Q01447 (ERG24_FUSSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Delta(14)-sterol reductase EC=1.3.1.70 Alternative name(s): C-14 sterol reductase Sterol C14-reductase
Organism	Fusarium solani subsp. pisi (Nectria haematococca)
Taxonomic identifier	70791 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Nectria › Nectria haematococca complex

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol.
Catalytic activity	4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	delta14-sterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Delta(14)-sterol reductase		PRO_0000207496
Regions
	Transmembrane	18 – 38	21	Helical; Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	131 – 151	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	319 – 339	21	Helical; Potential
	Transmembrane	431 – 451	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01447 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 45A828AD154B02B6	FASTA	485	54,597
	10 20 30 40 50 60 MALKSSKAVS EEQHGYEFFG PPGAFAISFL LPVLVYVFNF VCNDISGCPA PSLLSPKTLS 70 80 90 100 110 120 LDKLKQEVGW PQDGFAGLVS WEASAATAGY ILLSLILYRV LPAHEVEGTE LRSGGRLKYR 130 140 150 160 170 180 LNTLYSSSFT LAILAAGTAA QGAEFPVWTF ISDNFIQILT ANTIFSYAVA TFVYVRSFSV 190 200 210 220 230 240 KPGNKENREL AAGGHTGNML YDWFIGRELN PRVVIPLIGE VDLKEWLELR PGMMGWIIFN 250 260 270 280 290 300 CSWCAQQYRN YGYVTDSSIC ITLVQAVYVF DSWWNEPAIL TTMDITTDGF GMMLAFGDIV 310 320 330 340 350 360 WVPFVYSLQT RYLAVHPVSL GPVGLAVMLS LIGLGFYIFR SANSEKNNFR TNPNDPKVSQ 370 380 390 400 410 420 LKYIQTKKGS KLLISGWWGI ARHINYLGDW IQSWPYCLPT GLAGYQILNA GAQAEGALVM 430 440 450 460 470 480 RDGREVVQGE AKGWGMLITY FYILYFAILL IHRERRDDDK CHRKYGEDWE KYRKIVRYRI IPGIY

References

[1]	Srinivas G., Vanetten H.D., Kasbekar D.P. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MP VI.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94315 Genomic DNA. Translation: CAA63976.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG24_FUSSO

Accession

Primary (citable) accession number: Q01447

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 28, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program