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Q01401 (GLGB_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic

EC=2.4.1.18
Alternative name(s):
Q-enzyme
Starch-branching enzyme
Gene names
Name:SBE1
Synonyms:RBE1
Ordered Locus Names:Os06g0726400, LOC_Os06g51084
ORF Names:P0017G10.8-1, P0017G10.8-2, P0548E04.28-1, P0548E04.28-2
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length820 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway

Glycan biosynthesis; starch biosynthesis.

Subunit structure

Monomer.

Subcellular location

Plastidchloroplast. Plastidamyloplast.

Miscellaneous

Isoform 2 lacks the putative transit peptide and may be a cytosolic isoform.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence caution

The sequence BAA01616.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q01401-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q01401-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6464Chloroplast; amyloplast Ref.3
Chain65 – 8207561,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic
PRO_0000011148

Sites

Active site4091Nucleophile By similarity
Active site4641Proton donor By similarity

Amino acid modifications

Modified residue651N-acetylthreonine Probable

Natural variations

Alternative sequence1 – 6565Missing in isoform 2.
VSP_017510

Experimental info

Sequence conflict131A → P in BAA01584. Ref.1
Sequence conflict5331D → G Ref.7
Sequence conflict7381N → S in AAP68993. Ref.4
Sequence conflict7891E → G in AAP68993. Ref.4

Secondary structure

..................................................................................................................................... 820
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 7DE65880013A7B15

FASTA82093,236
        10         20         30         40         50         60 
MLCLTSSSSS APAPLLPSLA DRPSPGIAGG GGNVRLSVVS SPRRSWPGKV KTNFSVPATA 

        70         80         90        100        110        120 
RKNKTMVTVV EEVDHLPIYD LDPKLEEFKD HFNYRIKRYL DQKCLIEKHE GGLEEFSKGY 

       130        140        150        160        170        180 
LKFGINTVDG ATIYREWAPA AQEAQLIGEF NNWNGAKHKM EKDKFGIWSI KISHVNGKPA 

       190        200        210        220        230        240 
IPHNSKVKFR FRHGGGAWVD RIPAWIRYAT FDASKFGAPY DGVHWDPPAC ERYVFKHPRP 

       250        260        270        280        290        300 
PKPDAPRIYE AHVGMSGEEP EVSTYREFAD NVLPRIRANN YNTVQLMAIM EHSYYASFGY 

       310        320        330        340        350        360 
HVTNFFAVSS RSGTPEDLKY LVDKAHSLGL RVLMDVVHSH ASNNVTDGLN GYDVGQNTHE 

       370        380        390        400        410        420 
SYFHTGDRGY HKLWDSRLFN YANWEVLRFL LSNLRYWMDE FMFDGFRFDG VTSMLYHHHG 

       430        440        450        460        470        480 
INKGFTGNYK EYFSLDTDVD AIVYMMLANH LMHKLLPEAT IVAEDVSGMP VLCRPVDEGG 

       490        500        510        520        530        540 
VGFDFRLAMA IPDRWIDYLK NKEDRKWSMS EIVQTLTNRR YTEKCIAYAE SHDQSIVGDK 

       550        560        570        580        590        600 
TIAFLLMDKE MYTGMSDLQP ASPTINRGIA LQKMIHFITM ALGGDGYLNF MGNEFGHPEW 

       610        620        630        640        650        660 
IDFPREGNNW SYDKCRRQWS LVDTDHLRYK YMNAFDQAMN ALEEEFSFLS SSKQIVSDMN 

       670        680        690        700        710        720 
EKDKVIVFER GDLVFVFNFH PNKTYKGYKV GCDLPGKYRV ALDSDALVFG GHGRVGHDVD 

       730        740        750        760        770        780 
HFTSPEGMPG VPETNFNNRP NSFKVLSPPR TCVAYYRVDE DREELRRGGA VASGKIVTEY 

       790        800        810        820 
IDVEATSGET ISGGWKGSEK DDCGKKGMKF VFRSSDEDCK 

« Hide

Isoform 2 [UniParc].

Checksum: 56154AEC4FDB9BF9
Show »

FASTA75586,635

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a cDNA encoding starch-branching enzyme, or Q-enzyme I, from rice endosperm."
Nakamura Y., Yamanouchi H.
Plant Physiol. 99:1265-1266(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Endosperm.
[2]"Molecular analysis of the gene encoding a rice starch branching enzyme."
Kawasaki T., Mizuno K., Baba T., Shimada H.
Mol. Gen. Genet. 237:10-16(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Starch branching enzymes from immature rice seeds."
Mizuno K., Kimura K., Arai Y., Kawasaki T., Shimada H., Baba T.
J. Biochem. 112:643-651(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 65-81.
[4]"cDNA cloning and sequence analysis of rice Sbe1 and Sbe3 genes."
Chen X.H., Liu Q.Q., Wu H.K., Wang Z.Y., Gu M.H.
Zhongguo Shuidao Kexue 17:109-112(2003)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Wuyunjing 7.
Tissue: Seed.
[5]Junwang X., Zhen Z.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Nanjing 37.
[6]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[7]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Nipponbare.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10752 mRNA. Translation: BAA01584.1.
D10838 Genomic DNA. Translation: BAA01616.1. Sequence problems.
D11082 mRNA. Translation: BAA01855.1.
AY302112 mRNA. Translation: AAP68993.1.
AF136268 mRNA. Translation: AAD28284.1.
AP003685 Genomic DNA. Translation: BAD61712.1.
AP003685 Genomic DNA. Translation: BAD61713.1.
AP004685 Genomic DNA. Translation: BAD61804.1.
AP004685 Genomic DNA. Translation: BAD61805.1.
AK068920 mRNA. No translation available.
PIRJX0243.
RefSeqNP_001058629.1. NM_001065164.1. [Q01401-1]
UniGeneOs.22169.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3AMKX-ray1.90A66-767[»]
3AMLX-ray1.70A66-820[»]
3VU2X-ray2.23A/B66-767[»]
ProteinModelPortalQ01401.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0726400-01; OS06T0726400-01; OS06G0726400. [Q01401-1]
GeneID4342117.
KEGGosa:4342117.

Organism-specific databases

GrameneQ01401.

Phylogenomic databases

eggNOGCOG0296.
KOK00700.
OMATDHLRYK.

Enzyme and pathway databases

UniPathwayUPA00152.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ01401.

Entry information

Entry nameGLGB_ORYSJ
AccessionPrimary (citable) accession number: Q01401
Secondary accession number(s): Q40664, Q5Z7Q1, Q7XZP9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: March 7, 2006
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries