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Protein

DNA polymerase alpha-binding protein

Gene

CTF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory factor for DNA replication. It plays a role in accurately duplicating the genome in vivo.

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA binding Source: SGD

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • DNA repair Source: SGD
  • DNA replication initiation Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • establishment of sister chromatid cohesion Source: SGD
  • mitotic sister chromatid cohesion Source: SGD
  • replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-34271-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase alpha-binding protein
Alternative name(s):
Chromosome replication protein CHL15
Chromosome transmission fidelity protein 4
Protein POB1
Gene namesi
Name:CTF4
Synonyms:CHL15, POB1
Ordered Locus Names:YPR135W
ORF Names:P9659.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR135W.
SGDiS000006339. CTF4.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: SGD
  • nuclear replication fork Source: SGD
  • nucleus Source: SGD
  • replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000509482 – 927DNA polymerase alpha-binding proteinAdd BLAST926

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei377PhosphoserineCombined sources1
Modified residuei379PhosphoserineCombined sources1
Modified residuei398PhosphoserineCombined sources1
Modified residuei401PhosphothreonineCombined sources1
Modified residuei411PhosphothreonineCombined sources1
Modified residuei463PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01454.
PRIDEiQ01454.

PTM databases

iPTMnetiQ01454.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DIA2Q084963EBI-5209,EBI-31943
POL1P133825EBI-5209,EBI-6128

Protein-protein interaction databases

BioGridi36302. 370 interactors.
DIPiDIP-4640N.
IntActiQ01454. 23 interactors.
MINTiMINT-479487.

Structurei

Secondary structure

1927
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi489 – 496Combined sources8
Beta strandi498 – 507Combined sources10
Beta strandi510 – 518Combined sources9
Turni520 – 522Combined sources3
Beta strandi526 – 532Combined sources7
Beta strandi536 – 539Combined sources4
Beta strandi541 – 547Combined sources7
Turni549 – 551Combined sources3
Beta strandi553 – 560Combined sources8
Beta strandi566 – 569Combined sources4
Beta strandi578 – 583Combined sources6
Beta strandi588 – 592Combined sources5
Beta strandi596 – 600Combined sources5
Beta strandi606 – 611Combined sources6
Beta strandi615 – 621Combined sources7
Beta strandi624 – 631Combined sources8
Turni632 – 634Combined sources3
Beta strandi635 – 642Combined sources8
Beta strandi649 – 656Combined sources8
Helixi666 – 669Combined sources4
Helixi674 – 679Combined sources6
Beta strandi686 – 689Combined sources4
Beta strandi695 – 698Combined sources4
Beta strandi703 – 709Combined sources7
Turni713 – 715Combined sources3
Beta strandi717 – 723Combined sources7
Helixi724 – 731Combined sources8
Turni732 – 734Combined sources3
Beta strandi738 – 748Combined sources11
Beta strandi751 – 760Combined sources10
Beta strandi772 – 775Combined sources4
Helixi783 – 795Combined sources13
Helixi819 – 844Combined sources26
Beta strandi847 – 849Combined sources3
Helixi851 – 874Combined sources24
Turni875 – 877Combined sources3
Helixi879 – 886Combined sources8
Helixi892 – 904Combined sources13
Helixi908 – 924Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C8HX-ray2.69A/B/C471-927[»]
4C8SX-ray3.00A/B/C471-927[»]
4C93X-ray2.69A/B/C471-927[»]
4C95X-ray2.69A/B/C471-927[»]
5HOGX-ray3.09A/B/C471-927[»]
5HOIX-ray3.30A/B/C472-927[»]
ProteinModelPortaliQ01454.
SMRiQ01454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati10 – 49WD 1Add BLAST40
Repeati134 – 173WD 2Add BLAST40
Repeati227 – 266WD 3Add BLAST40
Repeati273 – 313WD 4Add BLAST41
Repeati699 – 739WD 5Add BLAST41

