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Q01517 (ALFC2_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase 2, chloroplastic

EC=4.1.2.13
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentChloroplast
Plastid
   LigandSchiff base
   Molecular functionLyase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Fructose-bisphosphate aldolase 2, chloroplastic
PRO_0000216926

Sites

Active site1771Proton acceptor By similarity
Active site2191Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site471Substrate By similarity
Binding site1371Substrate By similarity
Site3491Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Natural variations

Natural variant291A → W in strain: cv. Little Marvel.

Sequences

Sequence LengthMass (Da)Tools
Q01517 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: E50715D246B5D7D1

FASTA34937,827
        10         20         30         40         50         60 
GSYADELVKT AKTIASPGRG ILAMDESNAT CGKRLDSIGL ENTEANRQAW RTLLVTVPTL 

        70         80         90        100        110        120 
GEYISGAILF EETLYQSTVD GRKIVDVLVE QNIIPGIKVD KGLVPLAGSN NESWCQGLDG 

       130        140        150        160        170        180 
LASRSAAYYQ QGARFAKWRT VVSIPNGPSA LAVKEAAWGL ARYAAISQDN GLVPIVEPEI 

       190        200        210        220        230        240 
LLDGEHGIDR TFEVAQKVWA EVFYYLAENN VQFEGILLKP SMVTPGAESK DKASPTKVAE 

       250        260        270        280        290        300 
YTLNLLHRRI PPAVPGIMFL SGGQSEVEAT LNLNAMNKSP NPWHVSFSYA RALQNTALKT 

       310        320        330        340 
WGGLPENVKA AQEALLFRAK SNSLAQLGKY IGDGESEEAK KDCCQGYSY 

« Hide

References

[1]"Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases."
Razdan K., Heinrikson R.L., Zurcher-Neely H., Morris P.W., Anderson L.E.
Arch. Biochem. Biophys. 298:192-197(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-349, PROTEIN SEQUENCE OF 1-32.
Strain: cv. Little Marvel and cv. Sparkle.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M97477 Genomic DNA. Translation: AAA33643.1.
PIRS29048.

3D structure databases

ProteinModelPortalQ01517.
SMRQ01517. Positions 2-335.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ01517.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKQ01517.
UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALFC2_PEA
AccessionPrimary (citable) accession number: Q01517
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways