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Protein

Serine/threonine-protein kinase BCK1/SLK1/SSP31

Gene

BCK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase involved in a signal transduction pathway that plays a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act on this kinase. BCK1 probably phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1204ATPPROSITE-ProRule annotation1
Active sitei1303Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1181 – 1189ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase kinase activity Source: SGD
  • protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: SGD
  • establishment of cell polarity Source: SGD
  • intracellular signal transduction Source: SGD
  • pexophagy Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
  • response to acidic pH Source: SGD
  • response to nutrient Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31550-MONOMER.
ReactomeiR-SCE-2559580. Oxidative Stress Induced Senescence.
R-SCE-2871796. FCERI mediated MAPK activation.
R-SCE-389357. CD28 dependent PI3K/Akt signaling.
R-SCE-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5676590. NIK-->noncanonical NF-kB signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BCK1/SLK1/SSP31 (EC:2.7.11.1)
Gene namesi
Name:BCK1
Synonyms:LAS3, SLK1, SSP31
Ordered Locus Names:YJL095W
ORF Names:J0906
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL095W.
SGDiS000003631. BCK1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1119T → P in BCK1-19; Dominant active. 1 Publication1
Mutagenesisi1120I → K in BCK1-11; Dominant active. 1 Publication1
Mutagenesisi1120I → T in BCK1-16; Dominant active. 1 Publication1
Mutagenesisi1146G → V in BCK1-10; Dominant active. 1 Publication1
Mutagenesisi1174A → P in BCK1-20; Dominant active. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856621 – 1478Serine/threonine-protein kinase BCK1/SLK1/SSP31Add BLAST1478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei407PhosphothreonineCombined sources1
Modified residuei411PhosphoserineCombined sources1
Modified residuei491PhosphoserineCombined sources1
Modified residuei747PhosphoserineCombined sources1
Modified residuei816PhosphoserineCombined sources1
Modified residuei1058PhosphoserineCombined sources1
Modified residuei1061PhosphoserineCombined sources1
Modified residuei1134Phosphoserine; by PKCSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01389.
PRIDEiQ01389.

PTM databases

iPTMnetiQ01389.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006844EBI-3470,EBI-4192
MYO3P360066EBI-3470,EBI-11670
MYO5Q044396EBI-3470,EBI-11687

Protein-protein interaction databases

BioGridi33662. 564 interactors.
DIPiDIP-2223N.
IntActiQ01389. 56 interactors.
MINTiMINT-604289.

Structurei

3D structure databases

ProteinModelPortaliQ01389.
SMRiQ01389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1175 – 1440Protein kinasePROSITE-ProRule annotationAdd BLAST266

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00800000124036.
HOGENOMiHOG000095188.
InParanoidiQ01389.
KOiK11229.
OMAiLAYDNFA.
OrthoDBiEOG092C030N.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFLRKIAGT AHTHSRSDSN SSVKFGHQPT SSVASTKSSS KSPRATSRKS
60 70 80 90 100
IYDDIRSQFP NLTPNSTSSQ FYESTPVIEQ SFNWTTDDHI SAGTLENPTS
110 120 130 140 150
FTNSSYKNDN GPSSLSDSRK SSGGNSVNSL SFDKLILSWD PTDPDEWTMH
160 170 180 190 200
RVTSWFKFHD FPESWILFFK KHQLFGHRFI KLLAYDNFAV YEKYLPQTKT
210 220 230 240 250
ASYTRFQQLL KKTMTKNVTN SHIRQKSASK LKSSRSSSES IKSKLKNSKS
260 270 280 290 300
QEDISNSRST SESALSPTKS GPSKTDEKNF LHSTSTHQKT KSASSLYRRS
310 320 330 340 350
FISLRGSSSS NASSAKSPSN IKLSIPARPH SIIESNSTLT KSASPPASPS
360 370 380 390 400
YPSIFRRHHK SSSSESSLLN SLFGSGIGEE APTKPNPQGH SLSSENLAKG
410 420 430 440 450
KSKHYETNVS SPLKQSSLPT SDDKGNLWNK FKRKSQIGVP SPNTVAYVTS
460 470 480 490 500
QETPSLKSNS STATLTVQTA DVNIPSPSSS PPPIPKTANR SLEVISTEDT
510 520 530 540 550
PKISSTTASF KETYPDCINP DKTVPVPVNN QKYSVKNFLL DQKFYPLKKT
560 570 580 590 600
GLNDSENKYI LVTKDNVSFV PLNLKSVAKL SSFKESALTK LGINHKNVTF
610 620 630 640 650
HMTDFDCDIG AAIPDDTLEF LKKSLFLNTS GKIYIKDQMK LQQKPKPAPL
660 670 680 690 700
TSENNVPLKS VKSKSSMRSG TSSLIASTDD VSIVTSSSDI TSFDEHASGS
710 720 730 740 750
GRRYPQTPSY YYDRVSNTNP TEELNYWNIK EVLSHEENAP KMVFKTSPKL
760 770 780 790 800
ELNLPDKGSK LNIPTPITEN ESKSSFQVLR KDEGTEIDFN HRRESPYTKP
810 820 830 840 850
ELAPKREAPK PPANTSPQRT LSTSKQNKPI RLVRASTKIS RSKRSKPLPP
860 870 880 890 900
QLLSSPIEAS SSSPDSLTSS YTPASTHVLI PQPYKGANDV MRRLKTDQDS
910 920 930 940 950
TSTSPSLKMK QKVNRSNSTV STSNSIFYSP SPLLKRGNSK RVVSSTSAAD
960 970 980 990 1000
IFEENDITFA DAPPMFDSDD SDDDSSSSDD IIWSKKKTAP ETNNENKKDE
1010 1020 1030 1040 1050
KSDNSSTHSD EIFYDSQTQD KMERKMTFRP SPEVVYQNLE KFFPRANLDK
1060 1070 1080 1090 1100
PITEGIASPT SPKSLDSLLS PKNVASSRTE PSTPSRPVPP DSSYEFIQDG
1110 1120 1130 1140 1150
LNGKNKPLNQ AKTPKRTKTI RTIAHEASLA RKNSVKLKRQ NTKMWGTRMV
1160 1170 1180 1190 1200
EVTENHMVSI NKAKNSKGEY KEFAWMKGEM IGKGSFGAVY LCLNVTTGEM
1210 1220 1230 1240 1250
MAVKQVEVPK YSSQNEAILS TVEALRSEVS TLKDLDHLNI VQYLGFENKN
1260 1270 1280 1290 1300
NIYSLFLEYV AGGSVGSLIR MYGRFDEPLI KHLTTQVLKG LAYLHSKGIL
1310 1320 1330 1340 1350
HRDMKADNLL LDQDGICKIS DFGISRKSKD IYSNSDMTMR GTVFWMAPEM
1360 1370 1380 1390 1400
VDTKQGYSAK VDIWSLGCIV LEMFAGKRPW SNLEVVAAMF KIGKSKSAPP
1410 1420 1430 1440 1450
IPEDTLPLIS QIGRNFLDAC FEINPEKRPT ANELLSHPFS EVNETFNFKS
1460 1470
TRLAKFIKSN DKLNSSKLRI TSQENKTE
Length:1,478
Mass (Da):164,195
Last modified:October 1, 1993 - v1
Checksum:iD586C3A497A5BB33
GO

