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Q01401

- GLGB_ORYSJ

UniProt

Q01401 - GLGB_ORYSJ

Protein

1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic

Gene

SBE1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei409 – 4091NucleophileBy similarity
    Active sitei464 – 4641Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: Gramene
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: InterPro
    2. starch biosynthetic process Source: UniProtKB-UniPathway
    3. starch metabolic process Source: Gramene

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Starch biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00152.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic (EC:2.4.1.18)
    Alternative name(s):
    Q-enzyme
    Starch-branching enzyme
    Gene namesi
    Name:SBE1
    Synonyms:RBE1
    Ordered Locus Names:Os06g0726400, LOC_Os06g51084
    ORF Names:P0017G10.8-1, P0017G10.8-2, P0548E04.28-1, P0548E04.28-2
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ01401.

    Subcellular locationi

    GO - Cellular componenti

    1. amyloplast Source: Gramene
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6464Chloroplast; amyloplast1 PublicationAdd
    BLAST
    Chaini65 – 8207561,4-alpha-glucan-branching enzyme, chloroplastic/amyloplasticPRO_0000011148Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei65 – 651N-acetylthreonineCurated

    Keywords - PTMi

    Acetylation

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    820
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi72 – 743
    Helixi78 – 814
    Helixi83 – 886
    Helixi89 – 11022
    Helixi113 – 1164
    Helixi117 – 1215
    Beta strandi123 – 1286
    Beta strandi131 – 1377
    Beta strandi142 – 1487
    Helixi149 – 1513
    Beta strandi166 – 1738
    Beta strandi185 – 1928
    Beta strandi199 – 2013
    Beta strandi209 – 2113
    Beta strandi214 – 2174
    Beta strandi220 – 2245
    Helixi229 – 2313
    Beta strandi247 – 2537
    Beta strandi257 – 2615
    Helixi265 – 2717
    Helixi273 – 2786
    Beta strandi283 – 2886
    Helixi295 – 2973
    Beta strandi303 – 3086
    Helixi310 – 3123
    Helixi315 – 32713
    Beta strandi331 – 3366
    Turni345 – 3473
    Helixi349 – 3524
    Helixi358 – 3603
    Beta strandi361 – 3633
    Helixi366 – 3694
    Turni372 – 3754
    Helixi384 – 40118
    Beta strandi405 – 4084
    Helixi411 – 4155
    Turni417 – 4226
    Helixi429 – 4313
    Helixi439 – 45517
    Beta strandi460 – 4634
    Turni470 – 4734
    Helixi476 – 4783
    Beta strandi484 – 4874
    Helixi491 – 50111
    Helixi504 – 5063
    Helixi509 – 5179
    Beta strandi525 – 5273
    Helixi532 – 5365
    Helixi542 – 5476
    Helixi550 – 5534
    Beta strandi556 – 5594
    Helixi563 – 58321
    Beta strandi585 – 5906
    Helixi593 – 5953
    Helixi606 – 6083
    Helixi619 – 6235
    Helixi629 – 64618
    Helixi648 – 6503
    Beta strandi654 – 6607
    Turni661 – 6644
    Beta strandi665 – 6706
    Beta strandi673 – 6786
    Beta strandi685 – 69410
    Beta strandi696 – 7038
    Helixi707 – 7093
    Helixi732 – 7343
    Beta strandi739 – 7479
    Beta strandi751 – 7577
    Turni760 – 7623

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AMKX-ray1.90A66-767[»]
    3AMLX-ray1.70A66-820[»]
    3VU2X-ray2.23A/B66-767[»]
    ProteinModelPortaliQ01401.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ01401.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0296.
    KOiK00700.
    OMAiTDHLRYK.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q01401-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLCLTSSSSS APAPLLPSLA DRPSPGIAGG GGNVRLSVVS SPRRSWPGKV    50
    KTNFSVPATA RKNKTMVTVV EEVDHLPIYD LDPKLEEFKD HFNYRIKRYL 100
    DQKCLIEKHE GGLEEFSKGY LKFGINTVDG ATIYREWAPA AQEAQLIGEF 150
    NNWNGAKHKM EKDKFGIWSI KISHVNGKPA IPHNSKVKFR FRHGGGAWVD 200
    RIPAWIRYAT FDASKFGAPY DGVHWDPPAC ERYVFKHPRP PKPDAPRIYE 250
    AHVGMSGEEP EVSTYREFAD NVLPRIRANN YNTVQLMAIM EHSYYASFGY 300
    HVTNFFAVSS RSGTPEDLKY LVDKAHSLGL RVLMDVVHSH ASNNVTDGLN 350
    GYDVGQNTHE SYFHTGDRGY HKLWDSRLFN YANWEVLRFL LSNLRYWMDE 400
    FMFDGFRFDG VTSMLYHHHG INKGFTGNYK EYFSLDTDVD AIVYMMLANH 450
    LMHKLLPEAT IVAEDVSGMP VLCRPVDEGG VGFDFRLAMA IPDRWIDYLK 500
    NKEDRKWSMS EIVQTLTNRR YTEKCIAYAE SHDQSIVGDK TIAFLLMDKE 550
    MYTGMSDLQP ASPTINRGIA LQKMIHFITM ALGGDGYLNF MGNEFGHPEW 600
    IDFPREGNNW SYDKCRRQWS LVDTDHLRYK YMNAFDQAMN ALEEEFSFLS 650
    SSKQIVSDMN EKDKVIVFER GDLVFVFNFH PNKTYKGYKV GCDLPGKYRV 700
    ALDSDALVFG GHGRVGHDVD HFTSPEGMPG VPETNFNNRP NSFKVLSPPR 750
    TCVAYYRVDE DREELRRGGA VASGKIVTEY IDVEATSGET ISGGWKGSEK 800
    DDCGKKGMKF VFRSSDEDCK 820
    Length:820
    Mass (Da):93,236
    Last modified:March 7, 2006 - v2
    Checksum:i7DE65880013A7B15
    GO
    Isoform 2 (identifier: Q01401-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-65: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:755
    Mass (Da):86,635
    Checksum:i56154AEC4FDB9BF9
    GO

