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Q01406 (SRC8_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Src substrate protein p85
Alternative name(s):
Cortactin
p80
Gene names
Name:CTTN1
Synonyms:EMS1, P85.25
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to the organization of the actin cytoskeleton and cell structure. May be involved in the regulation of neuronal spine density By similarity. Plays a role in the regulation of cell migration.

Subcellular location

Cell membrane. Endoplasmic reticulum. Cytoplasm › cytoskeleton. Cell projection › lamellipodium By similarity. Cell projection › ruffle By similarity. Cell projection › dendritic spine By similarity. Cell projection › dendrite By similarity. Note: Associated with membrane ruffles and lamellipodia. In normal cells, probably in association with the plasma membrane and possibly the endoplasmic reticulum. p80/85 colocalizes with F-actin in peripheral extensions of normal cells and rosettes (podosomes) of src-transformed cells. p80/85 probably associates with components of the cytoskeleton. In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft By similarity.

Domain

The SH3 motif may mediate binding to the cytoskeleton.

Post-translational modification

In normal cells, appears to be phosphorylated on serine and threonine; in cells expressing activated forms of pp60-src, they become heavily phosphorylated on tyrosine in vitro. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.

Sequence similarities

Contains 7 cortactin repeats.

Contains 1 SH3 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dnm2P390542EBI-2530463,EBI-642337From a different organism.
KCNMA1Q8AYS82EBI-2530463,EBI-1635766

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform p85 (identifier: Q01406-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform p80 (identifier: Q01406-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 563563Src substrate protein p85
PRO_0000022411

Regions

Repeat89 – 12537Cortactin 1
Repeat126 – 16237Cortactin 2
Repeat163 – 19937Cortactin 3
Repeat200 – 23637Cortactin 4
Repeat237 – 27337Cortactin 5
Repeat274 – 31037Cortactin 6
Repeat311 – 33323Cortactin 7; truncated
Domain505 – 56359SH3

Natural variations

Alternative sequence1 – 1010Missing in isoform p80.
VSP_018797

Sequences

Sequence LengthMass (Da)Tools
Isoform p85 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 22A824A08B8D654C

FASTA56363,329
        10         20         30         40         50         60 
MTVLLLVVLQ MWKATAGHSI AVSQDDGADD WETDPDFVND VSEKEQRWGA KTVKGSGHQE 

        70         80         90        100        110        120 
HINIHQLREN VFQEHQTIKE KELETGPKAS HGYGGKFGVE QDRMDKSAVG HEYQSKLSKH 

       130        140        150        160        170        180 
CSQVDSVKGF GGKFGVQTDR VDQSAVGFEY QGKTEKHASQ KDYSSGFGGK YGVQADRVDK 

       190        200        210        220        230        240 
SAVGFDYQGK TEKHESQKDY SKGFGGKYGV DKDKVDKSAV GFEYQGKTEK HESQKDYVKG 

       250        260        270        280        290        300 
FGGKFGVQTD RQDKCALGWD HQEKVQLHES QKDYKSGFGG KFGVQTERQD PSAVGFDYKE 

       310        320        330        340        350        360 
KLAKHESQQD YSKGFGGKYG VQKDRMDKNA ATFEDIEKPT STYQKTKPVE RVANKTSSIR 

       370        380        390        400        410        420 
ANLENLAKEK EQEDRRKAEA ERAQRMAREK QEQEEARRKL EEQAKAKKQT PPPSPTTQPA 

       430        440        450        460        470        480 
EPKTPSSPVY QDAVSYDAES AYKNSSTTYS AEHEPESGYK TTGSDYQEAV SQREAEYEPE 

       490        500        510        520        530        540 
TVYEVAGAGD HYQAEENTYD EYENELGITA IALYDYQAAG DDEISFDPDD IITNIEMIDD 

       550        560 
GWWRGVCKGR YGLFPANYVE LRQ

« Hide

Isoform p80 [UniParc].

Checksum: 5E714902628217E8
Show »

FASTA55362,219

References

[1]"Identification and characterization of a novel cytoskeleton-associated pp60src substrate."
Wu H., Reynolds A.B., Kanner S.B., Vines R.R., Parsons J.T.
Mol. Cell. Biol. 11:5113-5124(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M73705 mRNA. Translation: AAA49031.1.
IPIIPI00573303.
IPI00760362.
PIRA41530.
RefSeqNP_990799.1. NM_205468.1.
UniGeneGga.37455.

3D structure databases

ProteinModelPortalQ01406.
SMRQ01406. Positions 498-563.
ModBaseSearch...

Protein-protein interaction databases

IntActQ01406. 2 interactions.
MINTMINT-1507003.
STRING9031.ENSGALP00000012463.

Proteomic databases

PaxDbQ01406.
PRIDEQ01406.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396455.
KEGGgga:396455.

Organism-specific databases

CTD2017.

Phylogenomic databases

eggNOGNOG123488.
HOGENOMHOG000006523.
HOVERGENHBG005994.
InParanoidQ01406.
KOK06106.
OrthoDBEOG4RNB86.

Family and domain databases

InterProIPR015503. Cortactin.
IPR003134. Hs1_Cortactin.
IPR000108. p67phox.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR10829:SF4. PTHR10829:SF4. 1 hit.
PfamPF02218. HS1_rep. 7 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00499. P67PHOX.
PR00452. SH3DOMAIN.
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SH3. 1 hit.
PROSITEPS51090. CORTACTIN. 7 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816496.

Entry information

Entry nameSRC8_CHICK
AccessionPrimary (citable) accession number: Q01406
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 29, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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