SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q01389

- BCK1_YEAST

UniProt

Q01389 - BCK1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Serine/threonine-protein kinase BCK1/SLK1/SSP31
Gene
BCK1, LAS3, SLK1, SSP31, YJL095W, J0906
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act on this kinase. BCK1 probably phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1204 – 12041ATP By similarity
Active sitei1303 – 13031Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1181 – 11899ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase kinase kinase activity Source: SGD
  3. protein binding Source: IntAct
  4. protein tyrosine kinase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. MAPK cascade Source: GOC
  2. activation of MAPKK activity Source: GOC
  3. endoplasmic reticulum unfolded protein response Source: SGD
  4. establishment of cell polarity Source: SGD
  5. intracellular signal transduction Source: SGD
  6. peroxisome degradation Source: SGD
  7. protein phosphorylation Source: SGD
  8. regulation of fungal-type cell wall organization Source: SGD
  9. response to acid Source: SGD
  10. response to nutrient Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31550-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BCK1/SLK1/SSP31 (EC:2.7.11.1)
Gene namesi
Name:BCK1
Synonyms:LAS3, SLK1, SSP31
Ordered Locus Names:YJL095W
ORF Names:J0906
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL095w.
SGDiS000003631. BCK1.

Subcellular locationi

Cytoplasm Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1119 – 11191T → P in BCK1-19; Dominant active. 1 Publication
Mutagenesisi1120 – 11201I → K in BCK1-11; Dominant active. 1 Publication
Mutagenesisi1120 – 11201I → T in BCK1-16; Dominant active. 1 Publication
Mutagenesisi1146 – 11461G → V in BCK1-10; Dominant active. 1 Publication
Mutagenesisi1174 – 11741A → P in BCK1-20; Dominant active. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14781478Serine/threonine-protein kinase BCK1/SLK1/SSP31
PRO_0000085662Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei407 – 4071Phosphothreonine1 Publication
Modified residuei411 – 4111Phosphoserine1 Publication
Modified residuei491 – 4911Phosphoserine2 Publications
Modified residuei747 – 7471Phosphoserine1 Publication
Modified residuei816 – 8161Phosphoserine1 Publication
Modified residuei1058 – 10581Phosphoserine2 Publications
Modified residuei1061 – 10611Phosphoserine1 Publication
Modified residuei1134 – 11341Phosphoserine; by PKC Reviewed prediction

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01389.
PaxDbiQ01389.
PRIDEiQ01389.

Expressioni

Gene expression databases

GenevestigatoriQ01389.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006844EBI-3470,EBI-4192
MYO3P360066EBI-3470,EBI-11670
MYO5Q044396EBI-3470,EBI-11687

Protein-protein interaction databases

BioGridi33662. 564 interactions.
DIPiDIP-2223N.
IntActiQ01389. 56 interactions.
MINTiMINT-604289.

Structurei

3D structure databases

ProteinModelPortaliQ01389.
SMRiQ01389. Positions 1139-1463.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1175 – 1440266Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117575.
HOGENOMiHOG000095188.
KOiK11229.
OMAiNTKMWGT.
OrthoDBiEOG71K6B8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q01389-1 [UniParc]FASTAAdd to Basket

« Hide

MPFLRKIAGT AHTHSRSDSN SSVKFGHQPT SSVASTKSSS KSPRATSRKS     50
IYDDIRSQFP NLTPNSTSSQ FYESTPVIEQ SFNWTTDDHI SAGTLENPTS 100
FTNSSYKNDN GPSSLSDSRK SSGGNSVNSL SFDKLILSWD PTDPDEWTMH 150
RVTSWFKFHD FPESWILFFK KHQLFGHRFI KLLAYDNFAV YEKYLPQTKT 200
ASYTRFQQLL KKTMTKNVTN SHIRQKSASK LKSSRSSSES IKSKLKNSKS 250
QEDISNSRST SESALSPTKS GPSKTDEKNF LHSTSTHQKT KSASSLYRRS 300
FISLRGSSSS NASSAKSPSN IKLSIPARPH SIIESNSTLT KSASPPASPS 350
YPSIFRRHHK SSSSESSLLN SLFGSGIGEE APTKPNPQGH SLSSENLAKG 400
KSKHYETNVS SPLKQSSLPT SDDKGNLWNK FKRKSQIGVP SPNTVAYVTS 450
QETPSLKSNS STATLTVQTA DVNIPSPSSS PPPIPKTANR SLEVISTEDT 500
PKISSTTASF KETYPDCINP DKTVPVPVNN QKYSVKNFLL DQKFYPLKKT 550
GLNDSENKYI LVTKDNVSFV PLNLKSVAKL SSFKESALTK LGINHKNVTF 600
HMTDFDCDIG AAIPDDTLEF LKKSLFLNTS GKIYIKDQMK LQQKPKPAPL 650
TSENNVPLKS VKSKSSMRSG TSSLIASTDD VSIVTSSSDI TSFDEHASGS 700
GRRYPQTPSY YYDRVSNTNP TEELNYWNIK EVLSHEENAP KMVFKTSPKL 750
ELNLPDKGSK LNIPTPITEN ESKSSFQVLR KDEGTEIDFN HRRESPYTKP 800
ELAPKREAPK PPANTSPQRT LSTSKQNKPI RLVRASTKIS RSKRSKPLPP 850
QLLSSPIEAS SSSPDSLTSS YTPASTHVLI PQPYKGANDV MRRLKTDQDS 900
TSTSPSLKMK QKVNRSNSTV STSNSIFYSP SPLLKRGNSK RVVSSTSAAD 950
IFEENDITFA DAPPMFDSDD SDDDSSSSDD IIWSKKKTAP ETNNENKKDE 1000
KSDNSSTHSD EIFYDSQTQD KMERKMTFRP SPEVVYQNLE KFFPRANLDK 1050
PITEGIASPT SPKSLDSLLS PKNVASSRTE PSTPSRPVPP DSSYEFIQDG 1100
LNGKNKPLNQ AKTPKRTKTI RTIAHEASLA RKNSVKLKRQ NTKMWGTRMV 1150
EVTENHMVSI NKAKNSKGEY KEFAWMKGEM IGKGSFGAVY LCLNVTTGEM 1200
MAVKQVEVPK YSSQNEAILS TVEALRSEVS TLKDLDHLNI VQYLGFENKN 1250
NIYSLFLEYV AGGSVGSLIR MYGRFDEPLI KHLTTQVLKG LAYLHSKGIL 1300
HRDMKADNLL LDQDGICKIS DFGISRKSKD IYSNSDMTMR GTVFWMAPEM 1350
VDTKQGYSAK VDIWSLGCIV LEMFAGKRPW SNLEVVAAMF KIGKSKSAPP 1400
IPEDTLPLIS QIGRNFLDAC FEINPEKRPT ANELLSHPFS EVNETFNFKS 1450
TRLAKFIKSN DKLNSSKLRI TSQENKTE 1478
Length:1,478
Mass (Da):164,195
Last modified:October 1, 1993 - v1
Checksum:iD586C3A497A5BB33
GO