    Sequence cautioni

    The sequence BAA01616.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131A → P in BAA01584. (PubMed:16669000)Curated
    Sequence conflicti533 – 5331D → G(PubMed:12869764)Curated
    Sequence conflicti738 – 7381N → S in AAP68993. 1 PublicationCurated
    Sequence conflicti789 – 7891E → G in AAP68993. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6565Missing in isoform 2. 1 PublicationVSP_017510Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10752 mRNA. Translation: BAA01584.1.
    D10838 Genomic DNA. Translation: BAA01616.1. Sequence problems.
    D11082 mRNA. Translation: BAA01855.1.
    AY302112 mRNA. Translation: AAP68993.1.
    AF136268 mRNA. Translation: AAD28284.1.
    AP003685 Genomic DNA. Translation: BAD61712.1.
    AP003685 Genomic DNA. Translation: BAD61713.1.
    AP004685 Genomic DNA. Translation: BAD61804.1.
    AP004685 Genomic DNA. Translation: BAD61805.1.
    AK068920 mRNA. No translation available.
    PIRiJX0243.
    RefSeqiNP_001058629.1. NM_001065164.1. [Q01401-1]
    UniGeneiOs.22169.

    Genome annotation databases

    EnsemblPlantsiOS06T0726400-01; OS06T0726400-01; OS06G0726400. [Q01401-1]
    GeneIDi4342117.
    KEGGiosa:4342117.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10752 mRNA. Translation: BAA01584.1 .
    D10838 Genomic DNA. Translation: BAA01616.1 . Sequence problems.
    D11082 mRNA. Translation: BAA01855.1 .
    AY302112 mRNA. Translation: AAP68993.1 .
    AF136268 mRNA. Translation: AAD28284.1 .
    AP003685 Genomic DNA. Translation: BAD61712.1 .
    AP003685 Genomic DNA. Translation: BAD61713.1 .
    AP004685 Genomic DNA. Translation: BAD61804.1 .
    AP004685 Genomic DNA. Translation: BAD61805.1 .
    AK068920 mRNA. No translation available.
    PIRi JX0243.
    RefSeqi NP_001058629.1. NM_001065164.1. [Q01401-1 ]
    UniGenei Os.22169.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3AMK X-ray 1.90 A 66-767 [» ]
    3AML X-ray 1.70 A 66-820 [» ]
    3VU2 X-ray 2.23 A/B 66-767 [» ]
    ProteinModelPortali Q01401.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0726400-01 ; OS06T0726400-01 ; OS06G0726400 . [Q01401-1 ]
    GeneIDi 4342117.
    KEGGi osa:4342117.

    Organism-specific databases

    Gramenei Q01401.

    Phylogenomic databases

    eggNOGi COG0296.
    KOi K00700.
    OMAi TDHLRYK.

    Enzyme and pathway databases

    UniPathwayi UPA00152 .

    Miscellaneous databases

    EvolutionaryTracei Q01401.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a cDNA encoding starch-branching enzyme, or Q-enzyme I, from rice endosperm."
      Nakamura Y., Yamanouchi H.
      Plant Physiol. 99:1265-1266(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Endosperm.
    2. "Molecular analysis of the gene encoding a rice starch branching enzyme."
      Kawasaki T., Mizuno K., Baba T., Shimada H.
      Mol. Gen. Genet. 237:10-16(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Starch branching enzymes from immature rice seeds."
      Mizuno K., Kimura K., Arai Y., Kawasaki T., Shimada H., Baba T.
      J. Biochem. 112:643-651(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 65-81.
    4. "cDNA cloning and sequence analysis of rice Sbe1 and Sbe3 genes."
      Chen X.H., Liu Q.Q., Wu H.K., Wang Z.Y., Gu M.H.
      Zhongguo Shuidao Kexue 17:109-112(2003)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. Wuyunjing 7.
      Tissue: Seed.
    5. Junwang X., Zhen Z.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. Nanjing 37.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    7. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiGLGB_ORYSJ
    AccessioniPrimary (citable) accession number: Q01401
    Secondary accession number(s): Q40664, Q5Z7Q1, Q7XZP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Isoform 2 lacks the putative transit peptide and may be a cytosolic isoform.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3