ID   ALFC2_PEA               Reviewed;         349 AA.
AC   Q01517;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   27-MAR-2024, entry version 106.
DE   RecName: Full=Fructose-bisphosphate aldolase 2, chloroplastic;
DE            EC=4.1.2.13;
OS   Pisum sativum (Garden pea) (Lathyrus oleraceus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-349, AND PROTEIN SEQUENCE OF 1-32.
RC   STRAIN=cv. Little Marvel, and cv. Sparkle; TISSUE=Leaf;
RX   PubMed=1524427; DOI=10.1016/0003-9861(92)90112-a;
RA   Razdan K., Heinrikson R.L., Zurcher-Neely H., Morris P.W., Anderson L.E.;
RT   "Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs
RT   coding for two distinct pea chloroplast aldolases.";
RL   Arch. Biochem. Biophys. 298:192-197(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-
CC         phosphate + dihydroxyacetone phosphate; Xref=Rhea:RHEA:14729,
CC         ChEBI:CHEBI:32966, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776; EC=4.1.2.13;
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 4/4.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the class I fructose-bisphosphate aldolase
CC       family. {ECO:0000305}.
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DR   EMBL; M97477; AAA33643.1; -; Genomic_DNA.
DR   PIR; S29048; S29048.
DR   AlphaFoldDB; Q01517; -.
DR   SMR; Q01517; -.
DR   SABIO-RK; Q01517; -.
DR   UniPathway; UPA00109; UER00183.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00948; FBP_aldolase_I_a; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR029768; Aldolase_I_AS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000741; FBA_I.
DR   NCBIfam; NF033379; FrucBisAld_I; 1.
DR   PANTHER; PTHR11627; FRUCTOSE-BISPHOSPHATE ALDOLASE; 1.
DR   PANTHER; PTHR11627:SF16; FRUCTOSE-BISPHOSPHATE ALDOLASE, CHLOROPLASTIC; 1.
DR   Pfam; PF00274; Glycolytic; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
DR   PROSITE; PS00158; ALDOLASE_CLASS_I; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Glycolysis; Lyase; Plastid;
KW   Schiff base.
FT   CHAIN           1..349
FT                   /note="Fructose-bisphosphate aldolase 2, chloroplastic"
FT                   /id="PRO_0000216926"
FT   ACT_SITE        177
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        219
FT                   /note="Schiff-base intermediate with dihydroxyacetone-P"
FT                   /evidence="ECO:0000250"
FT   BINDING         47
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         137
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            349
FT                   /note="Necessary for preference for fructose 1,6-
FT                   bisphosphate over fructose 1-phosphate"
FT                   /evidence="ECO:0000250"
FT   VARIANT         29
FT                   /note="A -> W (in strain: cv. Little Marvel)"
SQ   SEQUENCE   349 AA;  37827 MW;  E50715D246B5D7D1 CRC64;
     GSYADELVKT AKTIASPGRG ILAMDESNAT CGKRLDSIGL ENTEANRQAW RTLLVTVPTL
     GEYISGAILF EETLYQSTVD GRKIVDVLVE QNIIPGIKVD KGLVPLAGSN NESWCQGLDG
     LASRSAAYYQ QGARFAKWRT VVSIPNGPSA LAVKEAAWGL ARYAAISQDN GLVPIVEPEI
     LLDGEHGIDR TFEVAQKVWA EVFYYLAENN VQFEGILLKP SMVTPGAESK DKASPTKVAE
     YTLNLLHRRI PPAVPGIMFL SGGQSEVEAT LNLNAMNKSP NPWHVSFSYA RALQNTALKT
     WGGLPENVKA AQEALLFRAK SNSLAQLGKY IGDGESEEAK KDCCQGYSY
//