Q01319 (RIR2_BHV1C) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase small chain
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

Name:

UL40

Organism

Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)

Taxonomic identifier

10323 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus › Bovine herpesvirus 1 › Bovine herpesvirus type 1.1

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small chain.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Ribonucleoside-diphosphate reductase small chain		PRO_0000190507
Sites
	Active site	110	1	By similarity
	Metal binding	73	1	Iron 1 By similarity
	Metal binding	103	1	Iron 1 By similarity
	Metal binding	103	1	Iron 2 By similarity
	Metal binding	106	1	Iron 1 By similarity
	Metal binding	166	1	Iron 2 By similarity
	Metal binding	200	1	Iron 2 By similarity
	Metal binding	203	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q01319 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 257FB0E91111632E	FASTA	314	35,251
	10 20 30 40 50 60 MAEAADAATL TRKYKYFYET ECPDLDHLRS LSVANRWLET EFPLADDAKD VARLSGAELE 70 80 90 100 110 120 FYRFLFAFLS AADDLVNVNL GDLSELFTQK DILHYYIEQE SIEVVHSRVY SAIQLLLFRN 130 140 150 160 170 180 DAVARAGYVE GALGDPAVRR KVDWLERRVA AAESVAEKYV LMILIEGIFF SSSFAAIAYL 190 200 210 220 230 240 RTHNLFVVTC QTNDLISRDE AVHTAASCCI FDNYLGGERP PPARIYELFR EAWKLSASLF 250 260 270 280 290 300 GCAPRGSHIL DVEAISAYVE YSADRLLAAI QLPPLFGTPP PGTDFPLALM TAEKHTNFFE 310 RRSTNYTGTV INDL

References

[1]	"Sequencing and 5'- and 3'-end transcript mapping of the gene encoding the small subunit of ribonucleotide reductase from bovine herpesvirus type-1." Simard C., Bastien N., Trudel M. Virology 190:689-701(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Cooper / 34.
[2]	"Sequence analysis of the UL39, UL38, and UL37 homologues of bovine herpesvirus 1 and expression studies of UL40 and UL39, the subunits of ribonucleotide reductase." Simard C., Langlois I., Styger D., Vogt B., Vlcek C., Chalifour A., Trudel M., Schwyzer M. Virology 212:734-740(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Gene contents in a 31-kb segment at the left genome end of bovine herpesvirus-1." Schwyzer M., Styger D., Vogt B., Lowery D.E., Simard C., LaBoissiere S., Misra V., Vlcek C., Paces V. Vet. Microbiol. 53:67-77(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Tinkering with a viral ribonucleotide reductase." Lembo D., Brune W. Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z54206 Genomic DNA. Translation: CAA90928.1. M84470 Genomic DNA. Translation: AAA46063.1. Z49078 Genomic DNA. Translation: CAA88899.1. AJ004801 Genomic DNA. Translation: CAA06093.1.
PIR	WMBEB4. A43367.
RefSeq	NP_045318.1. NC_001847.1.
3D structure databases
ProteinModelPortal	Q01319.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4783423.
Phylogenomic databases
ProtClustDB	CLSP2509600.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_BHV1C

Accession

Primary (citable) accession number: Q01319

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 29, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program