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Protein

1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic

Gene

SBE1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei409NucleophileBy similarity1
Active sitei464Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycogen biosynthetic process Source: InterPro
  • starch biosynthetic process Source: UniProtKB-UniPathway
  • starch metabolic process Source: Gramene
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Starch biosynthesis

Enzyme and pathway databases

BRENDAi2.4.1.18. 4460.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic (EC:2.4.1.18)
Alternative name(s):
Q-enzyme
Starch-branching enzyme
Gene namesi
Name:SBE1
Synonyms:RBE1
Ordered Locus Names:Os06g0726400, LOC_Os06g51084
ORF Names:P0017G10.8-1, P0017G10.8-2, P0548E04.28-1, P0548E04.28-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

  • amyloplast Source: Gramene
  • chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 64Chloroplast; amyloplast1 PublicationAdd BLAST64
ChainiPRO_000001114865 – 8201,4-alpha-glucan-branching enzyme, chloroplastic/amyloplasticAdd BLAST756

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65N-acetylthreonineCurated1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ01401.
PRIDEiQ01401.

Expressioni

Gene expression databases

ExpressionAtlasiQ01401. baseline and differential.
GenevisibleiQ01401. OS.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi39947.LOC_Os06g51084.1.

Structurei

Secondary structure

1820
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi72 – 74Combined sources3
Helixi78 – 81Combined sources4
Helixi83 – 88Combined sources6
Helixi89 – 110Combined sources22
Helixi113 – 116Combined sources4
Helixi117 – 121Combined sources5
Beta strandi123 – 128Combined sources6
Beta strandi131 – 137Combined sources7
Beta strandi142 – 148Combined sources7
Helixi149 – 151Combined sources3
Beta strandi166 – 173Combined sources8
Beta strandi185 – 192Combined sources8
Beta strandi199 – 201Combined sources3
Beta strandi209 – 211Combined sources3
Beta strandi214 – 217Combined sources4
Beta strandi220 – 224Combined sources5
Helixi229 – 231Combined sources3
Beta strandi247 – 253Combined sources7
Beta strandi257 – 261Combined sources5
Helixi265 – 271Combined sources7
Helixi273 – 278Combined sources6
Beta strandi283 – 288Combined sources6
Helixi295 – 297Combined sources3
Beta strandi303 – 308Combined sources6
Helixi310 – 312Combined sources3
Helixi315 – 327Combined sources13
Beta strandi331 – 336Combined sources6
Turni345 – 347Combined sources3
Helixi349 – 352Combined sources4
Helixi358 – 360Combined sources3
Beta strandi361 – 363Combined sources3
Helixi366 – 369Combined sources4
Turni372 – 375Combined sources4
Helixi384 – 401Combined sources18
Beta strandi405 – 408Combined sources4
Helixi411 – 415Combined sources5
Turni417 – 422Combined sources6
Helixi429 – 431Combined sources3
Helixi439 – 455Combined sources17
Beta strandi460 – 463Combined sources4
Turni470 – 473Combined sources4
Helixi476 – 478Combined sources3
Beta strandi484 – 487Combined sources4
Helixi491 – 501Combined sources11
Helixi504 – 506Combined sources3
Helixi509 – 517Combined sources9
Beta strandi525 – 527Combined sources3
Helixi532 – 536Combined sources5
Helixi542 – 547Combined sources6
Helixi550 – 553Combined sources4
Beta strandi556 – 559Combined sources4
Helixi563 – 583Combined sources21
Beta strandi585 – 590Combined sources6
Helixi593 – 595Combined sources3
Helixi606 – 608Combined sources3
Helixi619 – 623Combined sources5
Helixi629 – 646Combined sources18
Helixi648 – 650Combined sources3
Beta strandi654 – 660Combined sources7
Turni661 – 664Combined sources4
Beta strandi665 – 670Combined sources6
Beta strandi673 – 678Combined sources6
Beta strandi685 – 694Combined sources10
Beta strandi696 – 703Combined sources8
Helixi707 – 709Combined sources3
Helixi732 – 734Combined sources3
Beta strandi739 – 747Combined sources9
Beta strandi751 – 757Combined sources7
Turni760 – 762Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AMKX-ray1.90A66-767[»]
3AMLX-ray1.70A66-820[»]
3VU2X-ray2.23A/B66-767[»]
ProteinModelPortaliQ01401.
SMRiQ01401.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01401.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0470. Eukaryota.
COG0296. LUCA.
InParanoidiQ01401.
KOiK00700.
OMAiFVEACHL.
OrthoDBiEOG0936025M.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q01401-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLCLTSSSSS APAPLLPSLA DRPSPGIAGG GGNVRLSVVS SPRRSWPGKV
60 70 80 90 100
KTNFSVPATA RKNKTMVTVV EEVDHLPIYD LDPKLEEFKD HFNYRIKRYL
110 120 130 140 150
DQKCLIEKHE GGLEEFSKGY LKFGINTVDG ATIYREWAPA AQEAQLIGEF
160 170 180 190 200
NNWNGAKHKM EKDKFGIWSI KISHVNGKPA IPHNSKVKFR FRHGGGAWVD
210 220 230 240 250
RIPAWIRYAT FDASKFGAPY DGVHWDPPAC ERYVFKHPRP PKPDAPRIYE
260 270 280 290 300
AHVGMSGEEP EVSTYREFAD NVLPRIRANN YNTVQLMAIM EHSYYASFGY
310 320 330 340 350
HVTNFFAVSS RSGTPEDLKY LVDKAHSLGL RVLMDVVHSH ASNNVTDGLN
360 370 380 390 400
GYDVGQNTHE SYFHTGDRGY HKLWDSRLFN YANWEVLRFL LSNLRYWMDE
410 420 430 440 450
FMFDGFRFDG VTSMLYHHHG INKGFTGNYK EYFSLDTDVD AIVYMMLANH
460 470 480 490 500
LMHKLLPEAT IVAEDVSGMP VLCRPVDEGG VGFDFRLAMA IPDRWIDYLK
510 520 530 540 550
NKEDRKWSMS EIVQTLTNRR YTEKCIAYAE SHDQSIVGDK TIAFLLMDKE
560 570 580 590 600
MYTGMSDLQP ASPTINRGIA LQKMIHFITM ALGGDGYLNF MGNEFGHPEW
610 620 630 640 650
IDFPREGNNW SYDKCRRQWS LVDTDHLRYK YMNAFDQAMN ALEEEFSFLS
660 670 680 690 700
SSKQIVSDMN EKDKVIVFER GDLVFVFNFH PNKTYKGYKV GCDLPGKYRV
710 720 730 740 750
ALDSDALVFG GHGRVGHDVD HFTSPEGMPG VPETNFNNRP NSFKVLSPPR
760 770 780 790 800
TCVAYYRVDE DREELRRGGA VASGKIVTEY IDVEATSGET ISGGWKGSEK
810 820
DDCGKKGMKF VFRSSDEDCK
Length:820
Mass (Da):93,236
Last modified:March 7, 2006 - v2
Checksum:i7DE65880013A7B15
GO
Isoform 2 (identifier: Q01401-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: No experimental confirmation available.
Show »
Length:755
Mass (Da):86,635
Checksum:i56154AEC4FDB9BF9
GO

