Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q01516

- ALFC1_PEA

UniProt

Q01516 - ALFC1_PEA

Protein

Fructose-bisphosphate aldolase 1, chloroplastic

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531SubstrateBy similarity
    Binding sitei143 – 1431SubstrateBy similarity
    Active sitei183 – 1831Proton acceptorBy similarity
    Active sitei225 – 2251Schiff-base intermediate with dihydroxyacetone-PBy similarity
    Sitei356 – 3561Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    SABIO-RKQ01516.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase 1, chloroplastic (EC:4.1.2.13)
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei‹1 – 6›6Chloroplast1 Publication
    Chaini7 – 356350Fructose-bisphosphate aldolase 1, chloroplasticPRO_0000001110Add
    BLAST

    Proteomic databases

    PRIDEiQ01516.
    ProMEXiQ01516.

    Structurei

    3D structure databases

    ProteinModelPortaliQ01516.
    SMRiQ01516. Positions 7-341.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01516-1 [UniParc]FASTAAdd to Basket

    « Hide

    GLTIRAGSYA DELVKTAKTI ASPGRGILAM DESNATCGKR LASIGLENTE    50
    VNRQAWRTLL VTVPTLGEYI SGAILFEETL YQSTTDGRKI VDVLIEQNII 100
    PGIKVDKGLV PLAGSNDESW CQGLDGLASR SAAYYQQGAR FAKWRTVVSI 150
    PNGPSALAVK EAAWGLARYA AISQDNGLVP IVEPEILLDG EHGIDRTFEV 200
    AQKVWAEVFY YLAENNVQFE GILLKPSMVT PGAESKDKAS PTKVAEYTLN 250
    LLHRRIPPAV PGIMFLSGGQ SEVEATLNLN AMNKSPNPWH VSFSYARALQ 300
    NTALKTWGGL PENVKAAQEA LLFRAKSNSL AQLGKYYGDG ESEEAKKELF 350
    VKGYSY 356
    Length:356
    Mass (Da):38,657
    Last modified:July 1, 1993 - v1
    Checksum:i784A76C196D20941
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti35 – 351A → W in strain: cv. Little Marvel.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97476 Genomic DNA. Translation: AAA33642.1.
    PIRiS29047.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97476 Genomic DNA. Translation: AAA33642.1 .
    PIRi S29047.

    3D structure databases

    ProteinModelPortali Q01516.
    SMRi Q01516. Positions 7-341.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q01516.
    ProMEXi Q01516.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    SABIO-RK Q01516.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases."
      Razdan K., Heinrikson R.L., Zurcher-Neely H., Morris P.W., Anderson L.E.
      Arch. Biochem. Biophys. 298:192-197(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 7-38.
      Strain: cv. Little Marvel and cv. Sparkle.
      Tissue: Leaf.

    Entry informationi

    Entry nameiALFC1_PEA
    AccessioniPrimary (citable) accession number: Q01516
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3