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Protein

Broad-complex core protein isoforms 1/2/3/4/5

Gene

br

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Broad-complex proteins are required for puffing and transcription of salivary gland late genes during metamorphosis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri636 – 659C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri666 – 689C2H2-type 2PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: FlyBase
  • transcription factor activity, sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • autophagy Source: FlyBase
  • cell death Source: FlyBase
  • cellular response to ecdysone Source: FlyBase
  • compound eye photoreceptor fate commitment Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • ecdysone-mediated induction of salivary gland cell autophagic cell death Source: FlyBase
  • eclosion Source: FlyBase
  • gravitaxis Source: FlyBase
  • male genitalia morphogenesis Source: FlyBase
  • metamorphosis Source: FlyBase
  • muscle fiber development Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • oogenesis Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • progression of morphogenetic furrow involved in compound eye morphogenesis Source: FlyBase
  • regulation of development, heterochronic Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • response to ecdysone Source: FlyBase
  • response to symbiont Source: FlyBase
  • salivary gland histolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ01295.

Names & Taxonomyi

Protein namesi
Recommended name:
Broad-complex core protein isoforms 1/2/3/4/5
Gene namesi
Name:br
Synonyms:Br-C
ORF Names:CG11491
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0283451. br.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000470691 – 727Broad-complex core protein isoforms 1/2/3/4/5Add BLAST727

Proteomic databases

PaxDbiQ01295.
PRIDEiQ01295.

Expressioni

Developmental stagei

Isoforms Z1 accumulate slowly in mid instar larvae salivary glands at beginning of ecdysone response (94-114 hours of development at puff stage 1) and become the predominant isoform after 6 hours. Levels diminish at puff stage 2 and are moderately abundant in late larvae from stages 3-10. In prepupae, transcripts appear at puff stage 15 onwards, reaching maximum at stages 18-20. In gut, levels remain constant between stages 1-11. In Malpighian tubules, Z1 isoforms are seen at stages 3 and 7, but not at stage 11. In fat body and wing disks, low levels increase between stages 3 and 11. Isoform Z2 accumulates to a high level at the beginning of the ecdysone response during puff stage 1 and abruptly disappears after several hours. In prepupae, transcripts are reinduced at low levels. Low levels are seen in the Malpighian Tubules, gut and fat body between stages 1-11 and high levels in the wing disk. Isoform Z3; in mid instar larval salivary gland transcript accumulates to a high level at the beginning of the ecdysone response, 94-98 hours of development in puff stage 1, and abruptly disappears after several hours. Levels increase by puff stage 3 remaining abundant in late larvae until stage 10, then diminish by stage 11. In prepupae, transcripts are abundant and increase during puff stages 11-14 and 18-20. High levels are seen in Malpighian tubules, gut and fat body between stages 1-11 and low levels in wing disk.2 Publications

Inductioni

Primary response to 20-hydroxyecdysone in third instar larval imaginal disks.

Gene expression databases

BgeeiFBgn0000210.
GenevisibleiQ01295. DM.

Interactioni

Protein-protein interaction databases

BioGridi69091. 11 interactors.
STRINGi7227.FBpp0303432.

Structurei

3D structure databases

ProteinModelPortaliQ01295.
SMRiQ01295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 97BTBPROSITE-ProRule annotationAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi203 – 207Poly-Ala5
Compositional biasi265 – 268Poly-Asn4
Compositional biasi439 – 444Poly-Thr6
Compositional biasi451 – 460Poly-Asn10
Compositional biasi479 – 490Poly-ThrAdd BLAST12
Compositional biasi533 – 543Poly-GlnAdd BLAST11
Compositional biasi546 – 558Poly-GlnAdd BLAST13
Compositional biasi565 – 568Poly-Gln4
Compositional biasi596 – 603Poly-Ala8
Compositional biasi714 – 720Poly-Gln7

