Reviewed, UniProtKB/Swiss-Prot Q01454 (CTF4_YEAST)

Names and origin

Protein names

Recommended name:
    DNA polymerase alpha-binding protein
Alternative name(s):
    Chromosome transmission fidelity protein 4
    Protein POB1
    Chromosome replication protein CHL15

Gene names

Name:	CTF4
Synonyms:	CHL15, POB1
Ordered Locus Names:	YPR135W
ORF Names:	P9659.7

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	927 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Accessory factor for DNA replication. It plays a role in accurately duplicating the genome in vivo.
Subcellular location	Nucleus.
Miscellaneous	Present with 3280 molecules/cell in log phase SD medium.
Sequence similarities	Contains 5 WD repeats.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Ref.1 Ref.2 Inferred from genetic interaction. Source: SGD DNA-dependent DNA replication Ref.1 Inferred from mutant phenotype. Source: SGD mitotic sister chromatid cohesion Inferred from mutant phenotype. Source: SGD replicative cell aging Inferred from mutant phenotype. Source: SGD
Cellular component	nuclear replication fork Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP40	P33203	1	EBI-5209,EBI-701
RSP5	P39940	1	EBI-5209,EBI-16219
SSM4	P40318	1	EBI-5209,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 927	926	DNA polymerase alpha-binding protein		PRO_0000050948
Regions
	Repeat	10 – 49	40	WD 1
	Repeat	134 – 173	40	WD 2
	Repeat	227 – 266	40	WD 3
	Repeat	273 – 313	41	WD 4
	Repeat	699 – 739	41	WD 5
Amino acid modifications
	Modified residue	377	1	Phosphoserine
	Modified residue	379	1	Phosphoserine
	Modified residue	401	1	Phosphothreonine
	Modified residue	411	1	Phosphothreonine
	Modified residue	463	1	Phosphoserine
Experimental info
	Sequence conflict	112	1	G → E Ref.2 Ref.4
	Sequence conflict	588	1	V → F Ref.2
	Sequence conflict	590	1	V → F Ref.2
	Sequence conflict	601	1	N → I Ref.2
	Sequence conflict	835	1	E → K Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q01454-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E2ABBB244B5F8B4D	FASTA	927	104,425
	10 20 30 40 50 60 MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET LDSSKLVSSI 70 80 90 100 110 120 KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC CVIHSGKMAV FGGDDLELIL 130 140 150 160 170 180 LELDDETHKK HAIKIDEQVS QISYNSQMNI LAVSMINGKV QIFSLTSTIP NKVHELNDYI 190 200 210 220 230 240 VANSYDDTHR DKILSNMMDD IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK 250 260 270 280 290 300 GLHFALPCAD DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL 310 320 330 340 350 360 TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN LAESVVSNIP 370 380 390 400 410 420 DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI TQEANAEDVF TQTHDGPSGL 430 440 450 460 470 480 SEKRKYNFED EEDFIDDDDG AGYISGKKPH NEHSYSRVHK THSFPISLAN TGKFRYMPFS 490 500 510 520 530 540 PAGTPFGFTD RRYLTMNEVG YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN 550 560 570 580 590 600 EKGTLFGQSK TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF 610 620 630 640 650 660 NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY YKRECPLPMS 670 680 690 700 710 720 LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS DNTLLLLSKW RSPEESKWLP 730 740 750 760 770 780 ILDSNMEIWK MSGGKETTDI HVWPLALAYD TLNCILVKGK HIWPEFPLPL PSEMEIRMPV 790 800 810 820 830 840 FVKSKLLEEN KAILNKKNEI GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT 850 860 870 880 890 900 LENDGEMYGN ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK 910 920 ISERAELPSL VKKINNIREA RYEQQLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo." Miles J., Formosa T. Mol. Cell. Biol. 12:5724-5735(1992) [PubMed: 1448101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21. Strain: 7208-12.
[2]	"CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae." Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V., Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M., Hieter P., Spencer F., Larionov V. Mol. Cell. Biol. 12:5736-5747(1992) [PubMed: 1341195] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Erratum Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V., Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M., Hieter P., Spencer F., Larionov V. Mol. Cell. Biol. 13:7202-7202(1993)
[4]	"CHL15 -- a new gene controlling the replication of chromosomes in Saccharomycetes yeast: cloning, physical mapping, sequencing, and sequence analysis." Kouprina N.Y., Kroll E.S., Koryabin M.Y., Shestopalov B.V., Bliskovsky V.V., Bannikov V.M., Gizatullin R.Z., Kirillov A.V., Kravtsov V.Y., Zakharyev V.M. Mol. Biol. (Mosk.) 27:569-588(1993) [PubMed: 8316240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377 AND SER-379, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379 AND SER-463, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379; THR-401; THR-411 AND SER-463, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94769 Genomic DNA. Translation: AAA34887.1. S63246 Genomic DNA. Translation: AAB27308.1. U40829 Genomic DNA. Translation: AAB68276.1.
PIR	A45039.
RefSeq	NP_015461.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4640N.
IntAct	Q01454.
Proteomic databases
PeptideAtlas	Q01454.
Genome annotation databases
Ensembl	YPR135W. Saccharomyces cerevisiae. [Contig view]
GeneID	856254.
GenomeReviews	Gene locus YPR135W in contig U00094_GR.
KEGG	sce:YPR135W.
NMPDR	fig|4932.3.peg.6598.
Organism-specific databases
CYGD	YPR135w.
SGD	S000006339. CTF4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q01454.
Gene expression databases
ArrayExpress	Q01454.
GermOnline	YPR135W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
SMART	SM00320. WD40. 3 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q01454.
NextBio	981537.

Entry information

Entry name

CTF4_YEAST

Accession

Primary (citable) accession number: Q01454

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)