Q01365 (POL2_BAYMG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Genome polyprotein 2

Cleaved into the following 2 chains:

1. Helper component proteinase
   Short name=HC-pro
   EC=3.4.22.45
2. 70 kDa protein

Gene names

Name:

RNA2

Organism

Barley yellow mosaic virus (isolate Germany) (BaYMV) [Complete proteome]

Taxonomic identifier

31728 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Potyviridae › Bymovirus

Virus host

Hordeum vulgare (Barley) [TaxID: 4513]

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Post-translational modification	The viral RNA2 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least two individual proteins. The HC-pro cleaves its C-terminus autocatalytically Potential.
Sequence similarities	Belongs to the bymoviruses polyprotein 2 family. Contains 1 peptidase C6 domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	viral capsid Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Helper component proteinase Potential		PRO_0000040554
	Chain	256 – 890	635	70 kDa protein		PRO_0000040555
Sites
	Active site	143	1	For helper component proteinase activity By similarity
	Active site	215	1	For helper component proteinase activity By similarity
	Site	255 – 256	2	Cleavage; by HC-PRO protease Potential

Sequences

	Sequence		Length	Mass (Da)
	Q01365 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 753AB79EABAD3640	FASTA	890	98,204
	10 20 30 40 50 60 MSTSSSRLFF DCGSLDWPNK SLFGDPTTRD VMDEHISSTW NAVIRRHMLA PNADAETILG 70 80 90 100 110 120 RDGLPSAQFD AYGAMLPSFI QALNAPTTRL RISAPLSTAE SILCADASHA PWLYMANSVC 130 140 150 160 170 180 AYEATHLQPV QTFIAFNFAH GYCYLSLFIP LSFRITPENA RSFSRFLEQL PDILGAYPTL 190 200 210 220 230 240 ASLYKTMLFA VRLFPEVLQA PIPIIAKRPG VLQFHVSDAR GLPPSWFPMK CGSVASFIAL 250 260 270 280 290 300 ITNNLNSDLL NGIVGSNGDG EHYTNWNSGH NHWIVNRFIT VKDLHSSLKS ALEVDLDTEG 310 320 330 340 350 360 GRNAVLDLLL DLGVTNLVRR EKRFPAYFQG AESVYLLLSC ERVGNELVAV QDALQEPLAN 370 380 390 400 410 420 YTGKDLRALI INLGGLPSRH PEICYTRNIF ENDNHLVWNF EFYRIASITK NAQIDRDVLS 430 440 450 460 470 480 SSMANLFSDF VSESSNGEYR VKEPRPVTQY RVEHDEPVAS GAPSAWWQVL VGITTAILGA 490 500 510 520 530 540 IIFFLWRCFL RAKRVKFQAK DSFPWFTTSG DDDLPPPPGD SPSRPPGRSP DRVLPRTVVR 550 560 570 580 590 600 DLSFNDDDDL HSVDLNEAGS RFGEVVSLIA RGNLRELAGA IPESLSNLTL LQTSASGSGF 610 620 630 640 650 660 YTMVALYLAT LGDAITAFHE HNDASPATIQ SLRTLELQLE ARGLRFNEAG TPANLIQRGV 670 680 690 700 710 720 KSSVGRALVR LTQSALLATG ENFRTRMAAT LERIAAERLN TLTAYDQRVI EMTTELLAAI 730 740 750 760 770 780 KTALEVERSE LTPHLANAEA LLQVYNNLFS TDYASASLLA LRREMILRSA EGRVGEQPTS 790 800 810 820 830 840 ASDAANEELV QRSMTKLDKE IELFQAQIDS QRRAVTITEA SNLRENILQP INTVANIAMA 850 860 870 880 890 GAFLRGGARH RMPGIPDVAA PMSNPFRAFS GRGHSLTTTR GAGLFRRPRV

References

[1]	"The nucleotide sequence of RNA 2 of barley yellow mosaic virus." Davidson A.D., Proels M., Schell J., Steinbiss H.H. J. Gen. Virol. 72:989-993(1991) [PubMed: 2016598] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D01099 Genomic RNA. Translation: BAA00884.1.
PIR	JQ1947.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C06.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001456. Peptidase_C6. IPR001337. TMV-like_coat. [Graphical view]
Pfam	PF00851. Peptidase_C6. 1 hit. PF00721. TMV_coat. 1 hit. [Graphical view]
SUPFAM	SSF47195. TMV_coat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

POL2_BAYMG

Accession

Primary (citable) accession number: Q01365

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program