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Protein

DNA polymerase alpha-binding protein

Gene

CTF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory factor for DNA replication. It plays a role in accurately duplicating the genome in vivo.

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA binding Source: SGD

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • DNA repair Source: SGD
  • DNA replication initiation Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • establishment of sister chromatid cohesion Source: SGD
  • mitotic sister chromatid cohesion Source: SGD
  • replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-34271-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase alpha-binding protein
Alternative name(s):
Chromosome replication protein CHL15
Chromosome transmission fidelity protein 4
Protein POB1
Gene namesi
Name:CTF4
Synonyms:CHL15, POB1
Ordered Locus Names:YPR135W
ORF Names:P9659.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR135W.
SGDiS000006339. CTF4.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: SGD
  • nuclear replication fork Source: SGD
  • nucleus Source: SGD
  • replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 927926DNA polymerase alpha-binding proteinPRO_0000050948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei377 – 3771PhosphoserineCombined sources
Modified residuei379 – 3791PhosphoserineCombined sources
Modified residuei398 – 3981PhosphoserineCombined sources
Modified residuei401 – 4011PhosphothreonineCombined sources
Modified residuei411 – 4111PhosphothreonineCombined sources
Modified residuei463 – 4631PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01454.

PTM databases

iPTMnetiQ01454.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DIA2Q084963EBI-5209,EBI-31943
POL1P133825EBI-5209,EBI-6128

Protein-protein interaction databases

BioGridi36302. 370 interactions.
DIPiDIP-4640N.
IntActiQ01454. 23 interactions.
MINTiMINT-479487.

Structurei

Secondary structure

1
927
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi489 – 4968Combined sources
Beta strandi498 – 50710Combined sources
Beta strandi510 – 5189Combined sources
Turni520 – 5223Combined sources
Beta strandi526 – 5327Combined sources
Beta strandi536 – 5394Combined sources
Beta strandi541 – 5477Combined sources
Turni549 – 5513Combined sources
Beta strandi553 – 5608Combined sources
Beta strandi566 – 5694Combined sources
Beta strandi578 – 5836Combined sources
Beta strandi588 – 5925Combined sources
Beta strandi596 – 6005Combined sources
Beta strandi606 – 6116Combined sources
Beta strandi615 – 6217Combined sources
Beta strandi624 – 6318Combined sources
Turni632 – 6343Combined sources
Beta strandi635 – 6428Combined sources
Beta strandi649 – 6568Combined sources
Helixi666 – 6694Combined sources
Helixi674 – 6796Combined sources
Beta strandi686 – 6894Combined sources
Beta strandi695 – 6984Combined sources
Beta strandi703 – 7097Combined sources
Turni713 – 7153Combined sources
Beta strandi717 – 7237Combined sources
Helixi724 – 7318Combined sources
Beta strandi738 – 74811Combined sources
Beta strandi751 – 76010Combined sources
Beta strandi772 – 7754Combined sources
Helixi783 – 79513Combined sources
Helixi819 – 84426Combined sources
Beta strandi847 – 8493Combined sources
Helixi851 – 87424Combined sources
Turni875 – 8773Combined sources
Helixi879 – 8868Combined sources
Helixi892 – 90413Combined sources
Helixi908 – 92417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C8HX-ray2.69A/B/C471-927[»]
4C8SX-ray3.00A/B/C471-927[»]
4C93X-ray2.69A/B/C471-927[»]
4C95X-ray2.69A/B/C471-927[»]
ProteinModelPortaliQ01454.
SMRiQ01454. Positions 474-927.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati10 – 4940WD 1Add
BLAST
Repeati134 – 17340WD 2Add
BLAST
Repeati227 – 26640WD 3Add
BLAST
Repeati273 – 31341WD 4Add
BLAST
Repeati699 – 73941WD 5Add
BLAST

