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Protein

Src substrate protein p85

Gene

CTTN1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the organization of the actin cytoskeleton and cell shape (By similarity). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones, and may play a role in the regulation of neuronal spine density (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (By similarity). Plays a role in endocytosis via clathrin-coated pits (By similarity).By similarity

GO - Biological processi

  1. actin cytoskeleton reorganization Source: UniProtKB
  2. actin filament polymerization Source: InterPro
  3. cell motility Source: UniProtKB
  4. focal adhesion assembly Source: UniProtKB
  5. intracellular protein transport Source: UniProtKB
  6. lamellipodium organization Source: UniProtKB
  7. neuron projection morphogenesis Source: UniProtKB
  8. positive regulation of actin filament polymerization Source: UniProtKB
  9. receptor-mediated endocytosis Source: UniProtKB
  10. regulation of axon extension Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Src substrate protein p85
Alternative name(s):
Cortactin
p80
Gene namesi
Name:CTTN1
Synonyms:EMS1, P85.25
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cell membrane 1 Publication. Endoplasmic reticulum 1 Publication. Cytoplasmcytoskeleton 1 Publication. Cell projectionlamellipodium By similarity. Cell projectionruffle By similarity. Cell projectiondendritic spine By similarity. Cell projectiondendrite By similarity. Cell projection By similarity. Cell junction By similarity. Membraneclathrin-coated pit By similarity. Cell junctionfocal adhesion By similarity. Cell projectionpodosome 1 Publication
Note: Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In normal cells, probably in association with the plasma membrane and possibly the endoplasmic reticulum (PubMed:1922035). p80/85 colocalizes with F-actin in peripheral extensions of normal cells and rosettes (podosomes) of src-transformed cells. p80/85 probably associates with components of the cytoskeleton. In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity).By similarity1 Publication

GO - Cellular componenti

  1. cell junction Source: UniProtKB
  2. coated pit Source: UniProtKB
  3. cortical cytoskeleton Source: UniProtKB
  4. dendritic spine Source: UniProtKB-SubCell
  5. endoplasmic reticulum Source: UniProtKB-SubCell
  6. lamellipodium Source: UniProtKB
  7. plasma membrane Source: UniProtKB-SubCell
  8. ruffle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 563563Src substrate protein p85PRO_0000022411Add
BLAST

Post-translational modificationi

In normal cells, appears to be phosphorylated on serine and threonine; in cells expressing activated forms of pp60-src, they become heavily phosphorylated on tyrosine in vitro. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ01406.
PRIDEiQ01406.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Dnm2P390542EBI-2530463,EBI-642337From a different organism.
KCNMA1Q8AYS82EBI-2530463,EBI-1635766

Protein-protein interaction databases

BioGridi676704. 1 interaction.
IntActiQ01406. 2 interactions.
MINTiMINT-1507003.
STRINGi9031.ENSGALP00000012463.

Structurei

3D structure databases

ProteinModelPortaliQ01406.
SMRiQ01406. Positions 498-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati89 – 12537Cortactin 1Add
BLAST
Repeati126 – 16237Cortactin 2Add
BLAST
Repeati163 – 19937Cortactin 3Add
BLAST
Repeati200 – 23637Cortactin 4Add
BLAST
Repeati237 – 27337Cortactin 5Add
BLAST
Repeati274 – 31037Cortactin 6Add
BLAST
Repeati311 – 33323Cortactin 7; truncatedAdd
BLAST
Domaini505 – 56359SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili349 – 41062Sequence AnalysisAdd
BLAST

Domaini

The SH3 motif may mediate binding to the cytoskeleton.Curated

Sequence similaritiesi

Contains 7 cortactin repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG123488.
HOGENOMiHOG000006523.
HOVERGENiHBG005994.
InParanoidiQ01406.
KOiK06106.
PhylomeDBiQ01406.

