresponds to variant rs7839771 [ dbSNP | Ensembl ].
VAR_059245

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97925 Genomic DNA. Translation: AAA35754.1.
AF238378 Genomic DNA. Translation: AAT68886.1.
CH471153 Genomic DNA. Translation: EAW80489.1.
BC069690 mRNA. Translation: AAH69690.1.
BC107079 mRNA. Translation: AAI07080.1.
CCDSiCCDS5963.1.
PIRiA44454.
RefSeqiNP_066290.1. NM_021010.2.
UniGeneiHs.655233.

Genome annotation databases

EnsembliENST00000330590; ENSP00000329890; ENSG00000164816.
GeneIDi1670.
KEGGihsa:1670.
UCSCiuc003wra.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97925 Genomic DNA. Translation: AAA35754.1.
AF238378 Genomic DNA. Translation: AAT68886.1.
CH471153 Genomic DNA. Translation: EAW80489.1.
BC069690 mRNA. Translation: AAH69690.1.
BC107079 mRNA. Translation: AAI07080.1.
CCDSiCCDS5963.1.
PIRiA44454.
RefSeqiNP_066290.1. NM_021010.2.
UniGeneiHs.655233.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZMPX-ray1.65A/B/C/D63-94[»]
2LXZNMR-A63-94[»]
2MITNMR-A/B63-94[»]
3I5WX-ray1.63A/B63-94[»]
4E82X-ray1.70A/B63-94[»]
4E83X-ray1.90A/B63-94[»]
4E86X-ray2.75A/B/C/D/E/F/G/H/L63-94[»]
ProteinModelPortaliQ01523.
SMRiQ01523. Positions 63-94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108034. 1 interaction.
IntActiQ01523. 1 interaction.
STRINGi9606.ENSP00000329890.

Polymorphism and mutation databases

BioMutaiDEFA5.
DMDMi399353.

Proteomic databases

PaxDbiQ01523.
PeptideAtlasiQ01523.
PRIDEiQ01523.

Protocols and materials databases

DNASUi1670.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330590; ENSP00000329890; ENSG00000164816.
GeneIDi1670.
KEGGihsa:1670.
UCSCiuc003wra.1. human.

Organism-specific databases

CTDi1670.
GeneCardsiGC08M006900.
HGNCiHGNC:2764. DEFA5.
HPAiCAB069919.
HPA015775.
MIMi600472. gene.
neXtProtiNX_Q01523.
PharmGKBiPA27241.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG69207.
GeneTreeiENSGT00730000111183.
HOGENOMiHOG000233351.
HOVERGENiHBG011703.
InParanoidiQ01523.
KOiK05230.
OMAiARATCYC.
OrthoDBiEOG75TMFQ.
PhylomeDBiQ01523.
TreeFamiTF338414.

Enzyme and pathway databases

ReactomeiREACT_115574. Alpha-defensins.
REACT_115846. Defensins.

Miscellaneous databases

ChiTaRSiDEFA5. human.
EvolutionaryTraceiQ01523.
GeneWikiiDEFA5.
GenomeRNAii1670.
NextBioi6872.
PMAP-CutDBQ01523.
PROiQ01523.
SOURCEiSearch...

Gene expression databases

BgeeiQ01523.
CleanExiHS_DEFA5.
GenevisibleiQ01523. HS.

Family and domain databases

InterProiIPR016327. Alpha-defensin.
IPR006080. Defensin_beta/neutrophil.
IPR002366. Defensin_propep.
IPR006081. Mammalian_defensins.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Paneth cells of the human small intestine express an antimicrobial peptide gene."
    Jones D.E., Bevins C.L.
    J. Biol. Chem. 267:23216-23225(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Intestine.
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Paneth cell trypsin is the processing enzyme for human defensin-5."
    Ghosh D., Porter E., Shen B., Lee S.K., Wilk D., Drazba J., Yadav S.P., Crabb J.W., Ganz T., Bevins C.L.
    Nat. Immunol. 3:583-590(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, PEPTIDE CHARACTERIZATION, GLYCOSYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Antibacterial activity and specificity of the six human alpha-defensins."
    Ericksen B., Wu Z., Lu W., Lehrer R.I.
    Antimicrob. Agents Chemother. 49:269-275(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Crystal structures of human alpha-defensins HNP4, HD5, and HD6."
    Szyk A., Wu Z., Tucker K., Yang D., Lu W., Lubkowski J.
    Protein Sci. 15:2749-2760(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 63-94, FUNCTION, SUBUNIT, DISULFIDE BONDS.

Entry informationi

Entry nameiDEF5_HUMAN
AccessioniPrimary (citable) accession number: Q01523
Secondary accession number(s): A0JDY6, Q3KNV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 22, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.