Q01329 (PTA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-tRNA-processing protein PTA1

Gene names

Name:	PTA1
Ordered Locus Names:	YAL043C
ORF Names:	FUN39

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential in pre-tRNA processing. Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation.
Subunit structure	Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2. Ref.4
Subcellular location	Nucleus Ref.4.
Miscellaneous	Present with 3730 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	mRNA processing tRNA processing
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA cleavage Inferred from mutant phenotype PubMed 10523662. Source: SGD mRNA polyadenylation Inferred from mutant phenotype PubMed 10523662PubMed 9303317. Source: SGD tRNA processing Inferred from mutant phenotype Ref.1. Source: SGD termination of RNA polymerase II transcription, exosome-dependent Inferred from physical interaction Ref.4. Source: SGD termination of RNA polymerase II transcription, poly(A)-coupled Inferred from physical interaction Ref.4. Source: SGD
Cellular_component	mRNA cleavage and polyadenylation specificity factor complex Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 785	785	Pre-tRNA-processing protein PTA1		PRO_0000097086
Amino acid modifications
	Modified residue	378	1	Phosphothreonine Ref.6
	Modified residue	380	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q01329 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: E2AB1F468B7BC8D0	FASTA	785	88,473
	10 20 30 40 50 60 MSSAEMEQLL QAKTLAMHNN PTEMLPKVLE TTASMYHNGN LSKLKLPLAK FFTQLVLDVV 70 80 90 100 110 120 SMDSPIANTE RPFIAAQYLP LLLAMAQSTA DVLVYKNIVL IMCASYPLVL DLVAKTSNQE 130 140 150 160 170 180 MFDQLCMLKK FVLSHWRTAY PLRATVDDET DVEQWLAQID QNIGVKLATI KFISEVVLSQ 190 200 210 220 230 240 TKSPSGNEIN SSTIPDNHPV LNKPALESEA KRLLDMLLNY LIEEQYMVSS VFIGIINSLS 250 260 270 280 290 300 FVIKRRPQTT IRILSGLLRF NVDAKFPLEG KSDLNYKLSK RFVERAYKNF VQFGLKNQII 310 320 330 340 350 360 TKSLSSGSGS SIYSKLTKIS QTLHVIGEET KSKGILNFDP SKGNSKKTLS RQDKLKYISL 370 380 390 400 410 420 WKRQLSALLS TLGVSTKTPT PVSAPATGSS TENMLDQLKI LQKYTLNKAS HQGNTFFNNS 430 440 450 460 470 480 PKPISNTYSS VYSLMNSSNS NQDVTQLPND ILIKLSTEAI LQMDSTKLIT GLSIVASRYT 490 500 510 520 530 540 DLMNTYINSV PSSSSSKRKS DDDDDGNDNE EVGNDGPTAN SKKIKMETEP LAEEPEEPED 550 560 570 580 590 600 DDRMQKMLQE EESAQEISGD ANKSTSAIKE IAPPFEPDSL TQDEKLKYLS KLTKKLFELS 610 620 630 640 650 660 GRQDTTRAKS SSSSSILLDD DDSSSWLHVL IRLVTRGIEA QEASDLIREE LLGFFIQDFE 670 680 690 700 710 720 QRVSLIIEWL NEEWFFQTSL HQDPSNYKKW SLRVLESLGP FLENKHRRFF IRLMSELPSL 730 740 750 760 770 780 QSDHLEALKP ICLDPARSSL GFQTLKFLIM FRPPVQDTVR DLLHQLKQED EGLHKQCDSL LDRLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing." O'Connor J.P., Peebles C.L. Mol. Cell. Biol. 12:3843-3856(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends." Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J. J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CPF COMPLEX, COMPOSITION OF THE APT COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-378 AND THR-380, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95673 Genomic DNA. Translation: AAA34919.1. U12980 Genomic DNA. Translation: AAC04988.1. BK006935 Genomic DNA. Translation: DAA06943.1.
PIR	S31299.
RefSeq	NP_009356.1. NM_001178188.1.
3D structure databases
ProteinModelPortal	Q01329.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2469N.
IntAct	Q01329. 28 interactions.
MINT	MINT-375412.
STRING	4932.YAL043C.
Proteomic databases
PaxDb	Q01329.
PeptideAtlas	Q01329.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL043C; YAL043C; YAL043C.
GeneID	851255.
KEGG	sce:YAL043C.
Organism-specific databases
CYGD	YAL043c.
SGD	S000000041. PTA1.
Phylogenomic databases
eggNOG	NOG322107.
HOGENOM	HOG000115754.
KO	K06100.
OMA	EWLSEEW.
OrthoDB	EOG4DNJCW.
Gene expression databases
Genevestigator	Q01329.
GermOnline	YAL043C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021850. DUF3453. [Graphical view]
Pfam	PF11935. DUF3453. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968207.

Entry information

Entry name

PTA1_YEAST

Accession

Primary (citable) accession number: Q01329
Secondary accession number(s): D6VPH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program