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Q01511

- MTC1_PBCVC

UniProt

Q01511 - MTC1_PBCVC

Protein

Modification methylase CviBI

Gene

CVIBIM

Organism
Paramecium bursaria Chlorella virus NC1A (PBCV-NC1A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the CviBI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71S-adenosyl-L-methionineBy similarity
    Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
    Binding sitei177 – 1771S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3352. M.CviBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase CviBI (EC:2.1.1.72)
    Short name:
    M.CviBI
    Alternative name(s):
    Adenine-specific methyltransferase CviBI
    Gene namesi
    Name:CVIBIM
    OrganismiParamecium bursaria Chlorella virus NC1A (PBCV-NC1A)
    Taxonomic identifieri46020 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
    Virus hostiChlorella [TaxID: 3071]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260Modification methylase CviBIPRO_0000087947Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ01511.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q01511-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPIVKWSGG KTDELKRFED YIPSDCSTFI EPFAGGAATF FHVGNQFENK    50
    VLSDVHVELV ALYRAIANGK SQAIYDFMKS HANDEKTYYE VRSWKPEDYV 100
    DVASRFYYLR KTCFRGMMRY NKNGGFNVPF GRYKTYNFED IINEEYYNIL 150
    KDTIILEKSF DYIFETYNDS SNFVFLDPPY DSVFTDYGYC SFGKEEHVRL 200
    SNFFKTTKNK CLMVIGATDF IRELYDGYIH TEYEKKYRFK LHSGRVGDEI 250
    NTTHLVIKNY 260
    Length:260
    Mass (Da):30,705
    Last modified:April 1, 1993 - v1
    Checksum:i7AB57C5089EDD039
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96366 Genomic DNA. Translation: AAA88829.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96366 Genomic DNA. Translation: AAA88829.1 .

    3D structure databases

    ProteinModelPortali Q01511.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3352. M.CviBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, overproduction, and purification of M. CviBI (GANTC) methyltransferase from Chlorella virus NC-1A."
      Kan T.N., Li L., Chandrasegaran S.
      Gene 121:1-7(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTC1_PBCVC
    AccessioniPrimary (citable) accession number: Q01511
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3