Reviewed, UniProtKB/Swiss-Prot Q01344 (IL5RA_HUMAN)

Names and origin

Protein names

Recommended name:
    Interleukin-5 receptor subunit alpha
      Short name=IL-5R-alpha
Alternative name(s):
    CDw125
    CD_antigen=CD125

Gene names

Name:	IL5RA
Synonyms:	IL5R

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is the receptor for interleukin-5. The alpha chain binds to IL5.
Subunit structure	Heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors. Interacts with SDCBP.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on eosinophils and basophils.
Domain	The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Sequence similarities	Belongs to the type I cytokine receptor family. Type 5 subfamily.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane
Molecular function	Receptor
PTM	Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell proliferation Ref.2 Traceable author statement. Source: ProtInc signal transduction Ref.2 Traceable author statement. Source: ProtInc
Cellular component	extracellular space Ref.3 Traceable author statement. Source: ProtInc integral to membrane Ref.3 Traceable author statement. Source: ProtInc
Molecular function	interleukin-5 receptor activity Ref.3 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GRB2	P62993	1	EBI-1759442,EBI-401755

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q01344-1)

Also known as: Membrane-bound;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q01344-2)

Also known as: Soluble-S1;

The sequence of this isoform differs from the canonical sequence as follows:
     333-335: NDE → FSR
     336-420: Missing.

Isoform 3 (identifier: Q01344-3)

Also known as: Soluble-S2;

The sequence of this isoform differs from the canonical sequence as follows:
     333-333: N → K
     334-420: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 420	400	Interleukin-5 receptor subunit alpha		PRO_0000010893
Regions
	Topological domain	21 – 342	322	Extracellular Potential
	Transmembrane	343 – 362	20	Potential
	Topological domain	363 – 420	58	Cytoplasmic Potential
	Motif	322 – 326	5	WSXWS motif
	Motif	371 – 379	9	Box 1 motif
Amino acid modifications
	Glycosylation	35	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	244	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	333 – 335	3	NDE → FSR in isoform 2.		VSP_001678
	Alternative sequence	333	1	N → K in isoform 3.		VSP_001680
	Alternative sequence	334 – 420	87	Missing in isoform 3.		VSP_001681
	Alternative sequence	336 – 420	85	Missing in isoform 2.		VSP_001679
	Natural variant	129	1	I → V: dbSNP rs2290610. Ref.3		VAR_020654
	Natural variant	262	1	V → A: dbSNP rs17879690. Ref.3		VAR_020655
Experimental info
	Sequence conflict	212	1	A → S in AAA36110. Ref.1
	Sequence conflict	212	1	A → S in AAA59151. Ref.2
	Sequence conflict	212	1	A → S in AAA59152. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Membrane-bound) [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 4E0A5F2838B9C4FE	FASTA	420	47,685
	10 20 30 40 50 60 MIIVAHVLLI LLGATEILQA DLLPDEKISL LPPVNFTIKV TGLAQVLLQW KPNPDQEQRN 70 80 90 100 110 120 VNLEYQVKIN APKEDDYETR ITESKCVTIL HKGFSASVRT ILQNDHSLLA SSWASAELHA 130 140 150 160 170 180 PPGSPGTSIV NLTCTTNTTE DNYSRLRSYQ VSLHCTWLVG TDAPEDTQYF LYYRYGSWTE 190 200 210 220 230 240 ECQEYSKDTL GRNIACWFPR TFILSKGRDW LAVLVNGSSK HSAIRPFDQL FALHAIDQIN 250 260 270 280 290 300 PPLNVTAEIE GTRLSIQWEK PVSAFPIHCF DYEVKIHNTR NGYLQIEKLM TNAFISIIDD 310 320 330 340 350 360 LSKYDVQVRA AVSSMCREAG LWSEWSQPIY VGNDEHKPLR EWFVIVIMAT ICFILLILSL 370 380 390 400 410 420 ICKICHLWIK LFPPIPAPKS NIKDLFVTTN YEKAGSSETE IEVICYIEKP GVETLEDSVF
	Isoform 2 (Soluble-S1). Checksum: BAED076239845E16	FASTA	335	37,984
	10 20 30 40 50 60 MIIVAHVLLI LLGATEILQA DLLPDEKISL LPPVNFTIKV TGLAQVLLQW KPNPDQEQRN 70 80 90 100 110 120 VNLEYQVKIN APKEDDYETR ITESKCVTIL HKGFSASVRT ILQNDHSLLA SSWASAELHA 130 140 150 160 170 180 PPGSPGTSIV NLTCTTNTTE DNYSRLRSYQ VSLHCTWLVG TDAPEDTQYF LYYRYGSWTE 190 200 210 220 230 240 ECQEYSKDTL GRNIACWFPR TFILSKGRDW LAVLVNGSSK HSAIRPFDQL FALHAIDQIN 250 260 270 280 290 300 PPLNVTAEIE GTRLSIQWEK PVSAFPIHCF DYEVKIHNTR NGYLQIEKLM TNAFISIIDD 310 320 330 LSKYDVQVRA AVSSMCREAG LWSEWSQPIY VGFSR
	Isoform 3 (Soluble-S2). Checksum: 8D9239845E16985B	FASTA	333	37,722
	10 20 30 40 50 60 MIIVAHVLLI LLGATEILQA DLLPDEKISL LPPVNFTIKV TGLAQVLLQW KPNPDQEQRN 70 80 90 100 110 120 VNLEYQVKIN APKEDDYETR ITESKCVTIL HKGFSASVRT ILQNDHSLLA SSWASAELHA 130 140 150 160 170 180 PPGSPGTSIV NLTCTTNTTE DNYSRLRSYQ VSLHCTWLVG TDAPEDTQYF LYYRYGSWTE 190 200 210 220 230 240 ECQEYSKDTL GRNIACWFPR TFILSKGRDW LAVLVNGSSK HSAIRPFDQL FALHAIDQIN 250 260 270 280 290 300 PPLNVTAEIE GTRLSIQWEK PVSAFPIHCF DYEVKIHNTR NGYLQIEKLM TNAFISIIDD 310 320 330 LSKYDVQVRA AVSSMCREAG LWSEWSQPIY VGK

