Reviewed, UniProtKB/Swiss-Prot Q01401 (GLGB_ORYSJ)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic
    EC=2.4.1.18
Alternative name(s):
    Starch-branching enzyme
    Q-enzyme

Gene names

Name:	SBE1
Synonyms:	RBE1
Ordered Locus Names:	Os06g0726400, LOC_Os06g51084
ORF Names:	P0017G10.8-1, P0017G10.8-2, P0548E04.28-1, P0548E04.28-2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.
Pathway	Glycan biosynthesis; starch biosynthesis.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast. Plastid › amyloplast.
Miscellaneous	Isoform 2 lacks the putative transit peptide and may be a cytosolic isoform.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Sequence caution	The sequence BAA01616.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Starch biosynthesis
Cellular component	Amyloplast Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	starch biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	amyloplast Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1,4-alpha-glucan branching enzyme activity Ref.2 Inferred from expression pattern. Source: Gramene cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q01401-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q01401-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 64	64	Chloroplast; amyloplast Ref.3
	Chain	65 – 820	756	1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic		PRO_0000011148
Sites
	Active site	300	1	By similarity
	Active site	335	1	By similarity
	Active site	340	1	By similarity
	Active site	407	1	By similarity
	Active site	409	1	By similarity
	Active site	464	1	By similarity
	Active site	533	1	By similarity
Amino acid modifications
	Modified residue	65	1	N-acetylthreonine Probable
Natural variations
	Alternative sequence	1 – 65	65	Missing in isoform 2.		VSP_017510
Experimental info
	Sequence conflict	13	1	A → P in BAA01584. Ref.1
	Sequence conflict	533	1	D → G Ref.7
	Sequence conflict	738	1	N → S in AAP68993. Ref.4
	Sequence conflict	789	1	E → G in AAP68993. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 7DE65880013A7B15	FASTA	820	93,236
	10 20 30 40 50 60 MLCLTSSSSS APAPLLPSLA DRPSPGIAGG GGNVRLSVVS SPRRSWPGKV KTNFSVPATA 70 80 90 100 110 120 RKNKTMVTVV EEVDHLPIYD LDPKLEEFKD HFNYRIKRYL DQKCLIEKHE GGLEEFSKGY 130 140 150 160 170 180 LKFGINTVDG ATIYREWAPA AQEAQLIGEF NNWNGAKHKM EKDKFGIWSI KISHVNGKPA 190 200 210 220 230 240 IPHNSKVKFR FRHGGGAWVD RIPAWIRYAT FDASKFGAPY DGVHWDPPAC ERYVFKHPRP 250 260 270 280 290 300 PKPDAPRIYE AHVGMSGEEP EVSTYREFAD NVLPRIRANN YNTVQLMAIM EHSYYASFGY 310 320 330 340 350 360 HVTNFFAVSS RSGTPEDLKY LVDKAHSLGL RVLMDVVHSH ASNNVTDGLN GYDVGQNTHE 370 380 390 400 410 420 SYFHTGDRGY HKLWDSRLFN YANWEVLRFL LSNLRYWMDE FMFDGFRFDG VTSMLYHHHG 430 440 450 460 470 480 INKGFTGNYK EYFSLDTDVD AIVYMMLANH LMHKLLPEAT IVAEDVSGMP VLCRPVDEGG 490 500 510 520 530 540 VGFDFRLAMA IPDRWIDYLK NKEDRKWSMS EIVQTLTNRR YTEKCIAYAE SHDQSIVGDK 550 560 570 580 590 600 TIAFLLMDKE MYTGMSDLQP ASPTINRGIA LQKMIHFITM ALGGDGYLNF MGNEFGHPEW 610 620 630 640 650 660 IDFPREGNNW SYDKCRRQWS LVDTDHLRYK YMNAFDQAMN ALEEEFSFLS SSKQIVSDMN 670 680 690 700 710 720 EKDKVIVFER GDLVFVFNFH PNKTYKGYKV GCDLPGKYRV ALDSDALVFG GHGRVGHDVD 730 740 750 760 770 780 HFTSPEGMPG VPETNFNNRP NSFKVLSPPR TCVAYYRVDE DREELRRGGA VASGKIVTEY 790 800 810 820 IDVEATSGET ISGGWKGSEK DDCGKKGMKF VFRSSDEDCK
