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Q01401

- GLGB_ORYSJ

UniProt

Q01401 - GLGB_ORYSJ

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Protein

1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic

Gene

SBE1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091NucleophileBy similarity
Active sitei464 – 4641Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: Gramene
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: InterPro
  2. starch biosynthetic process Source: UniProtKB-UniPathway
  3. starch metabolic process Source: Gramene
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Starch biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic (EC:2.4.1.18)
Alternative name(s):
Q-enzyme
Starch-branching enzyme
Gene namesi
Name:SBE1
Synonyms:RBE1
Ordered Locus Names:Os06g0726400, LOC_Os06g51084
ORF Names:P0017G10.8-1, P0017G10.8-2, P0548E04.28-1, P0548E04.28-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 6

Organism-specific databases

GrameneiQ01401.

Subcellular locationi

GO - Cellular componenti

  1. amyloplast Source: Gramene
  2. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6464Chloroplast; amyloplast1 PublicationAdd
BLAST
Chaini65 – 8207561,4-alpha-glucan-branching enzyme, chloroplastic/amyloplasticPRO_0000011148Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651N-acetylthreonineCurated

Keywords - PTMi

Acetylation

Expressioni

Gene expression databases

ExpressionAtlasiQ01401. baseline.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
820
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi72 – 743
Helixi78 – 814
Helixi83 – 886
Helixi89 – 11022
Helixi113 – 1164
Helixi117 – 1215
Beta strandi123 – 1286
Beta strandi131 – 1377
Beta strandi142 – 1487
Helixi149 – 1513
Beta strandi166 – 1738
Beta strandi185 – 1928
Beta strandi199 – 2013
Beta strandi209 – 2113
Beta strandi214 – 2174
Beta strandi220 – 2245
Helixi229 – 2313
Beta strandi247 – 2537
Beta strandi257 – 2615
Helixi265 – 2717
Helixi273 – 2786
Beta strandi283 – 2886
Helixi295 – 2973
Beta strandi303 – 3086
Helixi310 – 3123
Helixi315 – 32713
Beta strandi331 – 3366
Turni345 – 3473
Helixi349 – 3524
Helixi358 – 3603
Beta strandi361 – 3633
Helixi366 – 3694
Turni372 – 3754
Helixi384 – 40118
Beta strandi405 – 4084
Helixi411 – 4155
Turni417 – 4226
Helixi429 – 4313
Helixi439 – 45517
Beta strandi460 – 4634
Turni470 – 4734
Helixi476 – 4783
Beta strandi484 – 4874
Helixi491 – 50111
Helixi504 – 5063
Helixi509 – 5179
Beta strandi525 – 5273
Helixi532 – 5365
Helixi542 – 5476
Helixi550 – 5534
Beta strandi556 – 5594
Helixi563 – 58321
Beta strandi585 – 5906
Helixi593 – 5953
Helixi606 – 6083
Helixi619 – 6235
Helixi629 – 64618
Helixi648 – 6503
Beta strandi654 – 6607
Turni661 – 6644
Beta strandi665 – 6706
Beta strandi673 – 6786
Beta strandi685 – 69410
Beta strandi696 – 7038
Helixi707 – 7093
Helixi732 – 7343
Beta strandi739 – 7479
Beta strandi751 – 7577
Turni760 – 7623

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AMKX-ray1.90A66-767[»]
3AMLX-ray1.70A66-820[»]
3VU2X-ray2.23A/B66-767[»]
ProteinModelPortaliQ01401.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01401.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0296.
InParanoidiQ01401.
KOiK00700.
OMAiTDHLRYK.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q01401-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLCLTSSSSS APAPLLPSLA DRPSPGIAGG GGNVRLSVVS SPRRSWPGKV
60 70 80 90 100
KTNFSVPATA RKNKTMVTVV EEVDHLPIYD LDPKLEEFKD HFNYRIKRYL
110 120 130 140 150
DQKCLIEKHE GGLEEFSKGY LKFGINTVDG ATIYREWAPA AQEAQLIGEF
160 170 180 190 200
NNWNGAKHKM EKDKFGIWSI KISHVNGKPA IPHNSKVKFR FRHGGGAWVD
210 220 230 240 250
RIPAWIRYAT FDASKFGAPY DGVHWDPPAC ERYVFKHPRP PKPDAPRIYE
260 270 280 290 300
AHVGMSGEEP EVSTYREFAD NVLPRIRANN YNTVQLMAIM EHSYYASFGY
310 320 330 340 350
HVTNFFAVSS RSGTPEDLKY LVDKAHSLGL RVLMDVVHSH ASNNVTDGLN
360 370 380 390 400
GYDVGQNTHE SYFHTGDRGY HKLWDSRLFN YANWEVLRFL LSNLRYWMDE
410 420 430 440 450
FMFDGFRFDG VTSMLYHHHG INKGFTGNYK EYFSLDTDVD AIVYMMLANH
460 470 480 490 500
LMHKLLPEAT IVAEDVSGMP VLCRPVDEGG VGFDFRLAMA IPDRWIDYLK
510 520 530 540 550
NKEDRKWSMS EIVQTLTNRR YTEKCIAYAE SHDQSIVGDK TIAFLLMDKE
560 570 580 590 600
MYTGMSDLQP ASPTINRGIA LQKMIHFITM ALGGDGYLNF MGNEFGHPEW
610 620 630 640 650
IDFPREGNNW SYDKCRRQWS LVDTDHLRYK YMNAFDQAMN ALEEEFSFLS
660 670 680 690 700
SSKQIVSDMN EKDKVIVFER GDLVFVFNFH PNKTYKGYKV GCDLPGKYRV
710 720 730 740 750
ALDSDALVFG GHGRVGHDVD HFTSPEGMPG VPETNFNNRP NSFKVLSPPR
760 770 780 790 800
TCVAYYRVDE DREELRRGGA VASGKIVTEY IDVEATSGET ISGGWKGSEK
810 820
DDCGKKGMKF VFRSSDEDCK
Length:820
Mass (Da):93,236
Last modified:March 7, 2006 - v2
Checksum:i7DE65880013A7B15
GO
Isoform 2 (identifier: Q01401-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: No experimental confirmation available.

