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Q01468

- 4OT1_PSEPU

UniProt

Q01468 - 4OT1_PSEPU

Protein

2-hydroxymuconate tautomerase

Gene

xylH

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.1 Publication

    Catalytic activityi

    (2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Proton acceptor; via imino nitrogen1 Publication

    GO - Molecular functioni

    1. isomerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. toluene catabolic process Source: UniProtKB-UniPathway
    2. xylene catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12750.
    RETL1328306-WGS:GSTH-5985-MONOMER.
    UniPathwayiUPA00228.
    UPA00273.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-hydroxymuconate tautomerase (EC:5.3.2.6)
    Alternative name(s):
    4-oxalocrotonate tautomerase
    Short name:
    4-OT
    Gene namesi
    Name:xylH
    Encoded oniPlasmid TOL pWW00 Publication
    OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
    Taxonomic identifieri303 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 63622-hydroxymuconate tautomerasePRO_0000209514Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    Secondary structure

    1
    63
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 108
    Helixi14 – 3219
    Helixi36 – 383
    Beta strandi40 – 467
    Helixi48 – 503
    Helixi58 – 603

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BJPX-ray2.40A/B/C/D/E2-63[»]
    2FM7X-ray2.80A/B/C/D/E/F2-62[»]
    4OTAX-ray2.75A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R2-63[»]
    4OTBX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L2-63[»]
    4OTCX-ray2.28A/B/C/D/E/F/G/H/I2-63[»]
    ProteinModelPortaliQ01468.
    SMRiQ01468. Positions 2-63.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ01468.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR004370. 4-oxalocrotonate_tautomerase.
    IPR014347. Tautomerase/MIF_sf.
    IPR018191. Tautomerase_Pseudo-typ.
    [Graphical view]
    PfamiPF01361. Tautomerase. 1 hit.
    [Graphical view]
    SUPFAMiSSF55331. SSF55331. 1 hit.
    TIGRFAMsiTIGR00013. taut. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q01468-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIAQIHILE GRSDEQKETL IREVSEAISR SLDAPLTSVR VIITEMAKGH   50
    FGIGGELASK VRR 63
    Length:63
    Mass (Da):6,942
    Last modified:January 23, 2007 - v2
    Checksum:i23804AB94A126802
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95650 Genomic DNA. Translation: AAA26046.1.
    M94186 Genomic DNA. Translation: AAA25694.1.
    AJ344068 Genomic DNA. Translation: CAC86799.1.
    PIRiA43397.
    RefSeqiNP_542859.1. NC_003350.1.
    WP_011005902.1. NC_003350.1.

    Genome annotation databases

    GeneIDi1218749.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95650 Genomic DNA. Translation: AAA26046.1 .
    M94186 Genomic DNA. Translation: AAA25694.1 .
    AJ344068 Genomic DNA. Translation: CAC86799.1 .
    PIRi A43397.
    RefSeqi NP_542859.1. NC_003350.1.
    WP_011005902.1. NC_003350.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BJP X-ray 2.40 A/B/C/D/E 2-63 [» ]
    2FM7 X-ray 2.80 A/B/C/D/E/F 2-62 [» ]
    4OTA X-ray 2.75 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R 2-63 [» ]
    4OTB X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L 2-63 [» ]
    4OTC X-ray 2.28 A/B/C/D/E/F/G/H/I 2-63 [» ]
    ProteinModelPortali Q01468.
    SMRi Q01468. Positions 2-63.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1218749.

    Enzyme and pathway databases

    UniPathwayi UPA00228 .
    UPA00273 .
    BioCyci MetaCyc:MONOMER-12750.
    RETL1328306-WGS:GSTH-5985-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q01468.

    Family and domain databases

    InterProi IPR004370. 4-oxalocrotonate_tautomerase.
    IPR014347. Tautomerase/MIF_sf.
    IPR018191. Tautomerase_Pseudo-typ.
    [Graphical view ]
    Pfami PF01361. Tautomerase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55331. SSF55331. 1 hit.
    TIGRFAMsi TIGR00013. taut. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "4-oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer."
      Chen L.H., Kenyon G.L., Curtin F., Harayama S., Bembenek M.E., Hajipour G., Whitman C.P.
      J. Biol. Chem. 267:17716-17721(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-34, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
    2. "Comparison of the nucleotide sequences of the meta-cleavage pathway genes of TOL plasmid pWW0 from Pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution."
      Harayama S., Rekik M.
      Mol. Gen. Genet. 239:81-89(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
    3. "Complete sequence of the IncP-9 TOL plasmid pWW0 from Pseudomonas putida."
      Greated A., Lambertsen L., Williams P.A., Thomas C.M.
      Environ. Microbiol. 4:856-871(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases."
      Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B.
      Biochemistry 35:792-802(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, REACTION MECHANISM.
    5. "Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2-oxo-3-pentynoate at 2.4-A resolution: analysis and implications for the mechanism of inactivation and catalysis."
      Taylor A.B., Czerwinski R.M., Johnson W.H. Jr., Whitman C.P., Hackert M.L.
      Biochemistry 37:14692-14700(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
      Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
    6. "Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies."
      Stivers J.T., Abeygunawardana C., Mildvan A.S., Hajipour G., Whitman C.P., Chen L.H.
      Biochemistry 35:803-813(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, ACTIVE SITE.

    Entry informationi

    Entry namei4OT1_PSEPU
    AccessioniPrimary (citable) accession number: Q01468
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3