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Protein

2-hydroxymuconate tautomerase

Gene

xylH

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.1 Publication

Catalytic activityi

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate.1 Publication

Pathway:itoluene degradation

This protein is involved in the pathway toluene degradation, which is part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the pathway toluene degradation and in Xenobiotic degradation.

Pathway:ixylene degradation

This protein is involved in the pathway xylene degradation, which is part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the pathway xylene degradation and in Xenobiotic degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogen1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12750.
RETL1328306-WGS:GSTH-5985-MONOMER.
UniPathwayiUPA00228.
UPA00273.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxymuconate tautomerase (EC:5.3.2.6)
Alternative name(s):
4-oxalocrotonate tautomerase
Short name:
4-OT
Gene namesi
Name:xylH
Encoded oniPlasmid TOL pWW00 Publication
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 63622-hydroxymuconate tautomerasePRO_0000209514Add
BLAST

Interactioni

Subunit structurei

Homohexamer.1 Publication

Structurei

Secondary structure

1
63
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi14 – 3219Combined sources
Helixi36 – 383Combined sources
Beta strandi40 – 467Combined sources
Helixi48 – 503Combined sources
Beta strandi51 – 533Combined sources
Helixi58 – 603Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BJPX-ray2.40A/B/C/D/E2-63[»]
2FM7X-ray2.80A/B/C/D/E/F2-62[»]
4OTAX-ray2.75A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R2-63[»]
4OTBX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L2-63[»]
4OTCX-ray2.28A/B/C/D/E/F/G/H/I2-63[»]
4X19X-ray1.94A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d2-63[»]
4X1CX-ray1.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-63[»]
ProteinModelPortaliQ01468.
SMRiQ01468. Positions 2-63.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01468.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK01821.

Family and domain databases

InterProiIPR004370. 4-oxalocrotonate_tautomerase.
IPR014347. Tautomerase/MIF_sf.
IPR018191. Tautomerase_Pseudo-typ.
[Graphical view]
PfamiPF01361. Tautomerase. 1 hit.
[Graphical view]
SUPFAMiSSF55331. SSF55331. 1 hit.
TIGRFAMsiTIGR00013. taut. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIAQIHILE GRSDEQKETL IREVSEAISR SLDAPLTSVR VIITEMAKGH
60
FGIGGELASK VRR
Length:63
Mass (Da):6,942
Last modified:January 23, 2007 - v2
Checksum:i23804AB94A126802
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95650 Genomic DNA. Translation: AAA26046.1.
M94186 Genomic DNA. Translation: AAA25694.1.
AJ344068 Genomic DNA. Translation: CAC86799.1.
PIRiA43397.
RefSeqiNP_542859.1. NC_003350.1.
WP_011005902.1. NC_003350.1.

Genome annotation databases

GeneIDi1218749.
KEGGipg:1218749.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95650 Genomic DNA. Translation: AAA26046.1.
M94186 Genomic DNA. Translation: AAA25694.1.
AJ344068 Genomic DNA. Translation: CAC86799.1.
PIRiA43397.
RefSeqiNP_542859.1. NC_003350.1.
WP_011005902.1. NC_003350.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BJPX-ray2.40A/B/C/D/E2-63[»]
2FM7X-ray2.80A/B/C/D/E/F2-62[»]
4OTAX-ray2.75A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R2-63[»]
4OTBX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L2-63[»]
4OTCX-ray2.28A/B/C/D/E/F/G/H/I2-63[»]
4X19X-ray1.94A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d2-63[»]
4X1CX-ray1.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-63[»]
ProteinModelPortaliQ01468.
SMRiQ01468. Positions 2-63.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1218749.
KEGGipg:1218749.

Phylogenomic databases

KOiK01821.

Enzyme and pathway databases

UniPathwayiUPA00228.
UPA00273.
BioCyciMetaCyc:MONOMER-12750.
RETL1328306-WGS:GSTH-5985-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ01468.

Family and domain databases

InterProiIPR004370. 4-oxalocrotonate_tautomerase.
IPR014347. Tautomerase/MIF_sf.
IPR018191. Tautomerase_Pseudo-typ.
[Graphical view]
PfamiPF01361. Tautomerase. 1 hit.
[Graphical view]
SUPFAMiSSF55331. SSF55331. 1 hit.
TIGRFAMsiTIGR00013. taut. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "4-oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer."
    Chen L.H., Kenyon G.L., Curtin F., Harayama S., Bembenek M.E., Hajipour G., Whitman C.P.
    J. Biol. Chem. 267:17716-17721(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-34, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
  2. "Comparison of the nucleotide sequences of the meta-cleavage pathway genes of TOL plasmid pWW0 from Pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution."
    Harayama S., Rekik M.
    Mol. Gen. Genet. 239:81-89(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
  3. "Complete sequence of the IncP-9 TOL plasmid pWW0 from Pseudomonas putida."
    Greated A., Lambertsen L., Williams P.A., Thomas C.M.
    Environ. Microbiol. 4:856-871(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases."
    Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B.
    Biochemistry 35:792-802(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, REACTION MECHANISM.
  5. "Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2-oxo-3-pentynoate at 2.4-A resolution: analysis and implications for the mechanism of inactivation and catalysis."
    Taylor A.B., Czerwinski R.M., Johnson W.H. Jr., Whitman C.P., Hackert M.L.
    Biochemistry 37:14692-14700(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
    Strain: ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2.
  6. "Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies."
    Stivers J.T., Abeygunawardana C., Mildvan A.S., Hajipour G., Whitman C.P., Chen L.H.
    Biochemistry 35:803-813(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, ACTIVE SITE.

Entry informationi

Entry namei4OT1_PSEPU
AccessioniPrimary (citable) accession number: Q01468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.