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Q01511 (MTC1_PBCVC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase CviBI

Short name=M.CviBI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase CviBI
Gene names
Name:CVIBIM
OrganismParamecium bursaria Chlorella virus NC1A (PBCV-NC1A)
Taxonomic identifier46020 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostChlorella [TaxID: 3071]

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the CviBI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260Modification methylase CviBI
PRO_0000087947

Sites

Binding site71S-adenosyl-L-methionine By similarity
Binding site111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site541S-adenosyl-L-methionine By similarity
Binding site1771S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q01511 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 7AB57C5089EDD039

FASTA26030,705
        10         20         30         40         50         60 
MKPIVKWSGG KTDELKRFED YIPSDCSTFI EPFAGGAATF FHVGNQFENK VLSDVHVELV 

        70         80         90        100        110        120 
ALYRAIANGK SQAIYDFMKS HANDEKTYYE VRSWKPEDYV DVASRFYYLR KTCFRGMMRY 

       130        140        150        160        170        180 
NKNGGFNVPF GRYKTYNFED IINEEYYNIL KDTIILEKSF DYIFETYNDS SNFVFLDPPY 

       190        200        210        220        230        240 
DSVFTDYGYC SFGKEEHVRL SNFFKTTKNK CLMVIGATDF IRELYDGYIH TEYEKKYRFK 

       250        260 
LHSGRVGDEI NTTHLVIKNY 

« Hide

References

[1]"Cloning, sequencing, overproduction, and purification of M. CviBI (GANTC) methyltransferase from Chlorella virus NC-1A."
Kan T.N., Li L., Chandrasegaran S.
Gene 121:1-7(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96366 Genomic DNA. Translation: AAA88829.1.

3D structure databases

ProteinModelPortalQ01511.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3352. M.CviBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTC1_PBCVC
AccessionPrimary (citable) accession number: Q01511
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries