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Protein

N-acetylgalactosamine kinase

Gene

GALK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts on GalNAc. Also acts as a galactokinase when galactose is present at high concentrations. May be involved in a salvage pathway for the reutilization of free GalNAc derived from the degradation of complex carbohydrates.1 Publication

Catalytic activityi

ATP + N-acetyl-alpha-D-galactosamine = ADP + N-acetyl-alpha-D-galactosamine 1-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei43Transition state stabilizerBy similarity1
Active sitei190Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 149ATP1 PublicationAdd BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • galactokinase activity Source: UniProtKB
  • N-acetylgalactosamine kinase activity Source: UniProtKB-EC

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS08161-MONOMER.
BRENDAi2.7.1.157. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylgalactosamine kinase (EC:2.7.1.157)
Alternative name(s):
GalNAc kinase
Galactokinase 2
Gene namesi
Name:GALK2
Synonyms:GK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:4119. GALK2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi2585.
OpenTargetsiENSG00000156958.
PharmGKBiPA28534.

Polymorphism and mutation databases

DMDMi399518.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001846471 – 458N-acetylgalactosamine kinaseAdd BLAST458

Proteomic databases

EPDiQ01415.
MaxQBiQ01415.
PaxDbiQ01415.
PeptideAtlasiQ01415.
PRIDEiQ01415.

PTM databases

iPTMnetiQ01415.
PhosphoSitePlusiQ01415.

Expressioni

Gene expression databases

BgeeiENSG00000156958.
CleanExiHS_GALK2.
ExpressionAtlasiQ01415. baseline and differential.
GenevisibleiQ01415. HS.

Organism-specific databases

HPAiHPA043564.
HPA048267.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi108858. 15 interactors.
MINTiMINT-5004443.
STRINGi9606.ENSP00000453129.

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 11Combined sources4
Helixi13 – 15Combined sources3
Helixi17 – 30Combined sources14
Beta strandi35 – 47Combined sources13
Helixi52 – 54Combined sources3
Beta strandi58 – 73Combined sources16
Beta strandi75 – 77Combined sources3
Beta strandi79 – 85Combined sources7
Beta strandi91 – 93Combined sources3
Beta strandi102 – 104Combined sources3
Helixi107 – 121Combined sources15
Beta strandi130 – 136Combined sources7
Beta strandi142 – 144Combined sources3
Helixi146 – 162Combined sources17
Helixi168 – 179Combined sources12
Helixi180 – 182Combined sources3
Helixi189 – 196Combined sources8
Beta strandi201 – 206Combined sources6
Turni207 – 210Combined sources4
Beta strandi211 – 215Combined sources5
Beta strandi221 – 227Combined sources7
Helixi234 – 236Combined sources3
Helixi239 – 257Combined sources19
Turni262 – 264Combined sources3
Helixi268 – 275Combined sources8
Helixi279 – 289Combined sources11
Helixi297 – 304Combined sources8
Helixi308 – 314Combined sources7
Helixi318 – 320Combined sources3
Helixi328 – 351Combined sources24
Helixi356 – 373Combined sources18
Helixi380 – 391Combined sources12
Beta strandi395 – 399Combined sources5
Beta strandi404 – 413Combined sources10
Helixi414 – 416Combined sources3
Helixi417 – 429Combined sources13
Beta strandi442 – 446Combined sources5
Beta strandi453 – 457Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A2CX-ray1.65A1-458[»]
2A2DX-ray2.20A1-458[»]
ProteinModelPortaliQ01415.
SMRiQ01415.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01415.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 52Substrate binding4
Regioni187 – 190Substrate binding4

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.Curated

Phylogenomic databases

eggNOGiKOG0631. Eukaryota.
COG0153. LUCA.
GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
HOVERGENiHBG051696.
InParanoidiQ01415.
KOiK18674.
OMAiCKSERHI.
OrthoDBiEOG091G09BP.
PhylomeDBiQ01415.
TreeFamiTF324235.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q01415-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATESPATRR VQVAEHPRLL KLKEMFNSKF GSIPKFYVRA PGRVNIIGEH
60 70 80 90 100
IDYCGYSVLP MAVEQDVLIA VEPVKTYALQ LANTNPLYPD FSTSANNIQI
110 120 130 140 150
DKTKPLWHNY FLCGLKGIQE HFGLSNLTGM NCLVDGNIPP SSGLSSSSAL
160 170 180 190 200
VCCAGLVTLT VLGRNLSKVE LAEICAKSER YIGTEGGGMD QSISFLAEEG
210 220 230 240 250
TAKLIEFSPL RATDVKLPSG AVFVIANSCV EMNKAATSHF NIRVMECRLA
260 270 280 290 300
AKLLAKYKSL QWDKVLRLEE VQAKLGISLE EMLLVTEDAL HPEPYNPEEI
310 320 330 340 350
CRCLGISLEE LRTQILSPNT QDVLIFKLYQ RAKHVYSEAA RVLQFKKICE
360 370 380 390 400
EAPENMVQLL GELMNQSHMS CRDMYECSCP ELDQLVDICR KFGAQGSRLT
410 420 430 440 450
GAGWGGCTVS MVPADKLPSF LANVHKAYYQ RSDGSLAPEK QSLFATKPGG

