Reviewed, UniProtKB/Swiss-Prot Q01516 (ALFC1_PEA)

Names and origin

Protein names	Recommended name:     Fructose-bisphosphate aldolase 1, chloroplastic     EC=4.1.2.13
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	356 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 6	›6	Chloroplast Ref.1
	Chain	7 – 356	350	Fructose-bisphosphate aldolase 1, chloroplastic		PRO_0000001110
Sites
	Active site	183	1	Proton acceptor By similarity
	Active site	225	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	53	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity
	Site	356	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity
Natural variations
	Natural variant	35	1	A → W in strain: cv. Little Marvel.
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q01516-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 784A76C196D20941	FASTA	356	38,657
	10 20 30 40 50 60 GLTIRAGSYA DELVKTAKTI ASPGRGILAM DESNATCGKR LASIGLENTE VNRQAWRTLL 70 80 90 100 110 120 VTVPTLGEYI SGAILFEETL YQSTTDGRKI VDVLIEQNII PGIKVDKGLV PLAGSNDESW 130 140 150 160 170 180 CQGLDGLASR SAAYYQQGAR FAKWRTVVSI PNGPSALAVK EAAWGLARYA AISQDNGLVP 190 200 210 220 230 240 IVEPEILLDG EHGIDRTFEV AQKVWAEVFY YLAENNVQFE GILLKPSMVT PGAESKDKAS 250 260 270 280 290 300 PTKVAEYTLN LLHRRIPPAV PGIMFLSGGQ SEVEATLNLN AMNKSPNPWH VSFSYARALQ 310 320 330 340 350 NTALKTWGGL PENVKAAQEA LLFRAKSNSL AQLGKYYGDG ESEEAKKELF VKGYSY

References

[1]

"Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases." Razdan K., Heinrikson R.L., Zurcher-Neely H., Morris P.W., Anderson L.E. Arch. Biochem. Biophys. 298:192-197(1992) [PubMed: 1524427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 7-38. Strain: cv. Little Marvel and cv. Sparkle. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97476 Genomic DNA. Translation: AAA33642.1.
PIR	S29047.
3D structure databases
HSSP	HSSP built from PDB template 1A5C based on UniProtKB P14223.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.1.2.13. 287.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC1_PEA

Accession

Primary (citable) accession number: Q01516

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)