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Q01462 (LDH1_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-lactate dehydrogenase 1
Short name=L-LDH 1
EC=1.1.1.27
Gene names
Name:ldhA
Synonyms:ldh
Ordered Locus Names:LL1331
ORF Names:L0017
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488

Enzyme regulation

Fructose-1,6-diphosphate-dependent. HAMAP MF_00488

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488

Subunit structure

Homotetramer By similarity. HAMAP MF_00488

Subcellular location

Cytoplasm By similarity HAMAP MF_00488.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 325324L-lactate dehydrogenase 1 HAMAP MF_00488
PRO_0000168351

Regions

Nucleotide binding15 – 4329NAD By similarity

Sites

Active site1781Proton acceptor By similarity
Binding site911Substrate By similarity
Binding site1231NAD or substrate By similarity
Binding site1541Substrate By similarity
Binding site2321Substrate By similarity

Amino acid modifications

Modified residue2231Phosphotyrosine By similarity

Experimental info

Sequence conflict2701C → F in AAA25172. Ref.1
Sequence conflict2701C → F in AAA99896. Ref.1
Sequence conflict2701C → F in AAB51678. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q01462 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8BFA78343AF13003

FASTA32535,051
        10         20         30         40         50         60 
MADKQRKKVI LVGDGAVGSS YAFALVNQGI AQELGIVDLF KEKTQGDAED LSHALAFTSP 

        70         80         90        100        110        120 
KKIYSADYSD ASDADLVVLT SGAPQKPGET RLDLVEKNLR ITKDVVTKIV ASGFKGIFLV 

       130        140        150        160        170        180 
AANPVDILTY ATWKFSGFPK NRVVGSGTSL DTARFRQALA EKVDVDARSI HAYIMGEHGD 

       190        200        210        220        230        240 
SEFAVWSHAN VAGVKLEQWF QENDYLNEAE IVELFESVRD AAYSIIAKKG ATFYGVAVAL 

       250        260        270        280        290        300 
ARITKAILDD EHAVLPVSVF QDGQYGVSDC YLGQPAVVGA EGVVNPIHIP LNDAEMQKME 

       310        320 
ASGAQLKAII DEAFAKEEFA SAVKN

« Hide

References

« Hide 'large scale' references
[1]"Cloning, nucleotide sequence, expression, and chromosomal location of ldh, the gene encoding L-(+)-lactate dehydrogenase, from Lactococcus lactis."
Llanos R.M., Hillier A.J., Davidson B.E.
J. Bacteriol. 174:6956-6964(1992) [PubMed: 1400245] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LM0230.
[2]"Cloning and sequence analysis of the gene encoding L-lactate dehydrogenase from Lactococcus lactis: evolutionary relationships between 21 different LDH enzymes."
Griffin H.G., Swindell S.R., Gasson M.G.
Gene 122:193-197(1992) [PubMed: 1452029] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes."
Swindell S.R., Griffin H.G., Gasson M.J.
Microbiology 140:1301-1305(1994) [PubMed: 8081494] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BU2-60 and IL1403.
[4]"The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes."
Urbach E., Daniels B., Salama M.S., Sandine W.E., Giovannoni S.J.
Appl. Environ. Microbiol. 63:694-702(1997) [PubMed: 9023947] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1117M, 112, BEN121 and BO34.
[5]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M95919 Genomic DNA. Translation: AAA25172.1.
M88490 Genomic DNA. Translation: AAA25187.1.
L07920 Genomic DNA. Translation: AAA99896.1.
U04315 Genomic DNA. Translation: AAA61963.1.
U02386 Genomic DNA. Translation: AAA61962.1.
U78632 Genomic DNA. Translation: AAB51676.1.
U78636 Genomic DNA. Translation: AAB51680.1.
U78637 Genomic DNA. Translation: AAB51681.1.
U78638 Genomic DNA. Translation: AAB51682.1.
U78634 Genomic DNA. Translation: AAB51678.1.
AE005176 Genomic DNA. Translation: AAK05429.1.
PIRA45246.
C86791.
JN0449.
RefSeqNP_267487.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ01462.
SMRQ01462. Positions 1-318.
ModBaseSearch...

PTM databases

PhosSiteQ01462.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1114981.
GenomeReviewsGene locus LL1331 in contig AE005176_GR.
KEGGlla:L0017.
NMPDRfig|272623.1.peg.1366.
PATRIC22295060. VBILacLac136773_1438.

Phylogenomic databases

HOGENOMHBG566126.
OMAMDLNHGV.
ProtClustDBPRK00066.

Enzyme and pathway databases

BioCycLLAC272623:L0017-MONOMER.

Family and domain databases

HAMAPMF_00488. Lactate_dehydrog.
[Tree]
InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00016.
PANTHERPTHR11540:SF3. PTHR11540:SF3. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLDH1_LACLA
AccessionPrimary (citable) accession number: Q01462
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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