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Q01511

- MTC1_PBCVC

UniProt

Q01511 - MTC1_PBCVC

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Protein

Modification methylase CviBI

Gene

CVIBIM

Organism
Paramecium bursaria Chlorella virus NC1A (PBCV-NC1A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the CviBI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71S-adenosyl-L-methionineBy similarity
Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
Binding sitei177 – 1771S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3352. M.CviBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase CviBI (EC:2.1.1.72)
Short name:
M.CviBI
Alternative name(s):
Adenine-specific methyltransferase CviBI
Gene namesi
Name:CVIBIM
OrganismiParamecium bursaria Chlorella virus NC1A (PBCV-NC1A)
Taxonomic identifieri46020 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 3071]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Modification methylase CviBIPRO_0000087947Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ01511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01511 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPIVKWSGG KTDELKRFED YIPSDCSTFI EPFAGGAATF FHVGNQFENK
60 70 80 90 100
VLSDVHVELV ALYRAIANGK SQAIYDFMKS HANDEKTYYE VRSWKPEDYV
110 120 130 140 150
DVASRFYYLR KTCFRGMMRY NKNGGFNVPF GRYKTYNFED IINEEYYNIL
160 170 180 190 200
KDTIILEKSF DYIFETYNDS SNFVFLDPPY DSVFTDYGYC SFGKEEHVRL
210 220 230 240 250
SNFFKTTKNK CLMVIGATDF IRELYDGYIH TEYEKKYRFK LHSGRVGDEI
260
NTTHLVIKNY
Length:260
Mass (Da):30,705
Last modified:April 1, 1993 - v1
Checksum:i7AB57C5089EDD039
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96366 Genomic DNA. Translation: AAA88829.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96366 Genomic DNA. Translation: AAA88829.1 .

3D structure databases

ProteinModelPortali Q01511.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3352. M.CviBI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequencing, overproduction, and purification of M. CviBI (GANTC) methyltransferase from Chlorella virus NC-1A."
    Kan T.N., Li L., Chandrasegaran S.
    Gene 121:1-7(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTC1_PBCVC
AccessioniPrimary (citable) accession number: Q01511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3