Q01338 (ADA2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-2A adrenergic receptor

Alternative name(s):
Alpha-2A adrenoreceptor
Short name=Alpha-2A adrenoceptor
Short name=Alpha-2AAR

Gene names

Name:

Adra2a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF2B1	Q14232	2	EBI-491073,EBI-491065	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Alpha-2A adrenergic receptor		PRO_0000069081
Regions
	Topological domain	1 – 33	33	Extracellular By similarity
	Transmembrane	34 – 59	26	Helical; Name=1; By similarity
	Topological domain	60 – 70	11	Cytoplasmic By similarity
	Transmembrane	71 – 96	26	Helical; Name=2; By similarity
	Topological domain	97 – 106	10	Extracellular By similarity
	Transmembrane	107 – 129	23	Helical; Name=3; By similarity
	Topological domain	130 – 149	20	Cytoplasmic By similarity
	Transmembrane	150 – 173	24	Helical; Name=4; By similarity
	Topological domain	174 – 192	19	Extracellular By similarity
	Transmembrane	193 – 217	25	Helical; Name=5; By similarity
	Topological domain	218 – 374	157	Cytoplasmic By similarity
	Transmembrane	375 – 399	25	Helical; Name=6; By similarity
	Topological domain	400 – 409	10	Extracellular By similarity
	Transmembrane	410 – 430	21	Helical; Name=7; By similarity
	Topological domain	431 – 450	20	Cytoplasmic By similarity
Sites
	Site	113	1	Implicated in ligand binding By similarity
	Site	200	1	Implicated in catechol agonist binding By similarity
	Site	204	1	Implicated in catechol agonist binding By similarity
Amino acid modifications
	Modified residue	297	1	Phosphoserine By similarity
	Modified residue	298	1	Phosphoserine By similarity
	Modified residue	299	1	Phosphoserine By similarity
	Lipidation	442	1	S-palmitoyl cysteine By similarity
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	14	1	N-linked (GlcNAc...) Potential
	Disulfide bond	106 ↔ 188		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01338 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F07E225393AFA93B	FASTA	450	48,865
	10 20 30 40 50 60 MGSLQPDAGN SSWNGTEAPG GGTRATPYSL QVTLTLVCLA GLLMLFTVFG NVLVIIAVFT 70 80 90 100 110 120 SRALKAPQNL FLVSLASADI LVATLVIPFS LANEVMGYWY FGKVWCEIYL ALDVLFCTSS 130 140 150 160 170 180 IVHLCAISLD RYWSITQAIE YNLKRTPRRI KAIIVTVWVI SAVISFPPLI SIEKKGAGGG 190 200 210 220 230 240 QQPAEPSCKI NDQKWYVISS SIGSFFAPCL IMILVYVRIY QIAKRRTRVP PSRRGPDACS 250 260 270 280 290 300 APPGGADRRP NGLGPERGAG PTGAEAEPLP TQLNGAPGEP APAGPRDGDA LDLEESSSSE 310 320 330 340 350 360 HAERPPGPRR PDRGPRAKGK TRASQVKPGD SLPRRGPGAA GPGASGSGHG EERGGGAKAS 370 380 390 400 410 420 RWRGRQNREK RFTFVLAVVI GVFVVCWFPF FFTYTLIAVG CPVPSQLFNF FFWFGYCNSS 430 440 450 LNPVIYTIFN HDFRRAFKKI LCRGDRKRIV

References

[1]	"Cloning of two mouse genes encoding alpha 2-adrenergic receptor subtypes and identification of a single amino acid in the mouse alpha 2-C10 homolog responsible for an interspecies variation in antagonist binding." Link R.E., Daunt D.A., Barsh G., Chruscinski A.J., Kobilka B.K. Mol. Pharmacol. 42:16-27(1992) [PubMed: 1353249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M99377 Genomic DNA. Translation: AAA37213.1.
IPI	IPI00122276.
PIR	I49481.
UniGene	Mm.235195.
3D structure databases
ProteinModelPortal	Q01338.
SMR	Q01338. Positions 37-443.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q01338. 1 interaction.
STRING	Q01338.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q01338.
Proteomic databases
PRIDE	Q01338.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:87934. Adra2a.
Phylogenomic databases
eggNOG	maNOG17874.
HOVERGEN	HBG106962.
InParanoid	Q01338.
OrthoDB	EOG4R502W.
PhylomeDB	Q01338.
Gene expression databases
ArrayExpress	Q01338.
Bgee	Q01338.
CleanEx	MM_ADRA2A.
Genevestigator	Q01338.
GermOnline	ENSMUSG00000033717. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR001946. Adren_rcpt_A2A. IPR002233. Adrnrgc_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01103. ADRENERGICR. PR00558. ADRENRGCA2AR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279038.
SOURCE	Search...

Entry information

Entry name

ADA2A_MOUSE

Accession

Primary (citable) accession number: Q01338

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 16, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program