Family and domain databases

InterProiIPR015503. Cortactin.
IPR003134. Hs1_Cortactin.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10829:SF15. PTHR10829:SF15. 1 hit.
PfamiPF02218. HS1_rep. 7 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
PROSITEiPS51090. CORTACTIN. 7 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform p85 (identifier: Q01406-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVLLLVVLQ MWKATAGHSI AVSQDDGADD WETDPDFVND VSEKEQRWGA
60 70 80 90 100
KTVKGSGHQE HINIHQLREN VFQEHQTIKE KELETGPKAS HGYGGKFGVE
110 120 130 140 150
QDRMDKSAVG HEYQSKLSKH CSQVDSVKGF GGKFGVQTDR VDQSAVGFEY
160 170 180 190 200
QGKTEKHASQ KDYSSGFGGK YGVQADRVDK SAVGFDYQGK TEKHESQKDY
210 220 230 240 250
SKGFGGKYGV DKDKVDKSAV GFEYQGKTEK HESQKDYVKG FGGKFGVQTD
260 270 280 290 300
RQDKCALGWD HQEKVQLHES QKDYKSGFGG KFGVQTERQD PSAVGFDYKE
310 320 330 340 350
KLAKHESQQD YSKGFGGKYG VQKDRMDKNA ATFEDIEKPT STYQKTKPVE
360 370 380 390 400
RVANKTSSIR ANLENLAKEK EQEDRRKAEA ERAQRMAREK QEQEEARRKL
410 420 430 440 450
EEQAKAKKQT PPPSPTTQPA EPKTPSSPVY QDAVSYDAES AYKNSSTTYS
460 470 480 490 500
AEHEPESGYK TTGSDYQEAV SQREAEYEPE TVYEVAGAGD HYQAEENTYD
510 520 530 540 550
EYENELGITA IALYDYQAAG DDEISFDPDD IITNIEMIDD GWWRGVCKGR
560
YGLFPANYVE LRQ
Length:563
Mass (Da):63,329
Last modified:April 1, 1993 - v1
Checksum:i22A824A08B8D654C
GO
Isoform p80 (identifier: Q01406-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Note: No experimental confirmation available.

Show »
Length:553
Mass (Da):62,219
Checksum:i5E714902628217E8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010Missing in isoform p80. CuratedVSP_018797

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73705 mRNA. Translation: AAA49031.1.
PIRiA41530.
RefSeqiNP_990799.1. NM_205468.1. [Q01406-1]
UniGeneiGga.37455.

Genome annotation databases

GeneIDi396455.
KEGGigga:396455.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73705 mRNA. Translation: AAA49031.1.
PIRiA41530.
RefSeqiNP_990799.1. NM_205468.1. [Q01406-1]
UniGeneiGga.37455.

3D structure databases

ProteinModelPortaliQ01406.
SMRiQ01406. Positions 498-563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676704. 1 interaction.
IntActiQ01406. 2 interactions.
MINTiMINT-1507003.
STRINGi9031.ENSGALP00000012463.

Proteomic databases

PaxDbiQ01406.
PRIDEiQ01406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396455.
KEGGigga:396455.

Organism-specific databases

CTDi2017.

Phylogenomic databases

eggNOGiNOG123488.
HOGENOMiHOG000006523.
HOVERGENiHBG005994.
InParanoidiQ01406.
KOiK06106.
PhylomeDBiQ01406.

Miscellaneous databases

NextBioi20816496.
PROiQ01406.

Family and domain databases

InterProiIPR015503. Cortactin.
IPR003134. Hs1_Cortactin.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10829:SF15. PTHR10829:SF15. 1 hit.
PfamiPF02218. HS1_rep. 7 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
PROSITEiPS51090. CORTACTIN. 7 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and characterization of a novel cytoskeleton-associated pp60src substrate."
    Wu H., Reynolds A.B., Kanner S.B., Vines R.R., Parsons J.T.
    Mol. Cell. Biol. 11:5113-5124(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION.

Entry informationi

Entry nameiSRC8_CHICK
AccessioniPrimary (citable) accession number: Q01406
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 4, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.