Sequence similaritiesi

Contains 5 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000002030.
HOGENOMiHOG000066013.
InParanoidiQ01454.
KOiK11274.
OMAiRFREYHF.
OrthoDBiEOG7W41N8.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12341. Mcl1_mid. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET
60 70 80 90 100
LDSSKLVSSI KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC
110 120 130 140 150
CVIHSGKMAV FGGDDLELIL LELDDETHKK HAIKIDEQVS QISYNSQMNI
160 170 180 190 200
LAVSMINGKV QIFSLTSTIP NKVHELNDYI VANSYDDTHR DKILSNMMDD
210 220 230 240 250
IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK GLHFALPCAD
260 270 280 290 300
DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL
310 320 330 340 350
TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN
360 370 380 390 400
LAESVVSNIP DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI
410 420 430 440 450
TQEANAEDVF TQTHDGPSGL SEKRKYNFED EEDFIDDDDG AGYISGKKPH
460 470 480 490 500
NEHSYSRVHK THSFPISLAN TGKFRYMPFS PAGTPFGFTD RRYLTMNEVG
510 520 530 540 550
YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN EKGTLFGQSK
560 570 580 590 600
TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF
610 620 630 640 650
NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY
660 670 680 690 700
YKRECPLPMS LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS
710 720 730 740 750
DNTLLLLSKW RSPEESKWLP ILDSNMEIWK MSGGKETTDI HVWPLALAYD
760 770 780 790 800
TLNCILVKGK HIWPEFPLPL PSEMEIRMPV FVKSKLLEEN KAILNKKNEI
810 820 830 840 850
GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT LENDGEMYGN
860 870 880 890 900
ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK
910 920
ISERAELPSL VKKINNIREA RYEQQLK
Length:927
Mass (Da):104,425
Last modified:January 23, 2007 - v2
Checksum:iE2ABBB244B5F8B4D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121G → E (PubMed:1341195).Curated
Sequence conflicti112 – 1121G → E (PubMed:8316240).Curated
Sequence conflicti588 – 5881V → F (PubMed:1341195).Curated
Sequence conflicti590 – 5901V → F (PubMed:1341195).Curated
Sequence conflicti601 – 6011N → I (PubMed:1341195).Curated
Sequence conflicti835 – 8351E → K (PubMed:1341195).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94769 Genomic DNA. Translation: AAA34887.1.
S63246 Genomic DNA. Translation: AAB27308.1.
U40829 Genomic DNA. Translation: AAB68276.1.
BK006949 Genomic DNA. Translation: DAA11548.1.
PIRiA45039.
RefSeqiNP_015461.1. NM_001184232.1.

Genome annotation databases

EnsemblFungiiYPR135W; YPR135W; YPR135W.
GeneIDi856254.
KEGGisce:YPR135W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94769 Genomic DNA. Translation: AAA34887.1.
S63246 Genomic DNA. Translation: AAB27308.1.
U40829 Genomic DNA. Translation: AAB68276.1.
BK006949 Genomic DNA. Translation: DAA11548.1.
PIRiA45039.
RefSeqiNP_015461.1. NM_001184232.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C8HX-ray2.69A/B/C471-927[»]
4C8SX-ray3.00A/B/C471-927[»]
4C93X-ray2.69A/B/C471-927[»]
4C95X-ray2.69A/B/C471-927[»]
ProteinModelPortaliQ01454.
SMRiQ01454. Positions 474-927.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36302. 370 interactions.
DIPiDIP-4640N.
IntActiQ01454. 23 interactions.
MINTiMINT-479487.

PTM databases

iPTMnetiQ01454.

Proteomic databases

MaxQBiQ01454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR135W; YPR135W; YPR135W.
GeneIDi856254.
KEGGisce:YPR135W.

Organism-specific databases

EuPathDBiFungiDB:YPR135W.
SGDiS000006339. CTF4.

Phylogenomic databases

GeneTreeiENSGT00390000002030.
HOGENOMiHOG000066013.
InParanoidiQ01454.
KOiK11274.
OMAiRFREYHF.
OrthoDBiEOG7W41N8.

Enzyme and pathway databases

BioCyciYEAST:G3O-34271-MONOMER.

Miscellaneous databases

PROiQ01454.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12341. Mcl1_mid. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo."
    Miles J., Formosa T.
    Mol. Cell. Biol. 12:5724-5735(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
    Strain: 7208-12.
  2. "CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae."
    Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V., Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M., Hieter P., Spencer F., Larionov V.
    Mol. Cell. Biol. 12:5736-5747(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "CHL15 -- a new gene controlling the replication of chromosomes in Saccharomycetes yeast: cloning, physical mapping, sequencing, and sequence analysis."
    Kouprina N.Y., Kroll E.S., Koryabin M.Y., Shestopalov B.V., Bliskovsky V.V., Bannikov V.M., Gizatullin R.Z., Kirillov A.V., Kravtsov V.Y., Zakharyev V.M.
    Mol. Biol. (Mosk.) 27:569-588(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377 AND SER-379, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379; THR-401; THR-411 AND SER-463, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-379 AND SER-398, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCTF4_YEAST
AccessioniPrimary (citable) accession number: Q01454
Secondary accession number(s): D6W4D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.