Sequence cautioni

The sequence BAA01616 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13A → P in BAA01584 (PubMed:16669000).Curated1
Sequence conflicti533D → G in AK068920 (PubMed:12869764).Curated1
Sequence conflicti738N → S in AAP68993 (Ref. 4) Curated1
Sequence conflicti789E → G in AAP68993 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0175101 – 65Missing in isoform 2. 1 PublicationAdd BLAST65

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10752 mRNA. Translation: BAA01584.1.
D10838 Genomic DNA. Translation: BAA01616.1. Sequence problems.
D11082 mRNA. Translation: BAA01855.1.
AY302112 mRNA. Translation: AAP68993.1.
AF136268 mRNA. Translation: AAD28284.1.
AP003685 Genomic DNA. Translation: BAD61712.1.
AP003685 Genomic DNA. Translation: BAD61713.1.
AP004685 Genomic DNA. Translation: BAD61804.1.
AP004685 Genomic DNA. Translation: BAD61805.1.
AP014962 Genomic DNA. Translation: BAS99598.1.
AK068920 mRNA. No translation available.
PIRiJX0243.
RefSeqiXP_015643111.1. XM_015787625.1. [Q01401-1]
XP_015643112.1. XM_015787626.1. [Q01401-2]
XP_015643113.1. XM_015787627.1. [Q01401-2]
UniGeneiOs.22169.

Genome annotation databases

EnsemblPlantsiOS06T0726400-01; OS06T0726400-01; OS06G0726400. [Q01401-1]
GeneIDi4342117.
GrameneiOS06T0726400-01; OS06T0726400-01; OS06G0726400.
KEGGiosa:4342117.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10752 mRNA. Translation: BAA01584.1.
D10838 Genomic DNA. Translation: BAA01616.1. Sequence problems.
D11082 mRNA. Translation: BAA01855.1.
AY302112 mRNA. Translation: AAP68993.1.
AF136268 mRNA. Translation: AAD28284.1.
AP003685 Genomic DNA. Translation: BAD61712.1.
AP003685 Genomic DNA. Translation: BAD61713.1.
AP004685 Genomic DNA. Translation: BAD61804.1.
AP004685 Genomic DNA. Translation: BAD61805.1.
AP014962 Genomic DNA. Translation: BAS99598.1.
AK068920 mRNA. No translation available.
PIRiJX0243.
RefSeqiXP_015643111.1. XM_015787625.1. [Q01401-1]
XP_015643112.1. XM_015787626.1. [Q01401-2]
XP_015643113.1. XM_015787627.1. [Q01401-2]
UniGeneiOs.22169.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AMKX-ray1.90A66-767[»]
3AMLX-ray1.70A66-820[»]
3VU2X-ray2.23A/B66-767[»]
ProteinModelPortaliQ01401.
SMRiQ01401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g51084.1.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiQ01401.
PRIDEiQ01401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS06T0726400-01; OS06T0726400-01; OS06G0726400. [Q01401-1]
GeneIDi4342117.
GrameneiOS06T0726400-01; OS06T0726400-01; OS06G0726400.
KEGGiosa:4342117.

Phylogenomic databases

eggNOGiKOG0470. Eukaryota.
COG0296. LUCA.
InParanoidiQ01401.
KOiK00700.
OMAiFVEACHL.
OrthoDBiEOG0936025M.

Enzyme and pathway databases

UniPathwayiUPA00152.
BRENDAi2.4.1.18. 4460.

Miscellaneous databases

EvolutionaryTraceiQ01401.

Gene expression databases

ExpressionAtlasiQ01401. baseline and differential.
GenevisibleiQ01401. OS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGB_ORYSJ
AccessioniPrimary (citable) accession number: Q01401
Secondary accession number(s): Q40664, Q5Z7Q1, Q7XZP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 7, 2006
Last modified: November 2, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Isoform 2 lacks the putative transit peptide and may be a cytosolic isoform.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.