References

[1]	"A human high affinity interleukin-5 receptor (IL5R) is composed of an IL5-specific alpha chain and a beta chain shared with the receptor for GM-CSF." Tavernier J., Devos R., Cornelis S., Tuypens T., van der Heyden J., Fiers W., Plaetinck G. Cell 66:1175-1184(1991) [PubMed: 1833065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Molecular cloning and expression of the human interleukin 5 receptor." Murata Y., Takaki S., Migita M., Kikuchi Y., Tominaga A., Takatsu K. J. Exp. Med. 175:341-351(1992) [PubMed: 1732409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), VARIANT VAL-129. Tissue: Peripheral blood.
[3]	"Molecular basis of the membrane-anchored and two soluble isoforms of the human interleukin 5 receptor alpha subunit." Tavernier J., Tuypens T., Plaetinck G., Verhee A., Fiers W., Devos R. Proc. Natl. Acad. Sci. U.S.A. 89:7041-7045(1992) [PubMed: 1495999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
[4]	SeattleSNPs variation discovery resource Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-129 AND ALA-262.
[5]	"Molecular roots of degenerate specificity in syntenin's PDZ2 domain: reassessment of the PDZ recognition paradigm." Kang B.S., Cooper D.R., Devedjiev Y., Derewenda U., Derewenda Z.S. Structure 11:845-853(2003) [PubMed: 12842047] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 413-420 IN COMPLEX WITH SDCBP.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases

EMBLGenBankDDBJ

M75914 mRNA. Translation: AAA36110.1.
X61176 mRNA. Translation: CAA43483.1.
X62156 mRNA. Translation: CAA44081.1.
M96651 mRNA. Translation: AAA59151.1.
M96652 mRNA. Translation: AAA59152.1.
AY642135 Genomic DNA. Translation: AAT45457.1.

IPI

IPI00221146.
IPI00306687.
IPI00395487.

PIR

A40267.

RefSeq

NP_000555.2.
NP_783851.1.
NP_783852.1.
NP_783853.1.
NP_783854.1.

UniGene

Hs.68876

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OBX	X-ray	1.35	B	413-420	[»]
1OBZ	X-ray	1.70	P	413-420	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:3510N.

IntAct

Q01344. 1 interaction.

PTM databases

PhosphoSite

Q01344.

Proteomic databases

PRIDE

Q01344.

Genome annotation databases

Ensembl

ENSG00000091181. Homo sapiens. [Contig view]

GeneID

3568.

KEGG

hsa:3568.

Organism-specific databases

GeneCards

GC03M003086.

H-InvDB

HIX0003005.

HGNC

HGNC:6017. IL5RA.

HPA

HPA013196.

MIM

147851. gene.

PharmGKB

PA29834.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q01344.

HOVERGEN

Q01344.

OMA

Q01344. SLHCTWL.

Gene expression databases

ArrayExpress

Q01344.

Bgee

Q01344.

CleanEx

HS_IL5RA.

GermOnline

ENSG00000091181. Homo sapiens.

Family and domain databases

InterPro

IPR008957. Fibronectin_typ-III-like_fold.
IPR003532. Short_hematopoietin_rcpt_2_CS.
[Graphical view]

Gene3D

G3DSA:2.60.40.30. FN_III-like. 1 hit.

Pfam

PF10556. Haemat_rec_S_F2. 1 hit.
[Graphical view]

PROSITE

PS01356. HEMATOPO_REC_S_F2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

13936.

SOURCE

Search...

Entry information

Entry name

IL5RA_HUMAN

Accession

Primary (citable) accession number: Q01344
Secondary accession number(s): Q14633, Q15469, Q6ISX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)