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri636 – 659C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri666 – 689C2H2-type 2PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IVCP. Eukaryota.
ENOG410YRXI. LUCA.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q01295-1) [UniParc]FASTAAdd to basket
Also known as: BRCORE-TNT1-Q1-Z1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDTQHFCLR WNNYQSSITS AFENLRDDEA FVDVTLACEG RSIKAHRVVL
60 70 80 90 100
SACSPYFREL LKSTPCKHPV ILLQDVNFMD LHALVEFIYH GEVNVHQKSL
110 120 130 140 150
QSFLKTAEVL RVSGLTQQQA EDTHSHLAQI QNLANSGGRT PLNTHTQSLP
160 170 180 190 200
HPHHGSLHDD GGSSTLFSRQ GAGSPPPTAV PSLPSHINNQ LLKRMAMMHR
210 220 230 240 250
SSAAAAAEET SHAFKRLRGS DNSLPLSGAV GSGSNNNSPD LPPLHARSAS
260 270 280 290 300
PQQTPADFST IKHHNNNNTP PLKEEKRNGP TGNGNSGNGN GNGNGASNGN
310 320 330 340 350
GISISDKLGS LTPSPLARAG ADDVKSEPMD MVCSNNNANA NDEHSNDSTG
360 370 380 390 400
EHDANRSSSG DGGKGSLSSG NDEEIGDGLA SHHAAPQFIM SPAENKMFHA
410 420 430 440 450
AAFNFPNIDP SALLGLNTQL QQSGDLAVSP QGKLTTNATT TTTTINNSIT
460 470 480 490 500
NNNNNNNNNN YDYSLPTKNS NSQKTPSPTT TTLTTPTTTT PTRPTAITSA
510 520 530 540 550
SGICGLNLST FAANGSSSGG SNGGLSMTAL LPQQQQQQQQ QHQMSQQQQQ
560 570 580 590 600
QQQQQQQQGN SSSGQQQQPN GILACSTPKA NTPTTTQQQM YAAVMAAAAS
610 620 630 640 650
ASASTSGSAN SSLNNSNSTL NTSGGLNNSA SGGDDFRCNP CNKNLSSLTR
660 670 680 690 700
LKRHIQNVHM RPTKEPVCNI CKRVYSSLNS LRNHKSIYHR NLKQPKQEPG
710 720
VGATQAAANS FYHQQHQQQQ LNHHSSS
Length:727
Mass (Da):77,393
Last modified:February 21, 2001 - v2
Checksum:i97BBA20F3BD734D6
GO
Isoform 2 (identifier: Q01295-2) [UniParc]FASTAAdd to basket
Also known as: BRCORE-Q1-Z1

The sequence of this isoform differs from the canonical sequence as follows:
     432-495: Missing.

Show »
Length:663
Mass (Da):70,534
Checksum:iA91201DDCE557FD6
GO
Isoform 3 (identifier: Q01295-3) [UniParc]FASTAAdd to basket
Also known as: BRCORE-Q2-Z1

The sequence of this isoform differs from the canonical sequence as follows:
     432-478: Missing.

Show »
Length:680
Mass (Da):72,308
Checksum:i3D10B493DA14A9CD
GO
Isoform 4 (identifier: Q01295-4) [UniParc]FASTAAdd to basket
Also known as: BRCORE-Z2

The sequence of this isoform differs from the canonical sequence as follows:
     433-514: KLTTNATTTT...GLNLSTFAAN → NSPKKLFSCQ...KLYNQFNSSI
     515-727: Missing.

Show »
Length:514
Mass (Da):55,459
Checksum:i78160AB1B326CDD7
GO
Isoform 5 (identifier: Q01295-5) [UniParc]FASTAAdd to basket
Also known as: BRCORE-NS-Z3

The sequence of this isoform differs from the canonical sequence as follows:
     433-727: KLTTNATTTT...QQQLNHHSSS → PHSITRSAAT...ELGQPPPGIQ

Show »
Length:702
Mass (Da):74,145
Checksum:iB1A40D785201B832
GO
Isoform 6 (identifier: Q24206-1) [UniParc]FASTAAdd to basket
Also known as: BRCORE-Z4
The sequence of this isoform can be found in the external entry Q24206.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:880
Mass (Da):92,305
GO

