Q01462 (LDH1_LACLA) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 104.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: L-lactate dehydrogenase 1 Short name=L-LDH 1 EC=1.1.1.27 | ||||||||
| Gene names |
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| Organism | Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) | ||||||||
| Taxonomic identifier | 272623 [NCBI] | ||||||||
| Taxonomic lineage | Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus |
Protein attributes
| Sequence length | 325 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | (S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488 |
| Enzyme regulation | Fructose-1,6-diphosphate-dependent. HAMAP MF_00488 |
| Pathway | Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488 |
| Subunit structure | Homotetramer By similarity. HAMAP MF_00488 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00488. |
| Sequence similarities | Belongs to the LDH/MDH superfamily. LDH family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 325 | 324 | L-lactate dehydrogenase 1 HAMAP MF_00488 | PRO_0000168351 | |||||
Regions | |||||||||
| Nucleotide binding | 15 – 43 | 29 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 178 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 91 | 1 | Substrate By similarity | ||||||
| Binding site | 123 | 1 | NAD or substrate By similarity | ||||||
| Binding site | 154 | 1 | Substrate By similarity | ||||||
| Binding site | 232 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 223 | 1 | Phosphotyrosine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 270 | 1 | C → F in AAA25172. Ref.1 | ||||||
| Sequence conflict | 270 | 1 | C → F in AAA99896. Ref.1 | ||||||
| Sequence conflict | 270 | 1 | C → F in AAB51678. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning, nucleotide sequence, expression, and chromosomal location of ldh, the gene encoding L-(+)-lactate dehydrogenase, from Lactococcus lactis." Llanos R.M., Hillier A.J., Davidson B.E. J. Bacteriol. 174:6956-6964(1992) [PubMed: 1400245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LM0230. |
| [2] | "Cloning and sequence analysis of the gene encoding L-lactate dehydrogenase from Lactococcus lactis: evolutionary relationships between 21 different LDH enzymes." Griffin H.G., Swindell S.R., Gasson M.G. Gene 122:193-197(1992) [PubMed: 1452029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes." Swindell S.R., Griffin H.G., Gasson M.J. Microbiology 140:1301-1305(1994) [PubMed: 8081494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BU2-60 and IL1403. |
| [4] | "The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes." Urbach E., Daniels B., Salama M.S., Sandine W.E., Giovannoni S.J. Appl. Environ. Microbiol. 63:694-702(1997) [PubMed: 9023947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1117M, 112, BEN121 and BO34. |
| [5] | "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M95919 Genomic DNA. Translation: AAA25172.1. M88490 Genomic DNA. Translation: AAA25187.1. L07920 Genomic DNA. Translation: AAA99896.1. U04315 Genomic DNA. Translation: AAA61963.1. U02386 Genomic DNA. Translation: AAA61962.1. U78632 Genomic DNA. Translation: AAB51676.1. U78636 Genomic DNA. Translation: AAB51680.1. U78637 Genomic DNA. Translation: AAB51681.1. U78638 Genomic DNA. Translation: AAB51682.1. U78634 Genomic DNA. Translation: AAB51678.1. AE005176 Genomic DNA. Translation: AAK05429.1. |
| PIR | A45246. C86791. JN0449. |
| RefSeq | NP_267487.1. NC_002662.1. |
3D structure databases | |
| ProteinModelPortal | Q01462. |
| SMR | Q01462. Positions 1-318. |
| ModBase | Search... |
PTM databases | |
| PhosSite | Q01462. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1114981. |
| GenomeReviews | Gene locus LL1331 in contig AE005176_GR. |
| KEGG | lla:L0017. |
| NMPDR | fig|272623.1.peg.1366. |
| PATRIC | 22295060. VBILacLac136773_1438. |
Phylogenomic databases | |
| HOGENOM | HBG566126. |
| OMA | MDLNHGV. |
| ProtClustDB | PRK00066. |
Enzyme and pathway databases | |
| BioCyc | LLAC272623:L0017-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00488. Lactate_dehydrog. [Tree] |
| InterPro | IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K00016. |
| PANTHER | PTHR11540:SF3. PTHR11540:SF3. 1 hit. |
| Pfam | PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000102. Lac_mal_DH. 1 hit. |
| PRINTS | PR00086. LLDHDRGNASE. |
| SUPFAM | SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit. |
| TIGRFAMs | TIGR01771. L-LDH-NAD. 1 hit. |
| PROSITE | PS00064. L_LDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LDH1_LACLA | ||||||||
| Accession | Primary (citable) accession number: Q01462 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with