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Protein

Fructose-bisphosphate aldolase 2, chloroplastic

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 471SubstrateBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Active sitei177 – 1771Proton acceptorBy similarity
Active sitei219 – 2191Schiff-base intermediate with dihydroxyacetone-PBy similarity
Sitei349 – 3491Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

SABIO-RKQ01517.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 2, chloroplastic (EC:4.1.2.13)
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Fructose-bisphosphate aldolase 2, chloroplasticPRO_0000216926Add
BLAST

Proteomic databases

PRIDEiQ01517.

Structurei

3D structure databases

ProteinModelPortaliQ01517.
SMRiQ01517. Positions 2-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GSYADELVKT AKTIASPGRG ILAMDESNAT CGKRLDSIGL ENTEANRQAW
60 70 80 90 100
RTLLVTVPTL GEYISGAILF EETLYQSTVD GRKIVDVLVE QNIIPGIKVD
110 120 130 140 150
KGLVPLAGSN NESWCQGLDG LASRSAAYYQ QGARFAKWRT VVSIPNGPSA
160 170 180 190 200
LAVKEAAWGL ARYAAISQDN GLVPIVEPEI LLDGEHGIDR TFEVAQKVWA
210 220 230 240 250
EVFYYLAENN VQFEGILLKP SMVTPGAESK DKASPTKVAE YTLNLLHRRI
260 270 280 290 300
PPAVPGIMFL SGGQSEVEAT LNLNAMNKSP NPWHVSFSYA RALQNTALKT
310 320 330 340
WGGLPENVKA AQEALLFRAK SNSLAQLGKY IGDGESEEAK KDCCQGYSY
Length:349
Mass (Da):37,827
Last modified:February 1, 1994 - v2
Checksum:iE50715D246B5D7D1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291A → W in strain: cv. Little Marvel.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97477 Genomic DNA. Translation: AAA33643.1.
PIRiS29048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97477 Genomic DNA. Translation: AAA33643.1.
PIRiS29048.

3D structure databases

ProteinModelPortaliQ01517.
SMRiQ01517. Positions 2-335.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ01517.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
SABIO-RKQ01517.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases."
    Razdan K., Heinrikson R.L., Zurcher-Neely H., Morris P.W., Anderson L.E.
    Arch. Biochem. Biophys. 298:192-197(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-349, PROTEIN SEQUENCE OF 1-32.
    Strain: cv. Little Marvel and cv. Sparkle.
    Tissue: Leaf.

Entry informationi

Entry nameiALFC2_PEA
AccessioniPrimary (citable) accession number: Q01517
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1994
Last modified: January 7, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.