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Q01517

- ALFC2_PEA

UniProt

Q01517 - ALFC2_PEA

Protein

Fructose-bisphosphate aldolase 2, chloroplastic

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei47 – 471SubstrateBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Active sitei177 – 1771Proton acceptorBy similarity
    Active sitei219 – 2191Schiff-base intermediate with dihydroxyacetone-PBy similarity
    Sitei349 – 3491Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    SABIO-RKQ01517.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase 2, chloroplastic (EC:4.1.2.13)
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Fructose-bisphosphate aldolase 2, chloroplasticPRO_0000216926Add
    BLAST

    Proteomic databases

    PRIDEiQ01517.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01517.
    SMRiQ01517. Positions 2-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01517-1 [UniParc]FASTAAdd to Basket

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    GSYADELVKT AKTIASPGRG ILAMDESNAT CGKRLDSIGL ENTEANRQAW    50
    RTLLVTVPTL GEYISGAILF EETLYQSTVD GRKIVDVLVE QNIIPGIKVD 100
    KGLVPLAGSN NESWCQGLDG LASRSAAYYQ QGARFAKWRT VVSIPNGPSA 150
    LAVKEAAWGL ARYAAISQDN GLVPIVEPEI LLDGEHGIDR TFEVAQKVWA 200
    EVFYYLAENN VQFEGILLKP SMVTPGAESK DKASPTKVAE YTLNLLHRRI 250
    PPAVPGIMFL SGGQSEVEAT LNLNAMNKSP NPWHVSFSYA RALQNTALKT 300
    WGGLPENVKA AQEALLFRAK SNSLAQLGKY IGDGESEEAK KDCCQGYSY 349
    Length:349
    Mass (Da):37,827
    Last modified:February 1, 1994 - v2
    Checksum:iE50715D246B5D7D1
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti29 – 291A → W in strain: cv. Little Marvel.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97477 Genomic DNA. Translation: AAA33643.1.
    PIRiS29048.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97477 Genomic DNA. Translation: AAA33643.1 .
    PIRi S29048.

    3D structure databases

    ProteinModelPortali Q01517.
    SMRi Q01517. Positions 2-335.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q01517.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    SABIO-RK Q01517.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases."
      Razdan K., Heinrikson R.L., Zurcher-Neely H., Morris P.W., Anderson L.E.
      Arch. Biochem. Biophys. 298:192-197(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-349, PROTEIN SEQUENCE OF 1-32.
      Strain: cv. Little Marvel and cv. Sparkle.
      Tissue: Leaf.

    Entry informationi

    Entry nameiALFC2_PEA
    AccessioniPrimary (citable) accession number: Q01517
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3