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UniProtKB/Swiss-Prot Q01468 (4OT1_PSEPU)
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March 2, 2010.
Version 73.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide
| Protein names | Recommended name: 4-oxalocrotonate tautomerase Short name=4-OT EC=5.3.2.- | ||
| Gene names |
| ||
| Encoded on | Plasmid TOL pWW0 | ||
| Organism | Pseudomonas putida | ||
| Taxonomic identifier | 303 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
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Protein attributesHide
| Sequence length | 63 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
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General annotation (Comments)Hide
| Function | Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate. |
| Pathway | |
| Subunit structure | Homohexamer. |
| Sequence similarities | Belongs to the 4-oxalocrotonate tautomerase family. |
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OntologiesHide
| Keywords | |
|---|---|
| Biological process | Aromatic hydrocarbons catabolism |
| Molecular function | Isomerase |
| Technical term | 3D-structure Direct protein sequencing Plasmid |
| Gene Ontology (GO) | |
| Biological process | aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
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Sequence annotation (Features)Hide
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||
Molecule processing | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.1 | |||||||||||||||||||
| Chain | 2 – 63 | 62 | 4-oxalocrotonate tautomerase | PRO_0000209514 | ||||||||||||||||||
Sites | ||||||||||||||||||||||
| Active site | 2 | 1 | Proton acceptor; via imino nitrogen Ref.6 | |||||||||||||||||||
Secondary structure | ||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||
| Beta strand | 3 – 10 | 8 | ||||||||||||||||||||
| Helix | 14 – 32 | 19 | ||||||||||||||||||||
| Helix | 36 – 38 | 3 | ||||||||||||||||||||
| Beta strand | 40 – 46 | 7 | ||||||||||||||||||||
| Helix | 48 – 50 | 3 | ||||||||||||||||||||
| Beta strand | 51 – 53 | 3 | ||||||||||||||||||||
| Helix | 58 – 60 | 3 | ||||||||||||||||||||
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ReferencesHide
| [1] | "4-oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer." Chen L.H., Kenyon G.L., Curtin F., Harayama S., Bembenek M.E., Hajipour G., Whitman C.P. J. Biol. Chem. 267:17716-17721(1992) [PubMed: 1339435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-34. Strain: ATCC 33015 / mt-2 / JCM 6156. |
| [2] | "Comparison of the nucleotide sequences of the meta-cleavage pathway genes of TOL plasmid pWW0 from Pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution." Harayama S., Rekik M. Mol. Gen. Genet. 239:81-89(1993) [PubMed: 8510667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33015 / mt-2 / JCM 6156. |
| [3] | "Complete sequence of the IncP-9 TOL plasmid pWW0 from Pseudomonas putida." Greated A., Lambertsen L., Williams P.A., Thomas C.M. Environ. Microbiol. 4:856-871(2002) [PubMed: 12534468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases." Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B. Biochemistry 35:792-802(1996) [PubMed: 8547259] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). |
| [5] | "Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2-oxo-3-pentynoate at 2.4-A resolution: analysis and implications for the mechanism of inactivation and catalysis." Taylor A.B., Czerwinski R.M., Johnson W.H. Jr., Whitman C.P., Hackert M.L. Biochemistry 37:14692-14700(1998) [PubMed: 9778344] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). Strain: ATCC 33015 / mt-2 / JCM 6156. |
| [6] | "Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies." Stivers J.T., Abeygunawardana C., Mildvan A.S., Hajipour G., Whitman C.P., Chen L.H. Biochemistry 35:803-813(1996) [PubMed: 8547260] [Abstract] Cited for: STRUCTURE BY NMR, ACTIVE SITE. |
| + | Additional computationally mapped references. |
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Cross-referencesHide
Sequence databases | |||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | M95650 Genomic DNA. Translation: AAA26046.1. M94186 Genomic DNA. Translation: AAA25694.1. AJ344068 Genomic DNA. Translation: CAC86799.1. | ||||||||||||||||||||||||||||||||||||
| PIR | A43397. | ||||||||||||||||||||||||||||||||||||
| RefSeq | NP_542859.1. | ||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ModBase | Search... | ||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||
| GeneID | 1218749. | ||||||||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||||||||
| ProtClustDB | CLSK899099. | ||||||||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||||||||
| BioCyc | MetaCyc:MONOMER-12750. | ||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||
| InterPro | IPR004370. 4-oxalocrotonate_tautomerase. IPR018191. Oxalocrotonate_tautomerase_sub. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| Pfam | PF01361. Tautomerase. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| TIGRFAMs | TIGR00013. taut. 1 hit. | ||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||
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Entry informationHide
| Entry name | 4OT1_PSEPU | ||||||||
| Accession | Primary (citable) accession number: Q01468 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
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Relevant documentsHide
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