Sequence cautioni

The sequence AAA21179.1 differs from that shown. Reason: Frameshift at position 1086.
The sequence AAA21179.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591F → I in CAA42788. 1 Publication
Sequence conflicti79 – 791E → V in BAA01226. 1 Publication
Sequence conflicti264 – 2641A → P in CAA42788. 1 Publication
Sequence conflicti279 – 2791N → I in CAA42788. 1 Publication
Sequence conflicti703 – 71412RYPQT…YYYDR → STPKPRVITMTE in CAA42788. 1 Publication
Add
BLAST
Sequence conflicti795 – 7951S → A in CAA42788. 1 Publication
Sequence conflicti802 – 8021L → V in CAA42788. 1 Publication
Sequence conflicti808 – 8081A → S in CAA42788. 1 Publication
Sequence conflicti903 – 9031T → N in CAA42788. 1 Publication
Sequence conflicti919 – 9191T → N in CAA42788. 1 Publication
Sequence conflicti960 – 9601A → R in AAA21179. 1 Publication
Sequence conflicti962 – 9621A → R in AAA21179. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84389 Genomic DNA. No translation available.
D10389 Genomic DNA. Translation: BAA01226.1.
X60227 Genomic DNA. Translation: CAA42788.1.
X77923 Genomic DNA. Translation: CAA54896.1.
Z49370 Genomic DNA. Translation: CAA89389.1.
Z49369 Genomic DNA. Translation: CAA89388.1.
M88604 Genomic DNA. Translation: AAA21179.1. Sequence problems.
BK006943 Genomic DNA. Translation: DAA08705.1.
PIRiS20117.
RefSeqiNP_012440.1. NM_001181528.1.

Genome annotation databases

EnsemblFungiiYJL095W; YJL095W; YJL095W.
GeneIDi853350.
KEGGisce:YJL095W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84389 Genomic DNA. No translation available.
D10389 Genomic DNA. Translation: BAA01226.1 .
X60227 Genomic DNA. Translation: CAA42788.1 .
X77923 Genomic DNA. Translation: CAA54896.1 .
Z49370 Genomic DNA. Translation: CAA89389.1 .
Z49369 Genomic DNA. Translation: CAA89388.1 .
M88604 Genomic DNA. Translation: AAA21179.1 . Sequence problems.
BK006943 Genomic DNA. Translation: DAA08705.1 .
PIRi S20117.
RefSeqi NP_012440.1. NM_001181528.1.

3D structure databases

ProteinModelPortali Q01389.
SMRi Q01389. Positions 1139-1463.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33662. 564 interactions.
DIPi DIP-2223N.
IntActi Q01389. 56 interactions.
MINTi MINT-604289.

Proteomic databases

MaxQBi Q01389.
PaxDbi Q01389.
PRIDEi Q01389.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL095W ; YJL095W ; YJL095W .
GeneIDi 853350.
KEGGi sce:YJL095W.

Organism-specific databases

CYGDi YJL095w.
SGDi S000003631. BCK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117575.
HOGENOMi HOG000095188.
KOi K11229.
OMAi NTKMWGT.
OrthoDBi EOG71K6B8.

Enzyme and pathway databases

BioCyci YEAST:G3O-31550-MONOMER.

Miscellaneous databases

NextBioi 973753.

Gene expression databases

Genevestigatori Q01389.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast cell morphogenesis and cell growth."
    Costigan C., Gehrung S., Snyder M.
    Mol. Cell. Biol. 12:1162-1178(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A new protein kinase, SSP31, modulating the SMP3 gene-product involved in plasmid maintenance in Saccharomyces cerevisiae."
    Irie K., Araki H., Oshima Y.
    Gene 108:139-144(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homolog."
    Lee K.S., Levin D.E.
    Mol. Cell. Biol. 12:172-182(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF THR-1119; ILE-1120; GLY-1146 AND ALA-1174.
    Strain: ATCC 204278 / EG123 / SM1058.
  4. "Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene."
    Miosga T., Boles E., Schaaff-Gerstenschlaeger I., Schmitt S., Zimmermann F.K.
    Yeast 10:1481-1488(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cusick M.E.
    Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 459-1173.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-747 AND SER-1058, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-407; SER-411; SER-491; SER-816; SER-1058 AND SER-1061, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBCK1_YEAST
AccessioniPrimary (citable) accession number: Q01389
Secondary accession number(s): D6VW89, P32894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 112 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi