Q01339 (APOH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-2-glycoprotein 1

Alternative name(s):
APC inhibitor
Activated protein C-binding protein
Apolipoprotein H
Short name=Apo-H
Beta-2-glycoprotein I
Short name=B2GPI
Short name=Beta(2)GPI

Gene names

Name:	Apoh
Synonyms:	B2gp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Subcellular location	Secreted.
Tissue specificity	Expressed by the liver and secreted in plasma.
Sequence similarities	Contains 4 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal Sushi
Ligand	Heparin-binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation, intrinsic pathway Inferred from electronic annotation. Source: Compara negative regulation of angiogenesis Inferred from electronic annotation. Source: Compara negative regulation of blood coagulation Inferred from electronic annotation. Source: Compara negative regulation of endothelial cell migration Inferred from electronic annotation. Source: Compara negative regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of fibrinolysis Inferred from electronic annotation. Source: Compara negative regulation of myeloid cell apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell apoptotic process Inferred from electronic annotation. Source: Compara plasminogen activation Inferred from electronic annotation. Source: Compara positive regulation of lipoprotein lipase activity Inferred from electronic annotation. Source: Compara regulation of blood coagulation Inferred from mutant phenotype PubMed 11145969. Source: MGI triglyceride metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara chylomicron Inferred from electronic annotation. Source: Compara extracellular matrix Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 11145969. Source: MGI high-density lipoprotein particle Inferred from electronic annotation. Source: Compara very-low-density lipoprotein particle Inferred from electronic annotation. Source: Compara
Molecular_function	eukaryotic cell surface binding Inferred from electronic annotation. Source: Compara heparin binding Inferred from electronic annotation. Source: UniProtKB-KW lipoprotein lipase activator activity Inferred from electronic annotation. Source: Compara phospholipid binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 345	326	Beta-2-glycoprotein 1		PRO_0000002060
Regions
	Domain	21 – 81	61	Sushi 1
	Domain	82 – 139	58	Sushi 2
	Domain	140 – 202	63	Sushi 3
	Domain	203 – 262	60	Sushi 4
	Region	263 – 345	83	Sushi-like
Amino acid modifications
	Glycosylation	105	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	117	1	N-linked (GlcNAc...) Ref.5 Ref.6
	Glycosylation	162	1	N-linked (GlcNAc...) Ref.5 Ref.6
	Glycosylation	183	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	193	1	N-linked (GlcNAc...) Ref.6
	Disulfide bond	23 ↔ 66		By similarity
	Disulfide bond	51 ↔ 79		By similarity
	Disulfide bond	84 ↔ 124		By similarity
	Disulfide bond	110 ↔ 137		By similarity
	Disulfide bond	142 ↔ 188		By similarity
	Disulfide bond	174 ↔ 200		By similarity
	Disulfide bond	205 ↔ 248		By similarity
	Disulfide bond	234 ↔ 260		By similarity
	Disulfide bond	264 ↔ 315		By similarity
	Disulfide bond	300 ↔ 325		By similarity
	Disulfide bond	307 ↔ 345		By similarity
Experimental info
	Sequence conflict	252	1	G → R in AAB30789. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q01339 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: C83F8A6EBD51C940	FASTA	345	38,619
	10 20 30 40 50 60 MVSPVLALFS AFLCHVAIAG RICPKPDDLP FATVVPLKTS YDPGEQIVYS CKPGYVSRGG 70 80 90 100 110 120 MRRFTCPLTG MWPINTLRCV PRVCPFAGIL ENGIVRYTSF EYPKNISFAC NPGFFLNGTS 130 140 150 160 170 180 SSKCTEEGKW SPDIPACARI TCPPPPVPKF ALLKDYRPSA GNNSLYQDTV VFKCLPHFAM 190 200 210 220 230 240 IGNDTVMCTE QGNWTRLPEC LEVKCPFPPR PENGYVNYPA KPVLLYKDKA TFGCHETYKL 250 260 270 280 290 300 DGPEEAECTK TGTWSFLPTC RESCKLPVKK ATVLYQGMRV KIQEQFKNGM MHGDKIHFYC 310 320 330 340 KNKEKKCSYT VEAHCRDGTI EIPSCFKEHS SLAFWKTDAS ELTPC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of mouse beta 2-glycoprotein I and mapping of the gene to chromosome 11." Nonaka M., Matsuda Y., Shiroishi T., Moriwak K., Natsuume-Sakai S. Genomics 13:1082-1087(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization, expression and evolution of mouse beta 2-glycoprotein I (apolipoprotein H)." Sellar G.C., Steel D.M., Zafiropoulos A., Seery L.T., Whitehead A.S. Biochem. Biophys. Res. Commun. 200:1521-1528(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CBA/J. Tissue: Liver.
[3]	"Structure of the human beta-2-glycoprotein I gene." Kristensen T. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[5]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-117 AND ASN-162, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[6]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-105; ASN-117; ASN-162; ASN-183 AND ASN-193, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10056 mRNA. Translation: BAA00945.1. S70439 mRNA. Translation: AAB30789.1. Y11356 mRNA. Translation: CAA72190.1. BC019811 mRNA. Translation: AAH19811.1. BC053338 mRNA. Translation: AAH53338.1.
IPI	IPI00322463.
PIR	NBMS. A43286.
RefSeq	NP_038503.4. NM_013475.4.
UniGene	Mm.2266.
3D structure databases
ProteinModelPortal	Q01339.
SMR	Q01339. Positions 20-345.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q01339. 1 interaction.
PTM databases
PhosphoSite	Q01339.
Proteomic databases
PaxDb	Q01339.
PRIDE	Q01339.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000049; ENSMUSP00000000049; ENSMUSG00000000049.
GeneID	11818.
KEGG	mmu:11818.
UCSC	uc007mbp.2. mouse.
Organism-specific databases
CTD	350.
MGI	MGI:88058. Apoh.
Phylogenomic databases
eggNOG	NOG323950.
GeneTree	ENSGT00580000081405.
HOGENOM	HOG000034008.
HOVERGEN	HBG004271.
InParanoid	Q01339.
OMA	SRPDNGF.
OrthoDB	EOG4V9TR1.
Gene expression databases
ArrayExpress	Q01339.
Bgee	Q01339.
CleanEx	MM_APOH.
Genevestigator	Q01339.
GermOnline	ENSMUSG00000000049. Mus musculus.
Family and domain databases
InterPro	IPR015104. Sushi_2. IPR000436. Sushi_SCR_CCP. [Graphical view]
Pfam	PF00084. Sushi. 4 hits. PF09014. Sushi_2. 1 hit. [Graphical view]
ProDom	PD012422. Sushi_2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00032. CCP. 5 hits. [Graphical view]
SUPFAM	SSF57535. Complement_control_module. 5 hits.
PROSITE	PS50923. SUSHI. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	279707.
SOURCE	Search...

Entry information

Entry name

APOH_MOUSE

Accession

Primary (citable) accession number: Q01339

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program