Reviewed,
UniProtKB/Swiss-Prot Q01314 (AKT1_BOVIN)
Last modified
February 9, 2010.
Version 94.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: RAC-alpha serine/threonine-protein kinase EC=2.7.11.1 Alternative name(s): RAC-PK-alpha Protein kinase B Short name=PKB | ||||
| Gene names |
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| Organism | Bos taurus (Bovine) | ||||
| Taxonomic identifier | 9913 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 480 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2, thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | The C-terminus interacts with the C-terminus of CCDC88A/GRDN and THEM4. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with TRAF6 By similarity. |
| Subcellular location | Cytoplasm By similarity. Nucleus By similarity. Cell membrane By similarity. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A By similarity. |
| Domain | Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in its targeting to the plasma membrane. The AGC-kinase C-terminal mediates interaction with THEM4 By similarity. |
| Post-translational modification | Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1 By similarity. Ser-473 is dephosphorylated by PHLPP. Ref.4 Polyubiquitinated. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Ppl | Q9R269 | 1 | EBI-368344,EBI-368293 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 480 | 480 | RAC-alpha serine/threonine-protein kinase | PRO_0000085604 | |||||
Regions | |||||||||
| Domain | 5 – 108 | 104 | PH | ||||||
| Domain | 150 – 408 | 259 | Protein kinase | ||||||
| Domain | 409 – 480 | 72 | AGC-kinase C-terminal | ||||||
| Nucleotide binding | 156 – 164 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 274 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 179 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 124 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 129 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 308 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 473 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 474 | 1 | Phosphotyrosine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 149 | 1 | D → E in CAA43371. Ref.1 | ||||||
| Sequence conflict | 153 | 1 | L → V in CAA43371. Ref.1 | ||||||
| Sequence conflict | 173 – 174 | 2 | GR → AA in CAA43371. Ref.1 | ||||||
| Sequence conflict | 209 | 1 | F → S in CAA43371. Ref.1 | ||||||
Sequences
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References
| [1] | "Molecular cloning and characterisation of a novel putative protein-serine kinase related to the cAMP-dependent and protein kinase C families." Coffer P.J., Woodgett J.R. Eur. J. Biochem. 201:475-481(1991) [PubMed: 1718748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [2] | Erratum Coffer P.J., Woodgett J.R. Eur. J. Biochem. 205:1217-1218(1992) [PubMed: 1533586] [Abstract] Cited for: SEQUENCE REVISION. |
| [3] | "Complete sequence of the bovine AKT1 gene." Khatib H. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [4] | "PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth." Gao T., Furnari F., Newton A.C. Mol. Cell 18:13-24(2005) [PubMed: 15808505] [Abstract] Cited for: DEPHOSPHORYLATION AT SER-473 BY PHLPP. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X61036 mRNA. Translation: CAA43371.1. AY781100 mRNA. Translation: AAW71957.1. |
| IPI | IPI00696205. |
| PIR | S62117. |
| RefSeq | NP_776411.1. |
| UniGene | Bt.4843 |
3D structure databases | |
| SMR | Q01314. Positions 3-121, 144-478. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q01314. 1 interaction. |
| STRING | Q01314. |
Genome annotation databases | |
| Ensembl | ENSBTAT00000023461; ENSBTAP00000023461; ENSBTAG00000017636; Bos taurus. [Genome view] |
| GeneID | 280991. |
| KEGG | bta:280991. |
Organism-specific databases | |
| CTD | 280991. |
Phylogenomic databases | |
| eggNOG | maNOG18684. |
| HOVERGEN | Q01314. |
| PhylomeDB | Q01314. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 251. |
Family and domain databases | |
| InterPro | IPR000961. AGC-kinase_C. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR017892. Pkinase_C. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. IPR015744. Serine/threonine_Kinase_Rac. [Graphical view] |
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. |
| PANTHER | PTHR22985:SF69. Akt. 1 hit. |
| Pfam | PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view] |
| SMART | SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AKT1_BOVIN | ||||||||
| Accession | Primary (citable) accession number: Q01314 Secondary accession number(s): Q5ER96 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


