Q01371 (WC1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
White collar 1 protein
Short name=WC1

Gene names

Name:	wc-1
ORF Names:	NCU02356

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	1167 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as a transcription factor involved in light regulation. Binds and affects blue light regulation of the AL-3 gene. WC1 and WC2 proteins interact via homologous PAS domains, bind to promoters of light regulated genes such as FRQ, and activate transcription.
Subunit structure	Heterodimer of WC1 and WC2 Potential.
Subcellular location	Nucleus.
Induction	By blue light.
Domain	The glutamine-rich domain might function in activating gene expression.
Post-translational modification	FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark By similarity.
Sequence similarities	Contains 1 GATA-type zinc finger. Contains 2 PAC (PAS-associated C-terminal) domains. Contains 3 PAS (PER-ARNT-SIM) domains.
Sequence caution	The sequence EAA30541.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Sensory transduction Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Chromophore DNA-binding FMN Flavoprotein Metal-binding Zinc
Molecular function	Activator Photoreceptor protein Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1167	1167	White collar 1 protein		PRO_0000083489
Regions
	Domain	381 – 452	72	PAS 1
	Domain	469 – 508	40	PAC 1
	Domain	574 – 644	71	PAS 2
	Domain	650 – 691	42	PAC 2
	Domain	693 – 763	71	PAS 3
	Zinc finger	934 – 959	26	GATA-type
	Compositional bias	16 – 61	46	Gln-rich
	Compositional bias	21 – 57	37	Poly-Gln
	Compositional bias	329 – 333	5	Poly-Pro
Amino acid modifications
	Modified residue	428	1	S-4a-FMN cysteine By similarity
Experimental info
	Sequence conflict	1167	1	V → Q in EAA30541. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q01371 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 6489D04DAB50EE38	FASTA	1,167	127,454
	10 20 30 40 50 60 MNNNYYGSPL SPEELQHQMH QHQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ HQHQQQQKTN 70 80 90 100 110 120 QHRNAGMMNT PPTTNQGNST IHASDVTMSG GSDSLDEIIQ QNLDEMHRRR SVPQPYGGQT 130 140 150 160 170 180 RRLSMFDYAN PNDGFSDYQL DNMSGNYGDM TGGMGMSGHS SPYAGQNIMA MSDHSGGYSH 190 200 210 220 230 240 MSPNVMGNMM TYPNLNMYHS PPIENPYSSA GLDTIRTDFS MDMNMDSGSV SAASVHPTPG 250 260 270 280 290 300 LNKQDDEMMT MEQGFGGGDD ANASHQAQQN MGGLTPAMTP AMTPAMTPGV SNFAQGMATP 310 320 330 340 350 360 VSQDAASTPA TTFQSPSLSA TTQTIRIGPP PPPSVTNAPT PAPFTSTPSG GGASQTKSIY 370 380 390 400 410 420 SKSGFDMLRA LWYVASRKDP KLKLGAVDMS CAFVVCDVTL NDCPIIYVSD NFQNLTGYSR 430 440 450 460 470 480 HEIVGRNCRF LQAPDGNVEA GTKREFVENN AVYTLKKTIA EGQEIQQSLI NYRKGGKPFL 490 500 510 520 530 540 NLLTMIPIPW DTEEIRYFIG FQIDLVECPD AIIGQEGNGP MQVNYTHSDI GQYIWTPPTQ 550 560 570 580 590 600 KQLEPADGQT LGVDDVSTLL QQCNSKGVAS DWHKQSWDKM LLENADDVVH VLSLKGLFLY 610 620 630 640 650 660 LSPACKKVLE YDASDLVGTS LSSICHPSDI VPVTRELKEA QQHTPVNIVF RIRRKNSGYT 670 680 690 700 710 720 WFESHGTLFN EQGKGRKCII LVGRKRPVFA LHRKDLELNG GIGDSEIWTK VSTSGMFLFV 730 740 750 760 770 780 SSNVRSLLDL LPENLQGTSM QDLMRKESRA EFGRTIEKAR KGKIASCKHE VQNKRGQVLQ 790 800 810 820 830 840 AYTTFYPGDG GEGQRPTFLL AQTKLLKASS RTLAPATVTV KNMSPGGVPL SPMKGIQTDS 850 860 870 880 890 900 DSNTLMGGMS KSGSSDSTGA MVSARSSAGP GQDAALDADN IFDELKTTRC TSWQYELRQM 910 920 930 940 950 960 EKVNRMLAEE LAQLLSNKKK RKRRKGGGNM VRDCANCHTR NTPEWRRGPS GNRDLCNSCG 970 980 990 1000 1010 1020 LRWAKQTGRV SPRTSSRGGN GDSMSKKSNS PSHSSPLHRE VGNDSPSTTT ATKNSPSLRG 1030 1040 1050 1060 1070 1080 SSTTAPGTIT TDSGPAVASS ASGTGSTTIA TSANSAASTV NALGPPATGP SGGSPAQHLP 1090 1100 1110 1120 1130 1140 PHLQGTHLNA QAMQRVHQHK QHQQHQQQHQ QQHQQQHQQQ HQQLQQHQFN PPQSQPLLEG 1150 1160 GSGFRGSGME MTSIREEMGE HQQGLSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"White collar-1, a central regulator of blue light responses in Neurospora, is a zinc finger protein." Ballario P., Vittorioso P., Magrelli A., Talora C., Cabibbo A., Macino G. EMBO J. 15:1650-1657(1996) [PubMed: 8612589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	Ballario P. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94300 Genomic DNA. Translation: CAA63964.2. AABX02000010 Genomic DNA. Translation: EAA30541.2. Sequence problems.
RefSeq	XP_959777.2. XM_954684.2.
UniGene	Ncr.25268.
3D structure databases
ProteinModelPortal	Q01371.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1155N.
IntAct	Q01371. 3 interactions.
MINT	MINT-7033864.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000003067; EFNCRP00000003067; EFNCRG00000003064.
GeneID	3875924.
KEGG	ncr:NCU02356.
Phylogenomic databases
eggNOG	fuNOG07259.
GeneTree	EFGT00050000000349.
OrthoDB	EOG47WRWS.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR001610. PAC. IPR000014. PAS. IPR013655. PAS_fold_3. IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit. G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
Pfam	PF00320. GATA. 1 hit. PF08447. PAS_3. 1 hit. [Graphical view]
SMART	SM00086. PAC. 2 hits. SM00091. PAS. 3 hits. SM00401. ZnF_GATA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00229. Sensory_box. 2 hits.
PROSITE	PS00344. GATA_ZN_FINGER_1. 1 hit. PS50114. GATA_ZN_FINGER_2. 1 hit. PS50113. PAC. False negative. PS50112. PAS. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WC1_NEUCR

Accession

Primary (citable) accession number: Q01371
Secondary accession number(s): Q7RVA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 11, 2001
Last modified:	December 14, 2011
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program