Reviewed, UniProtKB/Swiss-Prot Q01402 (NDK2_SPIOL)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase 2, chloroplastic
    EC=2.7.4.6
Alternative name(s):
    Nucleoside diphosphate kinase II
      Short name=NDP kinase II
      Short name=NDPK II
      Short name=NDK II
Cleaved into the following 2 chains:
    1- Recommended name:
            Nucleoside diphosphate kinase 2 high molecular weight
    2- Recommended name:
            Nucleoside diphosphate kinase 2 low molecular weight

Gene names

Name:

NDPK2

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 67	67	Chloroplast Potential
	Chain	68 – 233	166	Nucleoside diphosphate kinase 2 high molecular weight		PRO_0000019439
	Chain	81 – 233	153	Nucleoside diphosphate kinase 2 low molecular weight		PRO_0000019440
Sites
	Active site	199	1	Pros-phosphohistidine intermediate By similarity
	Binding site	93	1	ATP By similarity
	Binding site	141	1	ATP By similarity
	Binding site	169	1	ATP By similarity
	Binding site	175	1	ATP By similarity
	Binding site	186	1	ATP By similarity
	Binding site	196	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01402-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F4173840F71DFEBD	FASTA	233	26,107
	10 20 30 40 50 60 MEAMSGLSSP CNCISSLPHS SSTTTRHQNL LFRRNNHHQQ KLAAFHSQSH LFSTKCPLIS 70 80 90 100 110 120 HSLPRKKSFK PHIFLPHLVA SMEQVEETYI MIKPDGVQRG LVGEIISRFE KKGFKLIGLK 130 140 150 160 170 180 MYPCPKELAE EHYKDLKAKS FYQKLIDYIT SGPVVCMAWE GVGVVASSRK LIGATDPLQA 190 200 210 220 230 EPGTIRGDLA VQTGRNVVHG SDSPDNGKRE IGLWFKEGEI CQWTPAQAPW LRE

References

[1]

"Nucleotide sequence of the cDNA encoding nucleoside diphosphate kinase II from spinach leaves." Zhang J., Nomura T., Yatsunami K., Honda A., Sugimoto Y., Moriwaki T., Yamamoto J., Ohta M., Fukui T., Ichikawa A. Biochim. Biophys. Acta 1171:304-306(1993) [PubMed: 8381027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D11465 mRNA. Translation: BAA02018.1.
PIR	S28226.
3D structure databases
HSSP	HSSP built from PDB template 1NPK based on UniProtKB P22887.
SMR	Q01402. Positions 81-233.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.4.6. 286.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK2_SPIOL

Accession

Primary (citable) accession number: Q01402

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)