Sequence similaritiesi

Contains 5 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000002030.
HOGENOMiHOG000066013.
InParanoidiQ01454.
KOiK11274.
OMAiRFREYHF.
OrthoDBiEOG092C0SIN.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12341. Mcl1_mid. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET
60 70 80 90 100
LDSSKLVSSI KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC
110 120 130 140 150
CVIHSGKMAV FGGDDLELIL LELDDETHKK HAIKIDEQVS QISYNSQMNI
160 170 180 190 200
LAVSMINGKV QIFSLTSTIP NKVHELNDYI VANSYDDTHR DKILSNMMDD
210 220 230 240 250
IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK GLHFALPCAD
260 270 280 290 300
DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL
310 320 330 340 350
TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN
360 370 380 390 400
LAESVVSNIP DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI
410 420 430 440 450
TQEANAEDVF TQTHDGPSGL SEKRKYNFED EEDFIDDDDG AGYISGKKPH
460 470 480 490 500
NEHSYSRVHK THSFPISLAN TGKFRYMPFS PAGTPFGFTD RRYLTMNEVG
510 520 530 540 550
YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN EKGTLFGQSK
560 570 580 590 600
TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF
610 620 630 640 650
NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY
660 670 680 690 700
YKRECPLPMS LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS
710 720 730 740 750
DNTLLLLSKW RSPEESKWLP ILDSNMEIWK MSGGKETTDI HVWPLALAYD
760 770 780 790 800
TLNCILVKGK HIWPEFPLPL PSEMEIRMPV FVKSKLLEEN KAILNKKNEI
810 820 830 840 850
GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT LENDGEMYGN
860 870 880 890 900
ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK
910 920
ISERAELPSL VKKINNIREA RYEQQLK
Length:927
Mass (Da):104,425
Last modified:January 23, 2007 - v2
Checksum:iE2ABBB244B5F8B4D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112G → E (PubMed:1341195).Curated1
Sequence conflicti112G → E (PubMed:8316240).Curated1
Sequence conflicti588V → F (PubMed:1341195).Curated1
Sequence conflicti590V → F (PubMed:1341195).Curated1
Sequence conflicti601N → I (PubMed:1341195).Curated1
Sequence conflicti835E → K (PubMed:1341195).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94769 Genomic DNA. Translation: AAA34887.1.
S63246 Genomic DNA. Translation: AAB27308.1.
U40829 Genomic DNA. Translation: AAB68276.1.
BK006949 Genomic DNA. Translation: DAA11548.1.
PIRiA45039.
RefSeqiNP_015461.1. NM_001184232.1.

Genome annotation databases

EnsemblFungiiYPR135W; YPR135W; YPR135W.
GeneIDi856254.
KEGGisce:YPR135W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94769 Genomic DNA. Translation: AAA34887.1.
S63246 Genomic DNA. Translation: AAB27308.1.
U40829 Genomic DNA. Translation: AAB68276.1.
BK006949 Genomic DNA. Translation: DAA11548.1.
PIRiA45039.
RefSeqiNP_015461.1. NM_001184232.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C8HX-ray2.69A/B/C471-927[»]
4C8SX-ray3.00A/B/C471-927[»]
4C93X-ray2.69A/B/C471-927[»]
4C95X-ray2.69A/B/C471-927[»]
5HOGX-ray3.09A/B/C471-927[»]
5HOIX-ray3.30A/B/C472-927[»]
ProteinModelPortaliQ01454.
SMRiQ01454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36302. 370 interactors.
DIPiDIP-4640N.
IntActiQ01454. 23 interactors.
MINTiMINT-479487.

PTM databases

iPTMnetiQ01454.

Proteomic databases

MaxQBiQ01454.
PRIDEiQ01454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR135W; YPR135W; YPR135W.
GeneIDi856254.
KEGGisce:YPR135W.

Organism-specific databases

EuPathDBiFungiDB:YPR135W.
SGDiS000006339. CTF4.

Phylogenomic databases

GeneTreeiENSGT00390000002030.
HOGENOMiHOG000066013.
InParanoidiQ01454.
KOiK11274.
OMAiRFREYHF.
OrthoDBiEOG092C0SIN.

Enzyme and pathway databases

BioCyciYEAST:G3O-34271-MONOMER.

Miscellaneous databases

PROiQ01454.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12341. Mcl1_mid. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCTF4_YEAST
AccessioniPrimary (citable) accession number: Q01454
Secondary accession number(s): D6W4D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.