Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase 1

Gene

ldhA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Enzyme regulationi

Fructose-1,6-diphosphate-dependent.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 2 (ldhB), L-lactate dehydrogenase 3 (ldhX), L-lactate dehydrogenase 1 (ldhA)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91SubstrateBy similarity1
Binding sitei123NAD or substrateBy similarity1
Binding sitei154SubstrateBy similarity1
Active sitei178Proton acceptorBy similarity1
Binding sitei232SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 43NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKQ01462.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 1 (EC:1.1.1.27)
Short name:
L-LDH 1
Gene namesi
Name:ldhA
Synonyms:ldh
Ordered Locus Names:LL1331
ORF Names:L0017
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001683512 – 325L-lactate dehydrogenase 1Add BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ01462.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272623.L0017.

Structurei

3D structure databases

ProteinModelPortaliQ01462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiNNEHRVL.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKQRKKVI LVGDGAVGSS YAFALVNQGI AQELGIVDLF KEKTQGDAED
60 70 80 90 100
LSHALAFTSP KKIYSADYSD ASDADLVVLT SGAPQKPGET RLDLVEKNLR
110 120 130 140 150
ITKDVVTKIV ASGFKGIFLV AANPVDILTY ATWKFSGFPK NRVVGSGTSL
160 170 180 190 200
DTARFRQALA EKVDVDARSI HAYIMGEHGD SEFAVWSHAN VAGVKLEQWF
210 220 230 240 250
QENDYLNEAE IVELFESVRD AAYSIIAKKG ATFYGVAVAL ARITKAILDD
260 270 280 290 300
EHAVLPVSVF QDGQYGVSDC YLGQPAVVGA EGVVNPIHIP LNDAEMQKME
310 320
ASGAQLKAII DEAFAKEEFA SAVKN
Length:325
Mass (Da):35,051
Last modified:January 23, 2007 - v3
Checksum:i8BFA78343AF13003
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270C → F in AAA25172 (PubMed:1400245).Curated1
Sequence conflicti270C → F in AAA99896 (PubMed:1400245).Curated1
Sequence conflicti270C → F in AAB51678 (PubMed:9023947).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95919 Genomic DNA. Translation: AAA25172.1.
M88490 Genomic DNA. Translation: AAA25187.1.
L07920 Genomic DNA. Translation: AAA99896.1.
U04315 Genomic DNA. Translation: AAA61963.1.
U02386 Genomic DNA. Translation: AAA61962.1.
U78632 Genomic DNA. Translation: AAB51676.1.
U78636 Genomic DNA. Translation: AAB51680.1.
U78637 Genomic DNA. Translation: AAB51681.1.
U78638 Genomic DNA. Translation: AAB51682.1.
U78634 Genomic DNA. Translation: AAB51678.1.
AE005176 Genomic DNA. Translation: AAK05429.1.
PIRiA45246.
C86791.
JN0449.
RefSeqiNP_267487.1. NC_002662.1.
WP_003131075.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05429; AAK05429; L0017.
GeneIDi1114981.
KEGGilla:L0017.
PATRICi22295060. VBILacLac136773_1438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95919 Genomic DNA. Translation: AAA25172.1.
M88490 Genomic DNA. Translation: AAA25187.1.
L07920 Genomic DNA. Translation: AAA99896.1.
U04315 Genomic DNA. Translation: AAA61963.1.
U02386 Genomic DNA. Translation: AAA61962.1.
U78632 Genomic DNA. Translation: AAB51676.1.
U78636 Genomic DNA. Translation: AAB51680.1.
U78637 Genomic DNA. Translation: AAB51681.1.
U78638 Genomic DNA. Translation: AAB51682.1.
U78634 Genomic DNA. Translation: AAB51678.1.
AE005176 Genomic DNA. Translation: AAK05429.1.
PIRiA45246.
C86791.
JN0449.
RefSeqiNP_267487.1. NC_002662.1.
WP_003131075.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ01462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L0017.

Proteomic databases

PaxDbiQ01462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05429; AAK05429; L0017.
GeneIDi1114981.
KEGGilla:L0017.
PATRICi22295060. VBILacLac136773_1438.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiNNEHRVL.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
SABIO-RKQ01462.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH1_LACLA
AccessioniPrimary (citable) accession number: Q01462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.