Q01413 (E13B_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glucan endo-1,3-beta-glucosidase B EC=3.2.1.39 Alternative name(s): (1->3)-beta-glucan endohydrolase B Short name=(1->3)-beta-glucanase B Basic beta-1,3-glucanase Beta-1,3-endoglucanase B
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole Potential.
Developmental stage	Maximum expression found during days 4 to 6 and days 4 to 14 after inoculation with an avirulent and a virulent pathogen respectively.
Induction	Upon infection by virulent and avirulent races of pathogens, for example fungal pathogen C.fulvum.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity
	Chain	26 – 340	315	Glucan endo-1,3-beta-glucosidase B		PRO_0000011852
	Propeptide	341 – 360	20	Removed in mature form Probable		PRO_0000011853
Sites
	Active site	265	1	Nucleophile By similarity
	Active site	321	1	Proton donor By similarity
Amino acid modifications
	Modified residue	26	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	350	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01413 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: EB29C1AB76347EE1	FASTA	360	39,719
	10 20 30 40 50 60 MATSQIAIIV LLGLLVATNI HITEAQIGVC YGMMGNNLPS HSEVIQLYKS RNIRRLRLYD 70 80 90 100 110 120 PNHGALNALR GSNIEVILGL PNVDVKHISS GMEHARWWVQ KNVRDFWPHV KIKYIAVGNE 130 140 150 160 170 180 ISPVTGTSNL APFQVPALVN IYKAIGEAGL GNDIKVSTSV DMTLIGNSYP PSQGSFRNDV 190 200 210 220 230 240 RWFTDPIVGF LRDTRAPLLV NIYPYFSYSG NPGQISLPYA LFTAPNVVVQ DGSRQYRNLF 250 260 270 280 290 300 DAMLDSVYAA MDRTGGGSVG IVVSESGWPS AGAFGATHEN AQTYLRNLIQ HAKEGSPRKP 310 320 330 340 350 360 GPIETYIFAM FDENNKNPEL EKHFGMFSPN KQPKYNLNFG VSERVWDITN STASSLTSEI

References

[1]

"Differential accumulation of mRNAs encoding extracellular and intracellular PR proteins in tomato induced by virulent and avirulent races of Cladosporium fulvum." van Kan J.A.L., Joosten M.H.A.J., Wagemakers C.A.M., van den Berg-Velthuis G.C.M., de Wit P.J.G.M. Plant Mol. Biol. 20:513-527(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Moneymaker. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80608 mRNA. Translation: AAA03618.1.
PIR	S26241.
RefSeq	NP_001234805.1. NM_001247876.1.
UniGene	Les.8987.
3D structure databases
ProteinModelPortal	Q01413.
SMR	Q01413. Positions 27-339.
ModBase	Search...
Protein family/group databases
Allergome	2549. Lyc e Glucanase.
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	Solyc01g059980.2.1; Solyc01g059980.2.1; Solyc01g059980.2. Solyc01g060020.2.1; Solyc01g060020.2.1; Solyc01g060020.2.
GeneID	543987.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_SOLLC

Accession

Primary (citable) accession number: Q01413

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program