Q01511 (MTC1_PBCVC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase CviBI
Short name=M.CviBI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase CviBI

Gene names

Name:

CVIBIM

Organism

Paramecium bursaria Chlorella virus NC1A (PBCV-NC1A)

Taxonomic identifier

46020 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus

Virus host

Chlorella [TaxID: 3071]

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the CviBI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Modification methylase CviBI		PRO_0000087947
Sites
	Binding site	7	1	S-adenosyl-L-methionine By similarity
	Binding site	11	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	177	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01511 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 7AB57C5089EDD039	FASTA	260	30,705
	10 20 30 40 50 60 MKPIVKWSGG KTDELKRFED YIPSDCSTFI EPFAGGAATF FHVGNQFENK VLSDVHVELV 70 80 90 100 110 120 ALYRAIANGK SQAIYDFMKS HANDEKTYYE VRSWKPEDYV DVASRFYYLR KTCFRGMMRY 130 140 150 160 170 180 NKNGGFNVPF GRYKTYNFED IINEEYYNIL KDTIILEKSF DYIFETYNDS SNFVFLDPPY 190 200 210 220 230 240 DSVFTDYGYC SFGKEEHVRL SNFFKTTKNK CLMVIGATDF IRELYDGYIH TEYEKKYRFK 250 260 LHSGRVGDEI NTTHLVIKNY

References

[1]	"Cloning, sequencing, overproduction, and purification of M. CviBI (GANTC) methyltransferase from Chlorella virus NC-1A." Kan T.N., Li L., Chandrasegaran S. Gene 121:1-7(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96366 Genomic DNA. Translation: AAA88829.1.
3D structure databases
ProteinModelPortal	Q01511.
ModBase	Search...
Protein family/group databases
REBASE	3352. M.CviBI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1020.10. 1 hit.
InterPro	IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012263. M_m6A_EcoRV. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PIRSF	PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC1_PBCVC

Accession

Primary (citable) accession number: Q01511

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program