Sequence cautioni

The sequence CAA15629 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti230V → L in CAA38477 (PubMed:1743483).Curated1
Sequence conflicti230V → L in CAA38474 (PubMed:1743483).Curated1
Sequence conflicti230V → L in CAA38475 (PubMed:1743483).Curated1
Sequence conflicti457 – 459Missing (PubMed:10731132).Curated3
Sequence conflicti460N → NN in CAA38477 (PubMed:1743483).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006836432 – 495Missing in isoform 2. CuratedAdd BLAST64
Alternative sequenceiVSP_006835432 – 478Missing in isoform 3. CuratedAdd BLAST47
Alternative sequenceiVSP_006839433 – 727KLTTN…HHSSS → PHSITRSAATSPTSSTSSPP SPPTALISPTSSLKGSLAAA VYSLHSHAHGHVLGHATSPP RPGSVGSSVGSNLCTSTSMG CGVNSGNNSGNNNGNNANNN SNNGNATNNNNNSSSSSTSP GSQATAAGGTVTQAGTPPLP LRMPPPTSGGINEPQECPYC RRTFSCYYSLKRHFQDKHEQ SDTLYVCEFCHRRYRTKNSL TTHKSLQHRGSSGMLKRLLK TTAIKHGLVGHGHGHGHVHH PHAHHHALSHPRTSLYDFTS ELGQPPPGIQ in isoform 5. CuratedAdd BLAST295
Alternative sequenceiVSP_006837433 – 514KLTTN…TFAAN → NSPKKLFSCQLCGKLLCSKA SLKRHIADKHAVRQEEYRCA ICERVYCSRNSLMTHIYTYH KSRPGEMEMKDIKLYNQFNS SI in isoform 4. CuratedAdd BLAST82
Alternative sequenceiVSP_006838515 – 727Missing in isoform 4. CuratedAdd BLAST213

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54666 mRNA. Translation: CAA38477.1.
X54665 mRNA. Translation: CAA38476.1.
X54663 mRNA. Translation: CAA38474.1.
X54664 mRNA. Translation: CAA38475.1.
AE014298 Genomic DNA. Translation: AAF45648.1.
AE014298 Genomic DNA. Translation: AAF45651.2.
AE014298 Genomic DNA. Translation: AAN09054.1.
AL009146 Genomic DNA. Translation: CAA15629.1. Sequence problems.
AL009146 Genomic DNA. Translation: CAA15626.1.
AL009146 Genomic DNA. Translation: CAA15628.1.
PIRiS21912.
S21913.
S21914.
RefSeqiNP_001188525.1. NM_001201596.3. [Q01295-4]
NP_001188526.1. NM_001201597.1.
NP_001188527.1. NM_001201598.1.
NP_001245462.1. NM_001258533.1. [Q01295-2]
NP_524759.2. NM_080020.4.
NP_726749.2. NM_166893.2. [Q01295-4]
NP_726750.1. NM_166894.4. [Q01295-5]
NP_726754.1. NM_166898.2. [Q01295-2]
UniGeneiDm.20757.

Genome annotation databases

GeneIDi44505.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54666 mRNA. Translation: CAA38477.1.
X54665 mRNA. Translation: CAA38476.1.
X54663 mRNA. Translation: CAA38474.1.
X54664 mRNA. Translation: CAA38475.1.
AE014298 Genomic DNA. Translation: AAF45648.1.
AE014298 Genomic DNA. Translation: AAF45651.2.
AE014298 Genomic DNA. Translation: AAN09054.1.
AL009146 Genomic DNA. Translation: CAA15629.1. Sequence problems.
AL009146 Genomic DNA. Translation: CAA15626.1.
AL009146 Genomic DNA. Translation: CAA15628.1.
PIRiS21912.
S21913.
S21914.
RefSeqiNP_001188525.1. NM_001201596.3. [Q01295-4]
NP_001188526.1. NM_001201597.1.
NP_001188527.1. NM_001201598.1.
NP_001245462.1. NM_001258533.1. [Q01295-2]
NP_524759.2. NM_080020.4.
NP_726749.2. NM_166893.2. [Q01295-4]
NP_726750.1. NM_166894.4. [Q01295-5]
NP_726754.1. NM_166898.2. [Q01295-2]
UniGeneiDm.20757.

3D structure databases

ProteinModelPortaliQ01295.
SMRiQ01295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69091. 11 interactors.
STRINGi7227.FBpp0303432.

Proteomic databases

PaxDbiQ01295.
PRIDEiQ01295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi44505.

Organism-specific databases

CTDi103946.
FlyBaseiFBgn0283451. br.

Phylogenomic databases

eggNOGiENOG410IVCP. Eukaryota.
ENOG410YRXI. LUCA.

Enzyme and pathway databases

SignaLinkiQ01295.

Miscellaneous databases

ChiTaRSiBr. fly.
GenomeRNAii44505.

Gene expression databases

BgeeiFBgn0000210.
GenevisibleiQ01295. DM.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRC1_DROME
AccessioniPrimary (citable) accession number: Q01295
Secondary accession number(s): O46065
, O46066, Q01293, Q01296, Q9W571, Q9W574
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 21, 2001
Last modified: November 30, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.