Q01519 (COX12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 6B

Alternative name(s):
Cytochrome c oxidase polypeptide VIb

Gene names

Name:	COX12
Ordered Locus Names:	YLR038C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	83 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. This protein may be one of the heme-binding subunits of the oxidase.
Subunit structure	Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 1390 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the cytochrome c oxidase subunit 6B family.

Ontologies

Keywords
Cellular component	Mitochondrion
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial respiratory chain complex IV assembly Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	mitochondrial respiratory chain complex IV Inferred from physical interaction Ref.5. Source: SGD
Molecular function	cytochrome-c oxidase activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.5
	Chain	2 – 83	82	Cytochrome c oxidase subunit 6B		PRO_0000194925
Amino acid modifications
	Modified residue	82	1	Phosphoserine Ref.8
	Disulfide bond	27 ↔ 59		By similarity
	Disulfide bond	37 ↔ 48		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01519 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 57A8FAD93756577E	FASTA	83	9,788
	10 20 30 40 50 60 MADQENSPLH TVGFDARFPQ QNQTKHCWQS YVDYHKCVNM KGEDFAPCKV FWKTYNALCP 70 80 LDWIEKWDDQ REKGIFAGDI NSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of COX12, the nuclear gene for a previously unrecognized subunit of Saccharomyces cerevisiae cytochrome c oxidase." Lamarche A.E., Abate M.I., Chan S.H., Trumpower B.L. J. Biol. Chem. 267:22473-22480(1992) [PubMed: 1331057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8; 11-13 AND 14. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme." Taanman J.-W., Capaldi R.A. J. Biol. Chem. 267:22481-22485(1992) [PubMed: 1331058] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20.
[6]	"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method." Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G. Eur. J. Biochem. 227:296-302(1995) [PubMed: 7851399] [Abstract] Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98332 Genomic DNA. Translation: AAA34510.1. Z73210 Genomic DNA. Translation: CAA97566.1. AY557925 Genomic DNA. Translation: AAS56251.1. BK006945 Genomic DNA. Translation: DAA09357.1.
PIR	S31256.
RefSeq	NP_013139.1. NM_001181925.1.
3D structure databases
ProteinModelPortal	Q01519.
SMR	Q01519. Positions 7-80.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4520N.
IntAct	Q01519. 11 interactions.
MINT	MINT-563973.
STRING	Q01519.
Protein family/group databases
TCDB	3.D.4.8.1. proton-translocating cytochrome oxidase (COX) superfamily.
2D gel databases
UCD-2DPAGE	Q01519.
Proteomic databases
PeptideAtlas	Q01519.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR038C; YLR038C; YLR038C.
GeneID	850727.
KEGG	sce:YLR038C.
NMPDR	fig|4932.3.peg.4130.
Organism-specific databases
SGD	S000004028. COX12.
Phylogenomic databases
eggNOG	fuNOG11673.
GeneTree	EFGT00050000004556.
HOGENOM	HBG330644.
OMA	HRCQKAM.
OrthoDB	EOG437VQN.
Gene expression databases
ArrayExpress	Q01519.
Genevestigator	Q01519.
GermOnline	YLR038C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003213. Cyt_c_oxidase_su6B. [Graphical view]
Gene3D	G3DSA:1.10.10.140. Cyt_c_oxidase_6B. 1 hit.
KO	K02267.
Pfam	PF02297. COX6B. 1 hit. [Graphical view]
PIRSF	PIRSF000278. Cyt_c_oxidase_6B. 1 hit.
SUPFAM	SSF47694. Cyt_c_oxidase_6B. 1 hit.
ProtoNet	Search...
Other
NextBio	966816.

Entry information

Entry name

COX12_YEAST

Accession

Primary (citable) accession number: Q01519
Secondary accession number(s): D6VY41, Q9URA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program