	Isoform 2. Checksum: 56154AEC4FDB9BF9	FASTA	755	86,635
	10 20 30 40 50 60 MVTVVEEVDH LPIYDLDPKL EEFKDHFNYR IKRYLDQKCL IEKHEGGLEE FSKGYLKFGI 70 80 90 100 110 120 NTVDGATIYR EWAPAAQEAQ LIGEFNNWNG AKHKMEKDKF GIWSIKISHV NGKPAIPHNS 130 140 150 160 170 180 KVKFRFRHGG GAWVDRIPAW IRYATFDASK FGAPYDGVHW DPPACERYVF KHPRPPKPDA 190 200 210 220 230 240 PRIYEAHVGM SGEEPEVSTY REFADNVLPR IRANNYNTVQ LMAIMEHSYY ASFGYHVTNF 250 260 270 280 290 300 FAVSSRSGTP EDLKYLVDKA HSLGLRVLMD VVHSHASNNV TDGLNGYDVG QNTHESYFHT 310 320 330 340 350 360 GDRGYHKLWD SRLFNYANWE VLRFLLSNLR YWMDEFMFDG FRFDGVTSML YHHHGINKGF 370 380 390 400 410 420 TGNYKEYFSL DTDVDAIVYM MLANHLMHKL LPEATIVAED VSGMPVLCRP VDEGGVGFDF 430 440 450 460 470 480 RLAMAIPDRW IDYLKNKEDR KWSMSEIVQT LTNRRYTEKC IAYAESHDQS IVGDKTIAFL 490 500 510 520 530 540 LMDKEMYTGM SDLQPASPTI NRGIALQKMI HFITMALGGD GYLNFMGNEF GHPEWIDFPR 550 560 570 580 590 600 EGNNWSYDKC RRQWSLVDTD HLRYKYMNAF DQAMNALEEE FSFLSSSKQI VSDMNEKDKV 610 620 630 640 650 660 IVFERGDLVF VFNFHPNKTY KGYKVGCDLP GKYRVALDSD ALVFGGHGRV GHDVDHFTSP 670 680 690 700 710 720 EGMPGVPETN FNNRPNSFKV LSPPRTCVAY YRVDEDREEL RRGGAVASGK IVTEYIDVEA 730 740 750 TSGETISGGW KGSEKDDCGK KGMKFVFRSS DEDCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cDNA encoding starch-branching enzyme, or Q-enzyme I, from rice endosperm." Nakamura Y., Yamanouchi H. Plant Physiol. 99:1265-1266(1992) [PubMed: 16669000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Endosperm.
[2]	"Molecular analysis of the gene encoding a rice starch branching enzyme." Kawasaki T., Mizuno K., Baba T., Shimada H. Mol. Gen. Genet. 237:10-16(1993) [PubMed: 8455548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Starch branching enzymes from immature rice seeds." Mizuno K., Kimura K., Arai Y., Kawasaki T., Shimada H., Baba T. J. Biochem. 112:643-651(1992) [PubMed: 1478924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 65-81.
[4]	"cDNA cloning and sequence analysis of rice Sbe1 and Sbe3 genes." Chen X.H., Liu Q.Q., Wu H.K., Wang Z.Y., Gu M.H. Zhongguo Shuidao Kexue 17:109-112(2003) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Wuyunjing 7. Tissue: Seed.
[5]	Junwang X., Zhen Z. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Nanjing 37.
[6]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[7]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10752 mRNA. Translation: BAA01584.1. D10838 Genomic DNA. Translation: BAA01616.1. Sequence problems. D11082 mRNA. Translation: BAA01855.1. AY302112 mRNA. Translation: AAP68993.1. AF136268 mRNA. Translation: AAD28284.1. AP003685 Genomic DNA. Translation: BAD61712.1. AP003685 Genomic DNA. Translation: BAD61713.1. AP004685 Genomic DNA. Translation: BAD61804.1. AP004685 Genomic DNA. Translation: BAD61805.1. AK068920 mRNA. No translation available.
PIR	JX0243.
RefSeq	NP_001058629.1.
UniGene	Os.22169
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	4342117.
KEGG	osa:4342117.
Organism-specific databases
Gramene	Q01401.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLGB_ORYSJ

Accession

Primary (citable) accession number: Q01401
Secondary accession number(s): Q40664, Q5Z7Q1, Q7XZP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)