Reviewed, UniProtKB/Swiss-Prot Q01412 (E13A_SOLLC)

Names and origin

Protein names	Recommended name:     Glucan endo-1,3-beta-glucosidase A     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase A       Short name=(1->3)-beta-glucanase A     Acidic beta-1,3-glucanase     Beta-1,3-endoglucanase A
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space.
Developmental stage	Maximum expression found during days 4 to 8 and days 8 to 12 after inoculation with an avirulent and a virulent pathogen respectively.
Induction	Upon infection by virulent and avirulent races of pathogens, for example fungal pathogen C.fulvum.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 336	313	Glucan endo-1,3-beta-glucosidase A		PRO_0000011851
Sites
	Active site	257	1	Nucleophile By similarity
	Active site	317	1	Proton donor By similarity
Amino acid modifications
	Modified residue	24	1	Pyrrolidone carboxylic acid Probable

Sequences

	Sequence		Length	Mass (Da)
	Q01412-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 5335FDF40DEF9488	FASTA	336	37,572
	10 20 30 40 50 60 MAFLSSLLAS LLLVGLLIQI TGAQPIGVCY GKIANNLPSD QDVIKLYNSN NIKKMRIYFP 70 80 90 100 110 120 ETNVFNALKG SNIEIILDVP NQDLEALANP SKRQGWVQDN IRNHFPDVKF KYIAVGNEVD 130 140 150 160 170 180 PGRDSGKYAR FVGPAMENIY NALSSAGLQN QIKVSTATYL GLLTNTYPPR DSIFRDEYKS 190 200 210 220 230 240 FINPIIGFLS RHNLPLLANI YPYFGHADDN VPLPYALFKQ QGLNDAGYQN LFDALVDSMY 250 260 270 280 290 300 FATEKLGGQN IEIIVSESGW PSEGHPSATL ENAMTYYTNL INHVKGGAGT PKKPGRTIET 310 320 330 YLFAMFDENR KDGKPSEQHF GLFKPDQRPK YQLKFD

References

[1]

"Differential accumulation of mRNAs encoding extracellular and intracellular PR proteins in tomato induced by virulent and avirulent races of Cladosporium fulvum." van Kan J.A.L., Joosten M.H.A.J., Wagemakers C.A.M., van den Berg-Velthuis G.C.M., de Wit P.J.G.M. Plant Mol. Biol. 20:513-527(1992) [PubMed: 1421154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 312-330. Strain: cv. Moneymaker. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80604 mRNA. Translation: AAA03617.1.
PIR	S26240.
UniGene	Les.3673
3D structure databases
SMR	Q01412. Positions 26-335.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 281054.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13A_SOLLC

Accession

Primary (citable) accession number: Q01412

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)