Sequence cautioni

The sequence AAA21179 differs from that shown. Reason: Frameshift at position 1086.Curated
The sequence AAA21179 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59F → I in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti79E → V in BAA01226 (PubMed:1840547).Curated1
Sequence conflicti264A → P in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti279N → I in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti703 – 714RYPQT…YYYDR → STPKPRVITMTE in CAA42788 (PubMed:1729597).CuratedAdd BLAST12
Sequence conflicti795S → A in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti802L → V in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti808A → S in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti903T → N in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti919T → N in CAA42788 (PubMed:1729597).Curated1
Sequence conflicti960A → R in AAA21179 (Ref. 7) Curated1
Sequence conflicti962A → R in AAA21179 (Ref. 7) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84389 Genomic DNA. No translation available.
D10389 Genomic DNA. Translation: BAA01226.1.
X60227 Genomic DNA. Translation: CAA42788.1.
X77923 Genomic DNA. Translation: CAA54896.1.
Z49370 Genomic DNA. Translation: CAA89389.1.
Z49369 Genomic DNA. Translation: CAA89388.1.
M88604 Genomic DNA. Translation: AAA21179.1. Sequence problems.
BK006943 Genomic DNA. Translation: DAA08705.1.
PIRiS20117.
RefSeqiNP_012440.1. NM_001181528.1.

Genome annotation databases

EnsemblFungiiYJL095W; YJL095W; YJL095W.
GeneIDi853350.
KEGGisce:YJL095W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84389 Genomic DNA. No translation available.
D10389 Genomic DNA. Translation: BAA01226.1.
X60227 Genomic DNA. Translation: CAA42788.1.
X77923 Genomic DNA. Translation: CAA54896.1.
Z49370 Genomic DNA. Translation: CAA89389.1.
Z49369 Genomic DNA. Translation: CAA89388.1.
M88604 Genomic DNA. Translation: AAA21179.1. Sequence problems.
BK006943 Genomic DNA. Translation: DAA08705.1.
PIRiS20117.
RefSeqiNP_012440.1. NM_001181528.1.

3D structure databases

ProteinModelPortaliQ01389.
SMRiQ01389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33662. 564 interactors.
DIPiDIP-2223N.
IntActiQ01389. 56 interactors.
MINTiMINT-604289.

PTM databases

iPTMnetiQ01389.

Proteomic databases

MaxQBiQ01389.
PRIDEiQ01389.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL095W; YJL095W; YJL095W.
GeneIDi853350.
KEGGisce:YJL095W.

Organism-specific databases

EuPathDBiFungiDB:YJL095W.
SGDiS000003631. BCK1.

Phylogenomic databases

GeneTreeiENSGT00800000124036.
HOGENOMiHOG000095188.
InParanoidiQ01389.
KOiK11229.
OMAiLAYDNFA.
OrthoDBiEOG092C030N.

Enzyme and pathway databases

BioCyciYEAST:G3O-31550-MONOMER.
ReactomeiR-SCE-2559580. Oxidative Stress Induced Senescence.
R-SCE-2871796. FCERI mediated MAPK activation.
R-SCE-389357. CD28 dependent PI3K/Akt signaling.
R-SCE-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5676590. NIK-->noncanonical NF-kB signaling.

Miscellaneous databases

PROiQ01389.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCK1_YEAST
AccessioniPrimary (citable) accession number: Q01389
Secondary accession number(s): D6VW89, P32894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 112 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.