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Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

UBP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin. Removes ubiquitin chains that initiate proteolysis of FZO1 and inhibit mitochondrial fusion.2 Publications

Miscellaneous

Present with 2420 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei745NucleophilePROSITE-ProRule annotation1
Active sitei1209Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  • protein deubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
  • ubiquitin recycling Source: SGD

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33651-MONOMER

Protein family/group databases

MEROPSiC19.003

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene namesi
Name:UBP2
Ordered Locus Names:YOR124C
ORF Names:O3281, YOR3281C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR124C
SGDiS000005650 UBP2

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805861 – 1272Ubiquitin carboxyl-terminal hydrolase 2Add BLAST1272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei907PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01476
PaxDbiQ01476
PRIDEiQ01476

PTM databases

iPTMnetiQ01476

Interactioni

Subunit structurei

Forms a ternary complex with RSP5 and RUP1. Interacts with FZO1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RUP1Q122423EBI-19826,EBI-38794

Protein-protein interaction databases

BioGridi34519, 140 interactors
DIPiDIP-2647N
IntActiQ01476, 10 interactors
MINTiQ01476
STRINGi4932.YOR124C

Structurei

3D structure databases

ProteinModelPortaliQ01476
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini736 – 1258USPAdd BLAST523

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

HOGENOMiHOG000094458
InParanoidiQ01476
KOiK11849
OMAiFRKYNDE
OrthoDBiEOG092C04TL

Family and domain databases

InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR025305 UCH_repeat_domain
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF13446 RPT, 3 hits
PF00443 UCH, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q01476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNEDNELQK AIENHHNQLL NQDKENADRN GSVIEDLPLY GTSINQQSTP
60 70 80 90 100
GDVDDGKHLL YPDIATNLPL KTSDRLLDDI LCDTIFLNST DPKVMQKGLQ
110 120 130 140 150
SRGILKESML SYSTFRSSIR PNCLGSLTDQ VVFQTKSEYD SISCPKYNKI
160 170 180 190 200
HVFQAVIFNP SLAEQQISTF DDIVKIPIYH LKVSVKVRQE LERLKKHVGV
210 220 230 240 250
TQFHSLDHLH EYDRVDLSTF DSSDPNLLDY GIYVSDDTNK LILIEIFKPE
260 270 280 290 300
FNSPEEHESF TADAIKKRYN AMCVKNESLD KSETPSQVDC FYTLFKIFKG
310 320 330 340 350
PLTRKSKAEP TKTIDSGNLA LNTHLNPEWL TSKYGFQASS EIDEETNEIF
360 370 380 390 400
TEYVPPDMVD YVNDLETRKI RESFVRKCLQ LIFWGQLSTS LLAPNSPLKN
410 420 430 440 450
TKSVKGMSSL QTSFSTLPWF HLLGESRARI LLNSNEQTHS PLDAEPHFIN
460 470 480 490 500
LSVSHYYTDR DIIRNYESLS SLDPENIGLY FDALTYIANR KGAYQLIAYC
510 520 530 540 550
GKQDIIGQEA LENALLMFKI NPKECNISEL NEATLLSIYK YETSNKSQVT
560 570 580 590 600
SNHLTNLKNA LRLLAKYTKS DKLKFYVDHE PYRALSQAYD TLSIDESVDE
610 620 630 640 650
DIIKTAYSVK INDSPGLKLD CDRALYTIAI SKRSLDLFNF LTEECPQFSN
660 670 680 690 700
YYGPEKLDYQ EALKLLQVNE NASDETILKI FKQKWFDENV YEPDQFLILR
710 720 730 740 750
AALTKISIER NSTLITNFLL TGTIDPNSLP PENWPTGINN IGNTCYLNSL
760 770 780 790 800
LQYYFSIAPL RRYVLEYQKT VENFNDHLSN SGHIRRIGGR EISRGEVERS
810 820 830 840 850
IQFIYQLRNL FYAMVHTRER CVTPSKELAY LAFAPSNVEV EFEVEGNKVV
860 870 880 890 900
DQTGVLSDSK KETTDDAFTT KIKDTSLIDL EMEDGLNGDV GTDANRKKNE
910 920 930 940 950
SNDAEVSENE DTTGLTSPTR VAKISSDQLE NALEMGRQQD VTECIGNVLF
960 970 980 990 1000
QIESGSEPIR YDEDNEQYDL VKQLFYGTTK QSIVPLSATN KVRTKVERFL
1010 1020 1030 1040 1050
SLLINIGDHP KDIYDAFDSY FKDEYLTMEE YGDVIRTVAV TTFPTILQVQ
1060 1070 1080 1090 1100
IQRVYYDRER LMPFKSIEPL PFKEVIYMDR YADTENPLLL AKKKETEEMK
1110 1120 1130 1140 1150
QKLKVMKNRQ RELLSRDDSG LTRKDAFLES IKLLESDTIK KTPLKIEAAN
1160 1170 1180 1190 1200
DVIKTLRNNV QNIDNELMKL YNDINSLEEK ISHQFDDFKE YGYSLFSVFI
1210 1220 1230 1240 1250
HRGEASYGHY WIYIKDRNRN GIWRKYNDET ISEVQEEEVF NFNEGNTATP
1260 1270
YFLVYVKQGQ EGDIEPLKRI LK
Length:1,272
Mass (Da):146,355
Last modified:November 1, 1997 - v2
Checksum:i6D106539AE5C5F3F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti658 – 665Missing (PubMed:1429680).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94916 Genomic DNA Translation: AAA35190.1
X94335 Genomic DNA Translation: CAA64043.1
X90518 Genomic DNA Translation: CAA62120.1
Z75032 Genomic DNA Translation: CAA99323.1
BK006948 Genomic DNA Translation: DAA10898.1
PIRiS60999
RefSeqiNP_014767.3, NM_001183543.3

Genome annotation databases

EnsemblFungiiYOR124C; YOR124C; YOR124C
GeneIDi854291
KEGGisce:YOR124C

Similar proteinsi

Entry informationi

Entry nameiUBP2_YEAST
AccessioniPrimary (citable) accession number: Q01476
Secondary accession number(s): D6W2I2, Q99357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Protein nomenclature information
    List of publications dealing with protein nomenclature and links to specialized websites

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