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Protein

L-lactate dehydrogenase 1

Gene

ldhA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Enzyme regulationi

Fructose-1,6-diphosphate-dependent.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 2 (ldhB), L-lactate dehydrogenase 3 (ldhX), L-lactate dehydrogenase 1 (ldhA)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91SubstrateBy similarity1
Binding sitei123NAD or substrateBy similarity1
Binding sitei154SubstrateBy similarity1
Active sitei178Proton acceptorBy similarity1
Binding sitei232SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 43NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciLLAC272623:G1FZA-1408-MONOMER
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 1 (EC:1.1.1.27)
Short name:
L-LDH 1
Gene namesi
Name:ldhA
Synonyms:ldh
Ordered Locus Names:LL1331
ORF Names:L0017
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001683512 – 325L-lactate dehydrogenase 1Add BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ01462

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272623.L0017

Structurei

3D structure databases

ProteinModelPortaliQ01462
SMRiQ01462
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKQRKKVI LVGDGAVGSS YAFALVNQGI AQELGIVDLF KEKTQGDAED
60 70 80 90 100
LSHALAFTSP KKIYSADYSD ASDADLVVLT SGAPQKPGET RLDLVEKNLR
110 120 130 140 150
ITKDVVTKIV ASGFKGIFLV AANPVDILTY ATWKFSGFPK NRVVGSGTSL
160 170 180 190 200
DTARFRQALA EKVDVDARSI HAYIMGEHGD SEFAVWSHAN VAGVKLEQWF
210 220 230 240 250
QENDYLNEAE IVELFESVRD AAYSIIAKKG ATFYGVAVAL ARITKAILDD
260 270 280 290 300
EHAVLPVSVF QDGQYGVSDC YLGQPAVVGA EGVVNPIHIP LNDAEMQKME
310 320
ASGAQLKAII DEAFAKEEFA SAVKN
Length:325
Mass (Da):35,051
Last modified:January 23, 2007 - v3
Checksum:i8BFA78343AF13003
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270C → F in AAA25172 (PubMed:1400245).Curated1
Sequence conflicti270C → F in AAA99896 (PubMed:1400245).Curated1
Sequence conflicti270C → F in AAB51678 (PubMed:9023947).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95919 Genomic DNA Translation: AAA25172.1
M88490 Genomic DNA Translation: AAA25187.1
L07920 Genomic DNA Translation: AAA99896.1
U04315 Genomic DNA Translation: AAA61963.1
U02386 Genomic DNA Translation: AAA61962.1
U78632 Genomic DNA Translation: AAB51676.1
U78636 Genomic DNA Translation: AAB51680.1
U78637 Genomic DNA Translation: AAB51681.1
U78638 Genomic DNA Translation: AAB51682.1
U78634 Genomic DNA Translation: AAB51678.1
AE005176 Genomic DNA Translation: AAK05429.1
PIRiA45246
C86791
JN0449
RefSeqiNP_267487.1, NC_002662.1
WP_003131075.1, NC_002662.1

Genome annotation databases

EnsemblBacteriaiAAK05429; AAK05429; L0017
GeneIDi1114981
KEGGilla:L0017
PATRICifig|272623.7.peg.1438

Similar proteinsi

Entry informationi

Entry nameiLDH1_LACLA
AccessioniPrimary (citable) accession number: Q01462
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health