GALVLLEA
Length:458
Mass (Da):50,378
Last modified:July 1, 1993 - v1
Checksum:i192B774938F32947
GO
Isoform 2 (identifier: Q01415-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MATESPATRRVQVAEHP → MPVLYD

Show »
Length:447
Mass (Da):49,235
Checksum:i66D4925D605D1A4F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049123182I → V.Corresponds to variant rs35507772dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0428971 – 17MATES…VAEHP → MPVLYD in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84443 mRNA. Translation: AAA58612.1.
AF461816 mRNA. Translation: AAP97708.1.
BT006901 mRNA. Translation: AAP35547.1.
AC013452 Genomic DNA. No translation available.
AC022306 Genomic DNA. No translation available.
AC036102 Genomic DNA. No translation available.
BC005141 mRNA. Translation: AAH05141.1.
CCDSiCCDS32236.1. [Q01415-2]
CCDS42034.1. [Q01415-1]
PIRiA46366.
RefSeqiNP_001001556.1. NM_001001556.2. [Q01415-2]
NP_002035.1. NM_002044.3. [Q01415-1]
UniGeneiHs.122006.
Hs.738080.
Hs.738534.

Genome annotation databases

EnsembliENST00000327171; ENSP00000316632; ENSG00000156958. [Q01415-2]
ENST00000560031; ENSP00000453129; ENSG00000156958. [Q01415-1]
GeneIDi2585.
KEGGihsa:2585.
UCSCiuc001zxi.3. human. [Q01415-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84443 mRNA. Translation: AAA58612.1.
AF461816 mRNA. Translation: AAP97708.1.
BT006901 mRNA. Translation: AAP35547.1.
AC013452 Genomic DNA. No translation available.
AC022306 Genomic DNA. No translation available.
AC036102 Genomic DNA. No translation available.
BC005141 mRNA. Translation: AAH05141.1.
CCDSiCCDS32236.1. [Q01415-2]
CCDS42034.1. [Q01415-1]
PIRiA46366.
RefSeqiNP_001001556.1. NM_001001556.2. [Q01415-2]
NP_002035.1. NM_002044.3. [Q01415-1]
UniGeneiHs.122006.
Hs.738080.
Hs.738534.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A2CX-ray1.65A1-458[»]
2A2DX-ray2.20A1-458[»]
ProteinModelPortaliQ01415.
SMRiQ01415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108858. 15 interactors.
MINTiMINT-5004443.
STRINGi9606.ENSP00000453129.

PTM databases

iPTMnetiQ01415.
PhosphoSitePlusiQ01415.

Polymorphism and mutation databases

DMDMi399518.

Proteomic databases

EPDiQ01415.
MaxQBiQ01415.
PaxDbiQ01415.
PeptideAtlasiQ01415.
PRIDEiQ01415.

Protocols and materials databases

DNASUi2585.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327171; ENSP00000316632; ENSG00000156958. [Q01415-2]
ENST00000560031; ENSP00000453129; ENSG00000156958. [Q01415-1]
GeneIDi2585.
KEGGihsa:2585.
UCSCiuc001zxi.3. human. [Q01415-1]

Organism-specific databases

CTDi2585.
DisGeNETi2585.
GeneCardsiGALK2.
HGNCiHGNC:4119. GALK2.
HPAiHPA043564.
HPA048267.
MIMi137028. gene.
neXtProtiNX_Q01415.
OpenTargetsiENSG00000156958.
PharmGKBiPA28534.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0631. Eukaryota.
COG0153. LUCA.
GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
HOVERGENiHBG051696.
InParanoidiQ01415.
KOiK18674.
OMAiCKSERHI.
OrthoDBiEOG091G09BP.
PhylomeDBiQ01415.
TreeFamiTF324235.

Enzyme and pathway databases

BioCyciZFISH:HS08161-MONOMER.
BRENDAi2.7.1.157. 2681.

Miscellaneous databases

ChiTaRSiGALK2. human.
EvolutionaryTraceiQ01415.
GenomeRNAii2585.
PROiQ01415.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156958.
CleanExiHS_GALK2.
ExpressionAtlasiQ01415. baseline and differential.
GenevisibleiQ01415. HS.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGALK2_HUMAN
AccessioniPrimary (citable) accession number: Q01415
Secondary accession number(s): Q7Z4Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.