Show »
Length:755
Mass (Da):86,635
Checksum:i56154AEC4FDB9BF9
GO

Sequence cautioni

The sequence BAA01616.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131A → P in BAA01584. (PubMed:16669000)Curated
Sequence conflicti533 – 5331D → G(PubMed:12869764)Curated
Sequence conflicti738 – 7381N → S in AAP68993. 1 PublicationCurated
Sequence conflicti789 – 7891E → G in AAP68993. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6565Missing in isoform 2. 1 PublicationVSP_017510Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10752 mRNA. Translation: BAA01584.1.
D10838 Genomic DNA. Translation: BAA01616.1. Sequence problems.
D11082 mRNA. Translation: BAA01855.1.
AY302112 mRNA. Translation: AAP68993.1.
AF136268 mRNA. Translation: AAD28284.1.
AP003685 Genomic DNA. Translation: BAD61712.1.
AP003685 Genomic DNA. Translation: BAD61713.1.
AP004685 Genomic DNA. Translation: BAD61804.1.
AP004685 Genomic DNA. Translation: BAD61805.1.
AK068920 mRNA. No translation available.
PIRiJX0243.
RefSeqiNP_001058629.1. NM_001065164.1. [Q01401-1]
UniGeneiOs.22169.

Genome annotation databases

EnsemblPlantsiOS06T0726400-01; OS06T0726400-01; OS06G0726400. [Q01401-1]
GeneIDi4342117.
KEGGiosa:4342117.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10752 mRNA. Translation: BAA01584.1 .
D10838 Genomic DNA. Translation: BAA01616.1 . Sequence problems.
D11082 mRNA. Translation: BAA01855.1 .
AY302112 mRNA. Translation: AAP68993.1 .
AF136268 mRNA. Translation: AAD28284.1 .
AP003685 Genomic DNA. Translation: BAD61712.1 .
AP003685 Genomic DNA. Translation: BAD61713.1 .
AP004685 Genomic DNA. Translation: BAD61804.1 .
AP004685 Genomic DNA. Translation: BAD61805.1 .
AK068920 mRNA. No translation available.
PIRi JX0243.
RefSeqi NP_001058629.1. NM_001065164.1. [Q01401-1 ]
UniGenei Os.22169.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3AMK X-ray 1.90 A 66-767 [» ]
3AML X-ray 1.70 A 66-820 [» ]
3VU2 X-ray 2.23 A/B 66-767 [» ]
ProteinModelPortali Q01401.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS06T0726400-01 ; OS06T0726400-01 ; OS06G0726400 . [Q01401-1 ]
GeneIDi 4342117.
KEGGi osa:4342117.

Organism-specific databases

Gramenei Q01401.

Phylogenomic databases

eggNOGi COG0296.
InParanoidi Q01401.
KOi K00700.
OMAi TDHLRYK.

Enzyme and pathway databases

UniPathwayi UPA00152 .

Miscellaneous databases

EvolutionaryTracei Q01401.

Gene expression databases

ExpressionAtlasi Q01401. baseline.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a cDNA encoding starch-branching enzyme, or Q-enzyme I, from rice endosperm."
    Nakamura Y., Yamanouchi H.
    Plant Physiol. 99:1265-1266(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Endosperm.
  2. "Molecular analysis of the gene encoding a rice starch branching enzyme."
    Kawasaki T., Mizuno K., Baba T., Shimada H.
    Mol. Gen. Genet. 237:10-16(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Starch branching enzymes from immature rice seeds."
    Mizuno K., Kimura K., Arai Y., Kawasaki T., Shimada H., Baba T.
    J. Biochem. 112:643-651(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 65-81.
  4. "cDNA cloning and sequence analysis of rice Sbe1 and Sbe3 genes."
    Chen X.H., Liu Q.Q., Wu H.K., Wang Z.Y., Gu M.H.
    Zhongguo Shuidao Kexue 17:109-112(2003)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Wuyunjing 7.
    Tissue: Seed.
  5. Junwang X., Zhen Z.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Nanjing 37.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  7. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiGLGB_ORYSJ
AccessioniPrimary (citable) accession number: Q01401
Secondary accession number(s): Q40664, Q5Z7Q1, Q7XZP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 7, 2006
Last modified: October 29, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Isoform 2 lacks the putative transit peptide and may be a cytosolic isoform.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3