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Protein

Fructose-bisphosphate aldolase 1, chloroplastic

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateBy similarity
Binding sitei143 – 1431SubstrateBy similarity
Active sitei183 – 1831Proton acceptorBy similarity
Active sitei225 – 2251Schiff-base intermediate with dihydroxyacetone-PBy similarity
Sitei356 – 3561Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

SABIO-RKQ01516.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 1, chloroplastic (EC:4.1.2.13)
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 6›6Chloroplast1 Publication
Chaini7 – 356350Fructose-bisphosphate aldolase 1, chloroplasticPRO_0000001110Add
BLAST

Proteomic databases

PRIDEiQ01516.

Structurei

3D structure databases

ProteinModelPortaliQ01516.
SMRiQ01516. Positions 7-341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GLTIRAGSYA DELVKTAKTI ASPGRGILAM DESNATCGKR LASIGLENTE
60 70 80 90 100
VNRQAWRTLL VTVPTLGEYI SGAILFEETL YQSTTDGRKI VDVLIEQNII
110 120 130 140 150
PGIKVDKGLV PLAGSNDESW CQGLDGLASR SAAYYQQGAR FAKWRTVVSI
160 170 180 190 200
PNGPSALAVK EAAWGLARYA AISQDNGLVP IVEPEILLDG EHGIDRTFEV
210 220 230 240 250
AQKVWAEVFY YLAENNVQFE GILLKPSMVT PGAESKDKAS PTKVAEYTLN
260 270 280 290 300
LLHRRIPPAV PGIMFLSGGQ SEVEATLNLN AMNKSPNPWH VSFSYARALQ
310 320 330 340 350
NTALKTWGGL PENVKAAQEA LLFRAKSNSL AQLGKYYGDG ESEEAKKELF

VKGYSY
Length:356
Mass (Da):38,657
Last modified:July 1, 1993 - v1
Checksum:i784A76C196D20941
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti35 – 351A → W in strain: cv. Little Marvel.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97476 Genomic DNA. Translation: AAA33642.1.
PIRiS29047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97476 Genomic DNA. Translation: AAA33642.1.
PIRiS29047.

3D structure databases

ProteinModelPortaliQ01516.
SMRiQ01516. Positions 7-341.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ01516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
SABIO-RKQ01516.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases."
    Razdan K., Heinrikson R.L., Zurcher-Neely H., Morris P.W., Anderson L.E.
    Arch. Biochem. Biophys. 298:192-197(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 7-38.
    Strain: cv. Little Marvel and cv. Sparkle.
    Tissue: Leaf.

Entry informationi

Entry nameiALFC1_PEA
AccessioniPrimary (citable) accession number: Q01516
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.