Q01468 (4OT1_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-hydroxymuconate tautomerase
EC=5.3.2.6

Alternative name(s):
4-oxalocrotonate tautomerase
Short name=4-OT

Gene names

Name:

xylH

Encoded on

Plasmid TOL pWW0

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	63 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate. Ref.1
Catalytic activity	(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate. Ref.1
Pathway	Xenobiotic degradation; toluene degradation. Xenobiotic degradation; xylene degradation.
Subunit structure	Homohexamer. Ref.4
Sequence similarities	Belongs to the 4-oxalocrotonate tautomerase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Isomerase
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	toluene catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway xylene catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 63

62

2-hydroxymuconate tautomerase

PRO_0000209514

Sites

Active site

2

1

Proton acceptor; via imino nitrogen Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

63

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Helix

14 – 32

19

Helix

36 – 38

3

Beta strand

40 – 46

7

Helix

48 – 50

3

Helix

58 – 60

3

Sequences

	Sequence		Length	Mass (Da)
	Q01468 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 23804AB94A126802	FASTA	63	6,942
	10 20 30 40 50 60 MPIAQIHILE GRSDEQKETL IREVSEAISR SLDAPLTSVR VIITEMAKGH FGIGGELASK VRR

References

[1]	"4-oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer." Chen L.H., Kenyon G.L., Curtin F., Harayama S., Bembenek M.E., Hajipour G., Whitman C.P. J. Biol. Chem. 267:17716-17721(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-34, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
[2]	"Comparison of the nucleotide sequences of the meta-cleavage pathway genes of TOL plasmid pWW0 from Pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution." Harayama S., Rekik M. Mol. Gen. Genet. 239:81-89(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
[3]	"Complete sequence of the IncP-9 TOL plasmid pWW0 from Pseudomonas putida." Greated A., Lambertsen L., Williams P.A., Thomas C.M. Environ. Microbiol. 4:856-871(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases." Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B. Biochemistry 35:792-802(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, REACTION MECHANISM.
[5]	"Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2-oxo-3-pentynoate at 2.4-A resolution: analysis and implications for the mechanism of inactivation and catalysis." Taylor A.B., Czerwinski R.M., Johnson W.H. Jr., Whitman C.P., Hackert M.L. Biochemistry 37:14692-14700(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
[6]	"Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies." Stivers J.T., Abeygunawardana C., Mildvan A.S., Hajipour G., Whitman C.P., Chen L.H. Biochemistry 35:803-813(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, ACTIVE SITE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M95650 Genomic DNA. Translation: AAA26046.1.
M94186 Genomic DNA. Translation: AAA25694.1.
AJ344068 Genomic DNA. Translation: CAC86799.1.

PIR

A43397.

RefSeq

NP_542859.1. NC_003350.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BJP	X-ray	2.40	A/B/C/D/E	2-63	[»]
2FM7	X-ray	2.80	A/B/C/D/E/F	2-62	[»]
4OTA	X-ray	2.75	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R	2-62	[»]
4OTB	X-ray	2.50	A/B/C/D/E/F/G/H/I/J/K/L	2-62	[»]
4OTC	X-ray	2.28	A/B/C/D/E/F/G/H/I	2-62	[»]

ProteinModelPortal

Q01468.

SMR

Q01468. Positions 2-63.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1218749.

Phylogenomic databases

ProtClustDB

CLSK899099.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-12750.

UniPathway

UPA00228.
UPA00273.

Family and domain databases

InterPro

IPR004370. 4-oxalocrotonate_tautomerase.
IPR018191. Oxalocrotonate_taut_bac/arc.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]

Pfam

PF01361. Tautomerase. 1 hit.
[Graphical view]

SUPFAM

SSF55331. SSF55331. 1 hit.

TIGRFAMs

TIGR00013. taut. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q01468.

Entry information

Entry name

4OT1_PSEPU

Accession

Primary (citable) accession number: Q01468

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program